Isolation

Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood

...

High-Throughput Isolation of Total RNA

Stratagene 96 total RNA purification kitprovides a convenient method for simultaneous isolation of total RNA from up to96 cultured cell samples. Because RNA is bound to a solid support, there is noneed fo...

Simple Isolation of RNA from Tissue and Cultured Cells

Stratagene total RNA miniprep kit efficientlyisolates high-quality total RNA from 10 culturedcells or 10 to 40 mg of tissue and includes an on-column DNA-removal step.Additionally, multiple sa...

Fast Isolation of Total RNA from Small Numbers of Cells

Extract total RNA from as few as 1 cell Stratagene total RNA microprep kit provides aconvenient method for isolating total RNA from small samples of cells. Forthe purification, RNA is bound to a solid support, which eliminates theneed for organic extraction and alcohol precipita...

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf<sup > Plasmid Mini Kit BioSystems Laboratory, Brinkmann Instruments, Inc., We...

Isolation of Microorganisms

Jrgen Frhlich and Helmut Knig; Institute for Microbiology and Wine Research, Johannes-Gutenberg-University; Mainz, Germany Dietmar Kahle, Eppendorf AG The isolati...

Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. It is not convenient in all instances to have blood drawn for gDNA extraction. The Eppendorf Perfect gDNA Blood Mini Kit was used to extract gDNA from...

Mouse Tail Genomic DNA Isolation Protocol(1)

Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA. Place a 1 cm tail sample into a 1.5 ml microcentrifuge tube; this may be stored...

Genomic DNA Isolation Protocol(1,2,3)

Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA. Caution: Wear suitable...

Basic Plasmid DNA Isolation Protocol

PLG can be used in small and large scale protocols based on alkaline lysis procedure.(1,2,3) The basic protocol given here works with E. coli cultures grown for 1214 hours in up to 500 ml of amplified or non-amplified LB or DYT, or in 250 ml Terrific Broth....

Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

Phase Lock Gel may be used in conjunction with TRIzol Reagent for the isolation of total RNA from cell and tissue samples. Increased yields are observed using this method, because the entire aqueous phase can be recovered without interphase contamination. Below is a protocol outlining the steps involved in RNA...

RNA-free Plasmid DNA Isolation Protocol

The following protocol is for use with 1 liter cultures grown in LB medium for 1214 hours. It is also used for cultures grown for no longer than 1214 hours in 0.5 liter of enriched growth media such as Terrific Broth (TB). The reagents used in the cleared lysate preparation (step 15) should be scaled...

Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. It is not convenient in all instances to have blood drawn for gDNA extraction. The Eppendorf Perfect gDNA Blood Mini Kit was used to extract gDNA from...

Basic Plasmid DNA Isolation Protocol

PLG can be used in small and large scale protocols based on alkaline lysis procedure.(1,2,3) The basic protocol given here works with E. coli cultures grown for 1214 hours in up to 500 ml of amplified or non-amplified LB or DYT, or in 250 ml Terrific Broth....

Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

Phase Lock Gel may be used in conjunction with TRIzol Reagent for the isolation of total RNA from cell and tissue samples. Increased yields are observed using this method, because the entire aqueous phase can be recovered without interphase contamination. Below is a protocol outlining the steps involved in RNA...

Genomic DNA Isolation Protocol(1,2,3)

Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA. Caution: Wear suitable...

Mouse Tail Genomic DNA Isolation Protocol(1)

Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA. Place a 1 cm tail sample into a 1.5 ml microcentrifuge tube; this may be stored...

RNA-free Plasmid DNA Isolation Protocol

The following protocol is for use with 1 liter cultures grown in LB medium for 1214 hours. It is also used for cultures grown for no longer than 1214 hours in 0.5 liter of enriched growth media such as Terrific Broth (TB). The reagents used in the cleared lysate preparation (step 15) should be scaled...

Total RNA Isolation Protocol 1,2,3

To extract RNA from washed and pelleted cultured cells, add 200 l 4 M Guanidinium Isothiocyanate Solution (4M Guanidinium Isothiocyanate, 25 mM Sodium Citrate, pH 7.0, 1 M b-Mercaptoethanol) to 0.5 x 10 cel...

Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit

Plasmid Mini Kit James Griffith, University of Georgia, Department of Genetics, Athens, GA Kimberly Vollmer, Brinkmann Instruments, Biosytems Application Lab, Westbury, NY...

Eppendorf Perfectprep Plasmid DNA Isolation Kits

Plasmid DNA Isolation Kits Eppendorf Perfectprep Plasmid DNA Isolation Kits are designed to generate high yields of highly pure plasmid DNA suitable for automated fluorescent sequencing, transfection, PCR, and other extremely sensitive downstream applications....

