Immun

Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling

Danny Q. Hoang Michael Browning Xinping Yang PeterPingerelli Stratagene Cloning Systems, Inc. Stratagene introduces affinity purified antibodies for NMDA receptorsubunits that are useful for studying expression of the NR2A, NR2B and NR2Csubunit proteins...

Enzyme Immunoassay for Studying Intracellular Levels of cAMP

Sensitive, nonradioactive cyclic-AMP detection kit and adenylate cyclase(AC) toxin Stratagene Cloning Systems, Inc. American Qualex Stratagenes EIA cAMP Kit is a highly sensitive and specificantibody-based assay kit for no...

UW scientist wins Pew grant to study little-known immune cells

Jenny Gumperz, an assistant professor of medical microbiology and immunology, will receive $60,000 per year for the next four years from the Pew Charitable Trusts, an independent nonprofit organization that annua...

Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A

and Aiguo Zhang, 1 Department of Molecular and Clinical Medicine, Division of Paediatrics, Linkping University, Sweden, and Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA...

Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B

Diana Davis, PhD, Aiguo Zhang, PhD, Chloe Etienne, Ivan Huang,and Michele Malit, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive,Hercules, CA 94547 USA Bio-Plex cytokine assays are bead-based multiplex sandwich immunoassays. The concentrations of analytes in a Bio-Plex cytokine immunoassay are...

Real-Time Immuno-PCR on the iCycler iQ System, Rev A

Kristina Lind and Mikael Kubista, TATAA Biocenter, Medicinaregatan 7A/B,405 30 Gteborg, Sweden A technique for antigen detection, called immuno-PCR, was developed by Sano et al. (1992). It combines the molecular recognition of antibodies with the high DNA amplification capability of PCR. The procedu...

Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C

Eric Larson, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA PVDF membrane was originally introduced to protein use as an ideal medium for the harsh chemical environment of N-terminal, or Edman degradation, sequencing. Even though PVDF is very hydrophobic and...

A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)

A previously published application note in this series describes an LC/MS/MS method for the simultaneous determination of four immunosuppressant drugs: Cyclosporin A (CsA), Tacrolimus (TAC, aka FK-506), Sirolimus (SIR, aka Rapamycin) and Everolimus (EVE, aka RAD-001) (1, 2). The method is based on a simple and robust hardware configuration and...

Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System

Immunoassays are valuable and widely used tools in the biological sciences. In drug discovery research, immunoassays are used to measure secreted cytokines in the tissue culture supernatants of treated cells. However, scientists often avoid immunoassays in high throughput screening (HTS) because they require cumbersome manipulations. Applied B...

Affinity Depletion Cartridges for Removal of Human Serum Albumin and Immunoglobulins from Human Serum

One of the major difficulties in analyzing the proteome of human serum is the dynamic range of the concentrations of the proteins present in the sample. Human serum albumin (HSA) constitutes 57-71% of total serum protein and gammaimmunoglobulin (IgG) ranges from 8-26%. Removal of these two proteins alone clears about 75% of t...

Coated Plate Immunoassay on the MicroBeta - eg. TSH - IRMA

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Immunoassay of Theophylline by fluorescence polarisation

Fluorescence immunoassay is applied to the detection of theophylline, a drug used for myocardial stimulation. Interference from other sample components is limited by use of polarization measurements, which discriminate between bound and unbound fluorophores. The detection system includes a T-format sampling module mounting two emissio...

Immune boosting beverage relying on Milwaukee firm's packaging technology

New York-based is launching Defense Vitami...