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Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
Stratagene Dartmouth School of Medicine, Dept. of Microbiology, Hanover, NH</p...Imaging Chemiluminescent Western Blots
II still video system to directly acquireimages from chemiluminescent blots Stratagene II still video system is well suited for directimaging of chemiluminescent Western blots. The light-tight cabinet is ideal forthe longer exposure times necessary to...A highly sensitive ATP-luminescence assay using VICTOR2 with the dispenser option
In high sensitivity flash type luminescence assays the measurement is done just after dispensing the activating reagent or after a short delay time. Over the past years luminescence substrates have been improved towards providing more stable signal. These so-called glow type substrates allow microplates to be counted without dispensors. Ho...Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter
The microplate format has become a standard for many biological assays such as high throughput screening for drug discovery and radioimmunoassays. Productivity with these high volume procedures has been limited by the amount of sample handling required and low sample counting throughput. Now, however, microplate assays can be efficiently count...Jinfang Liao, M.D., Ph.D. and Evelyn McGown, Ph.D. Molecular Devices Corporation, 8/00 The photoprotein aequorin, a bioluminescent protein from the coelentratejellyfish Aequorea victorea, has proven to be highly sensitive to the trace amount of Ca in cells. The aequorin complex which contains the...
ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)
Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Adenosine triphosphate (ATP) is present in all living cells. Because the level isstrictly-controlled, assay of ATP can be used as a indicator of viable cell number.ATP measurements are used to monitor raw materials and manufacturing...DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)
Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 DNA measurements are important for many aspects of molecular biology. Of thespectrophotometric methods available, UV absorbance measurement at 260 nm isthe simplest and has recently gained widespread popularity in the microplateforma...Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Immunoassays have traditionally employed colorimetric indicators. As such, multiple dilutions of samples were necessary to ensure that they fell within the dynamic range of the assay. Recently, chemiluminescent immunoassays, with gre...
Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)
Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Reporter gene assays are used to study eukaryotic gene expression. Dual geneticreporters are commonly used in transient transfections of cultured cells tominimize experimental variability caused by differences in cell number, viabili...Evelyn McGown, Ph.D. , Michael Su, M.S. and Jin Liao, M.D., Ph.D. Molecular Devices Corporation, 8/00 Luciferase from the North American firefly (Photinus pyralis) is very popular foruse in Reporter Gene Assays. The enzyme catalyzes the ATP-dependent oxidationof luciferin with the concomitant release of light...
Quantitative determination of DNA synthesis in cellcultures is now a routine procedure in many laboratories.Protocols are available for various applications,especially in cell culture systems. The effects of growthfactors, inhibition of cell division by exogenous factor...
AMARA • kinase assay • LEADseeker • chemiluminescence It is predicted that at least 500 kinase and phosphatase enzymes exist, their activities having wide-ranging regulatory responses. Many kinases are part of a signaling cascade in disease pathways such as inflammation and cancer, so identifying modulators of kinase...
<P >J. Turner GE Healthcare, The Maynard Centre, Cardiff, UK Competitive binding assays have been developed for measuring the functional activity of a range of serine-threonine and tyrosine kinases. Based on enzyme fragment complementation and the subsequent hydrolysis of chemiluminescent substrate, HitHunter™ Kinase Assay Kits gene...
Competitive pressure is forcing organizations to continually increase screening throughput. Yet accuracy and sensitivity remain vital concerns, particularly for common screens such as luminescence assays. LEADseeker™ Multimodality Imaging System was de...
Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System
Competitive pressure is forcing organizations to continually increase screening throughput. Yet accuracy and sensitivity remain vital concerns, particularly for common screens such as luminescence cell-based ass...Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
P by Cerenkov counting, a well-known measurement technique that has the...Photoluminescence Spectroscopy of Quantum Dots, monitored by the Spex FluoroMax
The optical properties of quantum dots (QDs) have potential applications in optoelectronics, biosensing and biolabeling, memory devices, and sources of laser light. For example, alloyed CdSeTe QDs are shown herein to possess a nonlinear change in their photoluminescence spectra, correlated to size and composition, as monitor...Time gated separation of lanthanide luminescence
The distinctive luminescence of lanthanide ions has long been a challenge for the spectroscopist. Methods to counter the extremely low absorption coefficients and luminescence quantum yields have concentrated on Protective ligands to reduce vibrational quenching effects by XH moieties (X = O, N,)....Alien expert illuminates audience on extraterrestrial possibilities
MADISON - Some of extraterrestrial expert s beliefs may leave people bewildered. Speaking to an audience of 300 at the University of Wisconsin-Madison Feb. 19, Drake, director of the Search for Extraterrestrial Intelligence Institute in Mountain View, Calif., explained the reasons why he believes in...Stem Cells Illuminate Early Stages of Human Development
MADISON - When introduced to the world in 1998, human embryonic stem cells were considered heralds of a new age of transplant medicine. The prospect of an unlimited supply of cells and tissue of all kinds to treat disease captured public imagination and enthusiasm. But lost in the glitz of the cells' potential to treat an array of devastating and sometimes fatal diseases was another qual...Other TagsGemGemGemGemGemGemSuffererOther Contentsfrostbitefrontalfrontalfrontalfrontalfrontalfunnelfunnelfurniturefurniturefurniturefurniturefurunclegalactosegalactose
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(Date:12/1/2009)...ne from the United States, the other from Canada, ...his wolf pack, on remote Ellesmere Island, only 60...y find out what these "North Pole wolves" do in th... areas of the world., "We first encountered 9-ye...nowned U.S. Geological Survey (USGS) wolf research...
