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Primer in Biological Technology

Human t-PA Control Primer Set

Catalog Number Pack Size 1691104 Set Package Inserts/Product Instructions ...

General Notes on Primer Design in PCR*

Vincent R. Prezioso, PhD BioSystems Laboratory, Brinkmann Instruments Inc., Westbury, New York Perhaps the most critical parameter for successful PCR is the design of Primers. All t...

RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

Primer sets facilitate study of DNA mismatch repair Mark Dycaico Stratagene Cloning Systems Stratagene has designed RT-PCR primer sets from the cDNA sequences of mismatch repair genes. These genes code for specialized proteins that participate in correcting areas of ...

New Report Just Published: World Prostate Cancer Therapeutics Market Report

... 2004 (includes corresponding Graph/Chart) II-18 Prostate - Structure II-18 Pituitary Gland II-19 Testes II-19 Prostate Cancer - A primer II-20 Key Facts to Gaze at II-20 Table 13: Lifetime Probability of Developing Cancer (in Men) by Type in the US, 2002-2004 II-20 Tab...

Event Alert: 'SPL R4 Compliance and its Impact on Regulatory Submission Processes'

...ompanies who are modifying in-house systems or evaluating new technologies to meet the new SPL R4 requirements. The webinar is designed as a "how to" primer on complying with SPL R4. Attendees will learn about: Best practices for complying with SPL R4 Accelerating the convers...

Waters and University of Warwick Sign Research Agreement Designed to Foster Adoption of Novel MS Technologies

... for life scientists adopting cutting edge MS-based research strategies." For more information on MS technologies, Waters recently published a MS primer complete with descriptions on modern MS practices, who uses mass spectrometers, the various types of MS, the importance of mass accuracy and resolutio...

Jivan Focuses on Solutions for Transcriptome Sequencing

...content for targeted sequencing with mRNA capture arrays, mRNA specific PCR primer designs for targeted amplification, and software for analyzing mRNA express...or genes or mRNAs with low expression, Jivan will offer mRNA Prime(TM) PCR primer designs specific to each observed mRNA. To analyze RNA sequencing data...

Volatile Economy / Fundraising Challenges Encourage Business Combinations Among Biotech Companies According to New Survey from CPA Firm Rothstein Kass

... the topic, and a highly-sought counselor to families with significant global resources, and their advisors. He is co-author of Fortune's Fortress: A primer on Wealth Preservation for Hedge Fund Professionals . www.RussAlanPrince.com Hannah Shaw Grove is a widely recognized author, columnist, spea...

RainDance Technologies Delivers RDT 1000, Sequence Enrichment Solution to JCVI

... RainDance delivers its RDT 1000 and expert training to selected premier organizations in genomic research. RainDance will also provide customized PCR primer libraries designed to selectively amplify loci of interest for research initiatives at JCVI. "The program provides an opportunity for some of the worl...

Gerson Lehrman Group and BIO Collaborate to Identify Scientific Expertise for the Seventh Annual BIO Investor Forum

...ibrosis -- Epilepsy -- Fibromyalgia -- Lung Cancer -- Neurodegenerative Diseases -- Novel Solid Tumor Targets -- Osteoporosis -- Pre-ASH primer "With the financial markets experiencing an unprecedented level of volatility, investment professionals place a greater premium than ever before on s...

Norgen Biotek Corp. Launches Four New Kits for the Purification of RNA and DNA from Plants and Fungi

...ty, and can be used in a number of downstream applications including Real-Time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. With the Plant/Fungi DNA Isolation Kit total DNA is isolated including genomic DNA, mitochondrial DNA and chl...

Newsweek International Editions: Highlights And Exclusives, May 19, 2008

...w To Stop The Food Riots. (Atlantic and Latin America editions). Senior Editor Rana Foroohar opens this special report on the world food crisis with a primer on the incredibly political nature of food. As food prices spiral out of control, the worry is that millions more of the world's poorest will also be ...

febit to Introduce Geniom RT Analyzer(R) to U.S. Market at RNAi World Congress

... temperature control optimizes hybridizations and enables the use of enzymatic reactions directly on the microfluidic array for signal enhancement and primer extension assays. "We have brought together in one benchtop tool the automation of multiple processes involved with the identification and understan...

