Polymer

Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

pfuturbo DNA polymerase for robust, high-fidelity PCRamplification Holly Hogrefe Brad Scott Kirk Nielson ValerieHedden Connie Hansen Janice Cline Frances Bai Jeff Amberg RondaAllen Mark Madden Stratagene an improved version of Pf...

Pfu DNA Polymerase as a Hot Start Alternative

Stratagene Cloning Systems, Inc. This article describes the important, novel application of Stratagenes PfuDNA polymerase for minimizing PCR cold start mismatched primer-extensionreactions. These mismatched extension rea...

PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases

pfuturbo DNA polymerase is the superior choice for robusthigh-fidelity PCR amplification Stratagene In this update, we compare the PCR performances of pfuturbo DNApolymerase * ,</...

pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning

A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning Stratagene was cloned into the PC...

Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications

Herculase enhanced DNA polymerase delivers high fidelity and greatperformance Stratagene Stratagene has recently released Herculaseenhanced DNApolymerase,* a novel enzyme formulation that providessuperior performance in both routine and demanding PCR applications. Compariso...

Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase

DNAPolymerase Stratagene DNA polymerase* by making simple modifications to PCR reactionconditions. In this update, we d...

High-Fidelity PCR with a Novel Polymerase Mixture

New polymerase mixture, TaqPlus Precision PCR system, developed forhighfidelity PCR Kirk B. Nielson * Janice Cline * Frances Bai * Daniel McMullan* Barbara McGowan * Holly Hogrefe Stratagene Cloning Systems, Inc. Stratagene announces the addition of the TaqPlus Precision PCR system,* to an expanding line...

Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer

Optimizing pfuturbo DNA Polymerase Amplification Reactions withPerfect Match Stratagene Certain PCR amplification systems produce multiple pro...

Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance

Improve reliability of PCR reactions with Herculase enhanced DNApolymerase Stratagene enhanced DNApolymerase,* a novel enzyme formulation that providessuperior performance in both routine and demanding PCR applications. Previous...

The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA

Max-Planck-Institute for Marine Microbiology, Celsiusstr.1, 28359 Germany Phone: ++49-421-2028-936, Fax: ++49-421-2028-580, email: kravensc@mpi-bremen.de Direct extraction of total DNA from soils or sediments always results in...

HotStarTaq DNA Polymerase

For robust amplification in all applications HotStarTaq DNA Polymerase...

C. therm. Polymerase One-Step RT-PCR System

for one-step RT-PCR amplification of up to 3 kb RNA targets Cat. No. 2 016 338 50 reactions Cat. No. 2 016 346 250 reactions The system contains a unique enzyme blend of C. therm. Polymerase and Taq DNA Polymerase. Note: C. therm. Polymerase prov...

FastStart Taq DNA Polymerase

for "Hot Start" PCR amplification of up to 3 kb RNA targets Cat. No. 2 158 264 50 units for 25 PCR reactions Cat. No. 2 032 902 100 units for 50 PCR reactions Cat. No. 2 032 929 2 250 units for 250 PCR reactions Cat. No. 2 032 937 4 250 units for 500 PCR reactions Cat. No. 2 032 945 10 250 units for 1250 P...

Taq DNA Polymerase

for routine amplification of up to 3 kb genomic DNA targets Cat. No. 1 647 679 250 units (1 unit/l) Cat. No. 1 647 687 4 x 250 units (1 unit/l) Cat. No. 1 146 165 100 units (5 units/l) Cat. No. 1 146 173 500 units (5 units/l) Cat. No. 1 418 432 4 x 250 units (5 units/l) Cat. No. 1 596 594 1...

C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR

Polymerase for two-step RT-PCR amplification of up to 4 kb targets C. therm. Polymerase provided by Roche Applied Science is the recombinant form of the DNA polymerase from the thermophilic eubacterium Carboxydothermus hydrogen...

Acrylamide Polymerization A Practical Approach

Paul Menter, Bio-Rad Laboratories, 2000 Alfred Nobel Drive,Hercules, CA 94547 USA The unparalleled resolution and flexibility possible with polyacrylamide gel electrophoresis (PAGE) has led to its widespread use for the separation of proteins and nucleic acids. Gel porosity can be varied over a...

Pwo DNA Polymerase

for routine high fidelity amplification of up to 3.5 kb DNA targets Cat. No. 1 664 947 100 units Cat. No. 1 664 955 2 x 250 units Pwo DNA polymerase was originally isolated from hyperthermophilic archaebacterium...

Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System

FastStart Taq DNA Polymerase, a chemically modified versionof Taq DNA Polymerase for hot start PCR, was introducedin 2000. Based on the feedback from many customers,FastStart Taq DNA Polym...

PCR Polymerases Application Profiles

Taq DNA polymerase is still the most commonly used thermostable polymerase for PCR today. However, Taq polymerase has serious limitations like the lack of an error-correcting activity and can only be used for amplification of rather short sequenc...

Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye

Mimi Amutan and David Batey, Ph.D. Real-time quantification was performed for lambda and human genomic DNA on the DNA Engine Opticon system (MJ Research). DyNAzyme II DNA polymerase*was used for amplification, and SYBR Green I fluorescent dye was used for detection. The sensitivity and linear dynamic range of...

Polymer Degradation Mechanisms Encountered in Pyrolysis-GC/MS

465 Limestone Road Oxford, PA 19363-0277 with gas chromatography/ mass spectrometry (GC/MS) to study the structure of polymeric material must be based on an unders...

Polymer grid technology a boon for bridges

When the long-awaited Highway 151 bypass around Fond du Lac, Wis., opens later this year, vehicles traveling northbound will cross DeNeveu Creek on a bridge like no other in the country. Externally, the bridge looks identical to its adjacent twin. However, intern...