Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
Danny Q. Hoang Michael Browning Xinping Yang PeterPingerelli Stratagene Cloning Systems, Inc. Stratagene introduces affinity purified antibodies for NMDA receptorsubunits that are useful for studying expression of the NR2A, NR2B and NR2Csubunit proteins...Counting Colonies or Plaques Directly from Images of Agar Plates
Michelle Cayouette Jane Moores Michael Steege AlanOrr Stratagene Cloning Systems, Inc. software version 3.1,researchers have the ability to automatically count bacterial colonies andplaques on agar plates. Researchers can use...Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
In situ Adapter for the RoboCycler 40 Temperature Cycler allows rapidamplification of rare mRNA Department of Medicine, University of Illinois at Chicago Figure 1: RoboCycler 40 Temperature Cycler In Situ Adapter Syst...High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
QuikChange XL: a highly versatile kit for site-directed mutagenesis oflarge plasmids Michael Borns Peter Vaillancourt Holly Hogrefe Jeff Braman Stratagene The QuikChange site-directed mutagenesis kit* characteristicallygenerates mutagenesis efficiencies exceeding 80%. We have created a new kit formutagenesis of large plasmid...CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
Stephen Roemer Mary Jensen Harry Osterman MarkVanek LI-COR Biotechnology Division LI-COR and Stratagene release the first commercially available precastgels for automated DNA sequencing and genetic analysis. Stratagenes CastAway gels* for...Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
Denise L. Wyborski * John C. Bauer * Barbara McGowan * JosephA. Sorge * Peter Vaillancourt Stratagene Cloning Systems, Inc. Stratagene has improved the Affinity protein expression andpurification system by the addition of the pCAL-n-EK vector. This E. coliclonin...pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
Faster, easier screening for difficult transfections when using thecomplete control kit Stratagene We describe the vector pFB-ERV, an MMLV-based replication-defectiveretroviral vector for delivery of the ecdysone receptor proteins RXR and VgEcR.In stable cell lines infected with an estima...Four New CastAway Precast Gels
CastAway precast gels* eliminate the need to manually pour gels, saving upto 4 hours preparation time. Stratagenes quality guarantee means no more gelslost to bubbles or leaking.All CastAway gels are guaranteed to maintainstability for a minimum of 60 days from the date of manufacture, with nodetectable deterioration in band resolution. 6% CastAway Gels with 32 Preform...Constructing Directional cDNA Libraries from Limited Amounts of RNA
Barry R. Neiditch Cherie Dewar Tanya Hosfield Marie Callahan John C. Bauer Mike Kobrin Quinn Lu Dianne Eckhardt Stratagene Human Genome Sciences, Rockville, Maryland Stratagene has developed the PCR Library Con...Generate High-Quality, Directional Plasmid cDNA Libraries
Produce larger primary libraries with new plasmid library constructionkits Quinn Lu Marie Callahan Tanya Hosfield BarryNeiditch Alan Greener John C. Bauer Mike Kobrin Stratagene II,pcmv-script and pAD-GAL4-2.1 XR library construction kit...Gene-Specific Primers for NMDA Receptor Subunits
Robert Buchner Peter L. Pingerelli Michael D. Browning Mary Ellen Simcox Stratagene Cloning Systems, Inc. Stratagene is now offering gene-specific primer sets for the rat and humanNMDA receptor. In this article, we describe using these primers in reversetran...New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
Carsten-Peter Carstens Julie Bonnardel Anna Waesche Ronda Allen Stratagene Codon bias is a significant obstacle for efficient expression ofheterologous genes in E. coli hosts. In GC-rich genomes, such as mammals,rare arginine codons (AGG or AGA) and the proline codon (CCC) most fre...TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
Institute for Molecular Biology and Biotechnology, McMaster University,Ontario, Canada GST-fusion proteins expressed in TKB1 bacteria are used to affinity purifycellular proteins, which interact with a specific autophosphorylation site onthe Erb...Recovery of DNA from LMP Agarose1
Resolve DNA fragments on a Low Melting Point (LMP) agarose gel in 1x Tris-Acetate-EDTA (TAE) buffer. Tris-Borate-EDTA (TBE) is not recommended as TBE gels are much more difficult to solubilize. Stain gel with Ethidium...Efficient Recovery of Ultrapure Plasmid DNA
Nicole Passaretti, Vincent Prezioso, PhDBrinkmann Instruments, Inc., Westbury, NY Susanne WagnerEppendorfNethelerHinz GmbH, Hamburg, Germany Plasmids are naturally occurring, extrachromosomal DNA...TripleMaster PCR System: Direct Comparison with Competitors
Eppendorf 5 Prime, Inc., Boulder, CO, USA This experiment was performed to compare the Eppendorf TripleMaster PCR System with the long-range PCR products of leading competitors. Eppendorf was compared to competitors T...DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
QIAGEN introduces the DirectPrep 96 Miniprep System for cost-effective, fully automated, high-throughput DNA purification from high-copy plasmids. Use of a novel lysis chemistry eliminates the need for a lysate clearing step. Plasmid DNA purification is performed using a single plate and a simple bindwashelute...Purification of Recombinant Flavonone 3beta-Hydroxylase Using Bio-Scale CHT5-1 Hydroxyapatite Column
Flavanone 3b-hydroxylase (FHT), a typical 2-oxoglutaratedependent dioxygenase, catalyzes the 3b-hydroxylation of 2S-flavanones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavanoids, catechins, proanthocyanidins, and anthocyanidins in plants. To understand the complex reaction mechanism,...Receptor Binding Assays using Fluorescence Polarization Detection: the [FP]2TM Characterized Tracers
. The process is typically highly automated and can provide screening rates over 100,000 samples in a 24 hour period. To achieve this, assays must be simple, p...Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.
Recent advances in combinatorial chemistry and genomics programs have led to an unprecedented increase in both the number of compounds and potential drug targets that can be screened. The development of automated liquid h...Gq-coupled Receptor Assays Using FLEXstation
Departments of...Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor
SIMPLE, RAPID EXPERIMENTAL SET-UP AND DATA ANALYSIS DETERMINE EC EXCELLENT FOR ASSAY DEVELOP-MENT AS DEMONSTRATED BY Z FACTOR INTROD...Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
SIMPLE, FAST EXPERIMENTAL SET-UP Activation of G -coupled...Sciclone ALH Bulk Dispense Precision Testing
Caliper Life Sciences Inc., Applications Laboratory, Hopkinton, Massachusetts, USA The ability to continuously dispense reagents across multi-well plates and into individual columns (secondary dispensing) is of great importance to high throughput laboratories. Equally important,...96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation
Initially, work in 96 well microplat...96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
The purpose of this...Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
Caliper Life Sciences, Inc., Applications Laboratory, Hopkinton, Massachusetts, USA The Z8 liquid handling device is an optional accessory to the Sciclone Advanced Liquid Handler (ALH). This device is designed to permit individually and independently controlled access to single we...Doyle seeks $30M for e-medical record adoption
But will that level of commitment be enough? Doyle, speaking at the east clinic of <a href="http://www.deancare....Real pulse of the economy? Check real estate foreclosures
annual forecast for this year, I thought it would be a good idea to go back and look at the foreclosures on houses. You would think that by now the traction of the economy would grip and people would start to be more financially secure, but it does not look tha...Medical College researchers receive new grants to enhance discovery of heart-protecting anesthetics
Zeljko Bosnjak, Ph.D., professor and vi...Platypus Technologies receives $1.6 million NIH grant
The Small Business Innovation Research grant is for continued development of the company's proprietary liquid crystal based technology to enable faster screening of drugs for treatment of cancer. The com...