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LIC in Biological Technology

New Competent Cells for Highest Transformation Efficiencies

...fficiency of transforming XL10-Gold cells with a ligated DNA molecule. The pCAL-n-EK vector (8.0 kb) from Stratagenes Affinity lic cloning kit was digested and prepared for the ligation-independent cloning (LIC) reaction using standard conditions. 2 The kana...

Randomize Gene Sequences with New PCR Mutagenesis Kit

... PCR primers were designed to introduce lic (ligation independent cloning) sites for cloning into the Affinity lic vector. Mutazyme produced high yields of ...struct. The PCR primers were designed to introduce lic (ligation independent cloning) sequence...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

... cDNA synthesized from small amounts of RNA with lic for high-efficiency, directional cloning. Th... by PCR using primers with specifically designed lic ends. 1-3 The PCR products are ready for purifi...irectional cloning into a vector with compatible lic overhangs. The kit offers numerous advantages over...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

...ired protein sequence into the pCAL-n-EK vector. lic creates seamless cloning junctions between the p...equire ligation or restriction enzyme digestion. lic creates a seamless junction 4,5,6 between the EK ... flexibility, Stratagene has refined the lic method for cloning inserts into the pCA...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

...tagene Stratagene has improved the Affinity lic cloning kit with the new pCAL-n-FLAG vector. Thi... protein coding sequence of interest, cloning by lic is highly efficient. Cloning efficiencies of 2... (MCS), and the T7 transcriptional terminator. lic Cloning of JNK ...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

...er Stratagene Cloning Systems, Inc. The ESP lic cloning and expression system features rapid and h... figure 1 Stratagenes ESP lic cloning and expression system offers rapid ...tor offers is efficient and directional lic 2-4 cloning of PCR products immediately ...

A New C-Terminal GST Vector for Protein Production in S. pombe

...roducts through ligation-independent cloning (LIC). 12 When the lic method is used, polypeptides without extraneously added amino ac...available separately and as components of the ESP system and ESP lic systems, respectively. The ESP system has been extensively teste...
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Breaking Biology News(10 mins):Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 2Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 3Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 5
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Breaking Medicine News(10 mins):Health News:Novel Biostatic Antimicrobial Technology From Clearstream-mPact Technology Partnership (CmTP) Offers Effective Protection Against Transmission of Ebola Virus 2Health News:Novel Biostatic Antimicrobial Technology From Clearstream-mPact Technology Partnership (CmTP) Offers Effective Protection Against Transmission of Ebola Virus 3Health News:Novel Biostatic Antimicrobial Technology From Clearstream-mPact Technology Partnership (CmTP) Offers Effective Protection Against Transmission of Ebola Virus 4Health News:Novel Biostatic Antimicrobial Technology From Clearstream-mPact Technology Partnership (CmTP) Offers Effective Protection Against Transmission of Ebola Virus 5Health News:Bob Woodruff Foundation Poised to Reach More Vets Thanks to Banner Year 2Health News:Bob Woodruff Foundation Poised to Reach More Vets Thanks to Banner Year 3Health News:Bob Woodruff Foundation Poised to Reach More Vets Thanks to Banner Year 4Health News:Bob Woodruff Foundation Poised to Reach More Vets Thanks to Banner Year 5Health News:Bob Woodruff Foundation Poised to Reach More Vets Thanks to Banner Year 6Health News:Bob Woodruff Foundation Poised to Reach More Vets Thanks to Banner Year 7Health News:You Still Have Time to Give the Gift of Health and Vitality to a Mature Loved One 2Health News:Panel Orders Xarelto Lawsuits Consolidated Into Multi-District Litigation Reports Alonso Krangle LLP 2Health News:Panel Orders Xarelto Lawsuits Consolidated Into Multi-District Litigation Reports Alonso Krangle LLP 3Health News:Panel Orders Xarelto Lawsuits Consolidated Into Multi-District Litigation Reports Alonso Krangle LLP 4
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