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LCM in Biological Technology

RNA from LCM Samples

Juanita Gonzales, Ambion, Inc. Obtaining specific populations of cells from heterogenous samples is a major challenge in studying gene expression profiles of specific cell types. Laser capture ...

Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis

RNAqueous-Micro Kit Ideal for Processing Laser Capture Microdissection Samples Further development of Ambion's RNAqueous-Micro Kit has resulted in a 3-6 fold increase in total RNA yields from LCM samples. In addi...

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

...dies in a heterogeneous tissue. Specificity of the lcm of the neural crest cells was confirmed by quantit...m molecular analyses. In traditional applications, lcm is used to isolate cells that are distinguished by...fic EGFP expression with the powerful technique of lcm allows the isolation of RNA from cell types, which...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

...9674; Cupric Sulfate (Sigma, Cat. # C-1297) ◊ CapSure HS lcm Caps (Arcturus, Cat. # LCM0214) ◊ FastStart Taq DNA Poymer...histochemistry and innervation pattern allows us time to process tissue via lcm and isolate mRNA without significant time for degradation. Using ...

A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays

...te: Do not allow slides to come to room temperature after mounting section(s). k. Slides were immediately stained with the HistoGene lcm Frozen Staining Kit. Those not stained immediately were stored at 70C until use. 2.2 Paraffin Sections: a....

Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

... ISOLATION OF SPECIFIC CELL POPULATION BY lcm ANALYSIS lcm was performed using a PixCell II system (Arcturus, Cat# LCM1106) using CapSure Macro lcm caps (Arcturus, Cat# LCM0211). Pure luminal epithe...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

...cers as markers for microdissecting cell bodies by lcm (Lombardino et al., 2005). The RNA purified from t...ce (Arcturus, Cat. #LCM1104) ◊ Capsure HS lcm caps (Arcturus, Cat. #LCM0214) IV. RNA PU...tOH at least twice each day, and more often if the lcm pick-up is not optimal. 2. Dehydration pr...

Microgenomic Expression Profiling

...aser Microdissection System, Arcturus Bioscience). lcm utilizes an infrared laser pulse system which adhe... before proceeding, to evaluate tissue processing, lcm and RNA extraction procedures, and repeat steps as...ession profiling from extremely limited cell mass. lcm and LC provide the means of precise cell separatio...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...ue. This application note describes a protocol for lcm of cells identified immunohistochemically, followe...bstacles, we describe here a method for the use of lcm (7, 8) to selectively procure macrophage foam cell...urus, Cat. #LCM1104) ◊ CapSure Macro lcm caps (Arcturus, Cat. #LCM0211) IV. RNA EXTRA...

Working With Laser Capture Microdissection Samples

...f cells from heterogeneous samples. lcm permits the selection and capture of ... Formalin vs. Frozen Sections lcm is usually carried out on frozen se...detailed analysis of RNA recovered from lcm sections after various steps of the conventional ...

Protect RNA and Improve Target Cell Identification

... lcm Staining Kit ... Compatible with Arcturus lcm Systems Use with ...sis lcm for RNA Analyses Laser capt...

Is RNA Amplification Necessary for Microarrays?

...s experiments. Amplification of RNA is clearly necessary when working with limited samples (needle biopsies, lcm samples, etc.), but is it necessary when the availability of sample is not limiting? Is there additional bio...

Optimum FFPE RNA Isolation Kit

... Filter Cartridge allows RNA to be eluted in a concentrated form. The kit is also compatible with cells from lcm samples. Why use FFPE tissues? FFPE tissues are the most widely available specimens for retrospectiv...

Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation

...eadily analyzed. The kit can be used with transfected or treated cultured cells, and is also compatible with lcm and FACS sorted samples. The Cells-to-cDNA II Kit contains all the necessary reagents for 40 or 100 reverse ...

Cells-to-cDNA II Applications

... optimized RNA interference protocol. In addition to analyzing siRNA data, Cells-to-cDNA can be used to process FACS and lcm samples. The kit has also been adapted to 96 well plate automated processing. See
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