Counting Colonies or Plaques Directly from Images of Agar Plates
Michelle Cayouette Jane Moores Michael Steege AlanOrr Stratagene Cloning Systems, Inc. software version 3.1,researchers have the ability to automatically count bacterial colonies andplaques on agar plates. Researchers can use...
Optimized Imaging of Protein Gels Stained with Coomassie
Brilliant Blue Dye
Brilliant Blue Dye Stratagene Cloning Systems, Inc. Stratagene currently provides an optical bandpass filter with all EagleEye II still video systems that is...
Detect Released Oligosaccharides Using the Eagle Eye II
Still Video Imaging System
IIStill Video
Imaging System Department of Biology, Delaware State University, Dover, DE II still video system andthe Glyko FAC...
Imaging Chemiluminescent Western Blots
II still video system to directly acquire
images from chemiluminescent blots Stratagene II still video system is well suited for direct
imaging of chemiluminescent Western blots. The light-tight cabinet is ideal forthe longer exposure times necessary to...
Eagle Eye Optical Filter Optimized for Imaging Silver-Stained
Gels
Optical Filter Optimized for
Imaging Silver-StainedGels Stratagene II Still Video System that increases contrast...
Japanese institute installs Imago system
The device, the LEAP 3000 Metrology System, is made by
Imago Scientific Instruments of Madison. LEAP stands for local electrode atom probe. It is used...
Two Milwaukee finance IT firms agree to exchange check images
Fiserv is an IT service provider claiming 4,000 financial clients. E...
Standard Imaging acquires Canadian PIPSpro radiation treatment technologies
Middleton, Wis. s assets. Among the assets acquired from the British Colum...
Standard Imaging acquires Canadian PIPSpro radiation treatment technologies
Middleton, Wis. assets. Among the assets acquired from the British Colu...
Imaging and Analysis of DIGE Product Information Sheet, Rev B
Difference gel electrophoresis (DIGE) is a relatively straightforward application of differential labeling of protein samples using fluorescent dyes. The technique, originally published by Unlu et al. (1997), uses cyanine (Cy) dyes to differentially label proteins separated by 2-D gel electrophoresis. Following labeling...
The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
CXCR2 • GPCR • receptor binding assay • LEADseeker • SPA
Imaging Beads Chemo-attractant cytokines (chemokines) are low molecular weight, pro-inflammatory cytokines with potent leukocyte activation and/or chemotactic activity (1). Chemokines are classified into four families (CXC, CC, C, and CX...
Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
AMARA • kinase assay • LEADseeker • chemiluminescence It is predicted that at least 500 kinase and phosphatase enzymes exist, their activities having wide-ranging regulatory responses. Many kinases are part of a signaling cascade in disease pathways such as inflammation and cancer, so identifying modulators of kinase...
The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
Melanocortin • receptor binding assay • LEADseeker • SPA
Imaging Beads Pro-melanocortins are present in high levels in the pituitary and are processed into three major peptide hormone fragments: adrenocorticotrophin (ACTH), the melanocyte stimulating hormones, and β-endorphin. The various melanocyte stimulat...
The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
Melanocortin • receptor binding assay • LEADseeker • SPA
Imaging Beads Pro-melanocortins are present in high levels in the pituitary and are processed into three major peptide hormone fragments: adrenocorticotrophin (ACTH), the melanocyte stimulating hormones, and β-endorphin. The various melanocyte stimulat...
Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
ECL Plex • Ettan DIGE
Imager • fluorescent Western blotting • multiplex detection • dynamic range The Ettan™ DIGE
Imager is fully compatible with the ECL Plex™ system, reaching the same levels of sensitivity, linearity, and dynamic range as the Typhoon™ scanner. Similar results are also obt...
The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
CGRP • receptor binding assay •LEADseeker • SPA
Imaging Beads Calcitonin-gene related peptide (CGRP) is believed to have an important physiological role in the central nervous and cardiovascular systems. Indeed, using CGRP receptors isolated from coronary and basilar arteries, studies have i...
Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
SPA
Imaging Beads • LEADSeeker • cAMP • receptor binding assay SPA
Imaging Assays are homogeneous immunoassays based on the coupling of one assay component to an SPA
Imaging Bead in order to generate a signal. Polystyrene (PS) SPA
Imaging Beads coated with donkey anti-rabbit antibody enable the immobilization of...
Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
receptor • GPCR • receptor binding assay • LEADseeker • SPA
Imaging Beads Histamine is a biogenic amine that plays important pathophysiological roles in central and peripheral tissues. Generally released from stimulated mast cells, it contributes to several biological processes that characteri...
Performance of fluorescent dyes with LEADseeker Multimodality Imaging System
Flexible and scalable uHTS programs demand that organizations be able to select the most appropriate or cost-effective fluorescent reagents for the task. This application note describes experiments to assess...
