SFE

Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line

midiprep kit Stratagene EF plasmid midiprep kit, a kit based on anion exchange chromatography, magneticbeads, and cesium ch...

Optimizing Transfection Conditions for Studying Signal Transduction Pathways

Optimizing Transfection Conditions for Studying Signal TransductionPathways in vivo reporting systems Stratagene Stratagenes PathDetect <font face="S...

Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells

Department of Cell Biology, Neurobiology, and Anatomy University of Cincinnati College of Medicine, Cincinnati, OH 45267 Transfection of mammalian cell lines is enhanced by the use ofliposome-based reagents. Here we compare the transfection e...

Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts

reagent Genetic Applications LLC Stratagene transfection reagentpromotes efficient transfection of human dermal fibroblasts. Twenty minutes...

Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance

Stratagene transfection reagent offersimproved, consistent transfections for both adherent cells and cells growing insuspension. In this article, we summarize the results of several experimentsthat characterize the eff...

Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells

Stratagene Cloning Systems Clonetics Corporation, Walkersville, Maryland LipoTAXI transfection reagent is a novel liposome reagent that offerslow cellular toxicity and high transfection efficiencies for adhe...

Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells

Mycoplasma PCR Primer Set for fast and easy detection of mycoplasmacontamination Yale University Genetic Applications LLC Stratagene Mycoplasma contamination of mammalian cell cultures can substantiallyreduce the potenc...

High-Efficiency Transfections Achieved with New Low-Toxicity Reagent

Nonliposomal formulation yields high efficiencies in a variety oftransfection conditions Stratagene Introducing foreign DNA into eukaryotic cells requires high-potencytransfections, which demand superior quality transfection reagents that areefficient, have low toxicity, and wor...

Outstanding transfection rates using the Multiporator

Using eukaryotic cells, extremely high transfection rates can be achieved due to a hypoosmolar buffer system and short pulses in the s-range. High reproducibility is ensured by an electronically controlled pulse, independent of sample resistance (patent pending)....

Efficient Transfection of Neurospora Crassa

Katherine A. Borkovich PhD, University of Texas-Houston Medical Center, Dept. of Microbiology and Molecular Genetics, Houston, Texas. Kimberly Vollmer, Brinkmann™ Instruments Inc., BioSystems Application Lab, Westbury, New York....

Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells

<A href="http://www.biocompare.com/techart.asp?id=575">Actinobacillus pleuropnomoniae <A href="http://www.biocompare.com/techart.asp?id=576">Actinomyces viscosus <A...

Outstanding transfection rates using the Multiporator

Using eukaryotic cells, extremely high transfection rates can be achieved due to a hypoosmolar buffer system and short pulses in the s-range. High reproducibility is ensured by an electronically controlled pulse, independent of sample resistance (patent pending)....

Efficient Transfection of Neurospora Crassa

Katherine A. Borkovich PhD, University of Texas-Houston Medical Center, Dept. of Microbiology and Molecular Genetics, Houston, Texas. Kimberly Vollmer, Brinkmann™ Instruments Inc., BioSystems Application Lab, Westbury, New York....

Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells

<A href="http://www.biocompare.com/techart.asp?id=575">Actinobacillus pleuropnomoniae <A href="http://www.biocompare.com/techart.asp?id=576">Actinomyces viscosus <A...

TransMessenger Transfection Reagent

For transfection of cells with siRNA in RNAi procedures Tested for a...

Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures

Jochen Meier, Ivonne Strmel, Radu Iosub, Sonja Schmidt, and Rosemarie Grantyn Developmental Physiology, Johannes Mller Institute, Humboldt University Medical School (Charit), Berlin, Germany Efficient delivery of DNA to primary neuronal cell cultures is of critical importance...

Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent

QIAGEN GmbH, Hilden, Germany Primary neurons are extremely difficult to transfect, making genetic modification of neuronal cells by delivery of either siRNA or plasmid DNA challenging ( ). Recently, Krichevsky and Kosik report...

Effectene Transfection Reagent

For transfection of a wide variety of cells, particularly effective for primary cells <l...

PolyFect Transfection Reagent

Optimized for transfection of COS-7, NIH/3T3, HeLa, 293, and CHO cells...

SuperFect Transfection Reagent

The proven activated dendrimer for transfection of a broad range of cell lines Ser...

