Microdissection in Biological Technology

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

Vasker Bhattacherjee, Partha Mukhopadhyay, Saurabh Singh, Robert M. Greene and M. Michele Pisano, University of Louisville Birth Defects Center, Department of Molecular, Cellular and Craniofacial Biology, Louisville, KY 40202 Abstract This application note describes a Las...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

Sven Fraterman and Neal Rubinstein, Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia Abstract To locate gene products in distinct muscle fiber populations of different muscle allotypes, a novel protocol was establi...

Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

Se-Jin Yoon and Kyung-Ah Lee Infertility Medical Center, CHA General Hospital, Seoul 135-081, Korea Abstract Laser Capture Microdissection (LCM) is an innovative technique which permits the rapid and reliable procurement of pure cell populations from heterogeneous tissue sec...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

(Anthony Lombardino, Rockefeller University) Abstract Brain tissue is notoriously heterogeneous, and accurate analysis of neural gene expression requires procurement of specific cell types. In this application note a technique is described for using Laser Capture Microd...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

Dr. Eugene Trogan, Ph.D., Mount Sinai School of Medicine of New York University Abstract Macrophage foam cells are integral in the development of atherosclerotic lesions, however gene expression studies are complicated by the cellular heterogeneity of atherosclerotic plaque. This ap...

Working With Laser Capture Microdissection Samples

Laser Capture Microdissection (LCM) is a method for obtaining pure populations of cells from heterogeneous samples. LCM permits the selection and capture of cells, cell aggregates, and discrete morphological structures from thin tis...

Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation

Stefanie Schrer, Werner Wittke,* and Christian Kupfer QIAGEN GmbH, Hilden, Germany * Leica Microsystems, Wetzlar, Germany Laser microdissection (LMD) provides a powerful t...

Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection

StrataPrep total RNA microprep kit for very small samples Karen Dolter Sylvia Norman Jeff Braman Stratagene Isolating total RNA from laser capture microd...

Danong Chen appointed CEO of Theranostics Health

... a privately held company founded in 2006. Its core technology is the breakthrough Reverse Phase Protein Microarray (RPMA) combined with Laser Capture microdissection (LCM). The technology measures the activity of a large number of biomarkers, enabling pharmaceutical companies to accurately profile their drug candid...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

...FPE samples . Br. J. Cancer 94 : 333-7. 8. Ldecke H., et al., (1989) Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification . Nature, 338 : 348-50 9. Pirker C., et al., (2004) Whole genome amplificat...

Microgenomic Expression Profiling

...samples as limited as a single cell. Laser capture microdissection (LCM) is an advanced separation technology that en...s from heterogeneous tissue samples (Veritas Laser microdissection System, Arcturus Bioscience). LCM utilizes an infr...mounts of RNA (≤2000 cells). Following laser microdissection of the desired cell population, RNA is isolated an...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

...We wanted to determine the frequency of these mutations in ductal lesions and associated tissues of tumor free pancreases. We used microdissection followed by a sensitive single step PCR regime to amplify the exon 1 of the K-ras gene. Point mutations were detected by constant ...

RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay

...hen working with limited samples, such as small biopsies, mRNA-poor cells and tissues, primary cultured cells, and laser capture microdissection (LCM) samples, and, in this example, cells selected and collected by flow cytometry. RNA amplification is needed to perform arra...

RNA Amplification Just Got Better: MessageAmp II

... samples, such as small biopsies, mRNA-deficient cells and tissues, primary cell cultures, and laser capture microdissection (LCM) samples. This hurdle is usually overcome by using T7 RNA polymerase driven transcription of cDNA obtai...

RNA from LCM Samples

...ression profiles of specific cell types. Laser capture microdissection (LCM), developed in a collaborative effort between the...ained with cresyl violet. (B) Same section after Laser Capture microdissection using PixCell IIe LCM workstation (Arcturus). Note absence of ta...

RNA Amplification for Array Analysis

...ling and hybridization. Thus, microarray-based gene expression analysis of very small samples [laser capture microdissection (LCM), tissue biopsies, or other clinical samples] is difficult due to the very low amounts of total RNA rec...

Protect RNA and Improve Target Cell Identification

... LCM for RNA Analyses Laser capture microdissection (for overview see Laser Capture microdissection (LCM): How its Done , at right) is a method that allow...

Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis

... RNAqueous-Micro Kit Ideal for Processing Laser Capture microdissection Samples Further development of Ambion's ...pus was stained with Acridine Orange (LCM Staining Kit, Ambion). microdissection with the PixCell IIe LCM System (Arcturus) was performed with th...

Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.

... Gene expression profiling of material isolated by microdissection has become a very popular method for analyzing c...search and clinical applications. Laser-assisted microdissection allows isolation of RNA from specific cell popul... study Tissue preparation and microdissection Snap-frozen mouse liver tissue...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

...triction sites is avoided. On another exciting front, the kit can be used to make cDNA libraries from tissue samples isolated by laser-capture microdissection (LCM). 4 This technique, developed at the National Institutes of Health, captures pure populations of targeted cells from microscopic samples of ...