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Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library

Department of Cell Biology, Clevela...

Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells

Stratagene mammalian expressionsystem allows tightc...

High-Throughput Cultivation of Bacterial Clones

Stratagene The tempest thermoshaker and a 96-deep-well round-bottom plate were used to cultivate bacterial clones in a...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

Epitope Tagging in E. coli Katherine Felts Denise Wyborski John Bauer PeterVaillancourt Stratagene LIC cloning kit...

Yeast Protein Production System Features High Yields and One-Step Purification

The ESP system provides high-yield protein production in a eukaryoticorganism Quinn Lu * Bruce Jerpseth * Tim Sanchez * John C. Bauer * AlanGreener Stratagene Cloning Systems, Inc. provides novel opportunities for rapid, inexpensive and high-yield p...

pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins

Faster, easier screening for difficult transfections when using thecomplete control kit Stratagene We describe the vector pFB-ERV, an MMLV-based replication-defectiveretroviral vector for delivery of the ecdysone receptor proteins RXR and VgEcR.In stable cell lines infected with an estima...

Mammalian and Bacterial Expression in One Vector

Novel pdual expression vector for high-level expression in bothmammalian and bacterial cells Stratagene Stratagene has developed the pdual expression vector,* which permitshigh-level expression of heterologous genes in both eukaryotic and prokaryoticsystems. The vector contains the promoter an...

Salmonella typhimurium

Salmonella typhimurium LT2...

Salmonella typhimurium

Salmonella typhimurium LT2...

C. therm. Polymerase One-Step RT-PCR System

for one-step RT-PCR amplification of up to 3 kb RNA targets Cat. No. 2 016 338 50 reactions Cat. No. 2 016 346 250 reactions The system contains a unique enzyme blend of C. therm. Polymerase and Taq DNA Polymerase. Note: C. therm. Polymerase prov...

Titan One Tube RT-PCR System / Kit

for one-step RT-PCR amplification of up to 6 kb targets Cat. No. 1 888 382 25 reactions Cat. No. 1 855 476100 reactions Cat. No. 1 939 823 50 reactions Components of the Titan One Tube RT-PCR System/Kit allow completion of RT-PCR in a single tub...

Reverse transcriptases suitable for one-step RT-PCRb

city Tolerance<...

Purification of Recombinant Flavonone 3beta-Hydroxylase Using Bio-Scale CHT5-1 Hydroxyapatite Column

Flavanone 3b-hydroxylase (FHT), a typical 2-oxoglutaratedependent dioxygenase, catalyzes the 3b-hydroxylation of 2S-flavanones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavanoids, catechins, proanthocyanidins, and anthocyanidins in plants. To understand the complex reaction mechanism,...

Purification of the Components of Human Macular Carotenoid-Binding Complex, Rev A

The macular region of the human retina is responsible for almost all color vision and most of the high resolution visual acuity. This part of the retina, known as the macula lutea, or yellow spot, accumulates approximately 200 ng of two closely-related compounds, lutein and zeaxanthin....

Sciclone ALH Bulk Dispense Precision Testing

Caliper Life Sciences Inc., Applications Laboratory, Hopkinton, Massachusetts, USA The ability to continuously dispense reagents across multi-well plates and into individual columns (secondary dispensing) is of great importance to high throughput laboratories. Equally important,...

Automated Dissolution of Prednisone with the MultiDose G3

Caliper Life Sciences Corporation, Applications Laboratory, Hopkinton, Massachusetts, USA Prednisone is commonly used in laboratories as a dissolution bath calibrator. Manual dissolution is a very time intensive process; hence automation of this technique is highly d...

96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array

The purpose of this...

Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing

Applied Science and Technology Department, Caliper Life Sciences, Inc., Hopkinton, MA 01748 The High Volume Head is one of four multi-channel liquid handling options available for the Sciclone Advanced Liquid Handler (ALH). The architecture of the device includes an arra...

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

The TempliPhi DNA Sequencing Template Amplification Kit is designed to eliminate...

Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option

Caliper Life Sciences, Inc., Applications Laboratory, Hopkinton, Massachusetts, USA The Z8 liquid handling device is an optional accessory to the Sciclone Advanced Liquid Handler (ALH). This device is designed to permit individually and independently controlled access to single we...

CEOs Gone Wild? Risks and rewards in the executive suite

It happened again. Ano...

