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Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

Evelyn McGown, Ph.D. and Anna Lam, B.S. Molecular Devices Corporation, 8/99 Modern assays for proteolytic activity typically use synthetic chromogenic or flu-orogenic peptide substrates. Enzyme selectivity is obtained by choosing a peptide sequence uniquely recognized by the catalytic site of a particular enzy...

Off-the-grid networking can help you cut past chit-chat

When Midwest tech people talked about networking a decade ago, they were mostly talking about reaching out to their friends and colleagues for job opportunities and business development leads. In other words, to most Midwest tech networkers, "networking" meant "using your existing net...

SINGLE PASS SEQUENCING

Agencourt offers a single pass sequencing service for high copy number plasmids and PCR amplicons. This service is fueled by our high performance sequencing pipeline, which processes over 20 million Phred20 bases per day. The key benefits we offer our customers are: Long Phred20 read lengths...

Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer canbe used to analyze apoptotic cell samples. Performance of two assaysfor apoptosis detection is demonstrated and the data are compared tothose obtained with a conventional flow cytometer. Histogram quality...

Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays

The understanding of the cellular signaling processes leading to programmed cell death (apoptosis) is of utmost importance in the study of autoimmune diseases such as rheumatoid arthritis or Parkinsons and Alzheimers disease. Here, errors in the signaling cascade are believed to lead to premature apoptosis in the affected t...

P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor

Introduction This Application Report is part of a series documentingculture growth in the BioFlo 110. With appropriatevessels and control modules, the BioFlo 110 can efficientlygrow yeast and bacteria, as well as mammalian,plant cells and insect cells. Pichia pastoris:...

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

Over the last several decades, geneticists have learned how to manipulate DNA to identify,excise, move and place genes into a variety of organisms that are quite different genetically fromthe source organism. A major use for many of these recombinant organisms is to produceprot...

A Midwest passage to India, Part II

When I last left you, it was in the middle of a week's trip to India as part of an Illinois trade mission to open up a new trade office in that country. The initial part of the trip took me to Delhi (both New and Old), the ancient capitol of Agra (home to the Taj Mahal among other things). We then moved on to Mumbai (Bombay) for the last part of the trip. I promised...

House passes bill to lift stem cell funding restrictions

But the vote of 253 to 174 is not enough to override a promised pr...

Will wireless Internet bypass the central city?

The to build a citywide wireless Internet network. Taxpayers are off the hook bec...

New Silicon Pastures director envisions more home cooking for Wisconsin angels

While others are talking about brain drain and t...

NimbleGen names CFO with capital-raising past

David Snyder brings more than 15 years of experience in capital raising, finance, and general management in a range of industries, including t...

Doyle urges passage of federal stem cell bill

Doyle, traveling to Cottage Grove, Eau Claire, and Green Bay, ca...

QBI's PreserveX product passes initial test

Kauten, CEO of , recently received good news from an initial screening of its PreserveX Polymeric Micelles, a core product. The screening, which employed UGT1A1, an enzyme that metab...

Winners of past business plan contests secure funding, credibility

Several emerging technology companies have earned much-needed start-up momentum, and attracted the attention of investors, after submitting winning business plans to a competition that has attracted hundreds of ideas. The Governor's Busine...

EraGen diagnostic passes UW tests, could quickly detect bird flu

The MultiCode panel technology, which is being developed by , a Madison...

Fiserv to partner with PassMark Security

The move comes as financial institutions are under obligation, by the end of 2006, to comply with Fede...

Tom Koulopoulos shares passion for IT's role in business

"W...

Passports to contain RFID chips next year

International travelers will find a new accessory among their standard travel gear starting in late 2006: an RFID-equipped passport. The new passports, mandated by State Department regulations, will feature a 64 KB chip containing a duplicate of the information featured on the passport's physical page, which the government hopes will strengthen national security. "The electronic...

