ODS

Comparison of Different Methods of mRNA Quantitation

<TABLE width="96%" border=0 align="left" cellPadding=8 cellSpacing=1> Nuclease Protection Assays <b...

Comparison of different protein quantitation methods

This application note compares the accuracy and reproducibility ofabsolute protein quantitation of the Agilent 2100 bioanalyzer in combinationwith the Protein 200 Plus assay to the batch-based methodsLowry and Bradford as well as to denaturing gel-elctrophoresis (SDSPAGE)....

RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control

RNase activity varies dramatically across different tissues. A comparison of total RNase activities for 8 different mouse tissues showed that total RNase activity spans a 181,000-fold range from pancreas to brain (Figure 1), which highlights the level of RNase control required for succ...

Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A

Jolanta Kolodziejek, MSc, and Professor Norbert Nowotny, PhD, Clinical Virology Group, Institute of Virology, University of Veterinary Medicine, Vienna , Veterinaerplatz 1, A 1210 Vienna, Austria The purity of the DNA template employed in sequencing PCR (cycle sequencing) is the most...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

Kathleen A. Maguire-Zeiss, Brandon K. Harv e y, Rita E. Giuliano andH o w a rd J. Federo ff, University of Rochester, Rochester, NY, USA Transfection efficiency is dependent upon a number of factors, including method of transfection, cell type, and DNA concentration and purity. Liposome-mediated transfect...

Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

MJ Research, Inc., South San Francisco, CA We present a real-time quantitative PCR (qPCR) protocol for the detection and quantitation of soy genetically modified to be resistant to the herbicide glyphosate. The assay utilizes SYBR Green...

The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A

Andrew Tingey, Reading Scientific Services Ltd., The Lord ZuckermanResearch Centre, Whiteknights, PO Box 234, Reading RG6 6LA, UK One of the most topical issues in the food industry is the use of genetically modified organisms (GMOs) in agriculture and food production. Demands from consumers, particu...

Endotoxins and their Relevance in R&D ApplicationsLPS Detection & Removal Methods

Contaminations of cell cultures show severe problems in the research and production industry. The attention turns here particularly to the prevention of any contamination with bacteria, Mycoplasmas, yeast or fungi. For companies that develop substances for therapeutic applications endotoxins are nowadays a main focus....

Three Micron TSK-GEL ODS-100V Columns for High Throughput LCMS

It is well known in HPLC that columns packed with smaller particles are more efficient at the expense of higher back pressure compared to columns packed with larger particles. The gain in efficiency is usually traded off by reducing column length, which in turn results in shorter analysis...

Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns

While full coverage C18 phases have been responsible for the growth of HPLC as a reliable high efficiency technique, it has become apparent in recent years that such column types are not suited for reliable analysis of highly polar compounds under low organic or aqueous mobile phase conditions...

TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds

Using high purity Type B silica, Tosoh has introduced two new columns with differing surface treatment procedures and carbon content levels to provide unique selectivities for a wide range of sample types. In recent years, many new octad...

Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins

With the introduction of two new reversed-phase column types, TSKgel ODS-100V and TSKgel ODS-100Z, excellent chromatographic performance and selectivity can be achieved for a wide range of sample types. TSKgel ODS-100Z and TSKgel ODS-100V are based on a spherical, five micron, silic...

Technology in the Northwoods

Unless a community or region has a major medical research institution in its backyard, it simply won't become the nation's next biotechnology "hot spot," even if free land and tax inc...

Cub Foods installs biometric payment system

Cub Foods' three stores in the Madison area installed fingerprint scanners last week so shoppers can pay with a swipe of the finger and at lower cost to the supermarket. Scanning a finger calls up a stored profile in the supermarket's computers that includes payment information. Pay By Touch, the San Francisco-based provider of the biometric technology, claims the payments cost retailer...

Midwest shares in stock-market bloodshed as biotechs falter

The stock market, on the other hand, is in the midst of a major blizzard! Results for the first quarter were not pretty almost uniformly across the board. Only the Dow Jones Industrials fared better than o...

Northwoods Software co-owner distills 30 years of business experience

Early in his management career, Bob Weisenberg read a lot of books on management and leadership. One day, when he was about one-third of the way through yet another book, he realized he had read it before. Now retired after a 30-year career in the software industry, Weisenberg says the way to excel at leadership is by getting good at applying a very limited number of skills, not by readi...

Technology puts pressure on old education methods

Judy Brown, an analyst of emerging technology for the University of Wisconsin-Madison and director of the Madison chapter of the World Future Society...

Tech Digest: Modine, Johnson Controls profits; iPods; FBI

Racine-based The company makes heat-management products for trucks and other veh...

Untangling the Web: Northwoods Software in profile

sets itself apart by keeping things simple for clients. Forget the gearhead techno-speak. The Milwaukee area firm is about helping people solve communications and business issues through plain talk and better Web design.<...

North-woods beef jerky maker adopts RFID to track shipments

With the help of Wisconsin-based Jack Links is headquartered in Minong, Wisconsin. ABC Comp...