Purif

Purified Single-Stranded M13 DNA for Automated Sequencing

A streamlined and reliable method to obtain high-quality single-strandedDNA Stratagene M13 DNA purification kit allowseasy, quick, and reliable purification of bacteriophage single-stranded DNA. Theyield of single-stranded DNA recovered from phage lysa...

A Simple and Rapid Technique to Purify High-Quality Midiprep DNA

Stratagene A new technique is now available to quickly, easily, and reliably isolatehigh-purity plasmid DNA. The convenient spin-format eliminates the need forresin suspensions, gravity flow columns, and alcohol precipit...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

Epitope Tagging in E. coli Katherine Felts Denise Wyborski John Bauer PeterVaillancourt Stratagene LIC cloning kit...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acidpurification kits Stratagene Cloning Systems, Inc. Stratagene announces a new line of DNA purification products, whichprovide reliable isolation of high-purity miniprep plasmid, PCR product andgel-fractionate...

Yeast Protein Production System Features High Yields and One-Step Purification

The ESP system provides high-yield protein production in a eukaryoticorganism Quinn Lu * Bruce Jerpseth * Tim Sanchez * John C. Bauer * AlanGreener Stratagene Cloning Systems, Inc. provides novel opportunities for rapid, inexpensive and high-yield p...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

Denise L. Wyborski * John C. Bauer * Barbara McGowan * JosephA. Sorge * Peter Vaillancourt Stratagene Cloning Systems, Inc. Stratagene has improved the Affinity protein expression andpurification system by the addition of the pCAL-n-EK vector. This E. coliclonin...

Easy Cosmid DNA Purification with StrataPrep Kits

Stratagene plasmid miniprep and midiprep kits aredesigned to isolate up to 20 and 350 g, respectively, of high-purity plasmidDNA from a single spin cup. These kits can also easily purify verylarge plasmids, inc...

Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci

Marion Schmidt, Holger Engel, Markus Sprenger-Hauels, Corinna Kppers, Gaby Schulte, Silke Zobel, and Dirk Lffert QIAGEN GmbH, Hilden, Germany Microsatellites highly polymorphic DNA markers comprised of nucleotides that are repeated in tandem arrays and distributed throughout the geno...

Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation

Stefanie Schrer, Werner Wittke,* and Christian Kupfer QIAGEN GmbH, Hilden, Germany * Leica Microsystems, Wetzlar, Germany Laser microdissection (LMD) provides a powerful technology to isolate specific cells or cell groups for further analysis....

DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification

QIAGEN introduces the DirectPrep 96 Miniprep System for cost-effective, fully automated, high-throughput DNA purification from high-copy plasmids. Use of a novel lysis chemistry eliminates the need for a lysate clearing step. Plasmid DNA purification is performed using a single plate and a simple bindwashelute...

Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins

Ni-NTA Superflow Columns enable up to 24 automated large-scale 6xHis-tagged protein preparations to be performed in parallel on the BioRobot 3000 workstation. Using cleared lysates from bacterial cultures as starting material, up to 15 mg protein per column can be purified under native or denaturing conditi...

MinElute PCR Purification Kit

For purification of PCR products in low elution volumes PCR product cleanup Spin columns 124 samples...

QIAquick PCR Purification Kit

For cleanup of DNA from PCR or other enzymatic reactions PCR cleanup Spin columns 124 samples...

Walkaway automation for nucleic acid purification

GenoVision AS, an innovative biotechnology company offering complete walkaway automation for the isolation and purification of nucleic acids, is now part of the QIAGEN Group of Companies and is now known as QIAGEN AS. QIAGEN AS focuses on low- to medium-throughput purification of DNA and RNA from a variety o...

RNAClean - RNA & cDNA in vitro Reaction Purification

Agencourts RNAClean kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic RT and IVT reactions such as cDNA and cRNA. The kit is formatted for microarray gene expression analysis experiments and is easily performed in manual or automated formats. RNAClean eliminates...

