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A New C-Terminal GST Vector for Protein Production in S. pombe
Stratagene Stratagene has constructed and tested the new pESP-3 vector, which can beused for C-terminal fusion with glutathione-S-transferase (GST) and for protein production in Schizosaccharomyces pombe....New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
Stratagene hybrid genes are used to detect protein-protein interactions via the activationof reporter gene expression. This expression occurs as a resul...Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
Danny Q. Hoang Michael Browning Xinping Yang PeterPingerelli Stratagene Cloning Systems, Inc. Stratagene introduces affinity purified antibodies for NMDA receptorsubunits that are useful for studying expression of the NR2A, NR2B and NR2Csubunit proteins...Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
-TaggedFusion Proteins Qiyi Xie Quinn Lu Tanya Hosfield Peter Pingerelli Weiping Yang Stratagene...New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
Stratagene Cloning Systems, Inc. The ESP LIC cloning and expression system features rapid and high-yieldproduction of recombinant proteins in yeast. This system usesligation-independent cloning (LIC) for efficient and dire...Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
Brilliant Blue Dye Stratagene Cloning Systems, Inc. Stratagene currently provides an optical bandpass filter with all EagleEye II still video systems that is...High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
Epitope Tagging in E. coli Katherine Felts Denise Wyborski John Bauer PeterVaillancourt Stratagene LIC cloning kit...Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
Department of Cell Biology, Neurobiology, and Anatomy University of Cincinnati College of Medicine, Cincinnati, OH 45267 Transfection of mammalian cell lines is enhanced by the use ofliposome-based reagents. Here we compare the transfection e...Yeast Protein Production System Features High Yields and One-Step Purification
The ESP system provides high-yield protein production in a eukaryoticorganism Quinn Lu * Bruce Jerpseth * Tim Sanchez * John C. Bauer * AlanGreener Stratagene Cloning Systems, Inc. provides novel opportunities for rapid, inexpensive and high-yield p...Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
Recombinant Enterokinase for highly specific digestion of purifiedproteins or proteins in crude extracts Ed Marsh * Johanna Claycomb * ChaoFeng Zheng * Denise Wyborski* John C. Bauer * Joseph A. Sorge * A.K. Bernardino * Peter Vaillancourt Stratagene Cloning Systems, Inc. Stratagene introduces recombinant Enterokinase, an efficient, sitesp...Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
Denise L. Wyborski * John C. Bauer * Barbara McGowan * JosephA. Sorge * Peter Vaillancourt Stratagene Cloning Systems, Inc. Stratagene has improved the Affinity protein expression andpurification system by the addition of the pCAL-n-EK vector. This E. coliclonin...pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
Faster, easier screening for difficult transfections when using thecomplete control kit Stratagene We describe the vector pFB-ERV, an MMLV-based replication-defectiveretroviral vector for delivery of the ecdysone receptor proteins RXR and VgEcR.In stable cell lines infected with an estima...Codon Bias-Adjusted BL21 Derivatives for Protein Expression
Stratagene BL21-Gold cells * called the BL21-CodonPlus -RILseries** of strains, which contain extra copies of the E. coli argU,ileY, and leuW tRNA genes. This mo...TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
Institute for Molecular Biology and Biotechnology, McMaster University,Ontario, Canada GST-fusion proteins expressed in TKB1 bacteria are used to affinity purifycellular proteins, which interact with a specific autophosphorylation site onthe Erb...High-efficiency Derivatives of the BL21 Series for Protein Expression
Melody Jerpseth Bruce Jerpseth Lisa Breister AlanGreener Stratagene Stratagene has created variants of BL21 competent cells, the new BL21-Goldseries. Epicurian Coli competent cells lack the EndA1 nuclease...Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins
Ni-NTA Superflow Columns enable up to 24 automated large-scale 6xHis-tagged protein preparations to be performed in parallel on the BioRobot 3000 workstation. Using cleared lysates from bacterial cultures as starting material, up to 15 mg protein per column can be purified under native or denaturing conditi...Characterization of Protein Oligosaccharides Using MSn
Andrew S.Weiskopf and Paul Vouros, Northeastern University, Boston, MA David J. Harvey, University of Oxford, Oxford, UK The data presented here can be acquired using the Thermo Finnigan LCQ Series of ion trap mass spectrometers. Introduct...Michaela Scigelova, Gary Woffendin; Thermo Finnigan, Hemel Hempstead, UK. Malcolm Ward, Helen Byers; Proteome Sciences, London, UK. Diane Hanger; Institute of Psychiatry KCL, London, UK. The data presented here c...
