Fragment in Biological Technology

ForteBio Launches New Assay Capabilities for High-Sensitivity Protein Quantitation and Fragment Screening on Its Octet(R) Platform

MENLO PARK, Calif., May 27 /PRNewswire/ -- ForteBio(R), Inc., a leading supplier of label-free technology that accelerates the development of biotherapeutic and pharmaceutical products, today announced that it has launched two new assay modules - for high-sensitivity protein quantitation and fragm...

ESBATech Announces Successful Completion of Phase I Clinical Study for Lead Antibody Fragment in Ophthalmic Indications

Preclinical Pharmacokinetic Data to be Presented at the 7th International Symposium on Uveitis in Germany ZURICH, Switzerland, Sept. 8 /PRNewswire/ -- ESBATech AG, a leading developer of antibody fragment therapeutics, today announced that the company has successfully complet...

DNA Sequencing Equipment Market to Fragment and Grow as Competition, New Technologies Develop

NEW YORK, Aug. 24 /PRNewswire/ -- The DNA sequencing equipment market is expected to grow nearly 8% between 2006 and 2012, spiraling upward from $335 million to $528 million as competition intensifies and diversifies, according to DNA Sequencing Equipment and Service Markets, a new report by K...

Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

Introduction Amplified fragment length polymorphism (AFLP) analysis is a genetic mapping technique that uses selective amplification of a subset of restriction enzyme-digested DNA fragments to generate a unique fingerprint for a particular genome. First developed ...

Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3', 5' monophosphate and its validation for pharmacological screening at G protein-coupled receptors

Key words: cAMP • GPCR • enzyme fragment complementation • LEADseeker • assay miniaturization G protein-coupled receptors (GPCRs) are the largest family of transmembrane receptors and are responsible for the transduction of ...

Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping

Michaela Scigelova, Gary Woffendin; Thermo Finnigan, Hemel Hempstead, UK. Malcolm Ward, Helen Byers; Proteome Sciences, London, UK. Diane Hanger; Institute of Psychiatry KCL, London, UK. ...

Successful PCR amplification and subcloning of a GC-rich DNA fragment

Petra Majlingov, Paolo Paroncini, Silvia Svegliati, Michele Luchetti, and Armando Gabrielli Laboratorio di Medicina Molecolare, Istituto di Clinica Medica, Polo Didattico Scientifico, Ancona, Italy The formati...

Nuevolution Announces Worldwide Technology Cross-Licensing Agreement With GSK

... The company has developed Chemetics(R), a unique, patent protected hybrid of proven wet chemistry and molecular biology which represents the ultimate fragment based lead discovery technology. Chemetics(R) enables rapid synthesis and DNA-tagging of hundreds of millions of chemically diverse drug-like small mo...

ForteBio Launches Two Revolutionary High-Throughput, Label-Free Instruments: The Octet(R) RED384 and the Octet QK384

...armaceutical products, announced today that it has launched two next-generation instruments: the Octet RED384 for protein, peptide, small molecule and fragment screening; and the Octet QK384 for protein and antibody assays. The new instruments will debut this week at the 2009 American Association of Pharmaceu...

Odyssey Thera Announces Collaboration With Mitsubishi Tanabe Pharma Corporation, and Issuance of Core Patent in Japan

...s from a number of Mitsubishi Tanabe preclinical programs. Odyssey also announced the issuance of Japanese patent number JP 4262778, entitled "Protein fragment complementation assays for the detection of biological or drug interactions." "We look forward to applying our patented human cell analysis techn...

ChemoCentryx Identifies Novel Small Molecule C5aR Antagonist

... proteins that regulate certain types of inflammatory responses by the immune system. Fragments of these proteins, such as chemoattractant complement fragment C5a, work to recruit immune system cells including neutrophils and lymphocytes to the site of inflammation. The C5a receptor is highly expressed on i...

Zenobia Therapeutics Successfully Completes Initial Phase of Its Service Agreement With Syntonix Pharmaceuticals

...evelop our internal drug candidates," said Adam Mezo, Ph.D., Director of Chemistry at Syntonix. About Zenobia Zenobia provides a commercial fragment library and access to their structural biology, crystallography and fragment-based lead discovery expertise through partnerships, consulting and colla...

Nuevolution Announces Progress in Its Collaboration With Merck & Co. Inc.

... The company has developed Chemetics(R), a unique, patent protected hybrid of proven wet chemistry and molecular biology which represents the ultimate fragment based drug discovery technology. Chemetics(R) enables rapid synthesis and DNA-tagging of hundreds of millions of chemically diverse drug-like small mo...

