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Purification in Biological Technology

SANYO Donates Air Purification Systems Effective Against Flu Viruses to New York Airports and Schools

Some Recipients have recently faced H1N1 Flu Outbreaks JAMAICA, N.Y., May 26 /PRNewswire/ -- SANYO Biomedical and Environmental Solutions (SANYO), a division of SANYO North America, announces the donation of professional-use air purification systems to provide an added level of safe, healthy ...

Water purification down the nanotubes

Nanotechnology could be the answer to ensuring a safe supply of drinking water for regions of the world stricken by periodic drought or where water contamination is rife. Writing in the International Journal of Nuclear Desalination, researchers in India explain how carbon nanotubes could replace...

Norgen Biotek Corp. Launches Four New Kits for the Purification of RNA and DNA from Plants and Fungi

THOROLD, ON, July 18 /PRNewswire/ - Norgen Biotek Corp., an innovative Canadian biotechnology company focusing primarily on advancing powerful tools for RNA, DNA and protein purifications, today announced the expansion of its product offerings by launching four new kits geared towards the isol...

Semi Preparative Purification of Proteins

Most Basic Recommended Cecil Instrumentation: Cecil Adept HPLC System 4BP, semi-preparative, biocompatible with a PEEK 500 l loop Fraction collector Column heater Introduction : Researchers need to understand the expression of prote...

DNA purification from pig ears to bird blood using proven DNeasy Technology

DNeasy Blood & Tissue Kits provide fast and easy silica-based DNA purification in convenient spin-column and 96-well-plate formats. Most samples can be directly lysed with proteinase K, eliminating the need for mechanical disruption and reducing hands-on time. Optimized pr...

Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

Abstract Applied Biosystems has developed new techniques that produce highpurity total RNA from biological sources such as cultured cells. These techniques eliminate the need for post-isolation DNase treatment or other manual handling, and produce a highly pure product, free ...

New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

A. Bergh, M. Carlsson, A. Karlsson, and H. Lindgren GE Healthcare, Uppsala, Sweden Histidine-tagged proteins* can be quickly and simply purified on His GraviTrap columns prepacked with Ni Sepharose 6 Fast Flow without the need for a pump o...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

Contributed by James V. Anderson and Charles L. Guy, The University of Florida, Institute of Food and Agricultural Sciences Introduction BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated Proteins)...

Enzyme Purification With the Econo-Pac Q Cartridge

Data submitted by Dr. Ivan Kaiser, University of Wyoming, Laramie, WY USA Summary Dr. Ivan Kaisers research involves the isolation and purification of proteins from snake venom, such as post-synaptic neurotoxins and hemorrhagic ...

Whole Gel Eluter Purification of a Functional Multiprotein DNA Replication Complex, Rev A

HaiYan Jiang a,e , Robert J. Hickey a-e, h , Pamela E. Bechtel f , Philip W. Wills a, e , SuHua Han a,e , Timothy D. Tom a-g , YueTong Wei a,e , and Linda H. Malkas a-e,+ , (a) Department of Pharmacology and Experimental Therapeutics (b) Program in M...

Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A

Jolanta Kolodziejek, MSc, and Professor Norbert Nowotny, PhD, Clinical Virology Group, Institute of Virology, University of Veterinary Medicine, Vienna , Veterinaerplatz 1, A 1210 Vienna, Austria Introduction The purity of t...

Preparative Nondenaturing Gel Electrophoresis Used in the Purification of an Esterase Involved in Insecticide Resistance, Rev C

Dr Albert J Ketterman and SHP Parakrama Karunaratne, Dept. of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT UK Introduction In attempts to control Culex quinquefasciatus as a worldwide vector...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

K. Sestak, L. Kim, K.-O. Chang and L. J. Saif, Food Animal Health Research Program, Ohio Agricultural Research and Development Program, The Ohio State University, Wooster, OH 44691 Summary Three major structural proteins of the porcine...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

Contributed by James V. Anderson and Charles L. Guy, The University of Florida, Institute of Food and Agricultural Sciences Introduction BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated ...

Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins

Fang-Fang Wu, Xuemei He, and David R Nau, Bio-Rad Laboratories, Inc., Hercules, CA 94547 USA Introduction Immobilized metal affinity chromatography (IMAC) is a powerful technique that can be used for the efficient purification ...

RNA & cDNA Purification Using RNAClean

RNAClean is a SPRI -based nucleic acid purification system that efficiently isolates RNA or cDNA from in vitro transcription, cDNA or RNA labeling and antisense RNA amplification reactions. As gene expression profiling analysis and the use of ...

Ultra-Fast Purification of Small Nucleic Acids

flashPAGE Fractionator System The flashPAGE Fractionator System is the fastest, easiest, and most consistent method for PAGE purification of small nucleic acids. Unlike traditional Polyacrylamide Gel Electrophoresis (PAGE), which ...

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

Lucia Martinez and Ario de Marco EMBL Protein Expression Core Facility, Meyerhofstr. 1, D-69117, Heidelberg, Germany Introduction Immobilized Metal ion Affinity Chromatography (IMAC) is an easy and reliable technique that allows the fast separation of His-tagged proteins ...

Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)

As the human genome sequence project is completed, the vast amount of genomic information calls for methods that allow gene expression analysis on a genomic scale and speed up detection of significant sequence variation...

AMPURE PURIFICATION SYSTEM

AMPure is a high recovery, SPRI-based PCR clean up system capable of purifying amplicons as small as 100bp. AMPure yields high quality PCR amplicons using a simple, three-step protocol requiring no centrifugation ...

