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Plasmid in Biological Technology

High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation

James Batchelor, Lynn Jordan, and Ed Alderman Applied Science and Technology Department, Caliper Life Sciences, Inc., Hopkinton, MA 01748 Karen Heins and Olaf Stelling Agencourt Bioscience Corporation, 100 Cummings Center, Suite 107J, Beverly, MA 01915 ...

Aurum Plasmid 96 Kit Tech Note, Rev A

Neil Duldulao, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Introduction E. coli bacteria have long been used as hosts for plasmid propagation. Several methods have been used for preparing the plasmid, including ...

Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

Audra Day, Colette Schneider, Lisa Ottmers, and Brandt L. Schneider Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA. Introduction Small-scale isolation and purification of plasmi...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

Kathleen A. Maguire-Zeiss, Brandon K. Harv e y, Rita E. Giuliano and H o w a rd J. Federo ff, University of Rochester, Rochester, NY, USA Introduction Transfection efficiency is dependent upon a number of factors, including method of transfection...

SPRINTPREP SINGLE STEP PLASMID PURIFICATION

SprintPrep is an efficient SPRI-based plasmid purification system that can be completed in less than 15 minutes. Unlike most traditional plasmid purification chemistries, SprintPrep isolates plasmid DNA directly from overnight cu...

COSMCPREP HIGH AND LOW COPY PLASMID PURIFICATION

CosMCPrep is a versatile SPRI-based, plasmid purification system that uses a single protocol to purify a variety of high and low copy number template types. This flexible system is easily automated and produces high quality templ...

QIAGEN Plasmid Kits

For purification of up to 10 mg ultrapure plasmid or cosmid DNA Classic QIAGEN Plasmid Kits use gravity-flow anion-exchange tips for efficient purification of plasmid DNA Fe...

DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification

QIAGEN introduces the DirectPrep 96 Miniprep System for cost-effective, fully automated, high-throughput DNA purification from high-copy plasmids. Use of a novel lysis chemistry eliminates the need for a lysate clearing step. P...

QIAfilter Plasmid Kits

For fast purification of up to 10 mg ultrapure plasmid or cosmid DNA Features and benefits Up to 10 mg of ultrapure plasmid DNA Cleared lysates from up to 2.5 liters of LB culture without ce...

QIAGEN Plasmid Kits

For purification of up to 10 mg ultrapure plasmid or cosmid DNA Classic QIAGEN Plasmid Kits use gravity-flow anion-exchange tips for efficient purification of plasmid DNA Fe...

EndoFree Plasmid Kits

For purification of endotoxin-free ultrapure plasmid or cosmid DNA Features and benefits Less than 0.1 EU/g plasmid DNA Yields of up to 10 mg plasmid DNA Greater than 90% recove...

HiSpeed Plasmid Kits

For ultrafast purification of up to 750 g plasmid or cosmid DNA Features and benefits Less than 45 min (Midi) or 60 min (Maxi) from harvested culture to ultrapure DNA Lysate cle...

Efficient Recovery of Ultrapure Plasmid DNA

Nicole Passaretti, Vincent Prezioso, PhD, and Susanne Wagner Nicole Passaretti, Vincent Prezioso, PhDBrinkmann Instruments, Inc., Westbury, NY Susanne WagnerEppendorfNethelerHinz GmbH, Hamburg, Germany ...

Eppendorf Perfectprep Plasmid DNA Isolation Kits

Eppendorf Perfectprep Plasmid DNA Isolation Kits Eppendorf Perfectprep Plasmid DNA Isolation Kits are designed to generate high yields of highly pure plasmid DNA suitable for automated fluorescent sequencing, transfection...

Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit

Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit James Griffith and Kim Vollmer James Griffith, University of Georgia, Department of Genetics, Athens, GA Kimberly Vollmer, Brinkmann Instruments, Biosyte...

RNA-free Plasmid DNA Isolation Protocol

The following protocol is for use with 1 liter cultures grown in LB medium for 1214 hours. It is also used for cultures grown for no longer than 1214 hours in 0.5 liter of enriched growth media such as Terrific Brot...

