POL in Biological Technology

PolyOne Corporation Announces an Exclusive License Agreement with Battelle for Bio-Plasticizer Technology

... AVON LAKE, Ohio, Aug. 5 /PRNewswire-FirstCall/ -- PolyOne Corporation (NYSE: pol ), a premier provider of specialized polymer materials, services and solutions, announced today that it has entered into an exclusive license arrangem...

Monsanto Company Signs Share Subscription Agreement With Devgen

...ive Officer of Devgen. "This strengthens Devgen's ability to develop and commercialize innovative biotech traits and seeds that meet farmers' needs." pol Bamelis, Chairman of Devgen, added: "We are pleased to strengthen our relationship with Monsanto. I would like to take the opportunity to acknowledge ...

RNA Interference and Gene Silencing: History and Overview

...NA expression vector developed by a different research group encodes the sense and antisense siRNA strands under control of separate pol III promoters (52 ). The siRNA strands from this vector, like the shRNAs of the other vectors, have 5 thymidi...

siRNA Expression Vectors with Selectable Markers

...erted repeat is complementary to the mRNA to which the siRNA is targeted. A string of thymidines added to the 3' end serves as a pol III transcription termination site. (See Design of Vector Encoded siRNAs in sidebar). Once inside the cell, the vector constit...

Selecting siRNA Sequences to Incorporate into the pSilencer Vectors

...pears to be comparable for both in vitro prepared siRNAs and RNA pol III-expressed siRNAs. This is also true for chemically synthesized siRNAs versus RNA pol III-expressed siRNAs. Therefore, it is not necessary to re-scree...

More siRNA Vectors for RNA Interference

...e control of the U6 promoter and several other RNA pol III promoters, it was found that RN...udies have shown that different RNA pol III promoters generate small RNAs that ...tract to terminate transcription by RNA pol III. The insert design shown above is s...

Get the 5'-End

...estinal Phosphatase (CIP) to remove the 5'-phosphate from all RNA species except those that have a cap structure (present on all pol II transcripts). Molecules that are dephosphorylated in the CIP reaction include rRNA, tRNA, and most importantly, fragmented mR...

Drive Long-Term Silencing with CMV Vectors

...and performs better than most pol III promoters under long term ... clones is possible interference between the pol II and III promoters. Althoug... it is hypothesized that when pol II and III promoters are in c...

Five Ways to Produce siRNAs

... in mammalian cells (14). RNA pol III was chosen to drive siRNA ...iRNA Plasmid Expression Vectors feature U6 and H1 pol III promoters, an ampicillin ... and colleagues (5), SECs include an RNA pol III promoter, a sequence encoding an si...

GENE SILENCING WITH PCR PRODUCTS

... to a hairpin siRNA template and an RNA pol III transcription termination site. When transfect... cells, the SECs are transcribed by endogenous RNA pol III to generate a small hairpin siRNA w... one or more PCRs with the included RNA pol III promoter-containing DNA t...

siRNA Design Guidelines

...s also compatible with using RNA pol III to transcribe hairpin siRNAs because RNA pol III terminates transcription at 4-6 nucleotide ... as a termination signal for RNA pol III, avoid stretches of > 4 T's or A's ...

High-Titer Retroviral Vectors for Gene Delivery

... for viral gag, pol, and env proteins, all of which are required in trans to make virus. 2 The gag gene encodes internal structural proteins; the pol gene encodes reverse transcriptase (RT) and integrase; and the env gene encodes the viral envelope protein, which resides on the viral surface and...

Safe Production of High-Titer Retrovirus

...ssettes for viral gag, pol, and env proteins, all of which are required in trans to make virus. The gag gene encodes internal structural proteins, pol encodes reverse transcriptase (RT) and integrase, and the env gene encodes the viral envelope protein. The envelope protein resides on the viral s...