Cloning ban passes Assembly, Doyle pledges veto

The state Assembly on Thursday passed AB 499, a proposed ban on all forms of human cloning in Wisconsin. The bill would outlaw the cloning of embryos both for reproductive purposes and for what practitioners have called research or therapeutic cloning, the making of a cloned embryo for the purpose of extracting stem cells. Penalties for violating the bill if it were to become law would g...

Kanavas thinks personal ID theft bill will pass this year

State Senator Ted Kanavas remains optimistic that his bill requiring notification of electronic data breaches will pass the legislature yet this year. Senate Bill 164, co-authored by Kanavas, R-Brookfield, and Representative Jeff Fitzgerald, R-Horicon, would require companies that keep personal data on customers and clients to notify those people when that data is obtained by unauthoriz...

As HIPAA deadline passes even the unprepared are safe for now

Unlike the This makes sense to many in the field; the HIPAA rule involves too many software protocols throughout the healthcare...

Information technology past the end of the Earth

So it's 1492. At least that's how we're acting. The grand majesty of the IT industry is huddled in our comfortable little corner of the world. Yeah, we send the occasional skirmish over into the Kingdom of Marketing or Manufacturing or wherever we think "the business" is, but our maps of the world mostly contain a great deal of detail in the familiar territories of...

Wisconsin cannot afford to take a pass on Doyles biotech plan

The response has been less than overwhelming to Governor Jim Doyles proposal to invest nearly $750 million in public and private money in Wisconsins biotechnology future. Budget-conscious members of the Legislature have choked on the price tag, opponents of stem-cell research have once again protested, and Milwaukee politicians have asked if theyre being shortchanged in favor of Madison....

State faces revenue drop as federal Internet tax ban passes House

Starting November 1, 2006, Wisconsin will no longer be allowed to tax Internet usage, in a move that would open up savings for users but could put a hole in the state budget. The federal measure to eliminate the tax was authored by , R-Wis., who said home users could save around a dollar a...

Congress passes bill to ease researchers collaboration worries

The U.S. House of Representatives has passed a bill that allows researchers to work together without worrying that their collaboration will be used against them in a patent dispute. After a 1997 court decision, work shared between different institutions could count as prior art, said Andy Cohn, spokesperson for the Th...

Irish flute passion drives e-commerce spirit for Eaton techie

During the day, Dr. Mark Polczynski is the technology consultant to the vice president of engineering at Polczynski's two-person business, is not a huge money maker. "My take out of this is very soft," he said. But the t...

Tech Council passes E-Learning efforts for Northern Wisconsin

ASHLAND, Wis. In a bid to spark creation of technology-based businesses and jobs in a largely rural part of the state, the A resolution supporting creation of a task force to push for the c...

The passing of Ronald Reagan, and other Americans, is a reminder of the promise of stem-cell research

MADISON My fathers slow retreat in the face of Alzheimers Disease was typical of how it robs people of their lives. His degeneration was slow, at first, but gathered momentum over time. Independence turned to dependence. At the end, Claude Richmond Still was in otherwise fine physical shape for a man of 80, but his memory and even his ability to mumble more than a cogent word or two was gone. Hi...

MyWeather-powered alerting system surpasses 165,000 downloads

MADISON - Powered by MyWeather t...

Wisconsin Assembly passes capital investment bill, revised CAPCO program not presented

MADISON - The Wisconsin Assembly passed a bill Friday designed to promote capital investment and technological entrepreneurship by means of tax credits, grants and loans. The legislation, , was only one of two venture capital-oriented bills co-authored by Sen. Ted Kanavas, R-Brookfield, to hit the Assembly...

Senate Passes an Investment Capital Package; the Assembly Should Follow Suit

MADISON State Sen. Ted Kanavas was still beaming on Saturday, a day after the Legislatures upper house passed bills that could close Wisconsins venture capital gap and build a stronger in-state network for entrepreneurs Not that we havent dealt with some pretty big issues this fall, but this is the most important so far to the states economy, said Kanavas, a Brookfield Republican who wo...