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Sonja Vorwerk, Max Planck Institut fr Zchtungsforschung, Carl-von-Linn Weg 10, 50829 Cologne, Germany Phone: ++49-221-5062-441, Fax. ++49-221-5062-413, e-mai...

RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater

is a tissue storage solution that stabilizes and protects cellular RNA in fresh tissues. (Ambion's new RNA -ICE is designed specifically for use with frozen tissues - see "<A href="http://www.ambion.com/techlib/tn/103/1.html">Stop Grinding...

RNAqueous-96 RNA Isolation Kit Automation Protocol

This protocol was developed for the MultiPROBE II HT Liquid Handling System with a Gripper Integration Platform. Time required: 1 hr 20 min. For more information on the protocol, see the <A href="http://www.ambion.com/techlib/prot/fm_1920.pdf">RNAqueous-96 Instruction Manual...

RNAPrep - Total RNA Isolation Purification

based magnetic bead purification kit for extracting and purifying total RNA from Eukaryotic cultured cells. RNAPrep is a simple and reproducible 3-step procedure for isolating total RNA for use in applications such as microarray and RT-PCR gene expression analysis. RNAPreps automation friendly...

Isopure - Buccal cell DNA isolation

The Isopure Buccal Cell DNA Kit is a simple, streamlined method for isolating cheek buccal cell genomic DNA for downstream applications such as PCR. The kit supports swab or Scope mouthwash collection formats using SPRI magnetic bead technology that eliminates the need for filtration or centrifugation. The Isopure...

Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A

Joseph W. Turek and Guy C. Le Breton, Department of Pharmacology,University of Illinois at Chicago, College of Medicine, Chicago, IL 60612.Address correspondence to: Joseph W. Turek, 835 South Wolcott, Departmentof Pharmacology M/C 868, University of Illinois at Chicago, Chicago, IL 60612.E-mail: jturek1@icarus.uic.edu Introduction...

Automating RNA Isolation

Large scale gene expression screening projects require reliable high-throughput methods to isolate high quality RNA from a large number of samples. To meet this growing need, Ambion developed the RNAqueous-96 Kit . This kit, which utilizes an RNA-binding glass-fibe...

Bacterial mRNA Isolation, Fast and Easy

(patent pending) is currently the only commercially available bacterial mRNA purification kit. It uses a novel strategy that rapidly removes >95% of the 16S and 23S rRNA from total RNA of and other bacterial sp...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

90% of mammalian RNA from complex mixtures of host-bacterial samples Seamless integration with MICROB <...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

90% of mammalian RNA from complex mixtures of host-bacterial samples Seamless integration with MICROB <...

Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation

Quantitative or real time RT-PCR is one of the most commonly used techniques for quantitating changes in gene expression across samples. When working with cell or tissue samples for subsequent use in RT-PCR you must extract...

Custom RNA & RNA Isolation Services to Meet Your Specific Needs

Experienced staff designated for custom orders Flexible, optimized protocols to meet your specific needs Established network of ethical Tissue Procurement Partners to obtain rare and unique tissue samples...

Improve RNA Isolation for Array Analysis and More

Researchers performing array analysis have found that a combination of phenol based and solid phase RNA extraction methods often provide superior array data than either method alone (1-3). Ambion's new line...

Efficient Cellular Fractionation for RNA and Protein Isolation

Efficient Cellular Fractionation for RNA Ambion's Protein and RNA Isolation System, PARIS, uses a rapid and simple procedure to isolate both total RNA and native protein from the same experimental sample. Cultured cells and tis...

Optimum FFPE RNA Isolation Kit

Phenol-free isolation of total RNA from FFPE tissues <A href="http://www.ambiondx.com/products/optimum/index.html">Optimum FFPE RNA Isolati...

High Quality RNA Isolation Services

Use Ambion's Custom Isolation Services...

Isolation to Functional Analyses

While the microRNA (miRNA) field is rapidly expanding, these small, non-poly(A) tailed molecules still pose challenges for research. Most RNA analysis techniques have been optimized for longer molecules, not small 21-23 nt RNAs. Their small size precludes standard RT-PCR analy...

High Throughput RNA Isolation for High Performance Microarray Analysis

Protocol combines benefits of TRI Reagent sample lysis with MagMAX magnetic bead-based RNA isolation technology Procedure is compatible with fresh, flash-frozen, or RNAlater-treated tissues...

RNAlater FAQs: Preserving RNA Before Isolation

Appropriate storage of cells or tissue samples prior to RNA isolation is critical for preservation of intact RNA within those samples. RNA is an aqueous solution designed specifically to maintain RNA integrity during storage of fresh tissue and cell samples. Here are some...