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New Microarray Labeling Kit for Preparing cDNA Probes

A more reliable and reproducible way to create fluorescently orradioactively labeled cDNA David T. Wong Heidi Davis Darryl Garrison TerryPayette Matt Lundy Kim Kuplent Mary A. Buchanan Joseph A. Sorge Rebecca L. Mullinax Stratagene Phenogenex Nucleic acid micro...

Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems

Assess the In Vivo Activation of Signal Transduction Pathways withPathDetect Stratagene Signal transduction is essential to many cellular processes, such asgrowth, differentiation, an...

New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter

hrGFP expression vectors for monitoring gene expression and localizing proteins in vivo New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter...

A Simple and Rapid Technique to Purify High-Quality Midiprep DNA

Stratagene A new technique is now available to quickly, easily, and reliably isolatehigh-purity plasmid DNA. The convenient spin-format eliminates the need forresin suspensions, gravity flow columns, and alcohol precipit...

RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

Stratagene Cloning Systems from the cDNAsequences of mismatch repair genes. These genes code for specialized proteinsthat participate in correcting areas of base mispairing in DNA. We have...

Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements

Stratagene Cloning Systems, Inc. Enhancer elements are the convergent points for many intracellular signaltransduction pathways. For simple, rapid and convenient assessment of the invivo activation of these pathways, Stratagene has d...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acidpurification kits Stratagene Cloning Systems, Inc. Stratagene announces a new line of DNA purification products, whichprovide reliable isolation of high-purity miniprep plasmid, PCR product andgel-fractionate...

Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators

Lisa Hexdall Li Xu Alan Greener Mary Buchanan John Bauer Chao-Feng Zheng Stratagene HLR-CREB, HLR-Elk1, and HLR-cJun, cell lines for...

Versatile Reporter Vectors for Monitoring Viral Transduction

Determine retroviral transduction efficiency and gene expression invirtually any target cell line Stratagene , pFB-Neo-LacZ <font...

In Vivo Signal Transduction Pathway Reporting Systems

Stratagene Cloning Systems, Inc. Stratagene's PathDetect in vivo signal transduction pathway reportingsystems are designed for specific, rapid and convenient assessment of the invivo activation of signal transduction pathways. These r...

Improved Vectors for PathDetect Trans-Reporting Systems

Stratagene Stratagenes PathDetect in vivo signal transduction pathwayreporting systems can be used to assess the in vivo activation of signaltransduction pathways. To expand the utility of these systems, Stratagene isreplacing the pFA-cJun...

Easy Cosmid DNA Purification with StrataPrep Kits

Stratagene plasmid miniprep and midiprep kits aredesigned to isolate up to 20 and 350 g, respectively, of high-purity plasmidDNA from a single spin cup. These kits can also easily purify verylarge plasmids, inc...

New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways

Stratagene cis-reportingsystems now includes the GAS and ISRE reporting systems, to better study theactivation of signal transduction pathways stimulated by int...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

Stratagene cis-reporter plasmid,the pNFAT-Luc reporter plasmid, is designed for studying the activation of thenuclear factor of activated T-cell (NFAT) transcription factors...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

reporting system for studyingNFAT activation Stratagene cis-reporterplasmid, the pNFAT-Luc reporter plasmid, is designed for studying the activationof the nuclear factor of activated T-cell (NFAT) tr...

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf<sup > Plasmid Mini Kit BioSystems Laboratory, Brinkmann Instruments, Inc., We...

Sample Preparation for Microinjection

Microinjection is the loading or transfer of a dissolved substance into a living cell. The microscopic tip of the glass microcapillary has an inner diameter between 0.2 and 1 m [1]. This capillary is back loaded with the sub...

PatchMan - Test Report

The new Ependorf Micromanipulator for electro-physiology, PatchMan, was tested under practical conditions at the Max-Planck-Institute (MPI) for Biophysical Chemistry in Gttingen (Germany). Eppendorf received the following report: The Micromanipulator PatchMan w...

Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit

Gel Cleanup Kit George Halley, Eppendorf 5 Prime, Inc. Agarose gel electrophoresis is used extensively to separate and analyze DNA that is subsequently used for downstream applic...

Eppendorf Perfectprep Gel Cleanup Kit

Gel Cleanup Kit Blunt-end Cloning of DNA Isolated Using the Eppendorf Perfectprep Gel Cleanup Kit Eppendorf 5 Prime, Inc. October 1, 2002 Agarose gel el...

Eppendorf Perfectprep Gel Cleanup Kit

Gel Cleanup Kit Eppendorf 5 Prime, Boulder, Colorado The purity of DNA extracted from agarose gels can have a significant impact on downs...

