Pipet in Biological Technology

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

...s MegaBACE 1000) Materials: v 96-well PCR microplates (MJ Research Hard-Shell Thin Wall P/N HSP-9601) Tempo-100 pipet Tips (P/N 66670) Tempo 25 μL disposable tips (P/N 104035) pUC19 DNA control template (United States Biochemicals P/N 76632...

Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

...ettor in which channels C and G had slightly loose pipet tips. The wells in these channels clearly have sli...e an Outcome column in the Group Table to flag any pipet channel falling outside the acceptable range. To d... + 3% and a precision requirement of + 1.5% for pipet dispense volumes of 1 In this application no...

Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)

...nts Note: In some cases, you may want to use stored pre-read values (see below). Step 1 Select a clean microplate and pipet distilled water into each well in approximately the same volumes as the intended samples. Accurate pipetting is not necessary. Step 2 Put...

Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)

...The diluted samples should contain 1 - 25 g Pi/mL. pipet 2.5 mL of each into 12 x 75 mm glass tubes. Step 4...24 hours. Step 6 Test tube/cuvette method: pipet 1.0 mL aliquots of each sample and standard into 1...or for at least one hour. Microplate method: pipet 150 L aliquots of each sample, standard and blank ...

PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

...hlength correction. In some cases, you may choose to use stored pre-read values (See below). Step 1 Select a clean microplate and pipet distilled water into the wells in approximately the same volumes as the intended samples. Accurate pipetting is not necessary. ...

Direct Counting of Millipore MultiScreen Filtration Plates.

... (Optifluor, Packard Instrument Company). The second plate was processed by adding 20 mL MicroScint-O to each well using a semi-automatic multichannel pipet and counting on TopCount. Stimulation curves obtained with the TopCount and with conventional LSC are shown in Figure ...

A simple method to sequence from bacterial colonies using [a-33P] radiolabeled ddNTPs and Thermo Sequenase

...ube. 2. Carefully swirl the colony in water with a sterile 200ml pipet tip. pipet up and down several times until the colony is fully resuspended. ...

Modification of Bio-Rad DC Protein Assay for Use With Thiols

...ate may form after 1 day. If a precipitate forms, vortex. Do not pipet the undissolved precipitate, as this will likely plug the tip of...hould be prepared in the same buffer as the sample. 4. pipet 100 l of standards and samples into clean, dry test tubes. 5. For...

Real-Time PCR: General Considerations, Rev A

...fic amplification during preparation Make up a master mix with sufficient volume to prepare all replicate samples pipet once per well DNA Source The source of the template affects the accessibility of the target sequence an...

Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus

... least 30 min. Store purified RNA sample at 70 to 80C until use. 3. Before use, vortex sample vigorously for 5 sec and pulsespin. pipet sample up and down several times to ensure adequate mixing. back to top ...

Total RNA Isolation From 50100 mg Plant Tissue

... tube. Store purified RNA sample at - 7 0 C t o -80C until use. 3. Before use, vortex sample vigorously for 5 sec and pulsespin. pipet sample up and down several times to ensure adequate mixing. back to top ...

Total RNA Isolation From 500 l Body Fluid

...ternatively, store purified RNA sample at -70 to -80C until use. 3. Before use, vortex sample vigorously for 5 sec and pulsespin. pipet sample up and down several times to mix the sample further. back to top ...

InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C

... stirrer to maintain the matrix in suspension. The pipet tip used should have a large bore, such as on a 1,000 l pipet tip (Bio-Rads TBR tips, catalog #223-93... stirrer to maintain the matrix in suspension. The pipet tip used should have a large bore, such...

Genomic DNA Isolation From 1 ml of Body Fluid

...erile 15 ml centrifuge tube containing 5 ml cell lysis solution. pipet up and down several times to mix thoroughly. Note: if the sample...l y, if p a rticulates are present, transfer supernatant using a pipet so that particulates are excluded. If the DNA yield is expected ...

Genomic DNA Isolation Protocol for 40 l Avian Whole Blood

... DNA isolation results. 2. Add 40 l whole blood to a 15 ml microfuge tube containing 6 ml cell lysis solution. Quickly pipet up and down 35 times to lyse the cells. Usually no incubation is required; however, if cell clumps are visible after mixing, inc...

Genomic DNA Preparation from RNAlater Preserved Tissues

...e to a new 2 ml tube using a 1 ml pipet tip with the end cut off. The wid...A should spool. Remove DNA with a pipet tip to a fresh tube. Wash DNA in 70% ethanol by in... DNA SHOULD NOT BE RESUSPENDED BY pipet OR VORTEX, AS IT WILL SHEAR!!! ...

Multichannel Pipetting: How to Choose the Correct Pipettor

...ing force is required (Figure 2). The spring mechanism allows the user to pipet with a constant and very low force, regardless of the volume, which has a...inute piston movements (small volumes such as 0.5 L), the easier it is to pipet accurately. Due to the instrument's design, the maximum pipetting for...

