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Quantitation in Biological Technology

ForteBio Launches New Assay Capabilities for High-Sensitivity Protein Quantitation and Fragment Screening on Its Octet(R) Platform

MENLO PARK, Calif., May 27 /PRNewswire/ -- ForteBio(R), Inc., a leading supplier of label-free technology that accelerates the development of biotherapeutic and pharmaceutical products, today announced that it has launched two new assay modules - for high-sensitivity protein quantitation and fragm...

Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

Gargi Choudhary, Tania A. Sasaki, and Julian J. Phillips, Thermo Electron Corporation, San Jose, CA Chromatography and Mass Spectrometry Application Report Introduction Ion trap mass spectrometry is widely recognized for solving analytical problems requi...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 INTRODUCTION Reporter gene assays are used to study eukaryotic gene expression. Dual geneticreporters are commonly used in transient transfections of cultured cells tominimiz...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 INTRODUCTION DNA measurements are important for many aspects of molecular biology. Of thespectrophotometric methods available, UV absorbance measurement at 260 nm isthe simpl...

ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 INTRODUCTION Adenosine triphosphate (ATP) is present in all living cells. Because the level isstrictly-controlled, assay of ATP can be used as a indicator of viable cell numb...

Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

By Yan Zhang., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. Introduction This application note describes how to use the OliGreen Quantitation Kit from Molecular Probes in the Gemini XS, Gemini EM and SpectraMax M2 mic...

Achieving Reliable Protein Quantitation Results Using ICAT Reagents, MALDITOF Mass Spectrometry, and Advanced Data Interpretation Tool

Overview A challenge of quantitative analysis of complex protein mixtures using isotope coded affinity tags is to correctly identify and obtain accurate quantitative information for ICAT reagent pairs on a spectrum generated by mass spectrometry. Because MALDI-TOF mass spectr...

Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry

Overview Traditionally, MALDI-TOF mass spectrometry has been used very little for quantitative studies. Analysts have usually assumed that potential differences in ionization efficiency between analyte and internal standards and an inherent heterogeneity of the sample spot wo...

Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System

Purpose Normal phase chromatography can be used for the analysis of chiral compounds using the API 3000 LC/MS/MS System. Overview In recent years, pharmaceutical companies have placed greater importance on examining the stereoisomeric composition of dru...

A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)

Purpose A previously published application note in this series describes an LC/MS/MS method for the simultaneous determination of four immunosuppressant drugs: Cyclosporin A (CsA), Tacrolimus (TAC, aka FK-506), Sirolimus (SIR, aka Rapamycin) and Everolimus (EVE, aka RAD-001) ...

A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders

Purpose The peroxisome is a cellular organelle that plays an important role in a number of functions, including the beta-oxidation of very long chain fatty acids (VLCFA). Peroxisomal disorders are characterized by impaired, reduced or total absence of peroxisomes in cells, wh...

High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS

A sensitive and simple high performance liquid chromatograph tandem mass spectrometry (LC/MS/MS) method is presented for a simultaneous analysis of the metabolites of four nitrofuran antibiotics in chicken tissue. Abstract The animal meat samples were prepared by ho...

Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting

O. Rönn 1 , M. Landström 2 , A. Edman-Örlefors 1 , and S. Edlund 1 1 GE Healthcare, Uppsala, Sweden 2 Ludwig Institute for Cancer Research, Uppsala, Sweden ECL Plex™ Western Blotting Detection System ...

Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

Key words: multidimensional liquid chromatography (MDLC) • reversed-phase chromatography (RPC) • LC-MS/MS • DeCyder MS • differential analysis Ettan™ MDLC was used in conjunction with tandem mass spectrometry (MS/MS)...

Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A

Theresa Redila-Flores, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA The Bradford and Lowry assays are 2 colorimetric assays that are commonly used to quantitate protein concentration. Bio-Rads protein assay (catalog ...

Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer

Mark Churchill and Mark Harrison, Thermo Finnigan, Hemel Hempstead, UK Bioanalytical Method Validation The data presented here can be acquired using a Thermo Finniga...

