Lysis

A Positive Selection Assay for Mutation Analysis in Big Blue Animals

Select-cII mutation assay kit Animals Stratagene Stratagene now offers an alternative way to perform mutational analysis inthe Big Blue<sup...

Epitope-Tagging Vectors for Functional Analysis in Yeast

Stratagene Stratagene has developed a series of epitope-tagging vectors to expressand functionally analyze eukaryotic genes in the budding yeast Saccharomyce...

Map and Link Human Genetic Disorders with SSLP Analysis

system Medical Genetics Branch, National Human Genome Research Institute NationalInstitutes of Health, Bethesda, MD system is idealfor analyzing SSLPs (simple sequence-lengt...

CastAway Precast Gels for Rapid Automated DNA Sequence Analysis

Stephen Roemer Mary Jensen Harry Osterman MarkVanek LI-COR Biotechnology Division LI-COR and Stratagene release the first commercially available precastgels for automated DNA sequencing and genetic analysis. Stratagenes CastAway gels* for...

Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System

Martin Simon, Petra Albrecht, Helmut J. Schmidt, and Andrs Jarrin Martin Simon, Petra Albrecht, and Helmut J. Schmidt, University of Kaiserslautern, Germany, Department of Ecology Andrs Jarrin, Eppendorf AG With the help of the Eppendorf cM...

High-throughput gene expression analysis to screen for anti-asthma drugs

Andreas Pahl, Uwe Oelmller,* and Susanne Ullmann Department of Pharmacology, University of Erlangen, Erlangen, Germany * QIAGEN GmbH, Hilden Germany Drug library screening based on gene expression analysis requires reliable and reproducible methods of RNA pu...

Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci

Marion Schmidt, Holger Engel, Markus Sprenger-Hauels, Corinna Kppers, Gaby Schulte, Silke Zobel, and Dirk Lffert QIAGEN GmbH, Hilden, Germany Microsatellites highly polymorphic DNA markers comprised of nucleotides that are repeated in tandem arrays and distributed throughout the geno...

Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn

Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MS Shiaw-Lin Wu, Pavel Bondarenko, Tom Shaler, Paul Shieh, and William S. Hancock The data presented here can be acquired using...

Amino Allyl Labeling for Array Analysis

RNA is often labeled with fluorescent dyes (i.e. Cy3 or Cy5) for microarray analysis. Label may be directly incorporated during cDNA synthesis, aRNA amplification or a post-aRNA reverse transcription (RT) reaction, or attached via a chemical coupling reaction performed after any enzymatic reactions...

Array Analysis: The Basics

Gene arrays are solid supports upon which a collection of gene-specific nucleic acids have been placed at defined locations, either by spotting or direct synthesis. In array analysis, a nucleic acid-containing sample is labeled and then allowed to hybridize with the gene-specific targets...

Northern Analysis: The Basics

<A href="#2">Steps > <A href="#3">RNA Isolation, Probe Generation <A href="#4">Electrophoresis, Transfer <A href="#5">Hybridization, Detection <A...

SeeSNP Propietary Analysis Advantages

Kevin McKernan, Jim Yang, Betty Woolf, Rey Sequerra, Kathy Makowski, Tom Tang Agencourt Bioscience Corporation, Beverly, MA 01915, USA Understanding genetic variation and its relationship to drug response and disease susceptibility is becoming an increasingly important consideration i...

DGGE Analysis Using the DCode System, Rev B

Bio-Rad Laboratories, Inc., Hercules, CA 94547 USA Introduction Denaturing gradient gel electrophoresis (DGGE) is one of several methods that can be used to screen DNA fragments for small sequence changes or point mutations. In DGGE, the denaturing environ...

DGGE Analysis for Non-Invasive Studies of Primate Diet

Jodi A. Irwin, Department of Cell and Molecular Biology, NorthwesternUniversity Medical School, 303 E. Chicago Ave., Chicago, Il 60611-3008j-irwin@nwu.edu; Cristin Orrego, Conservation Genetics Laboratory,Department of Biology, 1600 Holloway Avenue, San Francisco State University,San Francisco, CA 94132 cob@sfsu.edu Introduction <br...

Use of Transcriptor Reverse Transcriptase in Microarray Analysis

Researchers studying tissue and cellular differences in gene expressionhave applied microarray analysis to questions ranging from cancer researchto plant development studies. Although the method is well established, manyof the steps in the process continue to be optimised...

Increased Sensitivity in Microarray Analysis

Transcriptor Reverse Transcriptase inthe Eberwine Target Preparation Workflow Reproducibility and sensitivity are key parameters for thequality of a gene expression profiling experiment. Theimproved reliability of commercially avail...

Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS

In human cells, the enzymes thymidin phosphorylase(TP), dihydropyrimidine dehydrogenase (DPD), and thymidylatesynthase (TS) play a pivotal role in the metabolismof pyrimidines. In cancer disease medical care, thecytotoxic compound 5-fluorouracil (5-FU) is the standard...

Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining

A fast and accurate method to detect green fluorescent proteinexpression in samples of low cell numbers This Application Noteaddresses the major problemsthat many researchers workingwith primary cells have, for example,limited availability, quan...

Agilent 2100 Bioanalyzer Automated Analysis System

Powered by major advances in microfabricationtechnology, the developmentof a fully automated laboratory on a chipis now a reality. Designed to integratesample preparation, fluid handling andbiochemical analysis, "lab-on-a-chip"is revolutionizing life science resea...