Fang-Fang Wu, Xuemei He, and David R Nau, Bio-Rad Laboratories, Inc., Hercules, CA 94547 USA Immobilized metal affinity chromatography (IMAC) is a powerful technique that can be used for the efficient purification of recombinant histidine-tagged (His-tagged) proteins from a variety of ex...
Contributed by James V. Anderson and Charles L. Guy, The University of Florida, Institute of Food and Agricultural Sciences BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated Proteins) are ER-luminal proteins and members of the HSP70 family. 1</...
K. Sestak, L. Kim, K.-O. Chang and L. J. Saif, Food Animal Health Research Program, Ohio Agricultural Research and Development Program, The Ohio State University, Wooster, OH 44691 Three major structural proteins of the porcine coronavirus, transmissible gastroenteritis virus (TGEV), membrane (M), nu...
Evelyn McGown, Ph.D., Jinfang Liao, M.D., Ph.D., Mary Kassinos, Irina Osetinsky and Jayne Hesley, Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. They can be cloned in a diverse range of cells and org...
1 GFP (Green Fluorescent Protein) is an extremely stable protein of 238 amino acids produced by the jellyfish that fluoresces in the lower green region of the visible spectrum....
By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the NanoOrange Pro software from Mo...
Protein concentration is commonly estimated by measuring the absorbance at 280 nm and calculating the concentration using the extinction coefficient of the protein. The procedure is feasible because extinction coefficients are typically obtained using standard cuvettes with a fixed 1 cm pathlength. Extinction-based protein assays can also...
Jinfang Liao, M.D., Ph.D. and Evelyn McGown, Ph.D. Molecular Devices Corporation, 8/00 The photoprotein aequorin, a bioluminescent protein from the coelentratejellyfish Aequorea victorea, has proven to be highly sensitive to the trace amount of Ca in cells. The aequorin complex which contains the...
Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
The endopeptidase originally isolated from the filtrate ofthe lower fungus Tritirachium album limber is recombinantlyexpressed in Pichia pastoris. Proteinase K isextremely effective on native proteins from differentsources, and remains active in the presence of denaturingreag...Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
MIA (Melanoma Inhibitory Activity) was cloned as asecreted protein from human melanoma cell lines [1]. MIAis expressed in and secreted from malignant melanomasand chondrocytes [2, 3]. As MIA expression in chondrocytesis dependent on the differentiation status of the cel...This Application Note describes how the Agilent 2100 bioanalyzer andthe Cell Fluorescence LabChip kit can be used to determine the efficiencyof transfection of mammalian cells using green fluorescent protein(GFP) as a reporter molecule. Transfection of CHO-K1 cells with...
This Application Note describes how the Agilent 2100 bioanalyzer andthe Cell Fluorescence LabChip kit can be used to detect cell surfaceand intracellular protein targets by antibody staining. CD3 staining onJurkat cells as well as CD4 staining on CCRF-CEM cells and intrace...
Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer The Agilent 2100 bioanalyzer is aunique, on-chip, electrophoresis andflow cytometry analysis tool for RNA,DNA proteins and cells....
