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A Versatile Power Supply for All Electrophoresis Applications

Douglas Drake Michelle Cayouette Lisa Filippone Bob LeSchofs Stratagene 4000 Power Supply Stratagene has developed the new SmartPower...

Highest Possible Transformation Efficiencies for High-Throughput Applications

Convenient, time-saving 96Pack Gold competent cells streamline transformation procedures Julie Bonnardel Ronda Allen Bruce Jerpseth Sean Banks...

prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

Michael Borns Janice Cline Kris Connors RondaAllen Holly Hogrefe Stratagene RT-PCR systems are powerfulsystems that permit efficient, high-fidelity amplification of RNA in twoconvenient formats. The ProSTAR Ultra HF RT-PCR...

Production of Full-Length, Biologically Active MEK1WT and MEK1CA

Stratagene Cloning Systems, Inc. Stratagene has successfully expressed and purified recombinant, wild-typehuman MEK1 )using the ESP yeast pro...

Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted

Quantitating cell number is more accurate using the Quantos cellproliferation assay kit Genetic Applications LLC cell proliferation assay kitprovides a simple, reproducible method to assess the number of cells present invarious tissue culture dish formats. In this st...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

Epitope Tagging in E. coli Katherine Felts Denise Wyborski John Bauer PeterVaillancourt Stratagene LIC cloning kit...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acidpurification kits Stratagene Cloning Systems, Inc. Stratagene announces a new line of DNA purification products, whichprovide reliable isolation of high-purity miniprep plasmid, PCR product andgel-fractionate...

Greater Amplification Specificity with New Hot Start PCR Enzyme

Taq DNA polymerase makes hot start simple and efficient Jonathan Eads Frances Bai Ronda Allen Holly Hogrefe <p align="...

Yeast Protein Production System Features High Yields and One-Step Purification

The ESP system provides high-yield protein production in a eukaryoticorganism Quinn Lu * Bruce Jerpseth * Tim Sanchez * John C. Bauer * AlanGreener Stratagene Cloning Systems, Inc. provides novel opportunities for rapid, inexpensive and high-yield p...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

Denise L. Wyborski * John C. Bauer * Barbara McGowan * JosephA. Sorge * Peter Vaillancourt Stratagene Cloning Systems, Inc. Stratagene has improved the Affinity protein expression andpurification system by the addition of the pCAL-n-EK vector. This E. coliclonin...

Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications

Herculase enhanced DNA polymerase delivers high fidelity and greatperformance Stratagene Stratagene has recently released Herculaseenhanced DNApolymerase,* a novel enzyme formulation that providessuperior performance in both routine and demanding PCR applications. Compariso...

Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase

DNAPolymerase Stratagene DNA polymerase* by making simple modifications to PCR reactionconditions. In this update, we d...

Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer

Optimizing pfuturbo DNA Polymerase Amplification Reactions withPerfect Match Stratagene Certain PCR amplification systems produce multiple pro...

Identification of Differentially Expressed Gene Products with the CastAway System*

CastAway precast gels for fast analysis of RAP-PCR and differentialdisplay Stratagene Cloning Systems, Inc. Stratagenes new CastAway precast sequencing gels were used to detectand isolate differentially expressed gene products amplified by the RNAarbitrarily primed polymerase chain reaction (R...

High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications

Simultaneous purification of 96 PCR products Stratagene Phenogenex 96 PCR purification kit is a newmember of the StrataPrep product line. This kit offers a high-throughput formatfor purifying ultra-pure PCR product DNA from la...

Easy Cosmid DNA Purification with StrataPrep Kits

Stratagene plasmid miniprep and midiprep kits aredesigned to isolate up to 20 and 350 g, respectively, of high-purity plasmidDNA from a single spin cup. These kits can also easily purify verylarge plasmids, inc...

Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels

Optical Filter Optimized for Imaging Silver-StainedGels Stratagene II Still Video System that increases contrast...

Improve Amplification Specificity with Hot Start PCR Enzyme

Taq2000 features a modified version of Taq2000 with hot start capability. Using SureStart Taq, hot start is in- corporated into PCR protocols previously optimized with Taq DNA...