TransMessenger Transfection Reagent Handbook

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Transfection Reagent Selector Kit Handbook

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TransMessenger Transfection Reagent

For transfection of cells with siRNA in RNAi procedures Tested for a...

Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*

Center for Neurologic Diseases, Brigham and Womens Hospital, Harvard Medical School, Boston, MA, USA Genetic manipulations using neuronal cells are notoriously difficult. Therefore, efficient delivery of short interfering RNA (siRNA)...

Sensenbrenner and USPTO Director highlight benefits of technology transfer

Hartland, Wis. At a meeting Monday highlighting university-business partnerships in Wisconsin, , a medical imaging company based in Hartland, Wis., demonstrated its latest tool for assessing cardiovascular health to John Dudas, acting under secretary of commerce for the <a href="http://www.uspto.gov/" target=...

CATI: Transforming technology transfer in Wisconsin

Racine, Wis. When people think of technology transfer in Wisconsin, they may think of the ; the two premier universitybased technology transfer programs in the state. But now theres a new player in town, bringing businesses and...

Uses and Applications of X-tremeGENE Q2 Transfection Reagent

Despite the wide spectrum of cell types that can be transfected using currently available transfection reagents, there are significant differences in the efficiency of DNA delivery into different types of cell. In certain cell types, such as HeLa, K-562, and Jurkat, the broad-sp...

General Considerations for Successful Transfection Experiments

Keep an eye on your cells; ensure they are in good condition and set a suitable plating protocol for optimal cell density from the beginning to the end of transfection....

Uses and Applications of FuGENE 6 Transfection Reagent

Troubleshooting Hints if You Observe Cytotoxicity (greater than 5 - 10 % Cell Death) FuGENE 6 Transfection Reagent has proven to be virtually nontoxic to most cell types. If you observe more than 5 - 10 % cell death in transient transfection experime...

Uses and Applications of FuGENE 6 Transfection Reagent

RNA interference induced by siRNAs or shRNAs allows reliable down-regulation of the expression of specific genes in human and other mammalian cells. While s...

Uses and Applications of FuGENE 6 Transfection Reagent

Nucleic acids of poor quality or insufficien...

Uses and Applications of FuGENE 6 Transfection Reagent

First, verify that both of your DNA constructs work in your cell line. Also, determine the ratio of DNA to FuGENE 6 Reagent (for example, 1 g of DNA and 3 l FuGENE 6 Reag...

Uses and Applications of FuGENE 6 Transfection Reagent

Below, a standard protocol for the DNA transfection of cultured cells using FuGENE 6 is presented that has been specially designed for scientists who are new to transfection....

Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer andthe Cell Fluorescence LabChip kit can be used to determine the efficiencyof transfection of mammalian cells using green fluorescent protein(GFP) as a reporter molecule. Transfection of CHO-K1 cells with...

Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection

In addition to the specificity andefficiency of an siRNA sequencefor a target gene, which are ofhigh importance in post-transcriptionalgene silencing (PTGS), thedelivery of siRNA into cells is crucial.An efficient method to optimizetransfection and gene silenci...

siRNA transfection optimization with the Agilent 2100 bioanalyzer

When working in the field of target validation, or when studying functionalassays requiring the transfection of small interfering RNA (siRNA)into a cell, the presence of having a fast, easy-to-use method to optimizetransfection conditions for given cell lines would be of g...

Choose the Right Transfection Agent for Your RNAi Experiment

The success of any RNA interference experiment depends on the efficient delivery of siRNA or siRNA expression vehicles (plasmids, PCR fragments) into cells. Many researchers use cationic liposomal and polyamine based reagents to deliver these molecules into cultured mammalian c...

Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection

(Research Associate II, Ambion, Inc.) Gene silencing using small interfering RNAs (siRNAs) has become a powerful method for studying gene function. Human primary cells are often desired for such experiments because they are mor...

Optimizing Chemical Transfection and Electroporation of siRNAs

(Associate Scientist, Ambion, Inc.) (Vice President and CSO, Cyto Pulse Sciences, Inc.) Low transfection efficiency is the most frequent cause of unsuccessful gene silencing...

Optimizing siRNA Transfection for RNAi

Low transfection efficiency and low cell viability are the most frequent causes of unsuccessful gene silencing experiments. Through careful optimization--e.g. choosing the right transfection agent and transfection method--high levels of transfection efficiency can be achieved...