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Fluorescent in Biological Technology

A breakthrough toward industrial production of fluorescent nanodiamonds

The laboratory Structure - Activit of Normal & Pathologic Biomolecules SANPB , Inserm / UEVE U829 (Genopole Evry, France) in collaboration with the Material Centre of Mines-ParisTech (Evry, France), the NRG - UMR 5060 CNRS / UTBM (Technology University of Belfort-Montbliard) and the Physic Instit...

LI-COR Introduces the Odyssey(R) Two-Color NIR Fluorescent Protein Molecular Weight Marker

LINCOLN, Neb., Oct. 14 /PRNewswire/ -- LI-COR Biosciences introduces a new, novel protein molecular weight marker for detection on the Odyssey(R) Infrared Imaging system. The Odyssey Two-Color Protein Molecular Weight Marker is pre-stained and fluoresces in the 700 and 800 nm channels of the O...

Explosion Proof Fluorescent Lights Keep Paint Spray Booths and Oil Rig Workers Safe

OSHA requirements mandate that workers exposed to flammable vapors or dusts be protected by UL certified lights that have an explosion proof class rating, and Magnalight.com now has a significant offering of these lights available for oil rig workers and paint spray booth operators. ...

Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)

Simon Lydford 1 and Thomas Giller 2 1 Molecular Devices Limited and 2 Axovan Ltd. INTRODUCTION GFP (Green Fluorescent Protein) is an extremely stable protein of 238 amino acids produced by the jellyfish Aequorea victoria 1 that fluoresces in the lower green r...

measurement of green fluorescent proteins and a red fluorescent protein in live cells in the Analyst GT multimode reader

Evelyn McGown, Ph.D., Jinfang Liao, M.D., Ph.D., Mary Kassinos, Irina Osetinsky and Jayne Hesley, Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. Introduction Fluorescent proteins have become enormously popular as tools for monitori...

Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers

Abstract Single-strand conformation polymorphism (SSCP) analysis on the ABI PRISM 3100 and the 3100 Avant Genetic Analyzers provides fast, easy detection of small variations in DNA sequences, including singlebase substitutions, insertions, or deletions. SSCP An...

Minimizing Photobleaching of Blue Fluorescent Protein (BFP) using the Varian Cary Eclipse Fluorescence Spectrophotometer

Paul Gavin*, Mark Prescott*, Ph.D, Daren J. Fyfe#, Ph.D and Jeffrey J. Comerford#, Ph.D * Department of Biochemistry and Molecular Biology, Monash University, Clayton campus, Victoria 3800, Australia # Technical Assist...

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae : Detection in vivo using the Varian Cary Eclipse Paul Gavin# and Mark Prescott # , Ph.D Daren J. Fyfe, Ph.D * # Department of Bi...

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

Vasker Bhattacherjee, Partha Mukhopadhyay, Saurabh Singh, Robert M. Greene and M. Michele Pisano, University of Louisville Birth Defects Center, Department of Molecular, Cellular and Craniofacial Biology, Louisville, KY 40202 Abstract This application note describes a Las...

Performance of fluorescent dyes with LEADseeker Multimodality Imaging System

Key words: fluorescence • LEADseeker • Cy • Alexa Fluor • TAMRA • Brij Flexible and scalable uHTS programs demand that organizations be able to select the most appropriate or cost-effective fluorescent reagents for t...

Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting

O. Rönn 1 , M. Landström 2 , A. Edman-Örlefors 1 , and S. Edlund 1 1 GE Healthcare, Uppsala, Sweden 2 Ludwig Institute for Cancer Research, Uppsala, Sweden ECL Plex™ Western Blotting Detection System ...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

Key words: ECL Plex • Ettan DIGE Imager • fluorescent Western blotting • multiplex detection • dynamic range The Ettan™ DIGE Imager is fully compatible with the ECL Plex™ system, reaching the same levels of sensitivity, ...

Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer

Contributed by Jeff Wilson, and Andrew Segal, M.D., Genitrix, LLC, Cambridge, MA Introduction The Green Fluorescent Protein (GFP) derived from Aqueorius victoria has recently become a popular choice as a reporter gene. GFP can be used...

Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer and the Cell Fluorescence LabChip kit can be used to determine the efficiency of transfection of mammalian cells using green fluorescent protein (GFP) as a ...

Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells

Comparison of liposome-based transfection reagents D.J. Lowrie Jr. Department of Cell Biology, Neurobiology, and Anatomy University of Cincinnati College of Medicine, Cincinnati, OH 45267 Transfection of mammalian cell lines is enhanced by the use of liposome-based reag...

