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POL in Biological Technology

PolyOne Corporation Announces an Exclusive License Agreement with Battelle for Bio-Plasticizer Technology

... AVON LAKE, Ohio, Aug. 5 /PRNewswire-FirstCall/ -- PolyOne Corporation (NYSE: pol ), a premier provider of specialized polymer materials, services and solutions, announced today that it has entered into an exclusive license arrangem...

Monsanto Company Signs Share Subscription Agreement With Devgen

...ive Officer of Devgen. "This strengthens Devgen's ability to develop and commercialize innovative biotech traits and seeds that meet farmers' needs." pol Bamelis, Chairman of Devgen, added: "We are pleased to strengthen our relationship with Monsanto. I would like to take the opportunity to acknowledge ...

RNA Interference and Gene Silencing: History and Overview

...NA expression vector developed by a different research group encodes the sense and antisense siRNA strands under control of separate pol III promoters (52 ). The siRNA strands from this vector, like the shRNAs of the other vectors, have 5 thymidi...

siRNA Expression Vectors with Selectable Markers

...erted repeat is complementary to the mRNA to which the siRNA is targeted. A string of thymidines added to the 3' end serves as a pol III transcription termination site. (See Design of Vector Encoded siRNAs in sidebar). Once inside the cell, the vector constit...

Selecting siRNA Sequences to Incorporate into the pSilencer Vectors

...pears to be comparable for both in vitro prepared siRNAs and RNA pol III-expressed siRNAs. This is also true for chemically synthesized siRNAs versus RNA pol III-expressed siRNAs. Therefore, it is not necessary to re-scree...

More siRNA Vectors for RNA Interference

...e control of the U6 promoter and several other RNA pol III promoters, it was found that RN...udies have shown that different RNA pol III promoters generate small RNAs that ...tract to terminate transcription by RNA pol III. The insert design shown above is s...

Get the 5'-End

...estinal Phosphatase (CIP) to remove the 5'-phosphate from all RNA species except those that have a cap structure (present on all pol II transcripts). Molecules that are dephosphorylated in the CIP reaction include rRNA, tRNA, and most importantly, fragmented mR...

Drive Long-Term Silencing with CMV Vectors

...and performs better than most pol III promoters under long term ... clones is possible interference between the pol II and III promoters. Althoug... it is hypothesized that when pol II and III promoters are in c...

Five Ways to Produce siRNAs

... in mammalian cells (14). RNA pol III was chosen to drive siRNA ...iRNA Plasmid Expression Vectors feature U6 and H1 pol III promoters, an ampicillin ... and colleagues (5), SECs include an RNA pol III promoter, a sequence encoding an si...

GENE SILENCING WITH PCR PRODUCTS

... to a hairpin siRNA template and an RNA pol III transcription termination site. When transfect... cells, the SECs are transcribed by endogenous RNA pol III to generate a small hairpin siRNA w... one or more PCRs with the included RNA pol III promoter-containing DNA t...

siRNA Design Guidelines

...s also compatible with using RNA pol III to transcribe hairpin siRNAs because RNA pol III terminates transcription at 4-6 nucleotide ... as a termination signal for RNA pol III, avoid stretches of > 4 T's or A's ...

High-Titer Retroviral Vectors for Gene Delivery

... for viral gag, pol, and env proteins, all of which are required in trans to make virus. 2 The gag gene encodes internal structural proteins; the pol gene encodes reverse transcriptase (RT) and integrase; and the env gene encodes the viral envelope protein, which resides on the viral surface and...

Safe Production of High-Titer Retrovirus

...ssettes for viral gag, pol, and env proteins, all of which are required in trans to make virus. The gag gene encodes internal structural proteins, pol encodes reverse transcriptase (RT) and integrase, and the env gene encodes the viral envelope protein. The envelope protein resides on the viral s...
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(Date:7/23/2014)... , July 23, 2014 ... threat detection, announced today it has been named in ... The Market Guide replaces Gartner,s well-known Magic Quadrant for ... categorized BioCatch as one of several vendors to deliver ... analysis will become a standard feature of at least ...
(Date:7/23/2014)... potential for clinical use of induced pluripotent stem ... previously been hindered by the risk of dysregulated ... ability to use etoposide treatment to halt teratoma ... disease, specifically acute myocardial infarction, is demonstrated in ... , a peer-reviewed journal from Mary Ann Liebert, ...
(Date:7/23/2014)... the International Journal of Fuzzy Systems ( ... Systems Association (TFSA). The cooperation will allow the journal ... fuzzy research., The International Journal of Fuzzy Systems ... with the theory, design and application of fuzzy systems, ... ranging from hardware to software. Launched in 1999, the ...
Breaking Biology News(10 mins):BioCatch Featured in Gartner's 2014 Online Fraud Detection Market Guide 2BioCatch Featured in Gartner's 2014 Online Fraud Detection Market Guide 3
(Date:7/23/2014)... if a suspicious skin lesion is painful or itchy ... to be cancerous, according to a new study headed ... Dermatology at Temple University School of Medicine. , The ... July 23, 2014, found that nearly 36. 9 percent ... 28.2 percent involve pain. Non-melanoma skin cancers specifically, ...
(Date:7/23/2014)... AB (PRWEB) July 23, 2014 ... of in-home care for seniors, is proud to announce ... Series featuring experts in a wide range of health ... clinical social worker and award-winning author Nancy Kriseman, and ... and ways to prevent burnout by becoming more self-aware. ...
(Date:7/23/2014)... Regularly checking the pulse of a stroke survivor may ... is the method of choice for checking for irregular ... had a stroke. Our study shows it may be ... people who might need more thorough monitoring to prevent ... of Erlangen University in Germany. The study ...
(Date:7/23/2014)... WHAT: The National Institute of Biomedical Imaging and ... Investigator Symposium on July 30, 2014 on the NIH ... new investigators covering a wide breadth of NIBIB-funded research. ... Adam Cohen, Harvard, Engineering Microbial Rhodopsins as Optical Voltage ... Testing Fluorescently Labeled Probes for Nerve ...
(Date:7/23/2014)... July 23, 2014 Global Cardio Care, ... Enhanced External Counterpulsation (EECP), is celebrating the 100th ... therapy at the health center. , Weber has ... Since receiving ongoing EECP therapy since 1999, he no ... Soulati, CEO of Global Cardio Care, introduced the ...
Breaking Medicine News(10 mins):Health News:Research led by Temple's chair of dermatology: Pain and itch may be signs of skin cancer 2Health News:Home Care Assistance Calgary to Host Public Webinar on the Topic of Caregiver Mindfulness 2Health News:Home Care Assistance Calgary to Host Public Webinar on the Topic of Caregiver Mindfulness 3Health News:Routine Pulse Check May Prevent Second Stroke, Study Says 2Health News:Global Cardio Care Celebrates EECP Patient’s 100th Birthday 2
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