Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

Abstract Applied Biosystems has developed new techniques that produce highpurity total RNA from biological sources such as cultured cells. These techniques eliminate the need for post-isolation DNase treatment or other manual handling, and produce a highly pure product, free ...

Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A

Heping Han*, Biren Zhao*, Robert Hsueh # , and Susanne Mumby*, *The Antibody Laboratory and the # Cell Preparation and Analysis Laboratory, The Alliance for Cellular Signaling, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390 ...

Micromet to Present Six Posters on Progress of BiTE Antibodies at the Annual Meeting of American Association for Cancer Research

... Lutterbuese, R. et al. (2009). Highly efficient lysis of KRAS- and BRAF-mutated human colon cancer cells...ake a major contribution to redirected target cell lysis by T cell-engaging BiTE antibody MT110. AACR Annua... Aigner, M. et al. (2009). Effective in vitro lysis of human melanoma cell lines by T lymphocytes redi...

InVitria Among Leading Innovators in Cell Culture Media According to Top Biotechnology Publication

...nes. Also mentioned in the article is InVitria's Lysobac™, a cell lysis reagent used in microbial fermentation to improve recombinant protein yield...ble, well-defined and regulatory compliant cell culture media and bacterial lysis components. They can also contribute to operational efficiency and reduced ...

Proteolix Announces Positive Data From Two Ongoing Phase 2 Clinical Trials of Carfilzomib in Multiple Myeloma at the 50th Annual Meeting of the American Society of Hematology

...ated among relapsed patients and toxicities were manageable. The most common adverse events were fatigue, anemia and neutropenia. Two cases of tumor lysis syndrome were observed among bortezomib-naive patients, and with additional monitoring and management guidelines no further events have been reported....

InVitria Touts Product Advantages at Cambridge's Cell Culture Conference

...actor that also delivers iron similar to transferrin in cell culture media. (see http://www.Lacromin.com ) -- Lysobac - Lysobac is a bacterial cell lysis agent that is more efficient and more consistent compared to chicken lysozyme and provides higher recombinant protein yield compared with mechanical c...

Microchip Biotechnologies, Inc. Awarded Contract Under US Army Commercialization Pilot Program

...will integrate multiple sample processing steps in a small, universal sample preparation module that integrates immunomagnetic separation capture with lysis and nucleic acid purification. "Easy-to-use sample preparation, under field conditions, has always been a major obstacle to deploying back end patho...

Jennerex Completes $8.3 Million Series B Financing and Appoints Dr. Cal Stiller as Director

... high levels in cancer cells. To enhance product efficacy, JX-594 is also engineered to express the GM-CSF protein. GM-CSF complements the cancer cell lysis work of the product candidate, leading to a cascade of events resulting in tumor necrosis, tumor vasculature shutdown and an anti- tumoral immune atta...

InVitria Sponsors Cambridge Healthtech's Optimizing Cell Culture Development Conference

...h promotant as a cell culture ingredient in mammalian cell culture media. (see http://www.Lacromin.com ) -- Lysobac -- Lysobac is a bacterial cell lysis agent that is more efficient and more consistent compared to chicken lysozyme and provides higher protein yield compared with mechanical cell lysis. L...

InVitria Further Strengthens Sales Team with Promotion of Brandy Sargent to Account Manager - Western United States

...dition, Lacromin is a strong growth promotant as a cell culture ingredient in mammalian cell culture media. -- Lysobac - Lysobac is a bacterial cell lysis agent that is more efficient and more consistent compared to chicken lysozyme and provides higher protein yield compared with mechanical cell lysis. L...

InVitria Expands Sales Team

...safety versus bovine and human transferrin. And Lysobac is a bacterial cell lysis agent that is more efficient and consistent compared to chicken lysozyme. ...pplement for mammalian cell culture media. -- Lysobac -- A bacterial cell lysis agent that is more efficient and more consistent compared to chicken lysozy...

Sigma-Aldrich Announces Collaboration With Metahelix Life Sciences

...creasing throughput. Unique to the market, the Company's Spectrum(TM) Plant Total RNA Kits succeed where most protocols fail, employing patent-pending lysis and binding chemistry to purify total RNA from plant tissues that contain high levels of secondary metabolites. To learn more about Sigma-Aldrich's ...

Millennium Announces VELCADE(R) (Bortezomib) for Injection First-Quarter 2008 U.S. Net Sales

...hy, hypotension observed throughout therapy, cardiac and pulmonary disorders, gastrointestinal adverse events, thrombocytopenia, neutropenia and tumor lysis syndrome. Women of childbearing potential should avoid becoming pregnant while being treated with VELCADE. Cases of severe sensory and motor periphera...

