Liver

pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins

Faster, easier screening for difficult transfections when using thecomplete control kit Stratagene We describe the vector pFB-ERV, an MMLV-based replication-defectiveretroviral vector for delivery of the ecdysone receptor proteins RXR and VgEcR.In stable cell lines infected with an estima...

High-Titer Retroviral Vectors for Gene Delivery

High-TiterRetroviral Vectors for Gene Delivery Stratagene The high-titer retroviral vectors pFB and pFB-Neo can be used with anyMoloney Murine L...

Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance

Improve reliability of PCR reactions with Herculase enhanced DNApolymerase Stratagene enhanced DNApolymerase,* a novel enzyme formulation that providessuperior performance in both routine and demanding PCR applications. Previous...

An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat

GBF Gesellschaft fr Biotechnologische Forschung mbH (Society for Biotechnological Research) DNA microarrays are a key technology in modern molecular biotechnology. Due to their high degree of parallelism, they allow the...

Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures

Jochen Meier, Ivonne Strmel, Radu Iosub, Sonja Schmidt, and Rosemarie Grantyn Developmental Physiology, Johannes Mller Institute, Humboldt University Medical School (Charit), Berlin, Germany Efficient delivery of DNA to primary neuronal cell cultures is of critical importance...

UW professors get grants to engage technology for course delivery

Cramer, professor Hussain Bahia, program assistant Carole Kraak and staff from Engineering M...

A New Way to Assess siRNA Delivery Efficiency

Assess GAPDH siRNA delivery in less time for a fraction of the cost of real-time PCR Measure both GAPDH siRNA-induced knockdown AND transfection-induced toxicity Use with Silencer GAPDH siRNA Controls and Silencer CellReady Transfecti...

Delivering siRNA Using Adenoviral Vectors

Efficiently deliver an siRNA expression construct into a variety of mammalian cells and organisms Ideal for many primary and other difficult to transfect cells Positive Control Oligonucleotide Insert an...

Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection

(Research Associate II, Ambion, Inc.) Gene silencing using small interfering RNAs (siRNAs) has become a powerful method for studying gene function. Human primary cells are often desired for such experiments because they are mor...

Delivering siRNAs to Difficult Cell Types

Dmitriy Ovcharenko, Rich Jarvis, Kevin Kelnar, David Brown, Ambion, Inc. RNA interference (RNAi) is a powerful experimental tool for reducing the expression of specific genes. It...

Enhanced siRNA Delivery and Long-term Gene Silencing

5.1 Retro System enables researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. These retroviral vectors make use of H1 or U6 Polymerase III promoters...

Deliver siRNAs Into Primary Cells

Deliver siRNA into primary cells, cells grown in suspension, and other difficult-to-transfect cells Maintain high cell viability under a wide range of conditions Optimize electroporation of your...

High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells

A variety of siRNA applications, including siRNA library screening, require the efficient delivery of hundreds to thousands of siRNAs to cells. Most published siRNA studies describe performing transfections in 6 well and 24 well formats. Ambion has developed two reagents, siPORT...

Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice

Andrew Conn, Lindy Durrant, and Ian Spendlove, Cancer ResearchCampaign Academic Unit, Nottingham City Hospital, Hucknall Road,Nottingham NG5 1PB, UK Gene gun immunization through the skin is a reliable and reproducible method of DNA vaccine delivery, and has been shown to be capable of inducing...

Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system

adenoviral vector • viral transduction • EGFP • IN Cell Analyzer • gene delivery There is a requirement in the drug discovery process to investigate lead candidate drugs arising from primary screens and to validate those compounds in suitable biological assays. There is also a need in early-stage drug discov...

Is Z' factor the best assessment for the quality of cellular assays delivering higher content?

Amersham Biosciences UK Limited, Amersham Place, Little Chalfont, Buckinghamshire HP7 9NA England As high throughput imaging systems, analysis al...

Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system

adenoviral vector • viral transduction • EGFP • IN Cell Analyzer • gene delivery Biological assays developed in cultured and primary cells can greatly aid secondary screening as well as lead and target validation, but their development presents several challenges. Establishing stable cell systems to express...

Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system

adenoviral vector ● viral transduction ● NTR ● IN Cell Analyzer ● gene delivery Biological assays developed in cultured and primary cells can greatly aid secondary screening as well as lead and target validation, but their development presents several challenges. Establishing stable cell systems to expre...

Delivering value instead of advertising - what a concept!

We find that families do not want to have their...

DHC 2007: Hayden says technology creates magic moment for healthcare delivery

, described the progress Wisconsin is making to advance the use of health information technology, particularly <a href="http://ccbh...

Agile Revolution: A new era of software delivery

Returning value to IT requires shedding the flawed processes of yesterday, and introducing methods that create greater alignment between IT and...

Mirus Bio awarded patent on RNA delivery

The company has been working to address the lack of an effective method to deliver nucleic acids such as RNA for the study of gene function, and along wi...

Will technology improve healthcare delivery?

We have the opportunity to transform health care and improve patient safety by better leveraging information technology to improve the efficiency, accuracy, and effectiveness of our healthcare system. But adoption has been slow, and the results have been mixed. If deployed incorrectly, without well conceived process improvements, IT systems can do just the reverse,...

Delivery of needles in the Capitol missed the point about stem-cell research

If Churchill was around to describe the July 19 delivery of 1,400 used syringes to the Capitol o...

ProHealth Care selects NetScaler delivery system

ProHealthCare selected the system to better coordinate application usage and response time acros...

Esker awarded patent for DeliveryWare Rules Engine

DeliveryWare automatically recognizes and extracts data from electronic documents to determ...

Berbee puts people first to deliver high-quality technology

Now in its twelfth year, Berbee has channeled these philosophies to create one of the largest and most versatile information technology providers in the Midwe...

Drug-delivery patch wins at UW business-plan competition

Anthony Esca...

Text ads deliver what you pay for - and more

The study, sponsored by Ask Jeeves, Google, ING, Lycos Search and Overture, shows that sponsored text advertising in the search environment works on two levels: paid click-thrus from active sho...

The challenges of maintaining patient privacy and delivering secure clinical information

MADISON The white board in the middle of the emergency room that lists each patient and their condition is a familiar scene in many hospitals. But is it a violation of patient privacy? Balancing the best interest of the patient but still providing information to the many care givers that require it was the top...

Merge to utilize Eskers technology to automate delivery of radiology reports

MADISON - s DeliveryWare Platform in its Fusion RIS system to automate faxing of radiologists reports, eliminating the need to print out and manually fax them. The system will send reports to referring physicia...

Wisconsin startup delivers project management relief to creative firms and ad agencies

Green Bay, Wis. - The system will allow logins with adjustable permissions from designers, project managers and clients. It is intended as a central solution for keeping track o...

Third Wave Achieves Milestone, Delivers 200,000 SNP Assays to RIKEN In Japan

MADISON, Wis. -- Since the beginning of 2003, Third Wave has been supplying its Invader SNP assays to Dr. Yusuke Nakamura, group directo...