OPL

A New PCR-based Mycoplasma Detection Method

Stratagene akit that uses a PCR-based method which specifically amplifies an 874-bpPCR product from a single copy gene. In addition, the kit contains an int...

Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells

Mycoplasma PCR Primer Set for fast and easy detection of mycoplasmacontamination Yale University Genetic Applications LLC Stratagene Mycoplasma contamination of mammalian cell cultures can substantiallyreduce the potenc...

Intracytoplasmic Spermatocyte Injection (ICSI)

The number of infertile couples has increased in recent years and is now estimated at 1520% of all couples worldwide. Since its introduction into reproductive medicine in 1978 , in vitro fertilization has been considered a successful method of treating tubal ste...

Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

The microplate format has become a standard for many biological assays such as high throughput screening for drug discovery and radioimmunoassays. Productivity with these high volume procedures has been limited by the amount of sample handling required and low sample counting throughput. Now, however, microplate assays can be efficiently count...

Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers

Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between synthesis and degradation. Whereas prot...

Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

Evelyn McGown, Ph.D. and Anna Lam, B.S. Molecular Devices Corporation, 8/99 Modern assays for proteolytic activity typically use synthetic chromogenic or flu-orogenic peptide substrates. Enzyme selectivity is obtained by choosing a peptide sequence uniquely recognized by the catalytic site of a particular enzy...

LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)

Anne T. Ferguson, Ph.D. Molecular Devices Corporation This application note describes the use of the SPECTRAmax Viability/Cytoxicity Assay Kit for animal cells. This assay uses two fluorescent dyes, calcein AM (cal...

Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)

By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the NanoOrange Pro software from Mo...

Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

By Yan Zhang., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the OliGreen Pro software from...

PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

Protein concentration is commonly estimated by measuring the absorbance at 280 nm and calculating the concentration using the extinction coefficient of the protein. The procedure is feasible because extinction coefficients are typically obtained using standard cuvettes with a fixed 1 cm pathlength. Extinction-based protein assays can also...

Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)

Most spectrophotometric methods for inorganic phosphorus (Pi) analysis are based on the color formed upon the reduction of a phosphomolybdate complex. The classic (and still widely-used) method is that of Fiske and Subbarow , in which the reduction is carried out with sulfite and aminonaphtholsulfonic acid. Over the years, modi...

UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)

Molecular Devices Corporation. 7/99 In 1994, Molecular Devices introduced SPECTRAmax , the first microplate reader equipped with a monochromator, rather than bandpass filters, for wavelength selection. It was also the first microplate reader capable of measuring absorbance below 340 nm. These new features transformed the micropl...

Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)

Jinfang Liao, M.D., Ph.D. and Evelyn McGown, Ph.D. Molecular Devices Corporation, 8/00 The photoprotein aequorin, a bioluminescent protein from the coelentratejellyfish Aequorea victorea, has proven to be highly sensitive to the trace amount of Ca in cells. The aequorin complex which contains the...

ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Adenosine triphosphate (ATP) is present in all living cells. Because the level isstrictly-controlled, assay of ATP can be used as a indicator of viable cell number.ATP measurements are used to monitor raw materials and manufacturing...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 DNA measurements are important for many aspects of molecular biology. Of thespectrophotometric methods available, UV absorbance measurement at 260 nm isthe simplest and has recently gained widespread popularity in the microplateforma...

High Sensitivity, Wide Dynamic Range, Horseradish Peroxidase (HRP) ELISA Using the LMax Microplate Luminometer (MaxLine Application Note #41)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Immunoassays have traditionally employed colorimetric indicators. As such, multiple dilutions of samples were necessary to ensure that they fell within the dynamic range of the assay. Recently, chemiluminescent immunoassays, with gre...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Reporter gene assays are used to study eukaryotic gene expression. Dual geneticreporters are commonly used in transient transfections of cultured cells tominimize experimental variability caused by differences in cell number, viabili...

Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)

Evelyn McGown, Ph.D. , Michael Su, M.S. and Jin Liao, M.D., Ph.D. Molecular Devices Corporation, 8/00 Luciferase from the North American firefly (Photinus pyralis) is very popular foruse in Reporter Gene Assays. The enzyme catalyzes the ATP-dependent oxidationof luciferin with the concomitant release of light...

Age of empowered individuals: People power behind robust computing

I shouldn't be surprised by the energy in this room today, and neither should you. You are at the very center of an exciting shift in the information technology market. As computing power becomes increasingly distribu...

measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

Cathy Olsen, Ph.D., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089....

Benchmark Plus Microplate Reader Scan Well Feature

Theresa Redila-Flores and Li Tian, PhD, Bio-Rad Laboratories, 2000 AlfredNobel Drive, Hercules, CA 94547 USA The Benchmark Plus microplate reader is a new monochromator- based absorbance reader from Bio-Rad that offers superior convenience, accuracy, and flexibility. The Benchmark Plus is controlled by Microplate Manager...

Cell Proliferation Assay Using the Benchmark Plus Microplate Spectrophotometer

William C Heiser, PhD and Ma Theresa Redila-Flores, Bio-Rad Laboratories, Inc . , Hercules, CA 94547 USA An accurate and rapid assessment of viable cell number and proliferation is often required for in vitro and in vivo experiments. These assessments are necessary and useful for analysi...

Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A

Theresa Redila-Flores, Bio-Rad Laboratories, 2000 Alfred Nobel Drive,Hercules, CA 94547 USA The Bradford and Lowry assays are 2 colorimetric assays that are commonly used to quantitate protein concentration. Bio-Rads protein assay (catalog #500-0001), based on the Bradford dye-binding assay (Bradford 1976), measures t...

Counting Cytostar-T scintillating microplates on the MicroBeta JET

JET is a multi-detector...

Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter

P by Cerenkov counting, a well-known measurement technique that has the...

Technology helps but the best way to reduce Sarbox cost is with people

Investing in your people and culture, which is the essence of entity-wide controls, i...

Two-week notices: People are still concerned

Some don't even know who the...

Power for the people

We pull the plugs, cut the cords, and can the cables as we move deeper into our highly mobile, always-connected, convenience-centered lives. But for all the freedom and mobility, we are nowhere without energy to power the laptops, mobile phones, PDAs, BlackBerries and the like. That fact alone has turned the average business traveler into a Socket Scout, finding pow...

Can tech people be business leaders? Upcoming seminar may have the answer

When Cay Villars worked in marketing for the biotechnology sector, she began to notice a certain pattern: Scientists with a good idea for a new product could find the business world to be a much different place than academia, with a different set of skills required to succeed. "Along the way, I realized that it was that people dynamic inside a company that could make or break a product j...

Berbee puts people first to deliver high-quality technology

Now in its twelfth year, Berbee has channeled these philosophies to create one of the largest and most versatile information technology providers in the Midwe...

Got Game makes big claims; can 90 million people think the same?

Authors compiled personal interviews and surveys to give bu...

Analog people in the Digital Revolution, part II

In my previous column, I started a discussion about what people are good for in the digital revolution. Theres a lot of blah, blah, blah out there about the complexity of current business and technical environments and the need for better judgment, greater flexibility, improved communication and more dynamic creativity. Whats missing from that gray fog of biz-speak are pragmatic courses of action...

Analog people in the digital revolution

Youve heard the drumbeat about IT project failure rates. Youve probably heard mention of all the usual suspects like the lack of sponsor support, poor requirements and scope definition, lack of business relevance or bad communication. What might not be so apparent in all of this hand wringing is the real root cause. Its the people. None of the items listed are technology based. For each one, the...

Expert says network security is about people, not just computers

MILWAUKEE -- Good computer security requires careful attention not just to infrastructure but also to the practices of computer users, according to Debbie Christofferson, president of Whos coming in your door, and do you have it open a crack, or are all your windows open? she asked in a presentation We...