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OLIGO in Biological Technology

Custom siRNA Oligo Synthesis Service

QIAGEN offers a custom siRNA oligonucleotide synthesis service, which includes a range of available modifications. All custom siRNA is synthesized using patented TOM-Amidite chemistry. Two grad...

Cancer siRNA Oligo Set Version 1.0

This comprehensive siRNA discovery tool allows gene-silencing studies of 139 human cancer-related genes for functional genomics research using RNAi. Cancer-related genes were chosen in...

Dalton Pharma Services and ProCitius Announce a Strategic Alliance to Provide Contract Chemistry Services

...e biotechnology and pharmaceutical industries in the areas of chemistry, medicinal chemistry, fine chemical manufacture, custom peptides and antisense oligo production. Dalton provides cGMP manufacturing and sterile filling services to its customers at any stage of the regulatory process (Phase I, II, ...

Sigma-Aldrich Announces Collaboration With Metahelix Life Sciences

...ma-Aldrich's SpyLine(TM) plant mRNA capture kits allow for the isolation of mRNA and performance of RT-PCR in a single 96-well PCR plate incorporating oligo (dT) primers, eliminating transfer steps and increasing throughput. Unique to the market, the Company's Spectrum(TM) Plant Total RNA Kits succeed wher...

Operon Biotechnologies and DNA2.0 Announce Co-Marketing and Technology Development Partnership

...ality' said Jeremy Minshull, President of DNA2.0. 'Operon's fully automated oligo synthesis process meets our exacting quality requirements, and their speed ..., Operon provides a wide range of quality DNA oligonucleotides, Array Ready oligo Sets (AROS(TM)) and pre-printed microarrays (OpArray(TM)). Since its incept...

NimbleGen microarrays speed genetic findings

...d the responsiveness of NimbleGen Systems in producing the specific arrays. The company's ability to "rapidly generate custom-designed, high density oligo arrays targeted to the specific chromosomal regions we were interested in provide us the key data in our study," he said. "Having these tools in ha...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

...A, which facilitates subsequent steps. Priming is accomplished using an oligo with known 5 ends but degenerate 3- ends, and the multicopy DNA sample ...s. These single stranded products are subsequently primed with the same oligo to create double stranded DNA with fixed 5- ends. The resulting library...

Evaluation of FlashPlate in a Helicase Assay

... Procedure 1. DNA oligo #2 was 5'-labeled with 33 P by T4 polynucleotide ...tocol. 2. Equal molar quantities of DNA oligo #1 and the [ 33 P]-labeled #2 oligo were annealed at 90C for 5 minutes and cooled to r...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

...TI software (InforMax, North Bethesda, MD). The melting temperatures (T m ) of the primer and probe sequences were determined by oligo Analyzer 2.5 software (IDT Corporation). Because the sensitivity and reliability of real-time PCR quantitation are dependent on ...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

...ined from normal volunteers. Total RNA was isolated from cryopreserved specimens and 50-100 ng of RNA was reverse transcribed by oligo dT priming. PCR primers for the FasIII region were as previously described 2 with the addition of a 42 bp GC clamp on the forwa...

Primer Walking

...nce Detailed reporting tools Data Quality Agencourt uses custom software for the successful design of oligo sequences for primer walking. Use of our SPRI DNA purification chemistry with ABI 3730xl sequencers provides superior sequencing...

Northern Analysis: The Basics

...mized hybridization buffers and positively charged nylon membranes. Sensitivity can be further improved with oligo dT selection for enrichment of mRNA, since physical constraints of gel electrophoresis and membrane transfer...

Array Analysis: The Basics

... two tissue or cell samples. The RNAs are reverse transcribed using labeled nucleotides and target specific, oligo dT, or random-sequence primers to create two labeled cDNA populations. The two cDNAs are hybridized to two i...

Rapidly Prepare Probes and Markers for Small RNA Analysis

...rent mouse brains or two independent HeLa cell samples with the mir Vana miRNA Isolation Kit. 2.5 pmol of HPLC-purified RNA oligo (28 nt) was labeled and purified with the mir Vana Probe & Marker Kit, and 5 x 10 4 cpm (~10 fmol) were used per solution hybr...

DNA-free A NEW Method to Remove DNA

... 4 centrifugation through a CsCl cushion 5 two rounds of oligo d(T) selection [e.g. FastTrack RNA (Invitrogen), Poly(A)Pure Kit (Ambion)] 6 ...

Get the 5'-End

...ons performed for cDNA library construction are initiated by oligo (dT) primed reverse transcription. This insures the inclusio...ly, for very rare transcripts, Ambion suggests starting with oligo dT-selected RNA to increase the percentage of target in the ...

Is RNA Amplification Necessary for Microarrays?