Heptares Secures GBP21 Million in Series A Fund Raising to Accelerate Development of Pipeline of Small Molecules Against High-Value GPCR Drug Targets

...hen apply contemporary drug discovery approaches, such as crystallisation and structure-based design, biophysical analysis of ligand interactions, and fragment screening to stabilised GPCRs. This unique approach is expected to radically improve the chances of finding drugs to previously intractable targets an...

Frost & Sullivan Recognizes ESBATech's Innovation in Developing Antibody Fragments for Biologic Therapeutics

...ze of ESBATech's proprietary single-chain antibody fragment therapeutics addresses and overcomes many of the c...on of all 1.5 million naturally occurring antibody fragment frameworks resulted in highly-stable, single-chain...ecombinant humanized scFv non-aggregating antibody fragment being evaluated in treating a number of indication...

Novel Mass Spectrometry-based Assay Reveals Degradation and Low Levels of Bioactive B-type Natriuretic Peptide (BNP) in Patients with Heart Failure

...m Intrinsic Bioprobes. BNP is synthesized as a 108 amino acid propeptide and proteolytically processed to release an inactive 76 amino acid N-terminal fragment and bioactive mature BNP 1-32 into the bloodstream. "This is the first assay capable of detecting and quantifying BNP 1-32 alone. Furthermore, the ass...

Caliper Life Sciences and DiscoveRx Announce Availability of PathHunter eXpress Reagents for GPCR Assays

...any headquartered in Fremont, California, with an additional office in Birmingham, England. The Company pioneered the use of Beta-Galactosidase enzyme fragment complementation in biochemical and cell based assays for discovery research, and holds extensive intellectual property in this area. DiscoveRx is dedi...

Bavarian Nordic A/S - Interim Report for the Period 1 January to 30 June 2008

...ess HER2. Patients are vaccinated 3 times, at 3 week intervals, with the MVA-BN(R) viral vector which has been engineered to express the extracellular fragment of human HER2. By July 2008 enrolment in the studies was completed. No drug-related severe adverse events have been reported thus far. The vaccine h...

U.S. Genomics Awarded $9.1 Million Contract for Development of Advanced Biosensor by the U.S. Department of Homeland Security

...e positive rates. Using a universal reagent set that can detect and identify pathogens, the technology produces a genetic signature unique to each DNA fragment in the sample, and also identifies the organism from which the DNA originates. Unlike other detection technologies, U.S. Genomics' detection platform ...

SGX Pharmaceuticals Submits Investigational New Drug Application for SGX393

...y and development programs forward," said Mike Grey, CEO of SGX Pharmaceuticals. About SGX393 SGX393 was discovered by SGX utilizing FAST(TM), its fragment based approach to drug discovery. SGX393 initially fell within the purview of the Company's collaboration with the Novartis Institute for Biomedical R...

Nuevolution Announces Research and Development Collaboration with Merck & Co. Inc.

... The company has developed Chemetics(R), a unique, patent protected hybrid of proven wet chemistry and molecular biology which represents the ultimate fragment based drug discovery technology. Chemetics(R) enables rapid synthesis and DNA-tagging of hundreds of millions of chemically diverse drug-like small mo...

New molecular imaging techniques may lead to advances in disease treatment

...act as reporter probes. Cell culture and animal studies demonstrated intense and highly specific uptake of the probes in cells expressing the antibody fragment on the cell surface. These data indicate that antibody-based reporter genes represent a promising new platform for the development of new reporter gen...

Northwestern chemist investigates lost reds in Homer painting

...working to identify those used in Homer's painting as well as in a variety of textiles, such as a 16th century carpet from Istanbul and a rare textile fragment from Peru, dated from 800 to 1350 A.D. "Our research provides an entirely new window onto the analysis of artworks," said Van Duyne. "There's a br...

Nuevolution Announces Drug Discovery Alliance With Lexicon Pharmaceuticals

... The company has developed Chemetics(R), a unique, patent protected hybrid of proven wet chemistry and molecular biology which represents the ultimate fragment based drug discovery technology. Chemetics(R) enables rapid synthesis and DNA-tagging of hundreds of millions of chemically diverse drug-like small mo...

Caliper Life Sciences and DiscoveRx Collaborate to Offer Solutions for Cell-Based GPCR Profiling and Lead Optimization

...any headquartered in Fremont, California, with an additional office in Birmingham, England. The company pioneered the use of Beta-Galactosidase enzyme fragment complementation in biochemical and cell based assays for discovery research, and holds extensive intellectual property in this area. DiscoveRx is dedi...

New Fragment-based Drug Discovery Platform Announced Between IOTA Pharmaceuticals Ltd, Cambridge (UK), and Beactica AB, Uppsala (Sweden)

...D-based lead discovery and optimization platform. The IOTA-Beactica platform couples comprehensive, site-directed FBDD libraries to state-of-the-art fragment and lead screening using surface plasmon resonance (SPR) biosensor technology, enabling the rapid progression of new discovery programs from concept t...

