High

Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line

midiprep kit Stratagene EF plasmid midiprep kit, a kit based on anion exchange chromatography, magneticbeads, and cesium ch...

High-Level Expression of Peanut Allergens Affected by Rare Codon Usage

Biochemical & Molecular Allergology, Research Center Borstel, Germany By using Stratagenes BL21-CodonPlus(DE3)-RIL cells * withextra copies of E. coli, argU, ileY, and leuW tRNA genes,it is possible to attain high-level expression...

Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression

Stratagene tissue arrayslides offer further refinement of tissue array technology through optimizedtissue fixation and processing procedures that preserve tissue morphology,antigenicity of pr...

Highest Possible Transformation Efficiencies for High-Throughput Applications

Convenient, time-saving 96Pack Gold competent cells streamline transformation procedures Julie Bonnardel Ronda Allen Bruce Jerpseth Sean Banks...

High-Throughput Cultivation of Bacterial Clones

Stratagene The tempest thermoshaker and a 96-deep-well round-bottom plate were used to cultivate bacterial clones in a...

Safe Production of High-Titer Retrovirus

Stratagene We describe a set of vectors that can be used with any MMLV-basedretroviral vector to produce viral supernatants that have titers that areconsistently greater than or equal to 10 cfu/ml...

New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter

hrGFP expression vectors for monitoring gene expression and localizing proteins in vivo New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter...

prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

Michael Borns Janice Cline Kris Connors RondaAllen Holly Hogrefe Stratagene RT-PCR systems are powerfulsystems that permit efficient, high-fidelity amplification of RNA in twoconvenient formats. The ProSTAR Ultra HF RT-PCR...

pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning

A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning Stratagene was cloned into the PC...

High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors

QuikChange XL: a highly versatile kit for site-directed mutagenesis oflarge plasmids Michael Borns Peter Vaillancourt Holly Hogrefe Jeff Braman Stratagene The QuikChange site-directed mutagenesis kit* characteristicallygenerates mutagenesis efficiencies exceeding 80%. We have created a new kit formutagenesis of large plasmid...

New Protocols for Isolating High- Molecular-Weight Genomic DNA

Stratagene Stratagene now offers updated protocols to isolate and purifyhigh-molecular-weight genomic DNA using the RecoverEase DNAisolation kit. New procedures include DNA isolation from blood, bo...

A Simple and Rapid Technique to Purify High-Quality Midiprep DNA

Stratagene A new technique is now available to quickly, easily, and reliably isolatehigh-purity plasmid DNA. The convenient spin-format eliminates the need forresin suspensions, gravity flow columns, and alcohol precipit...

High-Throughput Isolation of Total RNA

Stratagene 96 total RNA purification kitprovides a convenient method for simultaneous isolation of total RNA from up to96 cultured cell samples. Because RNA is bound to a solid support, there is noneed fo...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

Epitope Tagging in E. coli Katherine Felts Denise Wyborski John Bauer PeterVaillancourt Stratagene LIC cloning kit...

Yeast Protein Production System Features High Yields and One-Step Purification

The ESP system provides high-yield protein production in a eukaryoticorganism Quinn Lu * Bruce Jerpseth * Tim Sanchez * John C. Bauer * AlanGreener Stratagene Cloning Systems, Inc. provides novel opportunities for rapid, inexpensive and high-yield p...

Generate High-Quality, Directional Plasmid cDNA Libraries

Produce larger primary libraries with new plasmid library constructionkits Quinn Lu Marie Callahan Tanya Hosfield BarryNeiditch Alan Greener John C. Bauer Mike Kobrin Stratagene II,pcmv-script and pAD-GAL4-2.1 XR library construction kit...

High-Fidelity PCR with a Novel Polymerase Mixture

New polymerase mixture, TaqPlus Precision PCR system, developed forhighfidelity PCR Kirk B. Nielson * Janice Cline * Frances Bai * Daniel McMullan* Barbara McGowan * Holly Hogrefe Stratagene Cloning Systems, Inc. Stratagene announces the addition of the TaqPlus Precision PCR system,* to an expanding line...

Highly Active and Removable AffinityTagged Lambda Phosphatase

Dualspecificity Lambda Phosphatase useful for dephosphorylation serine,threonine or tyrosine residues Stratagene Cloning Systems, Inc. Stratagene's recombinant Lambda Phosphatase is a dualspecificity proteinphosphatase that has been produced as a fusion to Stratagene's novel affinitytag, the calmodulin...

hNT BlueNeurons Express b-Galactosidase at High Levels

-Galactosidase at HighLevels Layton BioScience, Inc. Gilroy, California Stratagene's hNT BlueNeurons express beta-galactosidase at h...

High-Titer Retroviral Vectors for Gene Delivery

High-TiterRetroviral Vectors for Gene Delivery Stratagene The high-titer retroviral vectors pFB and pFB-Neo can be used with anyMoloney Murine L...

Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance

Improve reliability of PCR reactions with Herculase enhanced DNApolymerase Stratagene enhanced DNApolymerase,* a novel enzyme formulation that providessuperior performance in both routine and demanding PCR applications. Previous...

