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Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

Efficient gene transfer and high-level protein expression within a varietyof cells Stratagene Recombinant adenoviruses provide a versatile means of gene delivery andexpression. We describe the AdEasy adenoviral vectorsystem, whichsimp...

Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling

Danny Q. Hoang Michael Browning Xinping Yang PeterPingerelli Stratagene Cloning Systems, Inc. Stratagene introduces affinity purified antibodies for NMDA receptorsubunits that are useful for studying expression of the NR2A, NR2B and NR2Csubunit proteins...

Counting Colonies or Plaques Directly from Images of Agar Plates

Michelle Cayouette Jane Moores Michael Steege AlanOrr Stratagene Cloning Systems, Inc. software version 3.1,researchers have the ability to automatically count bacterial colonies andplaques on agar plates. Researchers can use...

Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue

In situ Adapter for the RoboCycler 40 Temperature Cycler allows rapidamplification of rare mRNA Department of Medicine, University of Illinois at Chicago Figure 1: RoboCycler 40 Temperature Cycler In Situ Adapter Syst...

High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors

QuikChange XL: a highly versatile kit for site-directed mutagenesis oflarge plasmids Michael Borns Peter Vaillancourt Holly Hogrefe Jeff Braman Stratagene The QuikChange site-directed mutagenesis kit* characteristicallygenerates mutagenesis efficiencies exceeding 80%. We have created a new kit formutagenesis of large plasmid...

CastAway Precast Gels for Rapid Automated DNA Sequence Analysis

Stephen Roemer Mary Jensen Harry Osterman MarkVanek LI-COR Biotechnology Division LI-COR and Stratagene release the first commercially available precastgels for automated DNA sequencing and genetic analysis. Stratagenes CastAway gels* for...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

Denise L. Wyborski * John C. Bauer * Barbara McGowan * JosephA. Sorge * Peter Vaillancourt Stratagene Cloning Systems, Inc. Stratagene has improved the Affinity protein expression andpurification system by the addition of the pCAL-n-EK vector. This E. coliclonin...

pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins

Faster, easier screening for difficult transfections when using thecomplete control kit Stratagene We describe the vector pFB-ERV, an MMLV-based replication-defectiveretroviral vector for delivery of the ecdysone receptor proteins RXR and VgEcR.In stable cell lines infected with an estima...

Four New CastAway Precast Gels

CastAway precast gels* eliminate the need to manually pour gels, saving upto 4 hours preparation time. Stratagenes quality guarantee means no more gelslost to bubbles or leaking.All CastAway gels are guaranteed to maintainstability for a minimum of 60 days from the date of manufacture, with nodetectable deterioration in band resolution. 6% CastAway Gels with 32 Preform...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

Barry R. Neiditch Cherie Dewar Tanya Hosfield Marie Callahan John C. Bauer Mike Kobrin Quinn Lu Dianne Eckhardt Stratagene Human Genome Sciences, Rockville, Maryland Stratagene has developed the PCR Library Con...

Generate High-Quality, Directional Plasmid cDNA Libraries

Produce larger primary libraries with new plasmid library constructionkits Quinn Lu Marie Callahan Tanya Hosfield BarryNeiditch Alan Greener John C. Bauer Mike Kobrin Stratagene II,pcmv-script and pAD-GAL4-2.1 XR library construction kit...

Gene-Specific Primers for NMDA Receptor Subunits

Robert Buchner Peter L. Pingerelli Michael D. Browning Mary Ellen Simcox Stratagene Cloning Systems, Inc. Stratagene is now offering gene-specific primer sets for the rat and humanNMDA receptor. In this article, we describe using these primers in reversetran...

New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes

Carsten-Peter Carstens Julie Bonnardel Anna Waesche Ronda Allen Stratagene Codon bias is a significant obstacle for efficient expression ofheterologous genes in E. coli hosts. In GC-rich genomes, such as mammals,rare arginine codons (AGG or AGA) and the proline codon (CCC) most fre...

TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins

Institute for Molecular Biology and Biotechnology, McMaster University,Ontario, Canada GST-fusion proteins expressed in TKB1 bacteria are used to affinity purifycellular proteins, which interact with a specific autophosphorylation site onthe Erb...

Recovery of DNA from LMP Agarose1

Resolve DNA fragments on a Low Melting Point (LMP) agarose gel in 1x Tris-Acetate-EDTA (TAE) buffer. Tris-Borate-EDTA (TBE) is not recommended as TBE gels are much more difficult to solubilize. Stain gel with Ethidium...

Efficient Recovery of Ultrapure Plasmid DNA

Nicole Passaretti, Vincent Prezioso, PhDBrinkmann Instruments, Inc., Westbury, NY Susanne WagnerEppendorfNethelerHinz GmbH, Hamburg, Germany Plasmids are naturally occurring, extrachromosomal DNA...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In man...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In many research projects, cD...