(Date:12/1/2009)...h University-led international research team has d...the next generation of solar cells. , Scientists...agues from the universities of Wollongong and Ulm ...lar cells with a three-fold increase in energy con...ed tandem dye-sensitised solar cells. , Lead re...
(Date:12/1/2009)...r listening to the radio anymore. They,re used in ...ch from North Carolina State University is revolut...pe-shifting antennas that open the door to a host ...o military deployment. , Modern antennas are mad...ations to how far they can be bent and how often ...
Breaking Biology News(10 mins):North Pole wolf emails locations to researchers 2North Pole wolf emails locations to researchers 3Innovation puts next-generation solar cells on the horizon 2Shape shifters: Researchers create new breed of antennas 2If Hearing Loss is Not Treated Brain Can Forget How to Hear and Understand Speech 59746 1If Hearing Loss is Not Treated Brain Can Forget How to Hear and Understand Speech 59746 2IPRO Wins Federal Contract to Support Hospice Oversight 59743 1IPRO Wins Federal Contract to Support Hospice Oversight 59743 2Allied Worlds Susan Chmieleski to Speak at American Society for Healthcare Risk Management 28ASHRM 29 Annual Conference and Exhibition 59740 1Allied Worlds Susan Chmieleski to Speak at American Society for Healthcare Risk Management 28ASHRM 29 Annual Conference and Exhibition 59740 2Allied Worlds Susan Chmieleski to Speak at American Society for Healthcare Risk Management 28ASHRM 29 Annual Conference and Exhibition 59740 3(Date:12/1/2009)...es H1N1 vaccination because pandemic,s course rema...ealthDay News) -- A U.S. health official said Tues... the wane nationally, but many experts agreed the ..., The flu is now widespread in 32 states -- down f...en, director of the U.S. Centers for Disease Contr...
(Date:12/1/2009)... , RESEARCH TRIANGLE PARK, N.C., Dec. 1 /PRNe...ated in 2001 and have expanded over the years serv.... The facility which was originally set up in an a...acy services, as part of the suite of services by ...dustry as a valuable offering. ,, The pharmacy ...
(Date:12/1/2009)..., WASHINGTON, Dec. 1 ... for International Development: ,, Chairman Ker...ttee. ,, I am honored to be nominated by Presid...States Agency for International Development, and I...y. ,, Secretary of State Clinton has expressed...
(Date:12/1/2009)...mber 1, 2009) The ADEAGies Foundation will honor ...ent Award at the Gies Awards celebration to be hel...unction with the 2010 ADEA Annual Session & Exhibi...ation, and Achievement recognize contributions to,...nitiatives. For a complete listing of Gies Award w...
(Date:12/1/2009)...r cardiovascular system tied to academic achieveme...thDay News) -- A strong cardiovascular system in y...tter school grades and more overall success later ...st doctors and laypeople know (or should know) the...dies, the authors of a new study, appearing in thi...
Breaking Medicine News(10 mins):Health News:Swine Flu Waning, But Could Return 2Health News:Swine Flu Waning, But Could Return 3Health News:Testimony Before the Senate Committee on Foreign Relations Administrator Designate Dr. Rajiv Shah December 1, 2009 2Health News:Testimony Before the Senate Committee on Foreign Relations Administrator Designate Dr. Rajiv Shah December 1, 2009 3Health News:Testimony Before the Senate Committee on Foreign Relations Administrator Designate Dr. Rajiv Shah December 1, 2009 4Health News:Testimony Before the Senate Committee on Foreign Relations Administrator Designate Dr. Rajiv Shah December 1, 2009 5Health News:Testimony Before the Senate Committee on Foreign Relations Administrator Designate Dr. Rajiv Shah December 1, 2009 6Health News:Testimony Before the Senate Committee on Foreign Relations Administrator Designate Dr. Rajiv Shah December 1, 2009 7Health News:Testimony Before the Senate Committee on Foreign Relations Administrator Designate Dr. Rajiv Shah December 1, 2009 8Health News:Testimony Before the Senate Committee on Foreign Relations Administrator Designate Dr. Rajiv Shah December 1, 2009 9Health News:The Forsyth Institute receives the William J. Gies Award for outstanding achievement 2Health News:Exercise May Lead to a Smarter, More Successful You 2Health News:Exercise May Lead to a Smarter, More Successful You 3[0] Swine Flu Waning, But Could Return [0] Max Neeman International Expands Its Pharmacy Services Facility In Delhi[0] Testimony Before the Senate Committee on Foreign Relations Administrator Designate Dr. Rajiv Shah December 1, 2009[0] The Forsyth Institute receives the William J. Gies Award for outstanding achievement[0] Exercise May Lead to a Smarter, More Successful You
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