Gentelligent, Inc. Asks 'Is your DNA in Excel?'

...time; it's kind of surprising that we're the first company to bring these functions to Excel." BasePro allows users to do everything from determining primer melting temperatures and molecular weights to automatically expanding and contracting IUPAC codes and identifying sequence repeats. According to co-fo...

Growing Venture Capital in Wisconsin

...ourcebook to growth and venture capital for Wisconsin companies. The Wisconsin Technology Network received an advance copy of the 60-page report, a primer for Wisconsin companies seeking information about risk capital available in our state. It shows that Wisconsins economy lags behind neighbors Illinois...

Why Wisconsin Lags Behind the U.S. and Minnesota

...ween newsletters. The Wisconsin Technology Network has received from NorthStar Economics an advance copy of its 60-page report, which contains a primer for Wisconsin companies seeking information about risk capital available in the state. David Ward and his team have agreed to exclusively excerpt the...

Angel Investing in Wisconsin

...dvance copy of NorthStar Economics 60-page report titled, the NorthStar Guide to Growth and Venture Capital in Wisconsin . This report contains a primer for Wisconsin companies seeking information about risk capital available in the state. David Ward and his team have agreed to excerpt their report in ...

Community Development Venture Capital

...ve advance copy of NorthStar Economics 60-page report titled, the NorthStar Guide to Growth and Venture Capital in Wisconsin. This report contains a primer for Wisconsin companies seeking information about risk capital available in the state. David Ward and his team have agreed to excerpt their report in ...

Seed-Stage Venture Financing

... The Wisconsin Technology Network has received from NorthStar Economics an advance copy of its 60-page report, which contains a primer for Wisconsin companies seeking information about risk capital available in the state. David Ward and his team have agreed to exclusively excerpt thei...

The Complexities, Cost of Midwest Biotech Drug Development

...-up biotech companies, most people now understand that it is a long, painstaking and expensive process. What they don't understand is why. A brief primer in the drug discovery and development process shows us the following pathway: Logical questions to the above include the following: If th...

Corporations and blogging

...t someone else will. If you're just beginning to think about blogging in the corporate environment, I highly recommend Dan's book. It's a marvelous primer and great scout that will help you make smart decisions about using and working in the blogosphere. Chris Shipley is the executive producer of Net...

Why file for trademark protection?

...01 bursting of the Internet bubble). While trademarks are important to businesses, many business people don't know much about them. Here is a brief primer on trademark law to help steer you towards choosing the right trademark and protecting it well. A trademark is any word, phrase, shape, design, c...

Is Google's cafeteria a competitive weapon?

...re they get it. Food is even a part of Google's recruiting strategy for top engineering talent. Google Lab's for example, includes a witty two-page primer in its recruiting kit entitled, "How to Care for Your Big, Wonderful High-Performance Brain." At the top of the list of recommendations it makes is ea...

Prodesse joins forces with Invitrogen

...forms, to laboratories throughout the world. The agreement provides for the incorporation of LUX into Prodesse reagents as a different way to label primer sets. "It enables us to do melt curves," said Andy Shrago, chief marketing officer for Prodesse. To discriminate detections, researchers can mea...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

... primers, and does not suffer from reduced amplification due to competing primer interactions. There are limits to all WGA techniques, and tissue f...ary of amplifiable template molecules by relying upon a simple degenerate primer extension, it alone is poised to amplify DNAs that are highly fragmented ...

QIAGEN Fast Cycling PCR Kit

...tivity successful results with minimal optimization Easy and convenient handling optional ready-to-load PCR buffer and no primer redesign required Ultrafast hot-start PCR The innovative ...

FlexiPlate siRNA

...are ideal for high-throughput screening. QuantiTect primer Assays are ready-to-use primer sets for quantitative, real-time RT-PCR using SYBR Green based...