TripleMaster PCR System: Direct Comparison with Competitors

Eppendorf 5 Prime, Inc., Boulder, CO, USA This experiment was performed to compare the Eppendorf TripleMaster PCR System with the long-range PCR products of leading competitors. Eppendorf was compared to competitors T...

DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification

QIAGEN introduces the DirectPrep 96 Miniprep System for cost-effective, fully automated, high-throughput DNA purification from high-copy plasmids. Use of a novel lysis chemistry eliminates the need for a lysate clearing step. Plasmid DNA purification is performed using a single plate and a simple bindwashelute...

Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer

David Barnidge , Gargi Choudhary Neuromics, Inc., Minneapolis, MN Thermo Finnigan, San Jose, CA The data presented here was acquired using the Proteome...

Purification of Recombinant Flavonone 3beta-Hydroxylase Using Bio-Scale CHT5-1 Hydroxyapatite Column

Flavanone 3b-hydroxylase (FHT), a typical 2-oxoglutaratedependent dioxygenase, catalyzes the 3b-hydroxylation of 2S-flavanones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavanoids, catechins, proanthocyanidins, and anthocyanidins in plants. To understand the complex reaction mechanism,...

Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A

Joseph W. Turek and Guy C. Le Breton, Department of Pharmacology,University of Illinois at Chicago, College of Medicine, Chicago, IL 60612.Address correspondence to: Joseph W. Turek, 835 South Wolcott, Departmentof Pharmacology M/C 868, University of Illinois at Chicago, Chicago, IL 60612.E-mail: jturek1@icarus.uic.edu Introduction...

Receptor Binding Assays using Fluorescence Polarization Detection: the [FP]2TM Characterized Tracers

. The process is typically highly automated and can provide screening rates over 100,000 samples in a 24 hour period. To achieve this, assays must be simple, p...

Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.

Recent advances in combinatorial chemistry and genomics programs have led to an unprecedented increase in both the number of compounds and potential drug targets that can be screened. The development of automated liquid h...

Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)

Jinfang Liao, M.D., Ph.D. and Evelyn McGown, Ph.D. Molecular Devices Corporation, 8/00 The photoprotein aequorin, a bioluminescent protein from the coelentratejellyfish Aequorea victorea, has proven to be highly sensitive to the trace amount of Ca in cells. The aequorin complex which contains the...

Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)

Evelyn McGown, Ph.D. , Michael Su, M.S. and Jin Liao, M.D., Ph.D. Molecular Devices Corporation, 8/00 Luciferase from the North American firefly (Photinus pyralis) is very popular foruse in Reporter Gene Assays. The enzyme catalyzes the ATP-dependent oxidationof luciferin with the concomitant release of light...

Gq-coupled Receptor Assays Using FLEXstation

Departments of...

Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor

SIMPLE, RAPID EXPERIMENTAL SET-UP AND DATA ANALYSIS DETERMINE EC EXCELLENT FOR ASSAY DEVELOP-MENT AS DEMONSTRATED BY Z FACTOR INTROD...

Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

Simon Lydford & Petra Averbeck, Molecular Devices Limited, U.K. The FlexStation scanning fluorometer integrates sensitive optics, fluid transfer and temperature control, making it ideal for kinetic, cell-based fluorometric assays, such as the measurement of intracellular calcium, membrane potential and intracellular pH. <...

Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR

SIMPLE, FAST EXPERIMENTAL SET-UP Activation of G -coupled...

Sciclone ALH Bulk Dispense Precision Testing

Caliper Life Sciences Inc., Applications Laboratory, Hopkinton, Massachusetts, USA The ability to continuously dispense reagents across multi-well plates and into individual columns (secondary dispensing) is of great importance to high throughput laboratories. Equally important,...

96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation

Initially, work in 96 well microplat...

96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array

The purpose of this...

Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing

Applied Science and Technology Department, Caliper Life Sciences, Inc., Hopkinton, MA 01748 The High Volume Head is one of four multi-channel liquid handling options available for the Sciclone Advanced Liquid Handler (ALH). The architecture of the device includes an arra...

Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option

Caliper Life Sciences, Inc., Applications Laboratory, Hopkinton, Massachusetts, USA The Z8 liquid handling device is an optional accessory to the Sciclone Advanced Liquid Handler (ALH). This device is designed to permit individually and independently controlled access to single we...

Doyle seeks $30M for e-medical record adoption

But will that level of commitment be enough? Doyle, speaking at the east clinic of <a href="http://www.deancare....

Real pulse of the economy? Check real estate foreclosures

annual forecast for this year, I thought it would be a good idea to go back and look at the foreclosures on houses. You would think that by now the traction of the economy would grip and people would start to be more financially secure, but it does not look tha...

Medical College researchers receive new grants to enhance discovery of heart-protecting anesthetics

Zeljko Bosnjak, Ph.D., professor and vi...

Platypus Technologies receives $1.6 million NIH grant

The Small Business Innovation Research grant is for continued development of the company's proprietary liquid crystal based technology to enable faster screening of drugs for treatment of cancer. The com...