NSC

Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance

Stratagene We introduce six pairs of control RT-PCR primers that are designed todetect specific human gene transcripts with various expression levels. Theseprimer sets provide insight into whether cDNA synthesis has been effective forall ab...

GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA

A crucial step in gene expression analysis is target labeling. To improve the critical steps in the target-labeling process, QIAGEN has developed the GeneChip Sample Cleanup Module specifically for use with Affymetrix GeneChip expression arrays. The GeneChip Sample Cleanup Module was developed as part of an on...

Suggested applications for reverse transcriptases

High fidelity cDNA for cloning Amplification of viral RNA </s...

Properties of Reverse Transcriptases

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Reverse transcriptases suitable for one-step RT-PCRb

city Tolerance<...

Reverse transcriptases suitable for two-step RT-PCRd

GC-rich...

C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR

Polymerase for two-step RT-PCR amplification of up to 4 kb targets C. therm. Polymerase provided by Roche Applied Science is the recombinant form of the DNA polymerase from the thermophilic eubacterium Carboxydothermus hydrogen...

Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA

One of the RNA silencing products available from Ambion is a kit for the synthesis of siRNA, the <A href="http://www.ambion.com/catalog/CatNum.php?1620">Silencer <A href="http://www.ambion.com/catalog/CatNum.php?16000"> siRNA Construction Kit . While this kit is very cost effective...

Choice of reverse transcription primers

Priming affects the size and specificity of the cDNA produced. There are three types of primers that may be used for reverse transcription: tail at the 3' end of mammalian mRNA. This primer often produces a fu...

Transcriptor Reverse Transcriptase

Increase Power and Sensitivity in cDNA Synthesis with the new Transcriptor Reverse Transcriptase With the new recombinant Transcriptor Reverse Transcriptase, you will Achieve high sensit...

Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase

Transcriptor Reverse Transcriptase is a new recombinantreverse transcriptase (RT) expressed in E. coli. Theenzyme has RNA-directed DNA polymerase activity,DNA-dependent DNA polymerase activity, unwindingactivity, and, importantly, RNase H activity....

Use of Transcriptor Reverse Transcriptase in Microarray Analysis

Researchers studying tissue and cellular differences in gene expressionhave applied microarray analysis to questions ranging from cancer researchto plant development studies. Although the method is well established, manyof the steps in the process continue to be optimised...

Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method

Occurrence of tumor cells in theperipheral blood of individuals sufferingfrom cancer may serve as anearly indication that the primarytumor has dispersed from its tissueof origin. Despite defence mechanismsof the organism, individualcancer cells may attach in di...

High Yield Transcription for Every Application

The MEGAscript Kit is the most frequently cited large-scale transcription kit, and with good reason. It enables the consistent transcription of high yields of full-length RNA (Figure 1). Ambion offers a complete line...

Recombinant HIV Reverse Transcriptase

Ideal for reverse transcribing transcripts with high G/C content...

Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs

siRNA Construction Kit uses an in vitro transcription reaction and column purification to prepare transfection-ready siRNAs. With this kit, multiple siRNAs can be prepared in ~24 hours. Below is one example of how siRNAs prepared by the Silenc...

Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs

Ambion has developed a system for siRNA synthesis and purification based on in vitro transcription, the siRNA Construction Kit (patent pending). This kit produces transfection-ready siRNA at a fraction of the cost of chemical synthesis. Here, resear...

A simple and rapid LC-MS/MS method for the simultaneous determination of nine antiretroviral drugs commonly used in Europe (protease Inhibitors and non-nucleoside reverse transcriptase inhibitors)

In clinical research, the monitoring of protease inhibitor (PI) and non-nucleoside reverse transcriptase inhibitor (NNRTI) antiretroviral drugs is of significant interest due to the importance of assessing inter-individual and intra-individual metabolism variability. To be of practical use, it is essential that the analytical method used to mon...

States need to be more "connectivity conscious"

Having a good infrastructure has always been important to the economic growth of a municipality as well as a state. The infrastructure has traditionally been viewed as transportation - rail, waterways, and air as well as highways, water, and power (electricity). Today, the definition of infrastructure must also include network infrastructure and the ability to have...