...eotide random primers; 10 g of Total RNA (unamplified) was labeled with 33 P by reverse transcription using oligo d(T) 15 primers. These targets were hybridized to arrays containing 13,284 human cDNA elements. ...

Five Ways to Produce siRNAs

... 24 hours + DNA oligo ... 5+ days + DNA oligo ~ 6 hours + DNA oligo ...

Bacterial Whole Genome Array Analysis

... A sixth 10 g sample of the same total RNA prep was subjected to a mock MICROB Express procedure without using the kit's rRNA capture oligo mix. Approximately 0.7 g micrograms of the mock reaction was loaded in Lane 7. As seen in the gel image the rRNA bands are virtu...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

...urther enrichment by MICROB Express . The total RNA lane was subjected to a mock MICROB Enrich procedure in which the capture oligo mix was left out of the reaction. RNA was precipitated and an equal fraction of each sample was analyzed by denaturing agarose g...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

...urther enrichment by MICROB Express . The total RNA lane was subjected to a mock MICROB Enrich procedure in which the capture oligo mix was left out of the reaction. RNA was precipitated and an equal fraction of each sample was analyzed by denaturing agarose g...

Bacterial mRNA Isolation, Fast and Easy

...ress Kit. A second 10 g sample of the same total RNA prep was subjected to a mock MICROB Express procedure without the Capture oligo Mix (total RNA). The electropherograms above were generated by running each RNA sample on an Agilent 2100 bioanalyzer using the ...

Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification

...cDNA was produced from yeast total RNA in a 20-l reaction, including 20 g total RNA and a defined copy number of neomycin mRNA (spike-in control), oligo dT primer and avian myeloblastosis virus (AMV) reverse transcriptase. RNA and primers were first denaturated at 70 C for 10 minutes, then incuba...

Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)

...provides a convenient one-tube procedure for the synthesis of ds cDNA, starting even with small amounts of total RNA (120 g). Using the supplied oligo ([dT24] T7 promoter) primer, cDNA is synthesized containing a T7 RNA polymerase promoter for the subsequent transcription reaction with T7 RNA p...

RNAi Gene Silencing System

... custom siRNA oligonucleotide synthesis service , library siRNA directed against common target genes, and a Cancer siRNA oligo Set for screening a large number of cancer-related genes using RNAi techniques. Features and benefits ...

TransMessenger Transfection Reagent

...n results are achieved using high-purity siRNA. The QIAGEN siRNA oligo Synthesis Service offers custom siRNA oligos, a range of library...t of siRNAs targeting genes linked with cancer; the Cancer siRNA oligo Set Version 1.0. Since the amount of siRNA used is a critical fa...

Custom and library siRNA for efficient gene silencing

...service that includes a range of modifications and also library siRNAs directed against common target genes. QIAGEN siRNA oligo Synthesis Service offers: High-purity siRNA for efficient gene silencing Custom and library siRNA ready to use in c...

Custom and library siRNA for efficient gene silencing

...service that includes a range of modifications and also library siRNAs directed against common target genes. QIAGEN siRNA oligo Synthesis Service offers: High-purity siRNA for efficient gene silencing Custom and library siRNA ready to use in ...

TransMessenger Transfection Reagent

...n results are achieved using high-purity siRNA. The QIAGEN siRNA oligo Synthesis Service offers custom siRNA oligos, a range of library...t of siRNAs targeting genes linked with cancer; the Cancer siRNA oligo Set Version 1.0. Since the amount of siRNA used is a critical fa...

QIAGEN Multiplex PCR Handbook

...mentary sequences within a primer sequence and between the primer pair. Commercially available computer software (e.g., oligo 6, Rychlik, 1999) or web-based tools such as Primer3, Steve Rosen & Helen Skaletsky, 2000, (http://wwwgenome.wi.mit.edu/...

QIAGEN Instrument Service insist on the best in service and support

... Cancer siRNA oligo Set Version 1.0 for screening large numbers of gen... The Cancer siRNA oligo Set Version 1.0 is the first disease-specific shor... The Cancer siRNA oligo Set Version 1.0 offers: ...

Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System

...ingle cells. Single cells were lysed in 20 l buffer by pipetting quickly up and down. Following the addition of a biotin-labeled oligo dT-probe to the lysate, the mixture was incubated at 37C for 10 minutes (hybridization of probe and mRNA). The solution was then...

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

... One g of polyA+ RNA (mouse spleen and liver, respectively) were converted into fluorescently labeled cDNA by incubation with an oligo dT primer (44 M final) at 70C for 10 minutes in a final volume of 9 l. The solution was then mixed with 11 l of a solution ...

Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries

...Library Construction Polyadenylated mRNA was isolated from various human tissues or cell lines by two cycles of purification with oligo dT-cellulose. Double-stranded cDNA was synthesized using Stratagenes cDNA synthesis kit, and size-selected cDNA was inserted bet...
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