Major Medical Journal Reports Higher Success, Fewer Complications and Lower Cost Treating DVT With Trellis(R) Isolated Thrombolysis Compared With Catheter-Directed Thrombolysis

...conventional catheter-directed thrombolysis (CDT) in the treatment of DVT in its March 2008 issue. Results of the study showed that Grade II and III lysis was achieved in 93% of patients treated with the Trellis catheter and 79% of patients treated with CDT even though thrombolytic doses and infusion dur...

Millennium Over-Delivers on 2007 Goals and Financial Guidance

...hy, hypotension observed throughout therapy, cardiac and pulmonary disorders, gastrointestinal adverse events, thrombocytopenia, neutropenia and tumor lysis syndrome. Women of childbearing potential should avoid becoming pregnant while being treated with VELCADE. Cases of severe sensory and motor periphera...

Millennium Announces 2008 Goals for Growth, Building on Record 2007 Results

...hy, hypotension observed throughout therapy, cardiac and pulmonary disorders, gastrointestinal adverse events, thrombocytopenia, neutropenia and tumor lysis syndrome. Women of childbearing potential should avoid becoming pregnant while being treated with VELCADE. Cases of severe sensory and motor periphera...

Millennium to Receive $40 Million Milestone Payment for Achievement of Sales Threshold for Velcade(R) (Bortezomib) for Injection

...hy, hypotension observed throughout therapy, cardiac and pulmonary disorders, gastrointestinal adverse events, thrombocytopenia, neutropenia and tumor lysis syndrome. Women of childbearing potential should avoid becoming pregnant while being treated with VELCADE. Cases of severe sensory and motor periphera...

Millennium Reports Outstanding Third-Quarter Results Driven by VELCADE(R) (Bortezomib) for Injection Sales

...hy, hypotension observed throughout therapy, cardiac and pulmonary disorders, gastrointestinal adverse events, thrombocytopenia, neutropenia and tumor lysis syndrome. Women of childbearing potential should avoid becoming pregnant while being treated with VELCADE. Cases of severe sensory and motor periphera...

Genta Completes Appeal of FDA Decision for Genasense(R) in CLL

...alone group had adverse events that resulted in death, including two patients in the Genasense group who died from complications associated with tumor lysis and infusion-related reactions. About Chronic Lymphocytic Leukemia CLL is the most common form of leukemia in adults. According to the American Canc...

Micromet is Presenting Four Posters on BiTE(R) Antibodies at the International AACR-NCI-EORTC Conference

...ctive against melanoma cells by recruiting T cells for efficient tumor cell lysis in vitro (abstract A61). This poster has been selected for inclusion in AAC...EpCAM that is expressed on healthy epithelial cells. The mode of tumor cell lysis of MT110 was found to be largely dependent on the pore-forming protein perf...

BoVir(R) Named Best New Veterinary Diagnostics Product in International Animal Pharm Awards

...vides unprecedented early detection of the virus, and it produces the most advanced genotype and strain diversity detection. "Our unique, proprietary lysis buffer eliminates the need for the costly, labor-intensive, and time-consuming RNA extraction step, which further eliminates the complicated error-pro...

Arius Features Preclinical Data on Antibody Candidates at Two Conferences

...ptember 8-11, Arius will report results demonstrating that its monoclonal antibody targeting CD59, a cell surface molecule involved in regulating cell lysis mediated by the complement system, is a potent therapeutic in various in vivo cancer models. Arius has demonstrated that its antibody is highly potent...

EMD Biosciences partners with European biotech company

...sortium of leading experts from three major pharmaceutical companies. The system combines bacterial and insect cell culture with automated harvesting, lysis and purification units. It is designed to allow scientists to rapidly identify the best construct and host strain, and recreate optimal culture condit...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

... reagents to effect a very gentle extraction. The lysis uses a combination of proteinase K and lytic det...act-N-Amp ™ reagent line. Efficient, gentle lysis and a robust amplification should give the great.... The DOP amplification mechanism and the gentle lysis included in the GenomePlex Tissue WGA kit make i...

Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System

... purposes. Harvesting: You can harvest cells, cell components, or intracellular protein by trypsinization, lysis buffer, freeze-and-thaw, sonication or any chemical or physical method. Specific protocols are available in New Brunswick Scientifics FibraStage...