Comparison of different protein quantitation methods
This application note compares the accuracy and reproducibility ofabsolute protein quantitation of the Agilent 2100 bioanalyzer in combinationwith the Protein 200 Plus assay to the batch-based methodsLowry and Bradford as well as to denaturing gel-elctrophoresis (SDSPAGE)....Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
Antibodies are commonly used in diagnostics, as research tools(for example protein arrays) or as biopharmaceutical therapeutics. Inall cases the successful production of antibodies requires precise testingand quality control. The key quality criteria include: protein ident...Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
Whereas in the past mass spectrometers (MS) were confined to therealm of small molecules, the development of soft ionization methodssuch as matrix-assisted laser desorption/ionization (MALDI) and electrosprayionization (ESI) revolutionized the analysis of large biomolecule...Lucia Martinez and Ario de Marco EMBL Protein Expression Core Facility, Meyerhofstr. 1, D-69117, Heidelberg, Germany Immobilized Metal ion Affinity Chromatography (IMAC) is an easy and reliable technique that allows the fast separation of His-tagged proteins from total lysates. There are several commercially available resins but all of them s...
A Better Way to Isolate RNA AND Protein From the Same Sample
Isolate nuclear and cytoplasmic RNA and protein from cultured cells Ambion's new PARIS Kit uses a fast, simple procedure based on Ambion's RNAqueous technology to isolate bo...Efficient Cellular Fractionation for RNA and Protein Isolation
Efficient Cellular Fractionation for RNA Ambion's Protein and RNA Isolation System, PARIS, uses a rapid and simple procedure to isolate both total RNA and native protein from the same experimental sample. Cultured cells and tis...Increase Protein Activity and Yield
Higher yields of protein than reticulocyte lysate systems lysate systems Easy to...Isolate It All: siRNA miRNA Total RNA Native Protein
, Ambion, Inc Isolate total RNA and native protein from the same sample Enrich for small RNAs ( Ideal for assessing siRNA or miRNA expression and processing Small interfering R...Isolate Total RNA and Protein From the Same Sample
Vana PARIS Kit combines the technological advances from both the <A href="http://www.ambion.com/catalog/CatNum.php?1560">mir <A href="http://www.a...Other TagsAssociateAssociateAssociateAssociateULTRAMERDOVERMidstreamCURITYCURITYBladderScanOther Contentsbowlegsretrainingretrainingbracebracebracebracebradycardiabradycardiaplexopathyaneurysmaneurysmaneurysm
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Breaking Biology News(10 mins):NSAIDs: Take 'em early and often when competing? Think again 2iQor Named to InfoWorld 100 List of Top IT Projects of 2009 2IgeaCare Solutions Inc. Emerges Under New Leadership and Funding 2Aware Software and Development Services Enable State of the Art Fingerprint Card Scanning 10015 1Aware Software and Development Services Enable State of the Art Fingerprint Card Scanning 10015 2Aware Software and Development Services Enable State of the Art Fingerprint Card Scanning 10015 3September October 2009 Annals of Family Medicine tip sheet 57891 1September October 2009 Annals of Family Medicine tip sheet 57891 2September October 2009 Annals of Family Medicine tip sheet 57891 3September October 2009 Annals of Family Medicine tip sheet 57891 4September October 2009 Annals of Family Medicine tip sheet 57891 5September October 2009 Annals of Family Medicine tip sheet 57891 6Toxin detection as close as an inkjet printer 14194 1Toxin detection as close as an inkjet printer 14194 2(Date:12/16/2009)... of C3: Colorectal Cancer Coalition,s advocacy efforts, $15 million ... included in the final Fiscal Year 2010 Defense Appropriations ... research cancers, like colorectal cancer, that are not addressed ... programs run by the U.S. Army Medical Research and ... funding for this new cancer research program was included ...
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Breaking Medicine News(10 mins):Health News:House Creates New Cancer Research Program 2Health News:House Creates New Cancer Research Program 3Health News:New Stroke Tool May Predict Early Recurrence 2Health News:Gene Variant Seems to Guard Against Asthma, COPD 2Health News:Gene Variant Seems to Guard Against Asthma, COPD 3Health News:Behavior modification could ease concerns about nanoparticles 2Health News:New study links DHA type of omega-3 to better nervous-system function 2| Copyright © 2003-2009 Bio-Medicine. All rights reserved. ABOUT | CONTACT US | DISCLAIMER | PRIVACY POLICY | TERMS AND CONDITIONS |  |