Basic Plasmid DNA Isolation Protocol

PLG can be used in small and large scale protocols based on alkaline lysis procedure.(1,2,3) The basic protocol given here works with E. coli cultures grown for 1214 hours in up to 500 ml of amplified or non-amplified ...

RNA-free Plasmid DNA Isolation Protocol

The following protocol is for use with 1 liter cultures grown in LB medium for 1214 hours. It is also used for cultures grown for no longer than 1214 hours in 0.5 liter of enriched growth media such as Terrific Brot...

Basic Plasmid DNA Isolation Protocol

PLG can be used in small and large scale protocols based on alkaline lysis procedure.(1,2,3) The basic protocol given here works with E. coli cultures grown for 1214 hours in up to 500 ml of amplified or non-amplified ...

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit Joel Lopez, Melissa Higgins, Chere Griffin and Vince Prezios...

Generate High-Quality, Directional Plasmid cDNA Libraries

Produce larger primary libraries with new plasmid library construction kits Quinn Lu Marie Callahan Tanya Hosfield Barry Neiditch Alan Greener John C. Bauer Mike Kobrin Stratagene Stratagenes pBluescript II, pcmv-script and pAD-GAL4-2.1 XR library construct...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acid purification kits Jeff Braman Scott Basehore Stratagene Cloning Systems, Inc. Stratagene announces a new line of DNA purification products, which provide reliable isolation of high-purity miniprep plasmid,...

High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors

QuikChange XL: a highly versatile kit for site-directed mutagenesis of large plasmids Michael Borns Peter Vaillancourt Holly Hogrefe Jeff Braman Stratagene The QuikChange site-directed mutagenesis kit* characteristically generates mutagenesis efficiencies exceeding 80%...

Efficiently Insert Unique Restriction Sites into Plasmid Vectors

Perform insertion mutagenesis in less than one day Lisa Breister Jeff Braman Stratagene A 28-base insertion was introduced into a plasmid vector using the QuikChange site-directed mutagenesis kit.* The insertion created three unique restriction en...

EGEN, Inc. Announces the Promotion of Jason Fewell, Ph.D., to Vice-President of Preclinical Research and Development

... their novel compositions with nucleic acids and their therapeutic applications. EGEN's research pipeline includes products involving siRNA, shRNA and plasmid DNA for the treatment of various cancers and other diseases and has a number of collaborations with outside investigators, biotech organizations and u...

Frost & Sullivan Recognizes Cisbio Bioassays for Technology Innovation

...capability of one successful reaction during which it covalently links to its substrate. These substrates can be derivatized with HTRF fluorophores. A plasmid construct can be easily generated where the SNAP or CLIP tag is attached to the N-terminus of the GPCR. Thus, a fusion protein comprised of the SNAP/C...

EGEN, Inc. Announces the Promotion of Khursheed Anwer, Ph.D., to President and Chief Scientific Officer

... their novel compositions with nucleic acids and their therapeutic applications. EGEN's research pipeline includes products involving siRNA, shRNA and plasmid DNA for the treatment of various cancers and other diseases and has a number of collaborations with outside investigators, biotech organizations and u...

Clontech Laboratories, Inc. Releases the Xfect(TM) and Xfect(TM) Stem Transfection Reagents

...Stem transfection reagents. These novel transfection reagents are based on biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mammalian cells. For added ease of use, transfections can be carried out entirely in the presence of serum. Xfect shows high efficiency over ...

ImmunoVaccine Technologies Partners with FIT Biotech to advance a therapeutic HIV vaccine

...p with FIT Biotech, a Finland-based, clinical stage company that develops DNA vaccines. This research will formulate FIT Biotech's GTU(R) MultiHIV DNA plasmid with IVT's DepoVax(TM) vaccine delivery system to advance a therapeutic HIV vaccine. "DepoVax(TM) will act as a vector to deliver FIT Biotech's GTU(...

CBDM.T, The Market and Business Intelligence Company Reviews The Vaccine Market

...dead or attenuated organisms, pathogen protein toxin (toxoids), pathogen surface molecules, inactivated or attenuated virus and more recently from DNA plasmid carrying an antigen-coding gene. According to CBDM.T, vaccines represent one of the fastest growing pharmaceutical product categories at over...