High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications

Simultaneous purification of 96 PCR products Stratagene Phenogenex 96 PCR purification kit is a newmember of the StrataPrep product line. This kit offers a high-throughput formatfor purifying ultra-pure PCR product DNA from la...

High-efficiency Derivatives of the BL21 Series for Protein Expression

Melody Jerpseth Bruce Jerpseth Lisa Breister AlanGreener Stratagene Stratagene has created variants of BL21 competent cells, the new BL21-Goldseries. Epicurian Coli competent cells lack the EndA1 nuclease...

New Competent Cells for Highest Transformation Efficiencies

Stratagene Cloning Systems, Inc. Stratagenes new, high-efficiency competent E. coli cell linesignificantly improves the transformation efficiency of ligated DNA. EpicurianColi ultracompetent cells* yi...

High-Efficiency Transfections Achieved with New Low-Toxicity Reagent

Nonliposomal formulation yields high efficiencies in a variety oftransfection conditions Stratagene Introducing foreign DNA into eukaryotic cells requires high-potencytransfections, which demand superior quality transfection reagents that areefficient, have low toxicity, and wor...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Higher gDNA Concentrations with the Perfect gDNA Blood Mini Kit by Varying the Final Elution Volume

by Varying the Final Elution Volume Eppendorf 5 Prime, Boulder, Colorado Perfect gDNA Blood Mini Kit protocol recommend...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Higher gDNA Concentrations with the Perfect gDNA Blood Mini Kit by Varying the Final Elution Volume

by Varying the Final Elution Volume Eppendorf 5 Prime, Boulder, Colorado Perfect gDNA Blood Mini Kit protocol recommend...

High-throughput gene expression analysis to screen for anti-asthma drugs

Andreas Pahl, Uwe Oelmller,* and Susanne Ullmann Department of Pharmacology, University of Erlangen, Erlangen, Germany * QIAGEN GmbH, Hilden Germany Drug library screening based on gene expression analysis requires reliable and reproducible methods of RNA pu...

DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification

QIAGEN introduces the DirectPrep 96 Miniprep System for cost-effective, fully automated, high-throughput DNA purification from high-copy plasmids. Use of a novel lysis chemistry eliminates the need for a lysate clearing step. Plasmid DNA purification is performed using a single plate and a simple bindwashelute...

Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer

Mark Churchill , John Henninge, 1 Thermo Finnigan, Stafford House, 1 Boundary Park, Boundary Way, Hemel Hempstead, HP2 7GE, UK; 2 Aker University Hospital, Hormone Laboratory, Section for Doping Analysis,Trondheimsv...

Expand High FidelityPLUS PCR System

Explore New Horizons in Amplification Yield Combine High Yield, Accuracy, and the Prevention of Carry-Over Contamination Roche Applied Science, which pioneered the blending of thermostable DNA polymerases with the Expand High Fidelity and Expand Long Template PCR System...

Expand High Fidelity PCR System

for one-step RT-PCR amplification of up to 3 kb RNA targets Cat. No. 1 732 641 100 units Cat. No. 1 732 650 500 units Cat. No. 1 759 078 2500 units Unique enzyme blend of Taq and Pwo DNA Polymerases. The Expand High Fidelity PCR System...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

Kristi M. Miller, Tobi J. Ming and Ruth E. Withler, Pacific Biological Station,Department of Fisheries and Oceans, Nanaimo, B.C. V9R 5K6, Canada Introduction We describe a rapid and sensitive method for the detection of nucleotide sequence variation which can be used for largescale screening of population...

A highly sensitive ATP-luminescence assay using VICTOR2 with the dispenser option

In high sensitivity flash type luminescence assays the measurement is done just after dispensing the activating reagent or after a short delay time. Over the past years luminescence substrates have been improved towards providing more stable signal. These so-called glow type substrates allow microplates to be counted without dispensors. Ho...

TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization

Uses High Efficiency Fluorescence Polarization (HEFP) detection method Assays a broad range of tyrosine kinases Requires no separation or wash steps Available in a kit and in bulk packaging Screening for tyrosine kinase activity can be accelerated...

cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization

A Sensitive, Robust, Homogenous, and Rapid Assay Uses High Efficiency Fluorescence Polarization (HEFP) detection method Detects pmol amounts of cAMP Optimized for cell extract and enzyme preparations Requires no separation or wash steps Available in a kit and in bulk packaging ...

High Sensitivity, Wide Dynamic Range, Horseradish Peroxidase (HRP) ELISA Using the LMax Microplate Luminometer (MaxLine Application Note #41)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Immunoassays have traditionally employed colorimetric indicators. As such, multiple dilutions of samples were necessary to ensure that they fell within the dynamic range of the assay. Recently, chemiluminescent immunoassays, with gre...

Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing

Applied Science and Technology Department, Caliper Life Sciences, Inc., Hopkinton, MA 01748 The High Volume Head is one of four multi-channel liquid handling options available for the Sciclone Advanced Liquid Handler (ALH). The architecture of the device includes an arra...