Product

A New C-Terminal GST Vector for Protein Production in S. pombe

Stratagene Stratagene has constructed and tested the new pESP-3 vector, which can beused for C-terminal fusion with glutathione-S-transferase (GST) and for protein production in Schizosaccharomyces pombe....

Safe Production of High-Titer Retrovirus

Stratagene We describe a set of vectors that can be used with any MMLV-basedretroviral vector to produce viral supernatants that have titers that areconsistently greater than or equal to 10 cfu/ml...

Production of Full-Length, Biologically Active MEK1WT and MEK1CA

Stratagene Cloning Systems, Inc. Stratagene has successfully expressed and purified recombinant, wild-typehuman MEK1 )using the ESP yeast pro...

Yeast Protein Production System Features High Yields and One-Step Purification

The ESP system provides high-yield protein production in a eukaryoticorganism Quinn Lu * Bruce Jerpseth * Tim Sanchez * John C. Bauer * AlanGreener Stratagene Cloning Systems, Inc. provides novel opportunities for rapid, inexpensive and high-yield p...

Identification of Differentially Expressed Gene Products with the CastAway System*

CastAway precast gels for fast analysis of RAP-PCR and differentialdisplay Stratagene Cloning Systems, Inc. Stratagenes new CastAway precast sequencing gels were used to detectand isolate differentially expressed gene products amplified by the RNAarbitrarily primed polymerase chain reaction (R...

High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications

Simultaneous purification of 96 PCR products Stratagene Phenogenex 96 PCR purification kit is a newmember of the StrataPrep product line. This kit offers a high-throughput formatfor purifying ultra-pure PCR product DNA from la...

cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR

RT-PCR has become an essential method that allows researchers to determine the presence, structure and level of expression of an mRNA transcript in the organism, tissue or cell of interest. The basic protocol involves the synthesis of a cDNA copy of all the mRNA present in the s...

Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray Ion Trap Mass Spectrometry

Andreas Hhmer, Helen Tran, and William S. Hancock The data presented here can be acquired using any Thermo Finnigan LCQTMion trap mass spectrometer with MS...

Advantages of Roche Applied Science amplification products

The Polymerase Chain Reaction invented by Kary Mullis in 1983 drastically changed the course of modern life science research by opening an enormous variety of new opportunities to study and understand the crucial role of nucleic acids in living organisms. Roche Applied Science recognized ea...

Find the optimal product for your PCR application

Which of the following statements best describes your template/starting material: <a href="http://www.roche-applied-science.com/pcr/p...

Product overview

Below please find an alphabetical list of key Roche Applied Science products for nucleic acid amplification. For detailed information about a product please follow the respective link. Protector RNase Inhibitor...

Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A

Joseph W. Turek and Guy C. Le Breton, Department of Pharmacology,University of Illinois at Chicago, College of Medicine, Chicago, IL 60612.Address correspondence to: Joseph W. Turek, 835 South Wolcott, Departmentof Pharmacology M/C 868, University of Illinois at Chicago, Chicago, IL 60612.E-mail: jturek1@icarus.uic.edu Introduction...

New MailFoundry product fights latest spam techniques

and <a href="http://www.tech-faq.com/brute-force-attack.sht...

Tax consumption over production to reform Wisconsins tax system

MADISON The supporters of the Taxpayers Bill of Rights are right about one thing: Wisconsin needs a new way of raising and spending money on state and local government. Our tax system hampers economic growth and theres too much overlap between units of government. But the opponents of TABOR are right about one thing, too: A formula-driven solution to controlling taxes and spending coul...

PKWare launches new enterprise product line, adds new features to PKZip

Brown Deer, Wis. Information technology professionals often face the challenge of delivering cost-effective and secure data management solutions designed to maximize both storage and bandwidth efficiency, while providing seamless, easy-to-use solutions for their users. PKWare has announced SecureZip, a new family of products designed for enterprise customers that seek encryption and authenticati...

Trouble-shooting: No product

Here are some troubleshooting hints that we have gathered if your PCR yields no product: Non-optimal Mg...

Find the optimal product for your application

Roche Applied Science (RAS) provides an extensive product line for amplification. For a quick overview you can browse through the . Although all products will perform in a broad array of applications, we would like to point out particul...

Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori

Gastric infection with Helicobacterpylori (H. pylori) is the majorcause of chronic active gastritisand is associated with the pathogenesisof peptic ulcer and gastriccarcinoma. Recent studies using amultiplex PCR (mPCR) basedapproach distinguished differental...