GeoVax Labs, Inc. Reports Fourth Quarter and Year-End 2008 Financial Results

... MVA viral vaccine. * In December 2008, GeoVax engaged the services of VGX International, Inc. (VGXI), whereby VGXI will manufacture plasmid (DNA) for use in GeoVax's future preventative and therapeutic clinical human trials. Strengthened Management Team * In February ...

Senesco Technologies Reports Second Quarter Fiscal 2009 Financial Results

...led "Preclinical Studies Using Polyethylenimine (PEI) Nanoparticles Complexed with Eukaryotic Translation Initiation Factor 5A (eIF5A) siRNA and eIF5A plasmid DNA Demonstrates Significant Anti-Myeloma Activity in Vitro and in Vivo". Senesco's preclinical multiple myeloma data was presented at the 20t...

Sangamo BioSciences Announces Results From Diabetic Neuropathy Clinical Trial SB-509-601

...203-1112 and 719-457-0820, respectively. The conference ID number for the replay is 2585543. About SB-509 SB-509 is an injectable formulation of a plasmid encoding a zinc finger DNA-binding protein transcription factor (ZFP TF(TM)) designed to upregulate the expression of the gene encoding vascular endot...

Sangamo BioSciences Reports Third Quarter 2008 Financial Results

...onduction velocity (NCV) in 75% of SB-509 treated subjects compared to 25% of placebo treated subjects. SB-509 is an injectable formulation of plasmid DNA that encodes a ZFP transcription factor (ZFP TF(TM)), designed to upregulate the vascular endothelial growth factor-A (VEGF-A) gene. * O...

Expression Genetics' EGEN-001 Named One of 10 Most Promising Oncology Products by Windhover

...inated solid tumors. The product is based on proprietary TheraPlas(R) delivery technology. It is composed of an interleukin-12 (IL-12) gene expression plasmid and a biocompatible delivery polymer. IL-12 is a potent cytokine which works by multiple mechanisms of action to enhance the body's immune system agai...

Sangamo BioSciences Reports Second Quarter 2008 Financial Results

...ects with mild to moderate diabetic neuropathy over a six month period after a single administration of SB-509. SB-509 is an injectable formulation of plasmid DNA that encodes a ZFP transcription factor (ZFP TF(TM)), designed to upregulate the vascular endothelial growth factor-A (VEGF-A) gene. -- Phase 2 ...

Senesco Signs a Supply Agreement for Polyplus-transfection's Delivery System

...mbination therapy of siRNA against Factor 5A and a plasmid of the Factor 5A gene. Senesco has previously re...al in vivo results using its combination siRNA and plasmid delivered with "in vivo-jetPEI" against subcutaneo...as delivery vehicle for a combination of siRNA and plasmid DNA in this therapeutic approach." About Polyplu...

Rentschler Biotechnologie Announces Cooperation with Boehringer Ingelheim

... proteins, protein scaffolds, monoclonal antibodies, antibody fragments and plasmid DNA. The Biberach site is specialized in highly efficient mammalian cell cu...st with exceptionally high productivities using proprietary systems. In the plasmid DNA manufacturing arena Boehringer Ingelheim in Austria has set the standar...

Genetic Immunity To Present at the Rodman & Renshaw 5th Annual Global Healthcare Conference

...omp is a patented nanoformulation technology that includes disease-specific plasmid DNA encoded antigens. DermaPrep is the topical administration device that d... of diseases. These two components together make up the DermaVir Patch. The plasmid DNA within DermaVir Patch's NanoComp nanoparticles is specific to HIV and t...

Sigma-Aldrich Joins Phase 2 of The RNAi Consortium to Validate shRNA Libraries

...and mouse genomes. Sigma-Aldrich's MISSION(R) shRNA portfolio is the exclusive source for researchers to access TRC and TRCII shRNA libraries in the plasmid DNA and lentiviral formats. All of the shRNA reagents will be fully functionally validated during phase 2 of The RNAi Consortium. This validation data...
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