Oti

Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits

Allelic discrimination kits with excellent specificity, sensitivity, andassay throughput Stratagene Because of their unique hairpin conformation, molecular beacon probes canrecognize single base differences, making them ideal for mutation detection andallelic discrimination. Base...

Genotyping Single Nucleotide Polymorphisms with Molecular Beacons

Stratagenes custom molecular beacon synthesis assists in detecting SNPsquickly and easily University of Colorado Health Sciences Center, Denver, Colorado to detect a single nucleotide polymorphism (SNP) in a region of c...

Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells

<A href="http://www.biocompare.com/techart.asp?id=575">Actinobacillus pleuropnomoniae <A href="http://www.biocompare.com/techart.asp?id=576">Actinomyces viscosus <A...

Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells

<A href="http://www.biocompare.com/techart.asp?id=575">Actinobacillus pleuropnomoniae <A href="http://www.biocompare.com/techart.asp?id=576">Actinomyces viscosus <A...

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Sonja Vorwerk, Max Planck Institut fr Zchtungsforschung, Carl-von-Linn Weg 10, 50829 Cologne, Germany Phone: ++49-221-5062-441, Fax. ++49-221-5062-413, e-mai...

PCR Grade Deoxynucleotides

Highly purified deoxynucleotides for optimal results and maximum sensitivity in all PCR and RT-PCR applications Unsurpassed purity and performance with PCR Grade Nucleotides! Produce sensitive and consistent results with the newly developed PCR grade deoxy-nucleotides from RAS. Improve performance and sensitivity...

Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA

One of the RNA silencing products available from Ambion is a kit for the synthesis of siRNA, the <A href="http://www.ambion.com/catalog/CatNum.php?1620">Silencer <A href="http://www.ambion.com/catalog/CatNum.php?16000"> siRNA Construction Kit . While this kit is very cost effective...

Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.

Recent advances in combinatorial chemistry and genomics programs have led to an unprecedented increase in both the number of compounds and potential drug targets that can be screened. The development of automated liquid h...

A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)

β -lactamases are either plasmid or chromosomally encoded bacterial enzymes which hydrolyze β -lactam antibiotics. The majority of β -lactamases produced by clinical isolates are serine active site enzymes. One successful approach to combating the serine active site β -lactamases is the use of β -lactamase inhibit...

Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

By Yan Zhang., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the OliGreen Pro software from...

Ready to leave? Why you shouldn't give two weeks' notice

The "Exit Stage Right" article on Why? Because giving two week's notice (let alone four week's notice) went out with nickel beers. The article also needs a reality check on some of its observations and assertions of how life is in today's corporate...

Trouble-shooting: Low yield

Here are some troubleshooting hints that we have gathered regarding low yield after PCR reactions: <a href="#Option1">Primer annealing temperature is too high...

Trouble-shooting: Non-specific bands

Here are some troubleshooting hints that we have gathered regarding non-specific bands after PCR reactions: <a href="#Option1">Non-optimal Mg<s...

Trouble-shooting: Misincorporation or low fidelity

Here are some troubleshooting hints that we have gathered regarding misincorporation or low fidelity of PCR reactions: <a href="#Option1">Magne...

Trouble-shooting: No product

Here are some troubleshooting hints that we have gathered if your PCR yields no product: Non-optimal Mg...

Trouble-shooting: Smeared bands

Here are some troubleshooting hints that we have gathered regarding smeared bands after PCR reactions: <a href="#Option4">Primer annealing temperature is too low...

PCR Grade Deoxynucleotides Additional power to perfect your PCR experiments

Deoxynucleotides are vital components in amplification reactions, and the purityof these reagents significantly influences the results of your experiments. Roche Applied Science developed a new manufacturing and purificationprocess for...

PCR-Grade Nucleotides Larger Pack Sizes Now Available

Roche Applied Science now offers new larger pack sizesof PCR-Grade Nucleotides. These larger pack sizescombine the best available quality with attractive pricing. Single dN...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

90% of mammalian RNA from complex mixtures of host-bacterial samples Seamless integration with MICROB <...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

90% of mammalian RNA from complex mixtures of host-bacterial samples Seamless integration with MICROB <...

