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Counting Colonies or Plaques Directly from Images of Agar Plates

Michelle Cayouette Jane Moores Michael Steege AlanOrr Stratagene Cloning Systems, Inc. software version 3.1,researchers have the ability to automatically count bacterial colonies andplaques on agar plates. Researchers can use...

Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection

total RNA microprep kit for very small samples Stratagene Isolating total RNA from laser capture microdissection samp...

New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways

Stratagene cis-reportingsystems now includes the GAS and ISRE reporting systems, to better study theactivation of signal transduction pathways stimulated by int...

Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit

Gel Cleanup Kit George Halley, Eppendorf 5 Prime, Inc. Agarose gel electrophoresis is used extensively to separate and analyze DNA that is subsequently used for downstream applic...

An Assessment of the Eppendorf twin.tec plate performance in PCR* applications

M. Arun Kumar, PhD, and Vincent Prezioso, PhD, Brinkmann BioSystems Laboratory To evaluate the performances of Eppendorf 96-well twin.tec PCR plates, we carried out PCR reactions with twin.tec plates and PCR microplates from another commercial mi...

TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates

to Amplify Long gDNA Templates Eppendorf 5 Prime, Boulder, CO, USA Eppendorf provides a complete system for long-range PCR1 analysis of gDNA. The Perfect gDNA Bloo...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

Eppendorf 5 Prime, Boulder, CO, USA The polymerase chain reaction (PCR*) is a powerful and sensitive technique used by scientists to amplify large quantities of DNA. Although the reaction mix and thermal cycling conditi...

Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn

Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MS Shiaw-Lin Wu, Pavel Bondarenko, Tom Shaler, Paul Shieh, and William S. Hancock The data presented here can be acquired using...

RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater

is a tissue storage solution that stabilizes and protects cellular RNA in fresh tissues. (Ambion's new RNA -ICE is designed specifically for use with frozen tissues - see "<A href="http://www.ambion.com/techlib/tn/103/1.html">Stop Grinding...

Genomic DNA Preparation from RNAlater Preserved Tissues

The following protocol is suitable for 100 mg tissue samples. Mouse kidney, lung, and spleen genomic DNA have been successfully extracted with approximate yields of 70 g DNA per 100 mg tissue....

Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A

Joseph W. Turek and Guy C. Le Breton, Department of Pharmacology,University of Illinois at Chicago, College of Medicine, Chicago, IL 60612.Address correspondence to: Joseph W. Turek, 835 South Wolcott, Departmentof Pharmacology M/C 868, University of Illinois at Chicago, Chicago, IL 60612.E-mail: jturek1@icarus.uic.edu Introduction...

Evaluation of FlashPlate in a Helicase Assay

P]-γ-ATP. The oligos wer...

Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

The microplate format has become a standard for many biological assays such as high throughput screening for drug discovery and radioimmunoassays. Productivity with these high volume procedures has been limited by the amount of sample handling required and low sample counting throughput. Now, however, microplate assays can be efficiently count...

Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers

Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between synthesis and degradation. Whereas prot...

Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

Evelyn McGown, Ph.D. and Anna Lam, B.S. Molecular Devices Corporation, 8/99 Modern assays for proteolytic activity typically use synthetic chromogenic or flu-orogenic peptide substrates. Enzyme selectivity is obtained by choosing a peptide sequence uniquely recognized by the catalytic site of a particular enzy...

LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)

Anne T. Ferguson, Ph.D. Molecular Devices Corporation This application note describes the use of the SPECTRAmax Viability/Cytoxicity Assay Kit for animal cells. This assay uses two fluorescent dyes, calcein AM (cal...

Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)

By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the NanoOrange Pro software from Mo...

Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

By Yan Zhang., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the OliGreen Pro software from...

PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

Protein concentration is commonly estimated by measuring the absorbance at 280 nm and calculating the concentration using the extinction coefficient of the protein. The procedure is feasible because extinction coefficients are typically obtained using standard cuvettes with a fixed 1 cm pathlength. Extinction-based protein assays can also...

Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)

Most spectrophotometric methods for inorganic phosphorus (Pi) analysis are based on the color formed upon the reduction of a phosphomolybdate complex. The classic (and still widely-used) method is that of Fiske and Subbarow , in which the reduction is carried out with sulfite and aminonaphtholsulfonic acid. Over the years, modi...

UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)

Molecular Devices Corporation. 7/99 In 1994, Molecular Devices introduced SPECTRAmax , the first microplate reader equipped with a monochromator, rather than bandpass filters, for wavelength selection. It was also the first microplate reader capable of measuring absorbance below 340 nm. These new features transformed the micropl...

Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)

Jinfang Liao, M.D., Ph.D. and Evelyn McGown, Ph.D. Molecular Devices Corporation, 8/00 The photoprotein aequorin, a bioluminescent protein from the coelentratejellyfish Aequorea victorea, has proven to be highly sensitive to the trace amount of Ca in cells. The aequorin complex which contains the...

ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Adenosine triphosphate (ATP) is present in all living cells. Because the level isstrictly-controlled, assay of ATP can be used as a indicator of viable cell number.ATP measurements are used to monitor raw materials and manufacturing...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 DNA measurements are important for many aspects of molecular biology. Of thespectrophotometric methods available, UV absorbance measurement at 260 nm isthe simplest and has recently gained widespread popularity in the microplateforma...

High Sensitivity, Wide Dynamic Range, Horseradish Peroxidase (HRP) ELISA Using the LMax Microplate Luminometer (MaxLine Application Note #41)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Immunoassays have traditionally employed colorimetric indicators. As such, multiple dilutions of samples were necessary to ensure that they fell within the dynamic range of the assay. Recently, chemiluminescent immunoassays, with gre...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Reporter gene assays are used to study eukaryotic gene expression. Dual geneticreporters are commonly used in transient transfections of cultured cells tominimize experimental variability caused by differences in cell number, viabili...

Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)

Evelyn McGown, Ph.D. , Michael Su, M.S. and Jin Liao, M.D., Ph.D. Molecular Devices Corporation, 8/00 Luciferase from the North American firefly (Photinus pyralis) is very popular foruse in Reporter Gene Assays. The enzyme catalyzes the ATP-dependent oxidationof luciferin with the concomitant release of light...

96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation

Initially, work in 96 well microplat...

Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing

Applied Science and Technology Department, Caliper Life Sciences, Inc., Hopkinton, MA 01748 The High Volume Head is one of four multi-channel liquid handling options available for the Sciclone Advanced Liquid Handler (ALH). The architecture of the device includes an arra...

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

The TempliPhi DNA Sequencing Template Amplification Kit is designed to eliminate...

New MailFoundry product fights latest spam techniques

and <a href="http://www.tech-faq.com/brute-force-attack.sht...

Expand Long Template PCR System

for amplification of 520 kb targets from eukaryotic genomes Cat. No. 1 681 834 100 units Cat. No. 1 681 842 2 x 250 units Cat. No. 1 759 060 10 x 250 units Unique enzyme blend of Taq and Pwo DNA Polymerase. The system also includes PCR buffers f...

High Pure PCR Template Preparation Kit

For isolating genomic nucleic acids for PCR, restriction enzyme analysis, and Southern blotting Catalog Number Pack Size 1796828 1 kit (100 purifications)...

Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations

The endopeptidase originally isolated from the filtrate ofthe lower fungus Tritirachium album limber is recombinantlyexpressed in Pichia pastoris. Proteinase K isextremely effective on native proteins from differentsources, and remains active in the presence of denaturingreag...

Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA

This Application Note describes how Gene Logic, Inc., has improved itsability to analyze the quality of total RNA and fragmented cRNA by usingthe Agilent 2100 bioanalyzer. The Eukaryote Total RNA Nano and Picoassays are currently being utilized to examine the 28S/18S ratio...

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

Lucia Martinez and Ario de Marco EMBL Protein Expression Core Facility, Meyerhofstr. 1, D-69117, Heidelberg, Germany Immobilized Metal ion Affinity Chromatography (IMAC) is an easy and reliable technique that allows the fast separation of His-tagged proteins from total lysates. There are several commercially available resins but all of them s...

measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

Cathy Olsen, Ph.D., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089....

A Better Way to Isolate RNA AND Protein From the Same Sample

Isolate nuclear and cytoplasmic RNA and protein from cultured cells Ambion's new PARIS Kit uses a fast, simple procedure based on Ambion's RNAqueous technology to isolate bo...

Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform

Ambion has developed a complete line of research tools for the analysis of microRNAs (miRNAs). Recently, we introduced the Vana miRNA microarray platform (consisting of the Vana miRNA Labeling Kit and the Vana miRNA Probe Set) and several miRNA tools for interrogating miRNA function and identifyin...

Isolate It All: siRNA miRNA Total RNA Native Protein

, Ambion, Inc Isolate total RNA and native protein from the same sample Enrich for small RNAs ( Ideal for assessing siRNA or miRNA expression and processing Small interfering R...