NIST

Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000

5, a pH sensitive dye, has shown utility in β2-adrenergic receptor agonist screening (1). CypHer5 has been used to obtain dose-response and rank-order potency data for both agonist and antagonist treatment of β2-adrenergic receptor expressing cells (2). For live-cell receptor internalization studies,...

Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000

High-throughput screening for potential targets is a major requirement in drug development and a key application is in the area of receptor activation, particularly G-protein coupled receptors (GPCRs) (1). <P >It is estimated that there are still 100–200 orphan GPCRs of potential therapeutic value requiring identification of...

The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists

Beta-agonists are group A substances of the European guideline 93/257/EWG (revision 98/536/EG) having an anabolic effect and not licensed. Thus, they have to be determined in veterinarian samples like muscle, liver, retina, and urine. The coupling of High Pressure Liquid Chromatography (HPLC) and Electrospray Ionization Tandem Mass Spectrometry (ESI-MS-MS) seems to be the analytical m...

Obstructionists block network routing diversity

Making network infrastructure more reliable in this post 9/11 era should be everyone's goal, whether they are part of the government, a network carrier, or a business with mission-critical network needs. This endeavor should transcend any petty competitive issue. Having the best network infrastructure globally is a matter of national defense as well as strategic nat...

Culver's in biofuel deal; net extortionists; no net tax

Culver's, a fast-food chain native to Wisconsin, is working with Madison Environmental Group on a kit to make cars run on old frying oil. Meanwhile, online extortionists are holding personal files for ransom, and a proposed Wisconsin sales tax for online entertainment has been rejected. Original air date: Saturday, May 28, 2005 Listen t...

Northwest Wis. Manufacturing Outreach Center honored by DOC, NIST

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Raising a Biotech Workforce - Students, Program Administrators Look to Future

MADISON, Wis.Marshalling a biotech-savvy workforce for the states burgeoning life sciences industry is going to take money, time and secondary-school awarenessall of which Wisconsin could use more of. Speakers and attendees alike at the 20th Annual Life Sciences and Venture Conference at the Monona Terrace Convention Center this week looked forward to the day when the state will be crank...