Genetic Engineering & Biotechnology News reports on growing use of cell-based assays

...es protein-protein interactions. DiscoveRx created a new family of cell-based assays for the detection of protein-protein interactions using an enzyme fragment complementation assay. This system uses an enzyme acceptor, an inactive alpha-galactosidase enzyme that binds to its enzyme donor forming an active en...

ESBATech's Antibody Fragments Demonstrate Penetration into the Eye with Topical Delivery via Eye Drops

...e/ -- ESBATech AG, a leading developer of antibody fragment therapeutics, today announced that it has achieved...our, retina, and choroid. ESBATech's lead antibody fragment ESBA105 is a TNFa antagonist that is scheduled to ...ular size. The primary advantage of human antibody fragment is their ability to penetrate tissues much better ...

Viventia Biotech to Explore Strategic Alternatives - Reports Positive Pivotal Phase III Interim Efficacy Data

...s, unlike the relatively high toxicity associated with other forms of intravesical therapy, including BCG. VB6-845 VB6-845 is a humanized antibody fragment targeting EpCAM fused with the Company's proprietary deimmunized form of the cytotoxic protein Bouganin, and is designed for systemic use against meta...

Presentation of Phase IIa Results for Cytos Biotechnology's Hypertension Vaccine at the American Heart Association Scientific Sessions 2007

... infarction: commonly known as a heart attack; is a disease state that occurs when the blood supply to a part of the heart is interrupted. Peptide: a fragment of a protein comprised of two or more amino acids. Phase IIa: clinical trial that examines a new drug candidate's safety and exploratory efficacy and...

Court bars patent protection for certain gene fragments

...ions useful. At issue was whether certain gene fragment sequences were useful. Most notably whether the ge...least contentious hurdle to overcome, but for gene fragment patent applications the standard has now been heig...claim describing a set of five ESTs that encodes a fragment or entire maize protein. Neither the genes that en...

Separating science fiction from reality in the human cloning debate

...for years. The process known as DNA cloning, molecular cloning or gene cloning has been used widely since the 1970s. It involves the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid. The DNA of interest can then be propagated in a forei...

UW compound counters Avian flu

...try According to the Journal of Virology report, the UW-Madison researchers have found a flu-fighting peptide, a small protein molecule which is a fragment of a larger human protein. The peptide blocks the influenza virus from attaching to and entering the cells of its host, which prevents it from replica...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

...multiple displacement amplificatio . Proc. Nat. Acad. Sci., USA, 99 : 5261 15. Aviel-Ronen S., et al., (2006) Large fragment Bst DNA polymerase for whole genome amplification of DNA from formalin-fixed paraffin-embedded tissues . BMC Genomics. 7 : 312 16....

140-Fold Increase of Protein Achieved in Pichia Fermentation

... yields by the researchers thus far had been 1.4 mg/L of a throm-bomodulin fragment when culturing Pichia in shake flasks. By switching to a BioFlo 3000 ...ient feeding and oxygen supplementation. Production of the thrombomodulin fragment in the fed-batch studies reached an average of 200 mg/L, a 142-fold incre...

Glossary for The BioPharm Guide to Fermentation and Cell Culture

...are from the same person. The technique of analyzing restriction fragment length polymorphism (RFLP) is called DNA typing or DNA fingerpri...chorage-dependent cells from their attached substratum. tryptic fragment analysis Quantitating the resultant fragments caused by tryptic ...

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

... 143-148 4. Clare, J.J., F.B. Rayment, S.P. Ballantine, K. Sreekrishna and M.A.Romanos, 1991. Highlevel expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. BIO/TECHNOLOGY, Vol 9, 455-460 5. Cregg, J.M., T....

LC-MALDI and MALDI-MS/MS for the Analysis of Complex Peptide Mixtures

...(Low Mass Zoom (LMZ)) which can be used to enhance fragment ion resolution for low mass fragment ions, e.g. diagnostic ions used for relative quant...ng technology. Using the 114 and 117 reagents, the fragment ion resolution obtained in MS/MS mode is suffi c...

Denaturing HPLC (DHPLC)

...sation of DHPLC, requires the generation of a melting profile for each PCR fragment to determine its optimal temperature for analysis. Usually, the low...ute from the column. There are columns on the market for performing fragment analysis, they include the DNASep Column, the Helix DVB column and the Zor...

Analysis of a Verapamil Microsomal Incubation using Metabolite ID and Mass FrontierTM

... parent drug (bottom right) reveals a large common fragment ion at m/z 303. This supports the conclusion that ...hylated by the observation that the mass 165 MS 2 fragment of verapamil is unshifted in the demethylated meta...lite. The same logic applied to the mass 260 MS 2 fragment eliminates sites 1 and 2. This leaves site 3 as th...