Inc

Principles and applications of electrical cell fusion

Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cance...

Principles and applications of electrical cell fusion

Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cance...

cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR

RT-PCR has become an essential method that allows researchers to determine the presence, structure and level of expression of an mRNA transcript in the organism, tissue or cell of interest. The basic protocol involves the synthesis of a cDNA copy of all the mRNA present in the s...

Grow Milwaukee includes millions for tech funding

The package, which Doyle alluded to in last week's <a href="http...

In an increasingly 'flat' world, Wisconsin must adapt to compete

Speaking to business leaders in Milwaukee, Hewlett-Packard vice president Kevin Gilroy said the "hypercompetitive global economy" has made it difficult for companies to stand out in the crowd. The worldwide "digital transformation" means facto...

Want a raise? Ten best midwest tech salary-increase pitches

You know you have to have a great argument for your boss to get him on board with your pay-raise proposal. You also know that dollars are tight and your boss is pre-disposed (or programmed after several years of heart-rending pleas fro...

Rockwell Q2 operations net income soars, shares plunge

Income from continuing operations at Milwaukee-based Rockwell Automation Inc. for the quarter ended March 31 rose to $142.5 million, a 90 percent leap from the $74.9 million the company earned the same quarter of 2004. Profit for the second quarter of 2005, including discontinued operations, was an even $150 million, compared to $78.3 million a year ago. Despite that, Rockwell s...

National stem-cell panel, including UW profs, urges close watch on research

Research institutions should create oversight committees to guide human embryonic stem-cell studies according to common guidelines, a panel of experts, including two University of Wisconsin-Madison professors, said on Tuesday in Washington, D.C. "In the absence of federal guidelines broadly governing the generation and research use of human embryonic stem cells, the scientific community...

Governor Doyle Plans To Increase Web Access

MADISON, WI Governor Jim Doyle today announced his "Grow Wisconsin" initiative to drive state economic development. One of the recommendations is to increase broadband and communications access for all residents in the state. Mike Tye, AT&T regional vice present of Law and Government Affairs, commented that AT&T is encouraged by the "Grow Wisconsin" initiative unveiled today by Wisconsin...

Trouble-shooting: Misincorporation or low fidelity

Here are some troubleshooting hints that we have gathered regarding misincorporation or low fidelity of PCR reactions: <a href="#Option1">Magne...

PCR principles

In April, 1983, Kary Mullis took a drive on a moonlit California mountain road and changed the course of molecular biology. During that drive, he conceived the Polymerase Chain Reaction (PCR). As is shown in the figure, the reaction uses two oligonucleotide...

Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase

Transcriptor Reverse Transcriptase is a new recombinantreverse transcriptase (RT) expressed in E. coli. Theenzyme has RNA-directed DNA polymerase activity,DNA-dependent DNA polymerase activity, unwindingactivity, and, importantly, RNase H activity....

Increased Sensitivity in Microarray Analysis

Transcriptor Reverse Transcriptase inthe Eberwine Target Preparation Workflow Reproducibility and sensitivity are key parameters for thequality of a gene expression profiling experiment. Theimproved reliability of commercially avail...

Increase Protein Activity and Yield

Higher yields of protein than reticulocyte lysate systems lysate systems Easy to...

Selecting siRNA Sequences to Incorporate into the pSilencer Vectors

The first step in preparing a plasmid for siRNA experiments is to identify target sequences in the gene of interest that are susceptible to siRNA-induced degradation. We have found that a little more than half of the siRNAs provide at least a 50% reduction in target mRNA levels and approximately 1 out of 4 siRNAs...

Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B

Diana Davis, PhD, Aiguo Zhang, PhD, Chloe Etienne, Ivan Huang,and Michele Malit, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive,Hercules, CA 94547 USA Bio-Plex cytokine assays are bead-based multiplex sandwich immunoassays. The concentrations of analytes in a Bio-Plex cytokine immunoassay are...

Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell

Semi-dry transfer has traditionally been a fast but inefficient method for transferring all the protein from a gel. By using a discontinuous buffer system, transfer efficiency can be greatly increased. In protein blotting, the buffer has an important effect on the elution of the proteins from a gel...

Integration of the LEADseeker Multimodality Imaging System with automated plate loading and incubation using Overlord Instrument software

n The LEADseeker™ Multimodality Imaging System is a CCD-based optical imaging instrument that efficiently detects and quantitates light from a range of radiometric, luminescence, and fluorescence assays. Throughput of over 100 plates per hou...