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

...tion D2. The Premix contains enzyme, dNTP's, and ddNTP's and is supplied in the DYEnamic Kit. Prior to the run, this was manually mixed with 5 μM primer (M13 Forward) and water. All Sciclone ALH 3000 movements were performed at 100% travel speed except when entering and exiting liquids. Liqui...

Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

... OliGreen Oligonucleotide Quantitation Reagent from Molecular Probes (Cat.# O-7582) Oligonucleotide standard: an M13 sequencing primer from Molecular Probes (Cat.# O-11492) Black 96-well plate Brown eppendorf tubes Spectrofluorometer (Gemini XS,...

Counting FlashPlates on the Wallac MicroBeta

...Reverse Transcriptase Assay was performed using a Streptavidin coated FlashPlate (SMP103). In this assay, a biotinylated DNA oligonucleotide acts as a primer for reverse transcriptase (RT) on an RNA template. The signal is generated by incorporation of labeled dTTP into the newly synthesized strand of DNA. ...

Amplification Efficiency of TaqMan Gene Expression Assays

...Expression Assays have been designed by the Applied Biosystems genome-aided primer and probe design pipeline. Design parameters such as %GC content, Tm, and a...ulate a C T slope. When using custom designed TaqMan probe and primer sets that are not a part of the TaqMan Gene Expression Assays product line,...

Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

...quent-cutting enzyme (anchoring enzyme AE). Using streptavidin-coated beads, the 3' end of the cDNA is bound and then split into two pools, A and B. A primer (A' and B') containing a recognition site for a type IIS restriction enzyme is ligated to the nucleotides in each of these pools. 2.Followi...

Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

...timization step may be necessary to identify those primer combinations that will generate sufficient unique ...kit allows researchers to choose from 128 possible primer combinations that have been tested across several ... DNA Analyzers, in conjunction with optimized AFLP primer kits and GeneMapper Software v3.7 or higher, provi...

Simple Approaches for Optimization of RT-PCR

...tention is also paid to fundamental issues such as primer design (6,7) , RNA isolation (8,9) , and use of RN... (total RNA: 1 pg to 1 μg) Forward primer (10μM) 2 μl (0.4μM in 50 μl reaction) Reverse primer (10μM) 2 μl (0.4μM in 50 μl re...

A simple method to sequence from bacterial colonies using [a-33P] radiolabeled ddNTPs and Thermo Sequenase

...her endlabeled primers or by the incorporation of radiolabeled dNTPs during primer extension. Successful sequencing results produced by these two labeling met... visualization of sequencing artifacts because only specifically-terminated primer extension products are labeled. The Thermo Sequenase Radiolabeled...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

... ◊ RiboAmp TM RNA Amplification Kit (Arcturus, Cat. # KIT0201) ◊ Slow Myosin MyHC Antibody NOQ7-5-4D ◊ PCR primer (Invitrogen, custom-made) ◊ Goat Serum (Sigma, Cat. # G-9023) ◊ Ethanol (Pharmaco, Cat. # 111ACS200) ◊...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

...ons (Applied Biosystems) and combined with the appropriate volume of Taqman master mix. Highly reproducible results were obtained using 900 nM of each primer and 250 nM of probe. 3. 22.5 L of the master mix was loaded for each gene of interest on a 96-well optical reaction plate, and 2.5 L of cDNA ...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...logies, Carlsbad, CA) in optical tubes. 2. Add 50nM of the forward primer and reverse primer, and 100nM of the probe to the master mix for each ...ecule1 (ICAM-1), and monocyte chemoattractant1(MCP-1) (see Table I for gene primer sequences). As shown in Table II, LPS stimulation increased the expression ...

GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH

...strategies being used for the generation of genomic arrays. In this process, a genomic DNA clone is digested with a restriction enzyme and a universal primer adaptor is ligated to serve as a priming site for PCR amplification. A different method employs degenerate oligonucleotide-primed PCR (DOP-PCR) to ran...

Oragene and DNA sequencing with ABI PRISM

...according to protocols supplied with the Oragene kit. primer design PCR primers for the human Thymidylate Synthase (TYMS) g...1178). The primers generate a 560-bp fragment. Table 1 shows the primer sequences. DNA Sequencing Oragene-purifi...
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