Gender hormones may lend to social disorder therapies

Social disorders such as autism constitute one area where those differences come to the fore: around 80 percent of all autism cases, for example, occur in men. Social play behaviors also differ greatly between the sexes - just about everyone agrees that young boys play "rougher" t...

Massachusetts firm to buy UW spinoff Bone Care for $600 million

Bone Care and Genzyme, a 24-year-old company with annual revenues of more than $2 billion and almost 7,000 employees w...

Stem-cell study grows too big for one department; UW builds collaborative programs

Clive Svendsen, a professor of anatomy and neurology, said the university will create a regenerative medicine program and a postdoctoral research program focusing on cell-replacement therapies via stem...

Bone Care marks solid quarter on Hectorol sales jump

Hectorol is a Vitamin D-based drug used to lower abnormally high levels of parathyroid hormone in patients with chronic kidney disease. Gross margins run about 75 percent....

IBM takes one direction, Milwaukee entrepreneurs the other

I spent the morning with Milwaukee entrepreneurs at eForum 2005, where one of the hot topics was the shift from selling services to selling products. The thinking was that it's easier to grow per-employee revenue selling products. Later that day, IBM announced that its year over year earnings were doing...

FDA approves Bone Cares Hectorol for treatment of kidney disease

Middleton, Wis. -- announced Monday the U.S. Food and Drug Administration approved a new indication and strength for Hectorol (doxercalciferol) Capsules. Hectorol, a pro-hormone vitamin D2 analog, is currently approved for the treatment of secondary hyperparathyroidism (SHPT) in dialysis patients in the United Sta...

Where have all the angels gone?

Over the past month, I've seen a half dozen great products that may never see the light of the technology market. The products vary widely from unique hardware devices to much-needed vertical software applications to exciting new mobile services and beyond. Each concept is carefully conceived and well engineered. The market analysis has been done and each product meets a real and unfulfilled need...

Silicon Logic Teams Up to Save Money

Eau Claire, WI-- The business collaboration is meant to speed...

PCR reaction components

The quality of the template influences the outcome of the PCR. For instance, large amounts of RNA in a DNA template can chelate Mg2+ and reduce the yield of the PCR. Also, impure templates may contain polymerase inhibitors that decrease the efficiency of the reaction. Note: To get the purest...

Electroporation of Primary Bone Marrow Cells

, and Lawrence B. Schook, Laboratory of Molecular Immunology, Department of Animal Sciences, University of Illinois, Urbana- Champaign The transfer of genes into eukaryotic cells permits the study of many things, such as gene regulation, gene structure, and gen...

Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses

James R. Wong, Ph.D. and Wei-Yu Lin, Bio-Rad Laboratories A double pulse format for electroporation of mammalian tissue culture cells gave lower transient expression than a single exponential decay pulse. Moreover, separate administration of the two components of the double pulse rev...

Identification of Phase I and Phase II Metabolites of Buspirone on the Q TRAP LC/MS/MS System

Current trends in drug development...

Using THERMAL DESORPTION GAS CHROMATOGRAPHY for the identification of fragrance and other components in cosmetic products

Because of the solid or viscous nature of these formul...

Dispensing 0.5μL to "Dry" 384 Plates with the Sciclone ALH

Caliper Life Sciences Inc, Applications Laboratory, Hopkinton, Massachusetts, USA "Assay-Ready" plates prepared from compound or library stock plates in real time greatly accelerates the High Throughput Screening (HTS) process. The ability to transfer and dilute 0.5μ...

384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler

Caliper Life Sciences Inc, Applications Laboratory, Hopkinton, Massachusetts, USA Initially, work in 96 well microplates was commonly carried out using manual pipetting. As the demand for greater throughput of plate processing has grown, automated liquid handling has become a nece...

Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

Applied Science and Technology Department, Caliper Life Sciences Inc., Hopkinton, MA 01748 Brinkmann An Eppendorf Company, Westbury, NY Eppendorf 5 Prime, Inc. Boul...

HTRF IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers

and Jean-Luc Tardieu Application Note 137 Rev. 12/2005 IP-One assay for Gq...

NeoClone gets $750K for ovarian cancer detection

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Biologist who cloned Dolly the sheep believes therapeutic cloning will gain acceptance

Wilmut, a biologist with the <a href="http://www.re...