RNAPrep - Total RNA Isolation Purification

based magnetic bead purification kit for extracting and purifying total RNA from Eukaryotic cultured cells. RNAPrep is a simple and reproducible 3-step procedure for isolating total RNA for use in applications such as microarray and RT-PCR gene expression analysis. RNAPreps automation friendly...

RNA & cDNA Purification Using RNAClean

RNAClean is a SPRI -based nucleic acid purification system that efficiently isolates RNA or cDNA from in vitro transcription, cDNA or RNA labeling and antisense RNA amplification reactions. As gene expression profiling analysis and the use of microarrays and RNAi technologies grow, a highly effective, automated purific...

Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A

Ceramic Hydroxyapatite Chromatography, Rev A Henry Lai, PhD, and Samuel G Franklin, PhD, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Immunoglobulins (IgGs) fr...

Purification of Insulin on UNO S1 Column, Rev A

Insulin is a peptide hormone which stimulates anabolic reactions for carbohydrates, proteins, and fats resulting in lowered blood glucose. It is used therapeutically for treatment of diabetes mellitus and was the first recombinant protein on the market. Although insulin is well characterized and understood, it is often difficu...

Purification of Phosphoinositide 3-Kinase With UNO Columns

Polyphosphoinositides and their metabolites are important intracellular signals involved in the responses to a number of hormones and growth factors.The discovery of a phosphatidylinositol kinase that phosphorylates PI at the 3 position of the inositol ring uncovered a new pathway of PI metabolism and potential intracellula...

Purification of Recombinant Flavonone 3beta-Hydroxylase Using Bio-Scale CHT5-1 Hydroxyapatite Column

Flavanone 3b-hydroxylase (FHT), a typical 2-oxoglutaratedependent dioxygenase, catalyzes the 3b-hydroxylation of 2S-flavanones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavanoids, catechins, proanthocyanidins, and anthocyanidins in plants. To understand the complex reaction mechanism,...

Purification of rIL-13 Using an UNO Q1 Column

Interleukin 13 is a recently described cytokine produced by activated T cells. This protein is responsible for both positive and negative responses to regulatory molecules within the immune system. rIL-13 has been shown to modulate macrophage functions as well as play a direct role in regulation of proliferation and differentiation of hema...

Purification of Two Isoforms of Pyruvate Kinase from Nordic Krill Using an UNO Q1 Column, Rev A

Pyruvate kinase (PK, E.C. 2.7.1.40) is one of the key regulatory enzymes of the glycolytic pathway in crustaceans. This enzyme catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate and the phosphorylation of ADP to ATP. In addition to the role of PK in aerobic and anaerobic energy metabolism in...

Purification of the Components of Human Macular Carotenoid-Binding Complex, Rev A

The macular region of the human retina is responsible for almost all color vision and most of the high resolution visual acuity. This part of the retina, known as the macula lutea, or yellow spot, accumulates approximately 200 ng of two closely-related compounds, lutein and zeaxanthin....

Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins

Fang-Fang Wu, Xuemei He, and David R Nau, Bio-Rad Laboratories, Inc., Hercules, CA 94547 USA Immobilized metal affinity chromatography (IMAC) is a powerful technique that can be used for the efficient purification of recombinant histidine-tagged (His-tagged) proteins from a variety of ex...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

Contributed by James V. Anderson and Charles L. Guy, The University of Florida, Institute of Food and Agricultural Sciences BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated Proteins) are ER-luminal proteins and members of the HSP70 family. 1</...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

K. Sestak, L. Kim, K.-O. Chang and L. J. Saif, Food Animal Health Research Program, Ohio Agricultural Research and Development Program, The Ohio State University, Wooster, OH 44691 Three major structural proteins of the porcine coronavirus, transmissible gastroenteritis virus (TGEV), membrane (M), nu...

