Navigation Links
LIC in Biological Technology

New Competent Cells for Highest Transformation Efficiencies

...fficiency of transforming XL10-Gold cells with a ligated DNA molecule. The pCAL-n-EK vector (8.0 kb) from Stratagenes Affinity lic cloning kit was digested and prepared for the ligation-independent cloning (LIC) reaction using standard conditions. 2 The kana...

Randomize Gene Sequences with New PCR Mutagenesis Kit

... PCR primers were designed to introduce lic (ligation independent cloning) sites for cloning into the Affinity lic vector. Mutazyme produced high yields of ...struct. The PCR primers were designed to introduce lic (ligation independent cloning) sequence...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

... cDNA synthesized from small amounts of RNA with lic for high-efficiency, directional cloning. Th... by PCR using primers with specifically designed lic ends. 1-3 The PCR products are ready for purifi...irectional cloning into a vector with compatible lic overhangs. The kit offers numerous advantages over...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

...ired protein sequence into the pCAL-n-EK vector. lic creates seamless cloning junctions between the p...equire ligation or restriction enzyme digestion. lic creates a seamless junction 4,5,6 between the EK ... flexibility, Stratagene has refined the lic method for cloning inserts into the pCA...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

...tagene Stratagene has improved the Affinity lic cloning kit with the new pCAL-n-FLAG vector. Thi... protein coding sequence of interest, cloning by lic is highly efficient. Cloning efficiencies of 2... (MCS), and the T7 transcriptional terminator. lic Cloning of JNK ...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

...er Stratagene Cloning Systems, Inc. The ESP lic cloning and expression system features rapid and h... figure 1 Stratagenes ESP lic cloning and expression system offers rapid ...tor offers is efficient and directional lic 2-4 cloning of PCR products immediately ...

A New C-Terminal GST Vector for Protein Production in S. pombe

...roducts through ligation-independent cloning (LIC). 12 When the lic method is used, polypeptides without extraneously added amino ac...available separately and as components of the ESP system and ESP lic systems, respectively. The ESP system has been extensively teste...
Other Tags
(Date:8/22/2014)... strong antioxidants and can reduce free radical ... against oxidative stress? Jianbo Zhao and co-workers ... Medical University, China discovered that green tea ... neuronal apoptosis, indicating green tea polyphenols play ... under oxidative stress. The relevant study has ...
(Date:8/21/2014)... Athens, Ga. An international team of scientists including researchers ... Brassica napus commonly known as canolain the journal ... of the plant, which is used widely in farming and ... and its native Europe, but the winter crop is increasingly ... for its naturally low levels of saturated fat and rich ...
(Date:8/21/2014)... unknown process for harvesting energy and producing oxygen from ... University scientist has discovered. The discovery lays the foundation ... energy from the Sun, and understanding dense blooms like ... worldwide. A paper describing the discovery will be published ... journal Science on 21 August 2014. , ...
Breaking Biology News(10 mins):Canola genome sequence reveals evolutionary 'love triangle' 2Hot-spring bacteria reveal ability to use far-red light for photosynthesis 2Hot-spring bacteria reveal ability to use far-red light for photosynthesis 3
(Date:8/22/2014)... Dayton Children's Hospital broke ground on a ... a $141 million campus renewal. Renderings of ... to the public. , The new patient tower is ... a campus that meets the needs of patients, families and ... will embody Dayton Children’s unique patient care mission and offer ...
(Date:8/22/2014)... again attained the distinguished Magnet hospital designation by ... Program. Considered the gold standard for nursing excellence, ... and professionalism. It is the top international credential ... redesignate Newport Hospital was based on the hospital,s ... practice. , "It is a privilege for us ...
(Date:8/22/2014)... who use reduced-nicotine cigarettes don,t smoke more to make ... a new study. This means they don,t inhale ... The month-long study included 72 adult smokers, aged ... emission levels of 1.2 milligrams (mg) each for one ... the next three weeks. In each of those ...
(Date:8/22/2014)... 2014 (HealthDay News) -- Inner city children have a higher-than-normal ... Researchers followed 516 inner city children in four U.S. cities ... birth until age 5 and found that at least 10 ... peanuts. Because only the three most common types of ... of inner city youngsters with food allergies may be even ...
(Date:8/22/2014)... A new, online conference is coming August 25th-29th, ... no-cost and open to everyone. Over 20 top experts ... advice on how to stop emotional eating, start eating healthier ... eating is not a diet. The concept is about how ... "eat this, don't eat that" approach. , You can sign ...
Breaking Medicine News(10 mins):Health News:Dayton Children's Hospital Breaks Ground on New, Eight-story Patient Care Tower 2Health News:Dayton Children's Hospital Breaks Ground on New, Eight-story Patient Care Tower 3Health News:Dayton Children's Hospital Breaks Ground on New, Eight-story Patient Care Tower 4Health News:Dayton Children's Hospital Breaks Ground on New, Eight-story Patient Care Tower 5Health News:Newport Hospital receives nursing's highest credential for third time with Magnet recognition 2Health News:Low-Nicotine Cigarettes May Not Lead to More Smoking 2Health News:Food Allergies More Common Among Inner City Kids, Study Finds 2Health News:20 Top Experts Discuss How to Unravel the Food-Mood Connection 2
Other Contents