QIAquick PCR Purification Kit

For cleanup of DNA from PCR or other enzymatic reactions Application: PCR cleanup Format: Spin columns ...

MinElute PCR Purification Kit

For purification of PCR products in low elution volumes Application: PCR product cleanup Format: Spin columns ...

Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins

Ni-NTA Superflow Columns enable up to 24 automated large-scale 6xHis-tagged protein preparations to be performed in parallel on the BioRobot 3000 workstation. Using cleared lysates from bacterial cultures as starting material, ...

Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation

Stefanie Schrer, Werner Wittke,* and Christian Kupfer QIAGEN GmbH, Hilden, Germany * Leica Microsystems, Wetzlar, Germany Laser microdissection (LMD) provides a powerful t...

Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci

Marion Schmidt, Holger Engel, Markus Sprenger-Hauels, Corinna Kppers, Gaby Schulte, Silke Zobel, and Dirk Lffert QIAGEN GmbH, Hilden, Germany Microsatellites highly polymorphic DNA markers comprised of nucleotides ...

Easy Cosmid DNA Purification with StrataPrep Kits

Miniprep and midiprep quantities of pure high-kb DNA Jeff Braman Scott Basehore Stratagene The StrataPrep plasmid miniprep and midiprep kits are designed to isolate up to 20 and 350 g, respectively, of high-purity plasmid DNA from a single spin cup. 1,2 These kits can a...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

One column purification of protein with native amino acid sequence Denise L. Wyborski * John C. Bauer * Barbara McGowan * Joseph A. Sorge * Peter Vaillancourt Stratagene Cloning Systems, Inc. Stratagene has improved the Affinity protein expression and purification system by t...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acid purification kits Jeff Braman Scott Basehore Stratagene Cloning Systems, Inc. Stratagene announces a new line of DNA purification products, which provide reliable isolation of high-purity miniprep plasmid,...

RNAPrep - Total RNA Isolation Purification

RNAPrep is a SPRI based magnetic bead purification kit for extracting and purifying total RNA from Eukaryotic cultured cells. RNAPrep is a simple and reproducible 3-step procedure for isolating total RNA for use in applications such as microarray...

RNAClean - RNA & cDNA in vitro Reaction Purification

Agencourts RNAClean kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic RT and IVT reactions such as cDNA and cRNA. The kit is formatted for microarray gene expression analysis...

SPRINTPREP SINGLE STEP PLASMID PURIFICATION

SprintPrep is an efficient SPRI-based plasmid purification system that can be completed in less than 15 minutes. Unlike most traditional plasmid purification chemistries, SprintPrep isolates plasmid DNA directly from overnight cu...

COSMCPREP HIGH AND LOW COPY PLASMID PURIFICATION

CosMCPrep is a versatile SPRI-based, plasmid purification system that uses a single protocol to purify a variety of high and low copy number template types. This flexible system is easily automated and produces high quality templ...

Walkaway automation for nucleic acid purification

GenoVision AS, an innovative biotechnology company offering complete walkaway automation for the isolation and purification of nucleic acids, is now part of the QIAGEN Group of Companies and is now known as QIAGEN AS. QIAGEN AS fo...

DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification

QIAGEN introduces the DirectPrep 96 Miniprep System for cost-effective, fully automated, high-throughput DNA purification from high-copy plasmids. Use of a novel lysis chemistry eliminates the need for a lysate clearing step. P...

Rapid heating prepares energy-saving zeolite for greater role in industrial separations

... a variety of applications, including the creation of pure streams of oxygen and other gases, the catalytic cracking of petroleum into gasoline, water purification and softening, the dewatering of ethanol, and as additives in laundry detergents. Zeolite membranes are commonly formed by depositing zeolite cryst...

Nanotech particles affect brain development in mice

...les are increasing worldwide. Titanium dioxide in its nanoparticle form has a high level of photocatalytic activity, and can be used for air and water purification and self-cleaning surfaces. Our findings, however, add to the current concern that this specific nanomaterial may have the potential to affect human h...

PacificGMP and USC to Collaborate on Recombinant Protein Development and Manufacturing for Phase 1 Clinical Trial

...s with drug developers by providing a range of cGMP and non-GMP services from cell line optimization, cell banking, early process design, development, purification and scale-up, to preclinical and clinical manufacturing, aseptic filling, testing, analytical services and regulatory support. More information can be...

Cellular Dynamics International In-Licenses Key Patent Portfolio for Using Stem Cell-Derived Cells in Drug Testing

...ity Indianapolis (IUPUI), covers two important concepts for using stem cell-derived cells for drug screening. First, the Field patents cover a cell purification strategy that enables greater than 90 percent purity of any type of cells. Secondly, the Field patents cover the use of stem cell-derived cardiomyocyt...

Researchers enlist DNA to bring carbon nanotubes' promise closer to reality

...ze individual types, or species, of carbon nanotubes and purify them from a mixture. The new method utilizes tailored DNA sequences and "allows the purification of all 12 major single-chirality semiconducting species from a synthetic mixture, with sufficient yield for both fundamental studies and application d...

Biocept, Inc. Announces Two New Director Appointments

...ines sophisticated attachment chemistry with mathematically modeled fluid dynamics to enable earlier, accurate and less invasive diagnoses. Isolation, purification and genetic analysis of rare cells, such as circulating tumor cells, are managed within our state-of-the-art CLIA-accredited laboratory. The clinical ...
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