New Competent Cells for Highest Transformation Efficiencies
transforming XL10-Gold cells with a ligated DNA molecule. The pCAL-n-EK
vector (8.0 kb) from Stratagenes Affinity lic
kit was digested and prepared for the ligation-independent cloning (LIC)
reaction using standard conditions. 2 The kana...
Randomize Gene Sequences with New PCR Mutagenesis Kit
... PCR primers
were designed to introduce lic
(ligation independent cloning) sites for
cloning into the Affinity lic
vector. Mutazyme produced high yields of
...struct. The PCR primers were designed to introduce lic
independent cloning) sequence...
Constructing Directional cDNA Libraries from Limited Amounts of RNA
synthesized from small amounts of RNA with lic
for high-efficiency, directional
Th... by PCR
using primers with specifically designed lic
ends. 1-3 The PCR
products are ready for purifi...irectional cloning into a vector with
overhangs. The kit offers numerous advantages over...
Expression and Purification of Recombinant Proteins That Have Native Amino
sequence into the pCAL-n-EK vector. lic
creates seamless cloning junctions
between the p...equire ligation or restriction
enzyme digestion. lic
creates a seamless junction 4,5,6 between the EK
flexibility, Stratagene has refined the lic
method for cloning inserts
into the pCA...
High-Level Protein Expression, One-Column Purification, and FLAG
Epitope Tagging in E. coli
Stratagene has improved the Affinity lic
with the new pCAL-n-FLAG vector. Thi...
protein coding sequence of interest, cloning by lic
is highly efficient. Cloning
efficiencies of 2... (MCS), and the T7 transcriptional terminator.
Cloning of JNK
New Yeast Cloning System for Producing Proteins with Native Amino Acid
Stratagene Cloning Systems, Inc.
The ESP lic
cloning and expression system features rapid and h... figure
Stratagenes ESP lic
cloning and expression system offers rapid
...tor offers is efficient
and directional lic
2-4 cloning of PCR products immediately
A New C-Terminal GST Vector for Protein Production in S. pombe
through ligation-independent cloning (LIC). 12 When the lic
method is used, polypeptides without extraneously added amino ac...available separately and as components
of the ESP system and ESP lic
systems, respectively. The ESP system has
been extensively teste...