Ettan DIGE Imager
The Ettan™ DIGE
Imager (Fig 1) is a scanning CCD camera designed for applications in the life sciences. In particular, it has been engineered to create high quality
images of 2-D DIGE gels. By combining high resolution with precise motion control, the Ettan DIGE
Imager produces accurate multichannel
images of your Cy™2-, Cy3-, and Cy5-labeled gel. The system...
Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System
Competitive pressure is forcing organizations to continually increase screening throughput. Yet accuracy and sensitivity remain vital concerns, particularly for common screens such as luminescence assays. LEADseeker™ Multimodality
Imaging System was de...
Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System
Competitive pressure is forcing organizations to continually increase screening throughput. Yet accuracy and sensitivity remain vital concerns, particularly for common screens such as luminescence cell-based ass...
Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
Neurokinins, also known as tackykinins, are a family of small neuropeptides. Members of this family include peptides, substance P (SP), neurokinin A (NKA), neurokinin B (NKB), and the recently discovered hemokini...
The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System
Somatostatin (SS) is a neurotransmitter/hormone with a wide range of biological actions (1–3) and a reduction in cortical SS levels has been reported in Alzheimer’s and Parkinson’s diseases. The SS analog...
The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System
In mammals, serotonin (5-hydroxytryptamine, 5-HT) is a regulator of neural transmission which induces its biological effect by binding to serotonin receptors. <P >The serotonin 5-HT rece...
Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System
The α +...
Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
In the mammalian brain, the corticotrophin-releasing factor (CRF) system comprises four CRF-related peptides (CRF and urocortin 1, 2, and 3) and two G protein-coupled CRF receptor subtypes (CRF...
Integration of the LEADseeker Multimodality Imaging System with automated plate loading and incubation using Overlord Instrument software
n The LEADseeker™ Multimodality
Imaging System is a CCD-based optical
imaging instrument that efficiently detects and quantitates light from a range of radiometric, luminescence, and fluorescence assays. Throughput of over 100 plates per hou...
An Image-Based Assay of Endothelial Cell Tube Formation as a Model of Angiogenesis
Formation of new blood vessels is fundamental to the development of cancerous cell masses and has therefore been the focus of many drug screening and cell signaling research studies. Blood vessel development is a significant event in the development and growth of solid tumors, and is implicated in wound h...
Quantitative Analysis of the Cell Cycle in Imaging Applications
Quantitative analysis of stages in the cell cycle is commonly used in cell biology since many pharmacological interventions aim to modulate the cell cycle in some way. We present here an application that can be used in adherent cells and optionally multiplexed for the use in automated cell signaling analysis and drug-screen...
Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays
The understanding of the cellular signaling processes leading to programmed cell death (apoptosis) is of utmost importance in the study of autoimmune diseases such as rheumatoid arthritis or Parkinsons and Alzheimers disease. Here, errors in the signaling cascade are believed to lead to premature apoptosis in the affected t...
DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
Oxidative stress, radiation and other external insults have been shown to damage DNA molecules in cells derived from organisms as diverse as bacteria, yeast, drosophila, rodents and man (Friedberg et al. 2005). The presence of DNA damage may lead to cell cycle checkpoint arrest to allow time for DNA repair...
BD IMag Human Regulatory T Lymphocyte Separation Set for Isolation of Treg Cells
BD Biosciences is pleased to introduce the BD
IMag Human Regulatory T Lymphocyte Separation Set DM (Cat. No. 558142) for the isolation of Treg cells from peripheral blood mononuclear cells (PBMC). The reagent set isolates Treg cells in two stages. First, negative selection is performed using BD
IMag streptavidin particles with a cocktail of biotinylated monoclonal antibodies that rec...
A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology
Dept. of Biology, Wilfrid Laurier University, 75 University Ave. W., Waterloo, Ontario N2L 3C5, Canada...
Kodak in vivo imaging system: precise coregistration of molecular imaging with anatomical X-ray imaging in animals
Kodak Molecular
Imaging Systems introduces a line of small-animal in vivo
imaging instruments that provide a new level of molecular signal localization in live animals. The
Image Station In-Vivo FX allows precise multi-modal coregistration of optical or radioisotopic molecular
images with high-resolution anatomical X-ray
images in animals...
Near Infrared Technology and Optical Agents For Molecular Imaging
Molecular Biology Department LI-COR Biosciences Lincoln, NE 68504 Optical
imaging is a rapidly developing biomedical technology that enables the examination of cellularprocesses in the context of a living animal. While several optic...
Blurb shows that print still captures imaginations
There's no clearer example than . I first discussed Blurb with founder Eileen Gittens when the company was no more than a brief <a href="http://www.office.microsoft.com...
Madison's PunchStock acquired by Getty Images
Terms of the deal were not disclosed. The acquisition reportedly is part of a business strateg...
Merge unveils next-generation imaging-informatics product
Merge, a medical
imaging software and services company, will market the product, which is called Fusion RIS/PACS, to im...
Merge unveils updated medical imaging software
Merge, which has released several product upgrades this year as it attempts to recover from past financial reporting improprieties, said...