Quantification made easy

With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories. It includes absorption measurements in the ultraviolet and in the visibility range. Proteins are measured (directly) at 280 nm, nucleic acids at 260 nm and colorimetric...

An Assessment of the Eppendorf twin.tec plate performance in PCR* applications

M. Arun Kumar, PhD, and Vincent Prezioso, PhD, Brinkmann BioSystems Laboratory To evaluate the performances of Eppendorf 96-well twin.tec PCR plates, we carried out PCR reactions with twin.tec plates and PCR microplates from another commercial mi...

A Unique Dimension in Biological Purity

Eppendorf AG With Eppendorf Biopur has created a unique standard of purity for consumables. Pipette tips, Safe-Lock/Flex Tube Microcentrifuge Tubes and Combitips bearing the Eppendorf...

Chemical Stability of Disposables

Disposables such as Eppendorf Tubes and pipette tips are frequently exposed to chemical reactions and they must withstand the various kinds of stress and strain encountered in everyday laboratory work (such as centrifuging and autoclaving). The stability level of these products is largely determined by the materials fr...

Principles and applications of electrical cell fusion

Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cance...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Optimizing Büchi® Rotary Evaporator Applications

Selecting the correct operating parameters for Büchi Rotary Evaporators is an important part of your application. Many variables must be considered, i.e. choosing the appropriate bath temperature, vacuum pressure, rate of evaporation, and acce...

Principles and applications of electrical cell fusion

Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cance...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Optimizing Büchi® Rotary Evaporator Applications

Selecting the correct operating parameters for Büchi Rotary Evaporators is an important part of your application. Many variables must be considered, i.e. choosing the appropriate bath temperature, vacuum pressure, rate of evaporation, and acce...

An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat

GBF Gesellschaft fr Biotechnologische Forschung mbH (Society for Biotechnological Research) DNA microarrays are a key technology in modern molecular biotechnology. Due to their high degree of parallelism, they allow the...

Chemical Stability of the Brinkmann Bottletop Dispenser and the Brinkmann Bottletop Buret

Bottletop Dispenser and the Brinkmann Bottletop Buret Bottletop dispensers are used in the lab for dispensing a wide range of different solvents from glass or stainless steel containers. These dispensers have to meet various requirements. For example, they must not give...

Optimizing Bchi Rotary Evaporator Applications

Rotary Evaporator Applications <TD rowSpan=2> <TD align=middl...

Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq

University of Georgia, Department of Marine Sciences This application describes the use of the MasterTaq polymerase kit for improving PCR efficiency when studying functio...

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

BIOGEM, Division of Biology, University of California San Diego, La Jolla, CA 92093-0349 e-mail: ghardiman@ucsd.edu www.biogem.ucsd.edu Microarray technology, primarily advanced by Pat...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In man...

Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler

Peter Scheinert, PhD, Barbara Behrens, PhD, and Dietmar Kahle, PhD Peter Scheinert, PhD, Bernhard-Nocht-Institute for Tropical Medicine, Virology Dept., Bernhard-Nocht-Str. 74, D-20359 Hamburg former address: University of Kiel, Institute for General Microbiology, Am Botanischen Garten, D-24118 K...

Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient

Using Eppendorf Mastercycler gradient NPC Laboratory, Institute of Postgraduate Studies and Research, University of Malaya, Kuala Lumpur, Malaysia...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In many research projects, cD...

The TripleMaster PCR System for three different amplifications

Christian Rohrer, Eppendorf AG Standard PCR components (Taq polymerase and reaction buffers) are used for the general amplification of relatively short DNA fragments (100 bp to roughly 2,000 bp). However, demanding...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

Eppendorf 5 Prime, Boulder, CO, USA The polymerase chain reaction (PCR*) is a powerful and sensitive technique used by scientists to amplify large quantities of DNA. Although the reaction mix and thermal cycling conditi...

BioRobot MDx standardize your automated protocols in clinical laboratories

The BioRobot MDx is the latest addition to the range of BioRobot workstations and is designed to meet the needs of scientists requiring standardized protocols in environments such as clinical testing laboratories....