Synthesize High Yields of Biotinylated aRNA

Ambion now offers an improved MessageAmp II Kit with biotin-11-UTP. MessageAmp II-Biotin Enhanced provides a complete single round amplification kit for biotin array analysis. The biotin modified UTP and ratio of labeled...

Oligonucleotide Purity Analysis by Capillary Electrophoresis

Introduction Millions of oligonucleotides are synthesized each year for use as primers or probes in genetic analysis. Quality control of these preparations has traditionally been done by slab gel electrophoresis or HPLC, but these methods may require expensive reagents and laborious protocols, and may...

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

Contributed by John M. Sedivy, Department of Molecular Biophysics andBiochemistry, Yale University, 333 Cedar Street, New Haven, CT 06510. Genes of higher eukaryotes can be hundreds of kilobases in size. The need to rapidly clone and manipulate DNA molecules in that range has become acute in co...

Examples of Electro-Transfected Eukaryotic and Prokaryotic Cells

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Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor

M. Adams and R. Deadman GE Healthcare, Piscataway, NJ, US GE Healthcare offers a range of DNA amplification products to meet all genomic DNA and sequencing template preparation needs. A combination of these kits with the Genesis Freedom™ Robotic Sample Processor (RSP) from Tecan (Fig 1) creates a total solution for high-throu...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

Macrophage foam cells are integral in the development of atherosclerotic lesions, however gene expression studies are complicated by the cellular heterogeneity of atherosclerotic plaque. This application note describes a protocol for LCM of cells identified immunohistochemi...

High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS

The animal meat samples were prepared by homogenization, organic solvent washes, hydrolysis and derivatization follo...

USB OptiKinase Eliminates Base Bias in Labeling of Oligonucleotide 5'-Ends

. Historically, T4 PNK has played a crucial role in molecular biology and it continues to be important in...

Femtogram Sensitivity of the Antipsychotic Drugs Raclopride and Ritanserin Using a Varian 1200L LC/MS/MS

Varian, Inc. These two compounds were used to demonstrate low-level sensitivity of...

Getting noticed in Genetown: Wisconsin biotech on display

That's not the case in...

Two-week notices: People are still concerned

Some don't even know who the...

NASDAQ puts Merge on notice again

Representatives of Merg...

SGI receives court approval for bankruptcy motions

The approval came during a hearing in which the U.S. Bankruptcy Court for the Southern District of New York granted approval o...

Notion of bandwidth spedometers for DSL, cable sparks controversy

Is the advertised high-speed Internet access you're paying for actually what you're receiving? Two weeks ago, the simple issue of whether DSL and high-speed cable providers should supply bandwidth speedometers to customers got many people talking nationwide, writes adjunct Northwestern professor James Carlini. Since this topic was suggested, quite a bit of feedback rolled in on a DSL Rep...

Employees debate ethics of giving two weeks' notice

About a year ago, I wrote a column criticizing an "advice article" in the Chicago Tribune about the practice of giving two-week notices when leaving a company. ( ) It was published both on ePrairie and with the Wisconsin Technology Network (WTN). The Wisconsin copy was left...

Oshkosh robotic truck returns to desert

The first driverless vehicle that Now the company is getting ready to return to the field with the second version of "Ter...

Voting machine source code must be available for review

Correction: the original story was based on an official summary that referred to the bill as introduced and did not take into account certain amendments. Under the law as enacted, the source code would be placed into escrow with the elections board. It would be analyzed in the case of a recount, but not open to the public. WTN very much regrets the error and any confusion it may have caused.</...

Group promoting classified research elects officers

The Wisconsin Security Research Consortium held an organizational meeting last week to name its leadership. The group will try to bring classified research to Wisconsin institutions. Tom Hefty, former co-chairman of the Governor's Economic Growth Council, is president of the board of directors. He is now with law firm Reinhart, Boerner and Van Deuren. <a href="http://www.engr.wi...

Oshkosh's robotic truck qualifies for DARPA challenge

and the University of Parma, Italy, announced that their robotic truck, TerraMax, qualified late Wednesday to compete in the $2 million DARPA Grand Challenge, a 150-mile race of unmanned vehicles across the Mojave Desert on Sat...

KUKA Robotics set to unveil Appleton training center

US headquarters in Clinton Township, Mich. <...