Uses and Applications of FuGENE 6 Transfection Reagent

... Check the stock DNA concentration, and determine how much you will need for 1 and 2 g. If the stock is 2 g/l, then decide if you can accurately pipet 0.5 l. It might be better to dilute the DNA using procedures that give accurate dilutions. If you need to dilute the DNA, use sterile DNase-free...

Transfection Reagent Selector Kit Handbook

...e tube. pipet 100 l of mastermix A into tubes 1, 2, 3, each of w... will finally contain 0.2 g of DNA. pipet 100 l of mastermix B into tubes 4, 5, 6, each of w... will finally contain 0.4 g of DNA. pipet 100 l of mastermix C into tubes 7, 8, 9, each of w...

MinElute PCR Purification Kit

...r removal 80% recovery of fragments (70 bp 4 kb) 5 g DNA binding capacity No loose resins or slurries to pipet Efficient Primer Removal Using the MinElute PCR Purification Kit Analysis of PCR products ...

PolyFect Transfection Reagent

...ect Reagent to the DNA solution, mix, incubate for 510 minutes, add growth medium (which can contain serum and antibiotics), and pipet the PolyFectDNA complexes onto the cells. The cells are then incubated for gene expression. No post-transfection removal of comp...

HotStarTaq PCR Handbook

...duct or a smear, exchange all reagents. Use disposable pipet tips containing hydrophobic filters to minimize cross-... Use a separate set of pipets for the PCR master mix. Use of pipet tips with hydrophobic filters is strongly recommended. ...

The QIAGEN Guide to Animal Cell Culture

... disinfectant. 5. Slowly pipet the thawed cell suspension into the cell culture ...ensitive cell types. For such cell types, pipet the thawed cell suspension into a sterile centrifu...s trypsin activity. 7. Gently pipet the cells up and down in a syringe with a needle ...

The QIAGEN Guide to Animal Cell Culture

...excessive force to expel material from pipets; and do not bubble air through liquids with a pipet. Avoid releasing the contents of a pipet from a height into the receiving vessel. Expel liquids as close as possible to the level of liquid of the receiving vessel, or allow the ...

Ergonomics and Liquid Handling How can BrinkmannTM Help ?

... October 1995:1011 . Haskell, Natalie Kuhtmann. Ergonomic considerations in avoiding cumulative trauma disorder from pipet use, American Laboratory , January 1998:14. Friedriksson, Kerstin, Laboratory work with automatic pipettes: a st...

Total RNA Isolation Protocol 1,2,3

...Homogenize washed, pelleted cells by pipetting the mixture up and down until the pellet is fully suspended. Use a small bore pipet to collect the cell homogenate. Transfer all of the homogenate to a pre-spun (12,00016,000 x g for 12 minutes) P...

RNA-free Plasmid DNA Isolation Protocol

... 5 minutes at 4C. Resuspend bacterial pellet in a total of 30 ml 25 mM Tris-Cl/10 mM EDTA (pH 8.0). pipet up and down or vortex as necessary to fully resuspend the bacteria. Add 30 ml room temperature 0.2 N NaOH/1.0% S...

Mouse Tail Genomic DNA Isolation Protocol(1)

... Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA. Place a 1 cm tail sample ...

Genomic DNA Isolation Protocol(1,2,3)

... by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic ... the side of the tube. Place a Pasteur pipet tip at the interface of the DNA-Ethanol solution and spool the DNA onto the pipet tip by swirling the pipet, keeping th...

Basic Plasmid DNA Isolation Protocol

...room temperature. Resuspend bacterial pellet in a total of 15 ml 25 mM Tris-Cl/10 mM EDTA (pH 8.0). pipet up and down or vortex as necessary to fully resuspend the bacteria. Add 15 ml room temperature 0.2 N NaOH/1.0% S...

RNA-free Plasmid DNA Isolation Protocol

... 5 minutes at 4C. Resuspend bacterial pellet in a total of 30 ml 25 mM Tris-Cl/10 mM EDTA (pH 8.0). pipet up and down or vortex as necessary to fully resuspend the bacteria. Add 30 ml room temperature 0.2 N NaOH/1.0% S...

Basic Plasmid DNA Isolation Protocol

...room temperature. Resuspend bacterial pellet in a total of 15 ml 25 mM Tris-Cl/10 mM EDTA (pH 8.0). pipet up and down or vortex as necessary to fully resuspend the bacteria. Add 15 ml room temperature 0.2 N NaOH/1.0% S...

Genomic DNA Isolation Protocol(1,2,3)

... by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic ... the side of the tube. Place a Pasteur pipet tip at the interface of the DNA-Ethanol solution and spool the DNA onto the pipet tip by swirling the pipet, keeping th...

Mouse Tail Genomic DNA Isolation Protocol(1)

... Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA. Place a 1 cm tail sample ...

Identification of Differentially Expressed Gene Products with the CastAway System*

...ions at the positions corresponding to the band of interest. The pipet tip containing the aspirated gel section was placed into a micro... and 0.1 mM EDTA and was incubated at 65 C for 2 hours. The pipet tip was removed, and the tube was centrifuged for 5 to 10 seconds ...