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Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling

Danny Q. Hoang Michael Browning Xinping Yang PeterPingerelli Stratagene Cloning Systems, Inc. Stratagene introduces affinity purified antibodies for NMDA receptorsubunits that are useful for studying expression of the NR2A, NR2B and NR2Csubunit proteins...

Enzyme Immunoassay for Studying Intracellular Levels of cAMP

Sensitive, nonradioactive cyclic-AMP detection kit and adenylate cyclase(AC) toxin Stratagene Cloning Systems, Inc. American Qualex Stratagenes EIA cAMP Kit is a highly sensitive and specificantibody-based assay kit for no...

Funds will support public safety communications interoperabilty

The funds will be used to implement a regional public safety mobile radio plan for the 16-county group, and purchase equipment that will ena...

UW scientist wins Pew grant to study little-known immune cells

Jenny Gumperz, an assistant professor of medical microbiology and immunology, will receive $60,000 per year for the next four years from the Pew Charitable Trusts, an independent nonprofit organization that annua...

DEMO 2003 Closes with Focus on Collaboration and Communication

Scottsdale, AZ - DEMO 2003 continued to buzz with innovation and excitement on its closing day, focusing on a myriad of new technologies that included timely next-generation systems designed to protect and defend the national IT infrastructure as well as collaboration tools and evolving email services. In addition to an array of new product demonstrations, technology leaders discussed the possibi...

Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A

Menu B. Leddy, Biotechnology Research Department,Orange County Water District, 10500 Ellis Ave., Fountain Valley, CA 92708 Identification and characterization of bacterial species from membrane biofilms has been a microbiological challenge. A vast majority (>90%) of bacterial cells from membrane...

Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A

and Aiguo Zhang, 1 Department of Molecular and Clinical Medicine, Division of Paediatrics, Linkping University, Sweden, and Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA...

Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B

Diana Davis, PhD, Aiguo Zhang, PhD, Chloe Etienne, Ivan Huang,and Michele Malit, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive,Hercules, CA 94547 USA Bio-Plex cytokine assays are bead-based multiplex sandwich immunoassays. The concentrations of analytes in a Bio-Plex cytokine immunoassay are...

Real-Time Immuno-PCR on the iCycler iQ System, Rev A

Kristina Lind and Mikael Kubista, TATAA Biocenter, Medicinaregatan 7A/B,405 30 Gteborg, Sweden A technique for antigen detection, called immuno-PCR, was developed by Sano et al. (1992). It combines the molecular recognition of antibodies with the high DNA amplification capability of PCR. The procedu...

Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C

Eric Larson, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA PVDF membrane was originally introduced to protein use as an ideal medium for the harsh chemical environment of N-terminal, or Edman degradation, sequencing. Even though PVDF is very hydrophobic and...

A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)

A previously published application note in this series describes an LC/MS/MS method for the simultaneous determination of four immunosuppressant drugs: Cyclosporin A (CsA), Tacrolimus (TAC, aka FK-506), Sirolimus (SIR, aka Rapamycin) and Everolimus (EVE, aka RAD-001) (1, 2). The method is based on a simple and robust hardware configuration and...

Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System

Immunoassays are valuable and widely used tools in the biological sciences. In drug discovery research, immunoassays are used to measure secreted cytokines in the tissue culture supernatants of treated cells. However, scientists often avoid immunoassays in high throughput screening (HTS) because they require cumbersome manipulations. Applied B...

Affinity Depletion Cartridges for Removal of Human Serum Albumin and Immunoglobulins from Human Serum

One of the major difficulties in analyzing the proteome of human serum is the dynamic range of the concentrations of the proteins present in the sample. Human serum albumin (HSA) constitutes 57-71% of total serum protein and gammaimmunoglobulin (IgG) ranges from 8-26%. Removal of these two proteins alone clears about 75% of t...

Coated Plate Immunoassay on the MicroBeta - eg. TSH - IRMA

Th...

Immunoassay of Theophylline by fluorescence polarisation

Fluorescence immunoassay is applied to the detection of theophylline, a drug used for myocardial stimulation. Interference from other sample components is limited by use of polarization measurements, which discriminate between bound and unbound fluorophores. The detection system includes a T-format sampling module mounting two emissio...

