RNA from LCM Samples
Juanita Gonzales, Ambion, Inc.
Obtaining specific populations of cells from
heterogenous samples is a major challenge in studying gene
expression profiles of specific cell types. Laser capture
Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
RNAqueous-Micro Kit Ideal for Processing
Laser Capture Microdissection Samples
Further development of Ambion's
RNAqueous-Micro Kit has resulted in a 3-6 fold increase in
total RNA yields from LCM
samples. In addi...
Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
...dies in a heterogeneous tissue. Specificity of the lcm
of the neural crest cells was confirmed by quantit...m molecular analyses. In traditional applications, lcm
is used to isolate cells that are distinguished by...fic EGFP expression with the powerful technique of lcm
allows the isolation of RNA from cell types, which...
Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
...9674; Cupric Sulfate (Sigma, Cat. # C-1297)
◊ CapSure HS lcm
Caps (Arcturus, Cat. # LCM0214)
◊ FastStart Taq DNA Poymer...histochemistry and innervation pattern allows us time to process tissue via lcm
and isolate mRNA without significant time for degradation.
A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
...te: Do not allow slides to come to room
temperature after mounting section(s).
k. Slides were immediately stained
with the HistoGene lcm
Staining Kit. Those not stained
immediately were stored at 70C
2.2 Paraffin Sections:
Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
ISOLATION OF SPECIFIC CELL POPULATION BY lcm
was performed using a PixCell II system (Arcturus, Cat# LCM1106) using CapSure Macro lcm
caps (Arcturus, Cat# LCM0211). Pure luminal epithe...
Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
...cers as markers for microdissecting cell bodies by lcm
(Lombardino et al., 2005). The RNA purified from t...ce (Arcturus, Cat. #LCM1104)
◊ Capsure HS lcm
caps (Arcturus, Cat. #LCM0214)
IV. RNA PU...tOH at least twice each day, and more often if the lcm
pick-up is not optimal.
2. Dehydration pr...
Microgenomic Expression Profiling
...aser Microdissection System, Arcturus Bioscience). lcm
utilizes an infrared laser pulse system which adhe... before proceeding, to evaluate tissue processing, lcm
and RNA extraction procedures, and repeat steps as...ession profiling from extremely limited cell mass. lcm
and LC provide the means of precise cell separatio...
Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions
...ue. This application note describes a protocol for lcm
of cells identified immunohistochemically, followe...bstacles, we describe here a method for the use of lcm
(7, 8) to selectively procure macrophage foam cell...urus, Cat. #LCM1104)
◊ CapSure Macro lcm
caps (Arcturus, Cat. #LCM0211)
IV. RNA EXTRA...
Working With Laser Capture Microdissection Samples
...f cells from
heterogeneous samples. lcm
permits the selection and capture of
... Formalin vs. Frozen
is usually carried
out on frozen se...detailed analysis of
RNA recovered from lcm
sections after various steps of the conventional
Protect RNA and Improve Target Cell Identification
Compatible with Arcturus lcm
for RNA Analyses
Is RNA Amplification Necessary for Microarrays?
Amplification of RNA is clearly necessary when working with
limited samples (needle biopsies, lcm
samples, etc.), but is
it necessary when the availability of sample is not limiting?
Is there additional bio...
Optimum FFPE RNA Isolation Kit
Cartridge allows RNA to be eluted in a concentrated form. The
kit is also compatible with cells from lcm
Why use FFPE tissues? FFPE tissues are the most widely
available specimens for retrospectiv...
Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
...eadily analyzed. The kit can be
used with transfected or treated cultured cells, and is also
compatible with lcm
and FACS sorted samples. The Cells-to-cDNA
II Kit contains all the necessary reagents for 40 or 100
Cells-to-cDNA II Applications
... optimized RNA interference protocol. In
addition to analyzing siRNA data, Cells-to-cDNA can be used to
process FACS and lcm
samples. The kit has also been adapted to 96
well plate automated processing. See
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