AMPURE PURIFICATION SYSTEM

AMPure is a high recovery, SPRI-based PCR clean up system capable of purifying amplicons as small as 100bp. AMPure yields high quality PCR amplicons using a simple, three-step protocol requiring no centrifugation or filtration steps. The protocol can be easily automated or performed manually in bot...

COSMCPREP HIGH AND LOW COPY PLASMID PURIFICATION

CosMCPrep is a versatile SPRI-based, plasmid purification system that uses a single protocol to purify a variety of high and low copy number template types. This flexible system is easily automated and produces high quality templates. The single protocol, ease of automation and high quality results make CosMCPrep an id...

SPRINTPREP SINGLE STEP PLASMID PURIFICATION

SprintPrep is an efficient SPRI-based plasmid purification system that can be completed in less than 15 minutes. Unlike most traditional plasmid purification chemistries, SprintPrep isolates plasmid DNA directly from overnight cultures, which can improve the turnaround time of a sequencing pipeline by as much as a full...

Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)

As the human genome sequence project is completed,the vast amount of genomic information calls formethods that allow gene expression analysis on agenomic scale and speed up detection of significantsequence variations. The most suitable tools available forgene express...

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

Lucia Martinez and Ario de Marco EMBL Protein Expression Core Facility, Meyerhofstr. 1, D-69117, Heidelberg, Germany Immobilized Metal ion Affinity Chromatography (IMAC) is an easy and reliable technique that allows the fast separation of His-tagged proteins from total lysates. There are several commercially available resins but all of them s...

Ultra-Fast Purification of Small Nucleic Acids

The flashPAGE Fractionator System is the fastest, easiest, and most consistent method for PAGE purification of small nucleic acids. Unlike traditional Polyacrylamide Gel Electrophoresis (PAGE), which was designed for the visualization of small nucleic acids, the flashPAGE Fraction...

Preparative Nondenaturing Gel Electrophoresis Used in the Purification of an Esterase Involved in Insecticide Resistance, Rev C

Dr Albert J Ketterman and SHP Parakrama Karunaratne, Dept. of MedicalParasitology, London School of Hygiene and Tropical Medicine, Keppel St,London WC1E 7HT UK Introduction In attempts to control Culex quinquefasciatus as a worldwide vector of diseases such and filariasis and encephalitis, mosquito field populati...

Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B

Contributed by Philip W Miller, Waltraud I Dunn, and Robert R Schmidt, University of Florida, Dept. Microbiology and Cell Science, Gainesville, FL 32611 USA Florida Agricultural Experiment Station Serial Number T-00256 Introduction One of the research goals of this laboratory is to elucidate t...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

K. H. Valkonen*, N. Marttinen*, H.-L. Malinen*, V.-P. Jaakola** and T. Alatossava* * Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, FIN-88600 Sotkamo, Finland ** Department of Biological and Environmental Science, University of Jyvskyl, PL 35, FIN-40351 Jyvskyl, Finland...

Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A

Jolanta Kolodziejek, MSc, and Professor Norbert Nowotny, PhD, Clinical Virology Group, Institute of Virology, University of Veterinary Medicine, Vienna , Veterinaerplatz 1, A 1210 Vienna, Austria The purity of the DNA template employed in sequencing PCR (cycle sequencing) is the most...

Whole Gel Eluter Purification of a Functional Multiprotein DNA Replication Complex, Rev A

, Philip W. Wills , and Linda H. Malkas , (a) Department of Pharmacology and Experimental Therapeutics (b) Program in Molecular and Cellular Biol...

Enzyme Purification With the Econo-Pac Q Cartridge

Data submitted by Dr. Ivan Kaiser, University of Wyoming,Laramie, WY USA Dr. Ivan Kaisers research involves the isolation and purification of proteins from snake venom, such as post-synaptic neurotoxins and hemorrhagic factors. In addition to research, Dr. Kaiser teaches a course on protein...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

Contributed by James V. Anderson and Charles L. Guy, TheUniversity of Florida, Institute of Food and Agricultural Sciences BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated Proteins) are ER-luminal proteins and members of the HSP70 family. BiPs...