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Lac in Biological Technology

Fond du Lac County tracks cell phones in emergencies

Fond du Lac County can now pinpoint the locations of cell-phone users when they call 911, making it just the second county in Wisconsin to install the necessary equipment. The system has been in place for about six weeks and has already been field-tested, according to The Reporter . In one c...

DNA2.0 Introduces Freedom of Expression With a New Family of Bacterial Expression Vect:ors

...eins at high levels and have been shown to express better than comparable pET vectors. pJexpress vectors carry a copy of the lacI gene and symmetrical lac operators to ensure optimal repression prior to induction. These vectors are available with either the T7 or T5 promoter. DNA2.0's research has shown ...

Omaxe Infrastructure & Construction Private Ltd Receives LoI for Construction of Allopathic Medical College and Associated Hospital at Saharanpur, UP With Construction Value of Approx 250 Crores

...RNNL. The construction value of the project is Rs250 crores approx. The project is spread over an area of 62 acres with total built up area of 15.66 lac sq ft. It will comprise of an Allopathic Medical College and a 500-bedded hospital. OICPL will also construct an Academic building, an auditorium, a...

Wisconsin Development Fund awards announced

...vestment. Companies that received Wisconsin Technology Development grants eMetagen - Madison - $150,000 Heritage Wafer, LLC - Fond du lac - $22,560 Marchant-Schmidt, Inc. - Fond du Lac - $32,000 Perkins Engineering Co., Inc. - Sussex - $12,100 R. J. Zeman Tool & Mfg. Co., Inc....

Charter Business powers distance learning

...or Distance Education) network were installed and maintained by Charter Business. Charter Business also linked the MATC campuses of West Bend, Fond du lac and Beaver Dam in a distance education project. KSCADE, a Fox Valley-based network, is currently the largest DEN in the country (based on number of si...

Wisconsin entrepreneurs need to self-promote like coastal rivals

...cture was finished, a man in the audience raised his hand. He wanted to know whether the Fox Valley the part of Wisconsin that stretches from Fond du lac to Green Bay had enough entrepreneurial activity to support a network of angel investors. When I heard this, I realized that there is a huge diffe...

Vector Surgical hopes to turn frustration into faster surgeries

...where she led beta testing operations with surgeons and other physicians. Vector will be working with Converting Biophile Laboratories in Fond du lac to manufacture the two products. CBL supplies medical device components to several well-known companies, including Medtronic, 3M and Boston Scientific...

State campaign seeks to reverse negative perception of manufacturing work

...said. It was later embraced as a statewide initiative. Declining Enrollment Spurs Action At Moraine Park, with campuses in Beaver Dam, Fond du lac and West Bend, manufacturing program enrollment slid by 34 percent from 2003 to 2004, according to Holmes. "This sent up red flags," she said. "Deans,...

UW-Madison engineers apply award-winning technology to road building

... Madison, Wis. Motorists driving along the DeNeveu Creek bridge on Highway 151 near Fond du lac will never notice the difference as they drive over a bridge that uses a new method of construction developed at UW-Madison. However, highway offic...

Verizon expands broadband service in Wisconsin

... U.S. Highway 12; Interstate 90/94 from Madison to Wisconsin Dells and from Madison to Janesville and Beloit; and U.S. Highways 41 and 45 from Fond du lac to Madison. Verizon said its new service will provide customers with upgraded broadband access on their laptops, e-mail on their PDAs , and V CAS...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

...nes contain features designed for inducible high-level protein expression in bacterial cells. The vector contains the hybrid T7/lac O promoter and lac repressor gene (lac I) to regulate protein expression. Therefore, expression is inducible using isopropyl-1-thio-b-D-galatopyranoside (IPTG) in ...

Mammalian and Bacterial Expression in One Vector

...alian cells. Expression of heterologous genes in bacteria is regulated by a hybrid T7/ lacO promoter. The pDual vector was engineered to carry the lac repressor gene (lacI). Expression is therefore inducible using IPTG in bacteria that contain the T7 RNA polymerase. ## In both bacterial and m...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

...ctors released by Stratagene. 1 It is derived from the pET-11 vector series and contains the lacI q gene for expression of the lac repressor protein and the hybrid T7 promoter for controlled expression of the inserted protein coding sequence. 7,8 The vector ...

XL10-Gold Cells Supplied in New Single-Reaction Format

...taining high efficiency. Genotype XL10-Gold strain: Tet r D (mcrA)183 D (mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F proAB lacI q Z D M15 Tn10 (Tet r ) Amy Cam r ] The XL10-Gold strain is tetracycline and chloramphenicol resistant. REFERENCES ...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

...vectors released by Stratagene. It is derived from the pET-11 vector series and contains the lacI q gene for expression of the lac repressor protein and the hybrid T7 promoter for controlled expression of the inserted protein coding sequence. 8 The vector ...

Highest Possible Transformation Efficiencies for High-Throughput Applications

... Genotype XL10-Gold strain: Tet r D(mcrA)183 D(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F proAB lacI q ZDM15 Tn10 (Tet r ) Amy Cam r ] * U.S. Patent Nos. 5,512,468 and 5,707,841 and patents ...
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