GENE SILENCING WITH PCR PRODUCTS

siRNA expression vectors offer enormous opportunities to study gene silencing. Unfortunately, the preparation of siRNA vectors is labor and time intensive. Ambion's new Express siRNA Expression Cassette Kits pr...

Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A

Menu B. Leddy, Biotechnology Research Department,Orange County Water District, 10500 Ellis Ave., Fountain Valley, CA 92708 Identification and characterization of bacterial species from membrane biofilms has been a microbiological challenge. A vast majority (>90%) of bacterial cells from membrane...

Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A

Jolanta Kolodziejek, MSc, and Professor Norbert Nowotny, PhD, Clinical Virology Group, Institute of Virology, University of Veterinary Medicine, Vienna , Veterinaerplatz 1, A 1210 Vienna, Austria The purity of the DNA template employed in sequencing PCR (cycle sequencing) is the most...

Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A

Marni Brisson, Larissa Tan, Rob Park, and Keith Hamby,Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Dr., Hercules, CA 94547 USA Real-time PCR* is a powerful and effective technique for accurate quantitation of DNA. Assays for the detection of a single gene involve careful choice of primers, tar...

Imaging and Analysis of DIGE Product Information Sheet, Rev B

Difference gel electrophoresis (DIGE) is a relatively straightforward application of differential labeling of protein samples using fluorescent dyes. The technique, originally published by Unlu et al. (1997), uses cyanine (Cy) dyes to differentially label proteins separated by 2-D gel electrophoresis. Following labeling...

Production of Hybridomas by Electrofusion

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Production of Hybridomas by Electroporation

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Increase Your Organizations Productivity with LabVIEW

This paper addresses managers in different industries and describes the benefits of implementing their applications in LabVIEW. It describes how LabVIEW increases productivity, and touches upon subjects such as ease of use, complete functionality, available tools and resources, and performance. This paper also describes the main criteria managers should consider when choosing th...

Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS

The European Union prohibits the use of chloramphenicol (CAP) as a veterinary drug for food producing animals. For this reason a rapid and sensitive confirmatory method for the identification and quantification of CAP in milk and milk products has been developed using the API 3000 LC/MS/MS System. Although many analytical procedures are describ...

Using THERMAL DESORPTION GAS CHROMATOGRAPHY for the identification of fragrance and other components in cosmetic products

Because of the solid or viscous nature of these formul...

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

Over the last several decades, geneticists have learned how to manipulate DNA to identify,excise, move and place genes into a variety of organisms that are quite different genetically fromthe source organism. A major use for many of these recombinant organisms is to produceprot...

Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production

By J. Doyle, L. Johnstone and L. Cottis AGEN Biomedical Ltd. Queensland, Australia http://www.agen.com.au The immobilized-bed perfusion was investigated as a means for producinglarge quantities of MAb from a hybrid...

WARF enters licensing agreements for stem cell products

The agreements mark the beginning...

Surgical products company wins Governor's Business Plan Contest

Vector Surgical, the winner in the life sciences category, be...

Merge unveils next-generation imaging-informatics product

Merge, a medical imaging software and services company, will market the product, which is called Fusion RIS/PACS, to im...

DeForest biodiesel plant begins production

will operate the plant and produce 60,000 gallons of biodiesel per day from a variety of different raw materials, including beef tallow. The plant, which is served by road and rail transport...

Study affirms information technology-productivity link

The recent report titled Digital Pro...

Fusion 2007: Innovation drives productivity in post 24x7 world

You can't work 25 hours a day. So several speakers at the Fusion 2007 CEO-CIO Symposium on Tuesday and Wednesday tried to drive home the point that "work smarter, not harder" is more than just an age-old mantra. Michael Knetter, dean of the University of Wisconsin-Madison School of Business, summed it up: "When productivity grows, our standard of living goes up," Knetter said. To him, pr...

GE Healthcare introduces MR imaging product

The product, called CartiGram, was on display at the annual meeting of the <a href="http://www.aaos....

Wisconsin court halts Abbott's sale of genotyping products

, preventing the pharmaceutical company from further sales or use of products or components that infringe on a genotyping patent held by <a href="http://www.innogenetics.com...

Madison life-science firms agree to co-develop kidney product

, have entered into a license and supply agreement to commercialize products to treat...

Renovar captures $1.2M for product development

, which has raised a total of $3.2 million since its inception in 1999, will use the proceeds from its second financing ro...