Comparison of Different Methods of mRNA Quantitation

Feature Nuclease Protection Assays Northern Blotting RT-PCR ...

CapitalBio Corporation and VigeneTech Inc. Have Announced the Launch of a Fully Integrated Microarray Solution Platform

...e analysis, which delivers unprecedented accuracy and reproducibility; and VigeneTech's CellVigene(TM), which provides superior signal extraction and quantitation of cell/tissue-based images for diagnostic and pre-clinical high-content applications. For more information, visit http://www.vigenetech.com . ...

Supelco(R) and MIP Technologies Launch New SPE Product for the Selective Extraction of NSAIDs From Environmental Water

...implify the analysis of these drugs when present, at even low levels, in natural water samples." SupelMIP was developed for confirmation and quantitation of NSAID drugs in water samples. The SPE phase has been developed for multi-residue extraction of NSAIDs and has been validated for ibuprofen, diclofe...

ForteBio Launches Two Revolutionary High-Throughput, Label-Free Instruments: The Octet(R) RED384 and the Octet QK384

...er time-to-result for kinetic characterization and quantitation measurements. "The Octet RED384 and Octet QK3...g cost in the 10-20 cents range per data point for quantitation and kinetic characterization assays. The new ...tandard microplate format. Its biosensors simplify quantitation and kinetic characterization by eliminating throug...

PharmaNet Development Group Clinical Research Experts to Present at Global Professional Meetings in June

...ssociate Director, Immunochemistry, will also present their poster entitled "Optimization of Commercial ELISA Kits for the quantitation of Human Luteinizing Hormone and Follicle Stimulating Hormone." June 30 4th Annual Biopharm Asia Conference Singapo...

ForteBio, Inc. Closes $25 Million In Series C Financing

...io introduced its Octet RED system and additional biosensors, expanding its quantitation applications and broadening its small molecule kinetics capabilities. The c...celerate life science and pharmaceutical development. These systems provide quantitation and kinetic information, enabling real-time analysis of biomolecular intera...

USP Announces Revised Glycerin Monograph

...edure, Diethylene glycol and ethylene glycol impurities, capable of quantifying diethylene glycol and ethylene glycol at or above the Limit of quantitation (0.025%) is added. This diethylene glycol and ethylene glycol impurities test will be added to ascertain the level at which these impurities a...

Rosetta Genomics Selects PCR as Platform for its microRNA-based Diagnostics to be Launched in 2008, Licensed Nanogen's MGB Probe Technology

...omics has developed a panel of microRNA biomarkers potentially able to identify approximately thirty cancers "Our proprietary microRNA detection and quantitation technologies are at the heart of our sensitive diagnostic products," noted Amir Avniel, President and CEO of Rosetta Genomics." Using very small amoun...

Bruker Announces Availability of New HUPO World Congress Proteomics Posters on Web Site

...or-Controlled Preparation Technique for MALDI Tissue Imaging Poster M102 -- Towards Stable Diagnostic Setups in Clinical Proteomics: Absolute quantitation of Peptide Biomarkers Using Immuno-Capturing and MALDI-TOF MS Poster M74 -- Standardization of MALDI-TOF mass spectrometry based proteomic pr...

Protein Depletion for Plasma and Serum Proteomic Analysis

...eline profile includes both the identification and quantitation of different proteins. Such a baseline ...ow pg/ml levels. Based on this wide dynamic range, quantitation of all proteins simultaneously in a sin...ical procedures of identification and quantitation of post-translational chemically modified amino ac...

Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

...plication Report Key Words Sensitivity quantitation Finnigan LCQ Deca XP Plus Equine serum Aceproma...um. Determine limits of detection (LOD) and quantitation (LOQ) of these two compounds in serum. Demo...shown in Table 1. quantitation and Linear Dynamic Range Figures 2 and 3 show cal...

Trace Analysis Optimizing Sensitivity Finnigan Surveyor PDA Detector

... HPLC is widely used in the analysis of trace components, i.e. quantitation of trace components is regularly performed by measurement of peak height, t...will be seen which will allow better accuracy and precision at the limit of quantitation (LOQ). Noise is made up of two components: short-term and long-term noise. ...

Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery

...Application Note Key Words Sensitivity quantitation Finnigan TSQ Quantum Discovery Enhanced Mass- Re... Overview Comparison of the limit of quantitation (LOQ) of a drug discovery compound in plasma at un...niques and instrumentation are revolutionizing the quantitation of pharmaceutical products. [1] The benchtop Finn...

Quantitation of Acrylamide in Food Samples on the Finnigan TSQ Quantum Discovery by LC/APCI-MS/MS

...amide signal observed for the m/z 72→55 SRM transition at the same retention time as the 50 ng/mL d3-acrylamide standard. The limit of quantitation (LOQ) for acrylamide on the Finnigan TSQ Quantum Discovery was 0.25 ng/mL acrylamide or 2.5 pg on column (Figure 2). This compares favorably to LOQs p...

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

...mersham BioSciences P/N 25-6400-01) DYEnamic ET Terminator Sequencing Kit (Amersham BioSciences P/N US81095) PicoGreen dsDNA quantitation Kit (Molecular Probes P/N P-7589) Deck Layout: For the TempliPhi Kit, the deck layout is shown in Figure 1. Position A1 is a ti...

Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)

...ositive SD of the blank) is approximately 2 fg/well, and the lower limit of quantitation (based on amount giving a signal 10 positive SD of the blank) is approximat...e Assay Kit makes the measurements easy and sensitive with a lower limit of quantitation for (recombinant enzyme) of approximately 6 fg/well. For measuring lucifera...

Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)

...note describes how to use the NanoOrange Protein quantitation Kit from Molecular Probes in the Gemini XS, Gemini... MATERIALS NanoOrange Protein quantitation Kit from Molecular Probes (catalog number (N-6666)...e the concentrated Component B (NanoOrange protein quantitation diluent) 10x in distilled water (one part Componen...

QBT Fatty Acid Uptake Assay Kit datasheet

... CONVENTIONAL METHODS The development of fatty acid uptake regulators as potential drugs for metabolic diseases requires robust assays for the quantitation of fatty acid uptake. Conventional protocols utilizing radioactivity often require cell lysis and processing at very low temperature, making them expe...

Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

...y be used. With small sample loads, counting efficiencies are comparable to conventional LSC. For assays prepared in the microplate, TopCount provides quantitation in the same format. This results in increased throughput, less sample handling, and significant savings in reagent and waste disposal costs. ...

New Tools for Making and Processing Protein Microarrays.

...for their contributions and comments in writing this article. References 1. M. N. Bobrow and G.J. Litt: Method for detection or quantitation of an analyte using an analyte dependent enzyme activation system. US Patent 5,196,306. 2. M. N. Bobrow and G.J. Litt: Catalyzed Reporter D...

Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter

...s. The phosphorylation of proteins by kinases is an important cellular control mechanism. In addition, labeling of PCR products with 32 P allows easy quantitation of PCR reactions. All of these applications are easily done in a microplate or a microplate format dot blot apparatus. The sample is measured directly...

Amplification Efficiency of TaqMan Gene Expression Assays

...show that the ΔΔC T method for relative quantitation is better correlated with expected fold change in ... to use the ΔΔC T method for relative quantitation as outlined in Applied Biosystems User Bulletin #2...4% agarose gel (NuSieve / SeaKem ) with size and quantitation standards (25 bp ladder from Invitrogen) to estima...

Identification of Phase I and Phase II Metabolites of Buspirone on the Q TRAP LC/MS/MS System

...cans, can be used in flexible combinations to achieve unprecedented selectivity Broad linear dynamic range provides true triple quadrupole quantitation performance and enhances identification of ions in complex matrices Information-rich MS/MS and MS3 spectra with important low mass structu...

Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method

...these metabolites was determined to be: 0.5, 0.25 and 0.25 pg (4-hydroxytolbutamide, -hydroxymidazolam and dextrorphan, respectively) and the limit of quantitation for each of these metabolites was determined to be: 1.0, 0.5 and 0.5 pg, respectively. Calibration curves for the three metabolites were run...
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