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Luminescence in Biological Technology

Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)

Jinfang Liao, M.D., Ph.D. and Evelyn McGown, Ph.D. Molecular Devices Corporation, 8/00 INTRODUCTION The photoprotein aequorin, a bioluminescent protein from the coelentratejellyfish Aequorea victorea, has proven to be highly sensitive to the trace amou...

Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

Abstract The microplate format has become a standard for many biological assays such as high throughput screening for drug discovery and radioimmunoassays. Productivity with these high volume procedures has been limited by the amount of sample handling required and low sample...

Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter

Abstract The TopCount Microplate Scintillation and Luminescence Counter has been widely used in biopharmaceutical assays involving both radionuclide and luminescence measurement. A new feature allows the use of TopCount for measuring a high energy beta emitter such as 32 P b...

Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System

Key words: luminescence • BRET 2 • LEADseeker • DeepBlueC• cell-based assays Competitive pressure is forcing organizations to continually increase screening throughput. Yet accuracy and sensitivity remain vital concerns, par...

Time gated separation of lanthanide luminescence

Introduction The distinctive luminescence of lanthanide ions has long been a challenge for the spectroscopist. Methods to counter the extremely low absorption coefficients and luminescence quantum yields have concentrated on Protective ligands to redu...

ForteBio Launches New Assay Capabilities for High-Sensitivity Protein Quantitation and Fragment Screening on Its Octet(R) Platform

...w nanogram per mL levels of analytes in quantitation mode. "For protein quantitation, life science researchers have traditionally run fluorescence and luminescence ELISA methods to achieve greater sensitivity in their assays. Now, using ForteBio's new modules, they can obtain highly precise results within an hour...

Odyssey Thera Granted U.S. Patent for Animal Imaging

...ith a single system that faithfully monitors the activity of a drug at each step." Dr. Westwick continues, "Whole animal studies using fluorescence or luminescence detection are widely used in the pharmaceutical industry, but strategies were needed to increase the scope of biochemical activities that can be track...

'High Q' NIST nanowires may be practical oscillators

... who grew the nanowires. NIST has developed a unique way of growing hexagonal gallium nitride (GaN) nanowires featuring low defect density and high luminescence intensity. In a new paper*, researchers at NIST and the University of Colorado at Boulder report high Q factors in wires that are 30 to 500 nanometers...

Kodak in vivo imaging system: precise coregistration of molecular imaging with anatomical X-ray imaging in animals

... In-Vivo F and the Kodak Image Station In-Vivo FX. The In-Vivo F allows for very high resolution, multi-wavelength fluorescence, luminescence and radioisotopic imaging in small animals. The In-Vivo FX includes all of the capabilities of the In-Vivo F and the high-resolu...

Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar

...RAstar (figure 1) BMG LABTECH PHERAstar luminescence optic module ...uator at a final concentration of 60 M, luminescence reporter signal was recorded, followed by ...ion of the CellTiter-Glo reagent by the Equator. luminescence was recorded a second time to measure A...

Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar

... and cell viability Monitoring of both luminescence and fluorescence output signals from on...PHERAstar microplate reader is used to record both luminescence and fluorescence, depending on the mult...RAstar (figure 1) BMG LABTECH PHERAstar luminescence optic module BMG LABTECH PHERAstar fluo...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

...e that the addition of luciferin/luciferase reagent and subsequent read can be automated so that variability due to differences in timing of the flash luminescence reaction can be eliminated. REFERENCES 1. McGown, E.L. 2000. UV Absorbance Measurements of DNA in Microplates. BioT...

Measuring siRNA-mediated knockdown of IL-8 mRNA

...re shown in Figure 2. Coefficients of variation (CVs) measured from different treatments ranged from 5 to 15% over a 3-log range of relative luminescence (RLU) values. These CVs allow for the clear distinction of the effects of the siRNA seen at four hours (89% knockdown) from the effects seen at eight ...

Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

...s variance is much larger than other sources of error. For details, see Ingle, J.D and S.R. Crouch. 1988. Signal-to-noise expressions for emission and luminescence measurements. In Spectrochemical Analysis. Prentice Hall, Inglewood Cliffs, NJ. pp. 146-150. back to top ...

Evaluation of FlashPlate in a Helicase Assay

...lusively from PerkinElmer Life Sciences. The platform is compatible with automated assay equipment such as the TopCount Microplate Scintillation and luminescence Counter from Packard Instrument Company. Materials Streptavidin FlashPlate PLUS (SMP103) Oligonucleotides ...

A highly sensitive ATP-luminescence assay using VICTOR2 with the dispenser option

...DUCTION In high sensitivity flash type luminescence assays the measurement is done just after dispensi...t or after a short delay time. Over the past years luminescence substrates have been improved towards providing mo...e one advantage, sensitivity. In the glow type the luminescence signal is spread over a wide time scale (for examp...

FREE FAT CELL ASSAY

...nt of radio labelled glucose, are negligible. Previously, the scintillators that were available for use in the TopCount microplate scintillation and luminescence counter did not have the preferred characteristics for the phase separation, so the toluene scintillator method, with its many undesirable chemical an...

Cyclodextrin enhances room temperature phosphorescence

...ty has led to an important use of cyclodextrins in luminescence spectroscopy. Cyclodextrins have been found to enh...P >

Figure 2 shows the luminescence spectra for phenanthrene included in β-CD, wi...n inner diameter of 0.78 nm. Figure 3 compares the luminescence spectra for the same solution of phenanthrene and ...

Phosphorescence lifetime measured using the SPEX phosphorimeter

...cept as noted. The scans were taken under ambient room conditions.

Results and Discussion Figure 1 compares luminescence from the peptide- Tb complex with no delay and a 50-s delay following the excitation light pulse. With the delay, the fluorescence at 363 nm fro...

Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP

...is a multi-detection system, capable of measuring absorbance (Vis and UV), prompt fluorescence, fluorescence polarization, time-resolved fluorescence, luminescence and AlphaScreen reagents in 96-, 384- and, in most modes, 1536-well plates, with a single detector. The following study was conducted to compare the p...

Direct Counting of Millipore MultiScreen Filtration Plates.

... Abstract The TopCount Microplate Scintillation and luminescence Counter can be used to count samples prepared in MultiScreen microplates. These plates are designed so that all assay steps are performed in a single ...

Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor

...n was stopped by aspirating the contents of each well. The FlashPlate was then immediately placed on a Packard TopCount Microplate Scintillation and luminescence Counter to measure bound radioactivity. Results Several experiments were performed to determine the optimum conditions for bindi...

Counting Cytostar-T scintillating microplates on the MicroBeta JET

...rku, Finland) INTRODUCTION MicroBeta JET is a multi-detector instrument designed for liquid scintillation (LS) or luminescence detection of samples in microtitration plates, tubes or on filters. Three different MicroBeta models are available. Two manually loaded mode...

Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System

... concerns, particularly for common screens such as luminescence assays. LEADseeker™ Multimodality Imaging Sy...modality Imaging System equipped with the supplied luminescence filter. Used together, the components support orga...> Other materials required luminescence 660 nm cut-off filter 384-w...

Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3', 5' monophosphate and its validation for pharmacological screening at G protein-coupled receptors

...oone Lumitrac 200 1536-well microplates and measured on the LEADseeker Multimodality Imaging System (2 ×2 binning with coincidence averaging) in luminescence mode (Fig 1a). The assay had a comparable limit of detection and working range as a model cAMP scintillation proximity assay (SPA) (RPA559) also carri...

Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses

...se lysis buffer. Extracts were centrifuged briefly to remove cell debris. Luciferase was quantitated using luciferin (Analytical luminescence Laboratory, San Diego) employed according to the manufacturers protocol, collecting the light produced by 20l of extract for 10 ...
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