QuickLyse Miniprep Kit

... The QIAGEN QuickLyse Miniprep Kit uses one-step lysis technology for ultra-fast preparation o...ckLyse Miniprep Kit include: One-step lysis reduced number of handling steps saves ... One-step lysis for fast results Unlike most protocol...

TissueRuptor System

... Flexibility compatible with different sample types, lysis buffers, and disruption vessels ...when using the QIAamp DNA Mini Kit for DNA purification as the lysis step with proteinase K can be reduced to just 1 hour. Purified D...

HTRF IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers

...erally 30 minutes at 37C. Quantification of the accumulated IP1 is obtained after dispensing the two diluted conjugates into the lysis solution. Measurements can then start to be taken after just one hour of incubation, and be repeated as many times as necessary ...

High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation

...100 μL tips 2 . Add 95 μL Resuspension Solution and shake @ 1200-1500 rpm for 4 minutes 3. Add 100 μL lysis Solution and shake @ 800-1000 rpm for 5 minutes 4. Add 100 μL Neutralization Solution and shake @ 800-1000 rpm for 10 minutes ...

Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)

...olecular Devices Lmax microplate luminometer. Cell lysis and enzyme detection occur upon addition of the si...tely 6 fg/well. For measuring luciferase in cells, lysis and enzyme activity occur almost instantaneously i... of the fact that both the flash reaction and cell lysis appear to be maximal within 2 seconds (see Figures...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

...3B); Stop & Glo Substrate Solvent (E463A); Passive lysis buffer, 5x reagent (E194A). 2. Firefly Lu...SE STANDARDS 1. Passive lysis buffer was prepared by mixing 2 mL of passive lysis 5x reagent (E194A) with 8 mL deionized water. ...

Measuring siRNA-mediated knockdown of IL-8 mRNA

... 175 l. Cells were transfected at 37C for four hours, followed by recovery for either four or eight hours in 100 l fresh complete DMEM. The QuantiGene lysis Working Reagent was added to each treatment group for an additional 30 minutes. The QuantiGene assay was performed according to manufacturers instruct...

QBT Fatty Acid Uptake Assay Kit datasheet

...metabolic diseases requires robust assays for the quantitation of fatty acid uptake. Conventional protocols utilizing radioactivity often require cell lysis and processing at very low temperature, making them expensive, slow and not suitable for high throughput screening. Fluorescence-based protocols gener...

A highly sensitive ATP-luminescence assay using VICTOR2 with the dispenser option

...56;L of Luciferase reagent was dispensed into white microplates (Combiplate, Labsystems, Finland) with 25 μL of ATP sample and 25 μL of cell lysis reagent. The wells were measured for 10 seconds after a 1 s delay. RESULTS The dilution series proved to be linear over 5 decad...

Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3', 5' monophosphate and its validation for pharmacological screening at G protein-coupled receptors

...micro;l) containing labeled cAMP (enzyme donor [ED] fragment), inactive EFC enzyme (enzyme acceptor [EA] fragment), cAMP monoclonal antibody, and cell lysis reagent. Assay plates were incubated overnight before measurement on the LEADseeker Multimodality Imaging System.

Results...

Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells

... Rabbit anti-succinyl cAMP serum, lyophilized. lysis reagent 1 lysis reagent 2 The above reagents are available in the cAMP &...

Aurum Plasmid 96 Kit Tech Note, Rev A

...eparing the plasmid, including alkaline lysis of the bacteria and alcohol precipitation of the D... Typically, plasmid purification kits use alkaline lysis followed by guanidinium salts to bind t...it offered the user the opportunity to perform the lysis without centrifuging the bacteria befor...

Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System

...Pellets were resuspended and incubated for 1.5 hours in 10 ml of lysis solution (0.15 M NaCl, 0.1 M Na 2 EDTA) containing 15 g/ml of f...mples followed by a 30 minute incubation at 37 C. Next, 10 ml of lysis buffer (0.1 M NaCl and 0.5 M Tris-HCl) was added and samples wer...

Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses

... in cell extracts prepared 24 hours after electroporation. Adherent cells were washed twice with cold PBS and lysed in 300 l luciferase lysis buffer. Extracts were centrifuged briefly to remove cell debris. Luciferase was quantitated using luciferin (Analytical Luminesc...

Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

...gene expressed from the cauliflower mosaic virus 35S promoter (Jefferson, et al., 1987). Both plasmids were prepared by alkaline lysis (Maniatis, et al., 1982) followed by cesium chloride purification and ethanol precipitation. Biolistic Transformation ...