Small Tree Communications awarded $2.85M defense contract

This funding, part of the FY 2007 defense appropriations bill, was awarded through a competitive award process. It will enable the company t...

New Urbanism: Community planning Feng Shui

The need to have a rebirth of the American municipality. The need to create a solid platform for economic...

Branding around the community brand concept

Now this require...

Sale of Norlight Telecommunications is finalized

The sale, subject to federal and state regulatory approvals, is expected to take four to six months to complete.<b...

Virtual communities make online connections

(according to <a href="http...

Journal to sell Norlight Telecommunications

Q-Comm and Journal Communications have signed a definiti...

No time for evolution in telecommunications

- There is a revolution taking place in the telecom industry. Not an evolution - we don't have that much time. Today, a dynamic shift is being driven by technological advances that, in turn, drive a convergence of many formerly discreet industries into one; that, in turn, create hyper-competition among long established and brand new companies;...

Accelerate Madison to merge with Telecommunications Professionals

The organization said the partnership "reflects the increasingly blurred line between the telecommun...

Creativity, innovation extend to municipalities

Century." At that time, many who attended thought it was a novel idea but did not put that much faith in it. My argument was that the horse-and-buggy taxes that focused on gasoline, cigarettes, and liquor were...

Online communities may be next business market

Ramakrishnan, a vice president and research fellow at But if his preference trumps hi...

Nano-diamond film studied at UW could advance telecommunications

But these are no ordinary crystals. Teams of scientists across the country, pursuing a technology 14 years in the makin...

Municipal broadband in Illinois: Where are the standards?

Quinn had some encouraging remarks about keeping the playing field open f...

Electronic signs for municipalities: Las Vegas or lost revenues?

This is a...

Journal Communications to spin off Norlight

The spin-off will be completed through a pro-rata distribution of Norlight's shares to shareholders of Journal...

Control the community or just let go?

That advice may never have been so apt as in the case of Wetpaint, which t...

CoreComm slapped by AG for violating telecommunications laws

CoreComm, a Delaware corporation, provides dial-up internet access and related services to Wisconsin customers, many of whom originally subscribed to ExecPC, a Wisconsin-based inter...

Immune boosting beverage relying on Milwaukee firm's packaging technology

New York-based is launching Defense Vitami...

Electronic Tele-Communications reports continued challenges

WAUKESHA, Wis. - The company, which supplies voice application processing platforms to domestic and foreign telephone utilities and of messaging systems to the commerci...

UW Colleges selects Allot Communications to manage multi-campus network

"Allot's NetEnforcer is indispensable to our remote IT staff because it helps us implement global network policies, optimize network efficiency and carefully control user-...

Paper tech center will connect research with economic and community need

Launched last week with the help of Wisconsin's congressional delegation, tw...

UW scientists study strange material with communications potential

Scientists at the University of Wisconsin-Madison are taking another look at some long-held ideas about physics. For one, the Doppler effect. That's what scientists call the way sounds change as you speed toward and away from them. On a highway, for example, a jackhammer or a siren will sound higher pitched as you approach and lower after you drive past. But there's an invers...

Midwest cities in fight over municipal broadband access

Did you notice the historical significance of this past Sunday? Yes, it was Mother's Day, and yes, it was the 60th anniversary of V-E Day ("Victory in Europe"), but it was also the 106th anniversary of the birth of Friedrich Hayek. He is one of the world's greatest economists. These days, while Hayek is mostly remembered for his destruction of socialist hokum in his seminal work "The Roa...

More electronic gadgets, more miscommunication

Attention gadget guys and girls this is your time. The shops are loaded with electronic communications gizmos of all kinds this holiday season. But when it comes to the value of these devices opinion is increasingly split. Many people either love them or loathe them. I guess I fall into the latter category. Of course I use a computer; Im not a total Luddite (although when I read the Un...

VC Community Shares Perspectives on Midwest Life Science Funding

The Amex Biotech Index includes only about 20 biotech stocks...

UL to provide Datasyst with telecommunications certification testing

DELAFIELD, Wis.- Datasyst Engineering and Testing Services and Underwriters Laboratories have announced they will work together on certifying network equipment building systems (NEBS) tests for telecommunications suppliers. The tests cover components of the systems such as seismic and transportation vibration, heat, humidity, and altitude. Und...