Increase Your Organizations Productivity with LabVIEW

This paper addresses managers in different industries and describes the benefits of implementing their applications in LabVIEW. It describes how LabVIEW increases productivity, and touches upon subjects such as ease of use, complete functionality, available tools and resources, and performance. This paper also describes the main criteria managers should consider when choosing th...

A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)

A previously published application note in this series describes an LC/MS/MS method for the simultaneous determination of four immunosuppressant drugs: Cyclosporin A (CsA), Tacrolimus (TAC, aka FK-506), Sirolimus (SIR, aka Rapamycin) and Everolimus (EVE, aka RAD-001) (1, 2). The method is based on a simple and robust hardware configuration and...

Food science: increasing crop yields

Protein-containing plants are staples in the diets of all countries. The ability to produce enough crops is essential to the well-being of all citizens, for people have to have enough to eat before they can advance to higher endeavors. <P>Uncontrollable changes in weather such as the amount of rainfall, the occurrence of fro...

Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery

Department of Drug Metabolism and Pharmacokinetics, Sch...

Analysis of a Verapamil Microsomal Incubation using Metabolite ID and Mass FrontierTM

The characterization of a drugs metabolites plays a crucial role throughout its development. In the early discovery p...

A technique for increasing yields in bioreactors and disposable cell-culture systems

FibraCel disks are a new cell-growth matrix that dramatically increase yields in packed-bed reactors, andhave also proven highly effective when used in spinners, disposable bottles and bags. New BrunswickScientific offers two new devices that use FibraCel disks to increase productivity: the FibraStage , a simpleand disposable c...

140-Fold Increase of Protein Achieved in Pichia Fermentation

NBS microbiologists recently assisted researchers at the University of California in achieving a140-fold increase in protein production from a recombinant strain of Pichia pastoris yeast. Topyields by the researchers thus far had been 1.4 mg/L of a throm-bomodulin fragment whenculturing Pichia in shake flasks. By switching to a BioFlo 3000 </...

Visions, Part II: Preizler sees IT leading incremental health system change

: We hear a lot about interoperability of information technology systems between health f...

DHC 2007: Barriers to electronic data exchange include the law

Patient data exchange was addressed in two sessions at , including one that posed the question of whether the el...

Healthcare of business: Universal coverage plan includes new business taxes

Following the lead of California and Massachusetts, Illinois has called he...

Whitewater makes plans for business incubator

at UW-Whitewater are developing a business incubat...

Damn the incumbents, and full speed ahead

Speed is the common measurement that cuts across many products and services as the general metric for assessing whether or not a produ...

Closing the income gap begins with education

A report by the...

Symposium will include venture capital "coaching"

All will be components of the second annual Wisconsin Early Stage Symposium, which will be held on Monday and Tuesday, Oct. 30 and 31, at Monona Terrace Convention Center. The symposium is co-produced by the <...

The science is clear: Ethanol yields a net increase in energy

The final report by the Governors Consortium on Bio-Based Industry, issued Friday, will no doubt spark another round of anti-ethano...

Baldwin backs funding for Midwest biotech incubator

Funding for the Midwest Biolink Incubator was included at the request of , D-Madison, in an appropriations bill passed Wednesday in the House of Representa...

A (modern) Tale of Two Cities: Milwaukee and Madison inch together

MADISON First, a full disclosure: Ive been hawking the concept of the I-Q Corridor connecting Chicago, Milwaukee, Madison and beyond for a couple of years, so its no surprise I would think its swell that the mayors of Milwaukee and Madison are finally finding common ground. Then again, its still something of a shock that it may be actually happening. You dont have to be a stu...

Water disruption affects Milwaukee business incubator

Guy T. Mascari, whose office is loc...

Act 255 clean-up law makes incentives work for VC funds

Tax-credit legislation for venture and angel investors has been "cleaned up" to make it more closely follow the original intent. is an incentives package for investors in early-stage companies. Both venture and angel investors can apply for tax credits of 25 percent of their investments in pre-qualified Wisconsin companies. A...

Computerworld top 100 includes three Wisconsin CIOs

Three Wisconsin chief information officers made the Computerworld magazine's top-100 list this year. They were: Carlton Harper, CIO of , in Racine. Modine specializes in thermal management systems such as cooling for a new soccer stadium in London and a carbon-dioxide-cooled Jeep, and has been active in fuel-cell development.<b...

University Research Park to add 20 incubator suites

Twenty suites ranging from about 700 square feet to nearly double that size will be added on the east side of the Innov...

Chippewa Valley receives state grant for nanotech incubator

"Through this project, we are creating an environm...