Master

TripleMaster PCR System

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TripleMaster PCR System

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Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq

University of Georgia, Department of Marine Sciences This application describes the use of the MasterTaq polymerase kit for improving PCR efficiency when studying functio...

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

BIOGEM, Division of Biology, University of California San Diego, La Jolla, CA 92093-0349 e-mail: ghardiman@ucsd.edu www.biogem.ucsd.edu Microarray technology, primarily advanced by Pat...

cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR

RT-PCR has become an essential method that allows researchers to determine the presence, structure and level of expression of an mRNA transcript in the organism, tissue or cell of interest. The basic protocol involves the synthesis of a cDNA copy of all the mRNA present in the s...

Efficient and Reliable PCR Setup Using Eppendorf MasterMix

Christian Rohrer, Eppendorf AG, Germany Lars-Erik Peters, Eppendorf 5Prime, USA The reliability of a PCR* experiment is dependent on its sensitivity, specificity and reproducibility. An essential prerequisite for a succ...

HotMasterAn innovative Hot Start/Cold Stop technology for better PCR* results

George Halley, Eppendorf 5 Prime, Inc., Boulder, CO, USA Vincent Prezioso, Brinkmann Instruments, BioSytems Application Lab, Westbury, NY Hot Start DNA polymerases have become very popular because of their conven...

Enhancing PCR* Efficiency with MasterTaq

Experimental Immunopharmacology, Research Center Borstel, Parkallee 22, D-23845 Borstel, Germany. Tel.: +49 4537 188458 Fax: +49 4537 188404 email: jvdbosch@fz-borstel.de This application describes the use of a n...

Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System

Martin Simon, Petra Albrecht, Helmut J. Schmidt, and Andrs Jarrin Martin Simon, Petra Albrecht, and Helmut J. Schmidt, University of Kaiserslautern, Germany, Department of Ecology Andrs Jarrin, Eppendorf AG With the help of the Eppendorf cM...

The use of TaqMaster PCR Enhancer

Guy Adami, Department of Oral Medicine, University of Illinois in Chicago, USA Vincent Prezioso, Eppendorf Scientific, Inc., Westbury, NY, USA Transgenic mice carry a fragment of foreign DNA that has been incorporated into their g...

The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA

Max-Planck-Institute for Marine Microbiology, Celsiusstr.1, 28359 Germany Phone: ++49-421-2028-936, Fax: ++49-421-2028-580, email: kravensc@mpi-bremen.de Direct extraction of total DNA from soils or sediments always results in...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In man...

Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler

Peter Scheinert, PhD, Barbara Behrens, PhD, and Dietmar Kahle, PhD Peter Scheinert, PhD, Bernhard-Nocht-Institute for Tropical Medicine, Virology Dept., Bernhard-Nocht-Str. 74, D-20359 Hamburg former address: University of Kiel, Institute for General Microbiology, Am Botanischen Garten, D-24118 K...

Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient

Using Eppendorf Mastercycler gradient NPC Laboratory, Institute of Postgraduate Studies and Research, University of Malaya, Kuala Lumpur, Malaysia...

The use of the Mastercycler gradient for single-cell PCR and preimplantation diagnosis

Karen Sermon, M.D., PhD Center for Medical Genetics, University Hospital and Medical School of the Dutch-speaking Brussels Free University, Laarbeeklaan 101, 1090 Brussels, Belgium Preimplantation diagnosi...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In many research projects, cD...

Eppendorf Mastercycler ABI Big Dye Sequencing Protocol

From MAIN MENU go to FILES/ EDIT/ STANDARD rewrite accordingly then EXIT/ YES (use SEL to choose)/ ENTER/ change name to BIGDYE/ ENTER...

The TripleMaster PCR System for three different amplifications

Christian Rohrer, Eppendorf AG Standard PCR components (Taq polymerase and reaction buffers) are used for the general amplification of relatively short DNA fragments (100 bp to roughly 2,000 bp). However, demanding...

Mastercycler Gradient Quick Programming Guide

USE ARROW KEYS TO SCROLL THROUGH MENUS AND PROGRAMS. PRESS ENTER TO SELECT HIGHLIGHTED CHOICE) Menu items are chosen by first positioning cursor to the choice desired, then press . This confirms choice or input data or changes display to next menu in hierarchy....

TripleMaster PCR System: Direct Comparison with Competitors

Eppendorf 5 Prime, Inc., Boulder, CO, USA This experiment was performed to compare the Eppendorf TripleMaster PCR System with the long-range PCR products of leading competitors. Eppendorf was compared to competitors T...

TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates

to Amplify Long gDNA Templates Eppendorf 5 Prime, Boulder, CO, USA Eppendorf provides a complete system for long-range PCR1 analysis of gDNA. The Perfect gDNA Bloo...

TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

Eppendorf 5 Prime, Boulder, CO, USA PCR* technology has been widely used in molecular biology research, providing scientists with an efficient tool for studies such as genome analysis, sequenci...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

Eppendorf 5 Prime, Boulder, CO, USA The polymerase chain reaction (PCR*) is a powerful and sensitive technique used by scientists to amplify large quantities of DNA. Although the reaction mix and thermal cycling conditi...

High Fidelity PCR Master

for amplification of up to 5 kb targets from genomic DNA Cat. No. 2 140 314 200 reactions Premixed, 2x concentrated High Fidelity PCR Master contains all necessary reagents (buffers, nucleotides and enzymes) needed for PCR. The mix contains a unique enzyme blend of Ta...

PCR Master

for routine amplification of up to 3 kb genomic DNA targets Cat. No. 1 636 103 (100 reactions) The PCR Master Kit contains 10 vials of 2x concentrated PCR reaction mix (Taq DNA Polymerase, reaction buffer with MgCl , stabilizing detergent, dNTPs) plus water PCR...

Pwo Master*: Simplify Setup of Your Proofreading PCRs

With the introduction of the Pwo Master, Roche AppliedScience now offers a new, ready-to-use proofreadingmaster mix for the robust amplification of fragments up to3 kb, providing the highest possible fidelity during PCRsynthesis....

New UW master's program ramps up to fill demand for biotech skills

students will walk across the stage for their diplomas at the University of Wisconsin-Madison this weekend. For the past two years they've worked closely within a group of 20 classmates, up from last year's pilot class of 10, to get deeper sense of the three inseparable d...

NameProtect and MasterCard unite to combat online fraud, phishing

MADISON, Wis. Under the arrangement, NameProtect...