2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer

Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer The Agilent 2100 bioanalyzer is aunique, on-chip, electrophoresis andflow cytometry analysis tool for RNA,DNA proteins and cells....

Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis

Whereas in the past mass spectrometers (MS) were confined to therealm of small molecules, the development of soft ionization methodssuch as matrix-assisted laser desorption/ionization (MALDI) and electrosprayionization (ESI) revolutionized the analysis of large biomolecule...

Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori

Gastric infection with Helicobacterpylori (H. pylori) is the majorcause of chronic active gastritisand is associated with the pathogenesisof peptic ulcer and gastriccarcinoma. Recent studies using amultiplex PCR (mPCR) basedapproach distinguished differental...

High resolution DNA analysis with the DNA 500 and DNA 1000 LabChip kits

Slab gel analysis is the traditionaltechnique used for DNA analysis.By comparing the separated bandsof an unknown sample to a knownstandard (size or mass ladder), thesize and quantitation of each fragmentcan be estimated. The Agilent2100 bioanalyzer is an instr...

Analysis of Microarray Data

and aRNA Samples Clustering analysis is commonly used for interpreting microarray data. It provides both a visual representation of complex data and a method for measuring similarity between experiments (gene ratios). The widely used methods for clustering microarray data are: Hierarchical, K-means and...

aRNA Synthesis and Labeling for Array Analysis

Amino Allyl MessageAmp II aRNA Amplification Kits-- Now Available with Cy Dyes ArrayScript Reverse Transcriptase--high yields of full-length cDNA increases aRNA yields MEGAscript transcription technology-- up to...

Bacterial Whole Genome Array Analysis

The rapid increase in the number of bacterial genomes sequenced over the past several years has opened up a new era in whole-genome expression analysis using DNA microarray te...

Improve RNA Isolation for Array Analysis and More

Researchers performing array analysis have found that a combination of phenol based and solid phase RNA extraction methods often provide superior array data than either method alone (1-3). Ambion's new line...

New Tools for Small RNA Analysis

Non-coding small RNAs such as transfer RNAs (tRNA), ribosomal RNAs (rRNA), small nucleolar RNAs (snoRNA), and small nuclear RNAs (snRNA) play critical roles in a variety of cellular processes, from regulation of mRNA translation, pre-mRNA splicing or transcription to chromosome...

Perform Microarray Analysis with Limited Bacterial RNA

Linear RNA amplification technology for bacterial gene expression analyses As little as 10 ng of mRNA or 100 ng total RNA required for array analysis Low input requirements enable analysis of mixed host-pathogen samples Ful...

MessageAmp II: Use Less RNA for Array Analysis

Amplifies as little as 100 ng RNA in a single round High Percent Present Calls and low 3'/5' ratios in GeneChip analysis Includes ArrayScript M-MLV RT engineered to maximize yields of full-length cDNA The best in vitro transcr...

Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis

RNAqueous-Micro Kit Ideal for Processing Laser Capture Microdissection Samples Further development of Ambion's RNAqueous-Micro Kit has resulted in a 3-6 fold increase in total RNA yields from LCM samples. In addition, Ambion has introduced a new LCM Staining Kit that has been o...

MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

Jaclyn Shingara, Kerri Keiger, Ila Wolf, Jeffrey Shelton, Emmanuel Labourier, and David Brown R&D Division, Ambion, Inc. MicroRNAs (miRNAs) are small, siRNA-like molecules, encoded in the genomes of plants and animals,...

High Throughput RNA Isolation for High Performance Microarray Analysis

Protocol combines benefits of TRI Reagent sample lysis with MagMAX magnetic bead-based RNA isolation technology Procedure is compatible with fresh, flash-frozen, or RNAlater-treated tissues...

Measuring RNase Activity A Real-time Kinetic Analysis

The RNaseAlert QC System, when used in conjunction with a fluorometer capable of kinetic analysis, provides a rapid and ultra-sensitive assay for determining RNase A specific activity. The RNaseAlert QC System also detects a variety of other nucleases, including RNase A, T1, RNase I, micrococcal nuclease, S1 nuclease,...

Rapidly Prepare Probes and Markers for Small RNA Analysis

Ideal for analyzing miRNA, siRNA, and other small RNAs Prepare high specific activity RNA or DNA probes Contains reagents to prepare Decade Markers and single-base </B...

RNA Amplification for Array Analysis

A. How is fold amplification calculated? RNA amplification using the Van Gelder and Eberwine technique (Van Gelder 1990) utilizes an oligo(dT) primer containing the T7 RNA polymerase promoter for synthesis of first strand cDNA. The po...

RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis

Tissue Collection: RNA Stabilization Solution is the worldwide standard for the preservation of RNA in intact animal tissues, organs, and cells. RNA eliminates the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later proces...

RNAi for High Throughput Analysis

Large scale gene expression studies require reliable high-throughput methods to synthesize and then purify high quality RNA from a large number of samples. Although the MEGAscript RNAi Kit includes reagents for both dsRNA production and clean up, it is not optimized for clean up in a 96 well plate forma...

RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs

(Senior Scientist, Ambion, Inc.) The realization that RNA interference (RNAi) can be used as a general tool for mammalian gene function studies occurred with the seminal Elbashir et al. (1) publication describing the use of small interfering...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

Mike Byrom, Vince Pallotta, David Brown*, and Lance Ford* Ambion, Inc. * Corresponding authors <A href="mailto:dbrown@ambion.com">dbrown@ambion.com , <A href="mailto:lford@ambion.com">lford@ambion.com...