Genetic Reporter Systems

Genetic reporter systems have developed into an essential tool for examining regulatory promoter and enhancer sequences as well as transcription factors. In most cases, the element under investigation (promoter, enhancer) is cloned together with the reporter gene in an expression vector, which is subsequently used to tra...

Genetic Reporter Systems

Genetic reporter systems have developed into an essential tool for examining regulatory promoter and enhancer sequences as well as transcription factors. In most cases, the element under investigation (promoter, enhancer) is cloned together with the reporter gene in an expression vector, which is subsequently used to tra...

Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit

Plasmid Mini Kit James Griffith, University of Georgia, Department of Genetics, Athens, GA Kimberly Vollmer, Brinkmann Instruments, Biosytems Application Lab, Westbury, NY...

Eppendorf Perfectprep Plasmid DNA Isolation Kits

Plasmid DNA Isolation Kits Eppendorf Perfectprep Plasmid DNA Isolation Kits are designed to generate high yields of highly pure plasmid DNA suitable for automated fluorescent sequencing, transfection, PCR, and other extremely sensitive downstream applications....

Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel

Christian Bauer, Oliver Klotzsche, CCS Cell Culture Service GmbH, Falkenried 88, D-20251 Hamburg, Germany, Frank Apostel, Eppendorf AG, Germany The classic RNA isolation procedures are...

Streptococcus salivarius

Streptococcus salivarius...

Streptococcus salivarius

Streptococcus salivarius...

DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification

QIAGEN introduces the DirectPrep 96 Miniprep System for cost-effective, fully automated, high-throughput DNA purification from high-copy plasmids. Use of a novel lysis chemistry eliminates the need for a lysate clearing step. Plasmid DNA purification is performed using a single plate and a simple bindwashelute...

QIAprep Spin Miniprep Kit

For 124 high-purity plasmid minipreps Spin columns Centrifugation QIAprep spin columns...

Plasmid Miniprep Kit Selection

For high- and low-copy plasmid and cosmid DNA preparations of * Option to automate For use on robotic workstations...

Genomic DNA Preparation from RNAlater Preserved Tissues

The following protocol is suitable for 100 mg tissue samples. Mouse kidney, lung, and spleen genomic DNA have been successfully extracted with approximate yields of 70 g DNA per 100 mg tissue....

RNAPrep - Total RNA Isolation Purification

based magnetic bead purification kit for extracting and purifying total RNA from Eukaryotic cultured cells. RNAPrep is a simple and reproducible 3-step procedure for isolating total RNA for use in applications such as microarray and RT-PCR gene expression analysis. RNAPreps automation friendly...

Separation Reproducibility With the BioLogic Chromatography Systems

Kevin Thornton, PhD, Bio-Rad Laboratories, Inc., Hercules, CA 94547 USA In chromatographic separations, sample component elution times are often used both for identification and to program automated chromatography steps like fraction collection. Therefore, the ability to obtain reproducible...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

Contributed by James V. Anderson and Charles L. Guy, The University of Florida, Institute of Food and Agricultural Sciences BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated Proteins) are ER-luminal proteins and members of the HSP70 family. 1</...

Detection of Platinum Species in Plant Material by Preparative Isotachophoresis Using the Model 491 Prep Cell, Rev A

Contributed by J. Messerschmidt and F. Alt, Institut frSpektrochemie und angewandte Spektroskopie, Bunsen-Kirchhoff-Str. 11, D-44139 Dortmund, Germany Abstract An isotachophoretic method is presented for the separation of platinum containing proteins from plant material in the Model 491 Prep Cell. Platinum-contai...

A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)

β -lactamases are either plasmid or chromosomally encoded bacterial enzymes which hydrolyze β -lactam antibiotics. The majority of β -lactamases produced by clinical isolates are serine active site enzymes. One successful approach to combating the serine active site β -lactamases is the use of β -lactamase inhibit...

Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)

Evelyn McGown, Ph.D. , Michael Su, M.S. and Jin Liao, M.D., Ph.D. Molecular Devices Corporation, 8/00 Luciferase from the North American firefly (Photinus pyralis) is very popular foruse in Reporter Gene Assays. The enzyme catalyzes the ATP-dependent oxidationof luciferin with the concomitant release of light...

Sonic Foundry reports net loss, higher revenue

The quarterly net loss was slightly lower than the $1.6 million or 5 cents per...

Inner-city entrepreneurship isn't 'Easy Street,' but some are walking the walk

At a meeting in the church last week, Alder Michael McGee Jr. wanted to discuss a different type of angel "angel investors." These angels...