Genes

Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library

Department of Cell Biology, Clevela...

Big Blue Rodent Cell Cultures for Mutagenesis Studies

Rat and mouse embryonic cell lines are useful for in vitro mutagenesisstudies Stratagene Cloning Systems, Inc. The Big Blue rat cell line and the Big Blue transgenic mouse embryoniccell line are useful for in vitro mutagenesis studies. These cell lines are partof the Big Blue transgenic rodent mutagenesis assay system...

High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors

QuikChange XL: a highly versatile kit for site-directed mutagenesis oflarge plasmids Michael Borns Peter Vaillancourt Holly Hogrefe Jeff Braman Stratagene The QuikChange site-directed mutagenesis kit* characteristicallygenerates mutagenesis efficiencies exceeding 80%. We have created a new kit formutagenesis of large plasmid...

RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

Stratagene Cloning Systems from the cDNAsequences of mismatch repair genes. These genes code for specialized proteinsthat participate in correcting areas of base mispairing in DNA. We have...

Randomize Gene Sequences with New PCR Mutagenesis Kit

GeneMorph PCR mutagenesis kit produces a unique mutational spectrum...

Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes

New GeneConnection discovery microarrays provide more than 4,000 uniquehuman cDNA sequences Stratagene introduces the first in a series of human cDNA microarrays on glass slides. These GeneConnection discovery microarrays which are based on the technology published by Schena et al., can be...

Listeria monocytogenes

Listeria monocytogenes 23074...

Listeria monocytogenes

Listeria monocytogenes 2307...

Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes

for Detecting Listeria monocytogenes Andrea Tanzer, Institute of Milk Hygiene, Milk Technology and Food Science, Austria Christian Rohrer, Eppendorf AG, Germany Introductio...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

Kristi M. Miller, Tobi J. Ming and Ruth E. Withler, Pacific Biological Station,Department of Fisheries and Oceans, Nanaimo, B.C. V9R 5K6, Canada Introduction We describe a rapid and sensitive method for the detection of nucleotide sequence variation which can be used for largescale screening of population...

Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments

RNA interference is a powerful technique for geneknockdown experiments in academic research and animportant tool for target identification and target validationin therapeutic research. A growing number of different cell lines are us...

Mutation detection for the K- rasand P16 genes

Mutations in the K-ras gene codon 12 region can lead to cancer, forexample of the colon, pancreas, liver, spleen, stomach or lungs. TheCDKN2A/P16 gene is a familial melanoma gene. Routine PCR and DNAsequencing methods can identify exactly which point mutation is present...

The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis

Drs. Frank Slack, Steven Johnson, and Helge Grosshans, and Ms. Kristy Reinert at Yale University, in collaboration with Ambion scientists, discovered that the microRNA (miRNA) family regulates the expression of C. elegans...

Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs

Ambion has developed a system for siRNA synthesis and purification based on in vitro transcription, the siRNA Construction Kit (patent pending). This kit produces transfection-ready siRNA at a fraction of the cost of chemical synthesis. Here, resear...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

Giles Cunnick and Wen Guo Jiang, Metastasis Research Group, UniversityDepartment of Surgery, University of Wales College of Medicine, Cardiff, UK Lymphangiogenesis refers to the formation of new lymphatic vessels. This process may occur in normal developing tissues or in tumors. In addition to sprea...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

Nikolaj Schmolker, Fintan van Ommen Kloeke and Gill Geesey, Departmentof Microbiology and Center for Biofilm Engineering, Montana State University,Bozeman, MT 59717-3980 Amplification of a region of the 16S rRNA gene of L. monocytogenes, Listeria innocua, and Listeria seeligeri by the polymerase chain reac...

Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor

M. Adams and R. Deadman GE Healthcare, Piscataway, NJ, US GE Healthcare offers a range of DNA amplification products to meet all genomic DNA and sequencing template preparation needs. A combination of these kits with the Genesis Freedom™ Robotic Sample Processor (RSP) from Tecan (Fig 1) creates a total solution for high-throu...

An Image-Based Assay of Endothelial Cell Tube Formation as a Model of Angiogenesis

Formation of new blood vessels is fundamental to the development of cancerous cell masses and has therefore been the focus of many drug screening and cell signaling research studies. Blood vessel development is a significant event in the development and growth of solid tumors, and is implicated in wound h...

A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity

Diana Ji, Stacy L. Deeds, and Edward J. Weinstein RNA interference methodologies have been utilized in small to large scale screening projects. The technology has allowed researchers to perform gene-silencing experiments in timeframes and target cells previously not possible. While siRNA screens have become fairly common, large scale screening wi...

The Genesis of Gendicine: The Story Behind the First Gene Therapy

In this BioPharmInternational exclusiveinterview,SiBionosfounder relates thescience andmanufacture of hiscompanys innovativecancer therapy....

Mirus provides low-cost way to modify genes

, a privately-held biopharmaceutical firm, has developed a way to bypass the conventional hurdles associated with producing modified...

UW links with Israeli school to locate genes

Superlink-...

James Thomson plans to stay in Madison to study genesis of stem cells

avoided talking politics in a public lecture on the eve of the presidential election, explaining instead the science behind his researc...

Predictive medicine: Genes indicate diseases before symptoms do

Today, doctors still follow the same basic paradigm for healthcare, said William Clarke, executive...

Study shows that genes can protect kids against poverty

MADISON - For children growing up poor, money isn't the only solution to overcoming the challenges of poverty. According to a new study, the genes and warm support received from parents also can buffer these children against many of the cognitive and behavioral problems for which poverty puts them at risk. The findings are published in the May issue of the journal Child Development....

Despite confinement, crop genes can spread fast to wild

MADISON - With the slim chance that farmers will stop planting crops containing genes from other organisms, researchers have started to develop strategies that trap these foreign genes, reducing the risk that they'll spread to wild relatives. But an investigation by scientists from the University of Wisconsin-Madison and the University of Minnesota-St. Paul shows that these containment s...