Genetically engineered bacteria compute the route

...se by the larger synthetic biology community, including the newly split red fluorescent protein and green fluorescent protein genes," said Jordan Baumgardner, recent graduate of Missouri Wester...

NYU physicists find way to explore microscopic systems through holographic video

...another moleculeor tagthat binds to the target molecule. This tag molecule, time-consuming and costly to produce, is typically identified by making it fluorescent or radioactive. The holographic imaging technique, with its magnification and recording capabilities, allows researchers to observe molecular-scal...

BRAHMS, Cezanne and Lumiphore Announce Partnership for Diagnostic Tests

...re Inc., a biotechnology leader in new proprietary fluorescent metal-lanthanide technology for use in high-value ...ey . Lanthanides are exceptional due to their long fluorescent lifetime delays that enable time gating and a dram...nt time resolved fluorescence (TRF) reporters, and fluorescent resonance energy transfer (FRET) donors in proximi...

A Worldwide Milestone in Cell Analysis - 40 Years of Flow Cytometry

...ll size, even in nanometer range, are detected. The cells are marked with specific reagents and excited by one or more laser, consequently the emitted fluorescent signals are collected. With the obtained data, cells can be characterized, classified, quantified and sorted. For HIV monitoring, accompanying the ant...

Regular light bulbs made super-efficient with ultra-fast laser

...light as bright as a 100-watt bulb consume less electricity than a 60-watt bulb while remaining far cheaper and radiating a more pleasant light than a fluorescent bulb can. The laser process creates a unique array of nano- and micro-scale structures on the surface of a regular tungsten filamentthe tiny wire in...

MIT, BU engineer cellular circuits that count events

...DNA, setting up the third invertase gene for transcription. The output of the system can be determined when an output gene, such as the gene for green fluorescent protein, is inserted into the cascade and is produced after a certain number of inputs or by sequencing the cell's DNA. This circuit could in theor...

Ambry Genetics Announces the Addition of Array CGH Services Through Partnership With Baylor College of Medicine

...omal imbalances with a single laboratory test consisting of multiple probes targeting virtually all disorders detected by traditional cytogenetics and fluorescent in situ hybridization (FISH). Array CGH is designed for patients with unexplained developmental delay, autism spectrum disorders, dysmorphic features,...

China Medical Technologies Receives SFDA Approval for Its Bladder Cancer FISH Probe

...evice company that develops, manufactures and markets advanced in-vitro diagnostic (IVD) products using Enhanced Chemiluminescence (ECLIA) technology, fluorescent in situ Hybridization (FISH) technology and Surface Plasmon Resonance (SPR) technology to detect and monitor various diseases and disorders. For more ...

VisEn Launches Next-Generation Fast ('Fluorescence Activatible Sensor Technology') Agent Platform for Expanded Performance in Imaging Disease Biomarkers In Vivo

...ated biomarkers and drug response in vivo . Newly developed by VisEn Medical, FAST imaging agents are smaller molecule, biocompatible, activatible fluorescent agents that combine increased biomarker specificity with accelerated pharmacokinetics for advanced measurement and monitoring of key disease biomarker...

SeqWright to Invest in LaserGen's Novel Lightning Terminators(TM)

... Simply stated, Lightning Terminators(TM) are better than nature. All reversible terminators required a cleavage step to remove the terminating and fluorescent dye groups. Upon cleavage, however, Lightning Terminators(TM) restore the incorporated nucleotide back to its natural state. This distinguishes th...

Researchers develop new way to see single RNA molecules inside living cells

...ng the need for synthetic RNA or a large number of fluorescent molecules. The fluorescent probes developed at the Georgia Institute of Techn...udy, the probes produced by attaching a few small fluorescent molecules called fluorophores to a modified nuclei...

China Medical Technologies Senior Management Purchased Company Shares

...evice company that develops, manufactures and markets advanced in-vitro diagnostic (IVD) products using Enhanced Chemiluminescence (ECLIA) technology, fluorescent in situ Hybridization (FISH) technology and Surface Plasmon Resonance (SPR) technology to detect and monitor various diseases and disorders. For more ...

Rice rolls out new nanocars

...arget to be on a conductive substrate. Not so with fluorescent imaging. A paper on the new research published ...id Link of the team's initial success in attaching fluorescent dye trailers to the nanocars. "We were worrying ab...built that would eliminate the drag created by the fluorescent trailer. He speculated that putting six wheels ins...

New sperm shaker to improve IVF success

...hey do not give its DNA status. There are established tests for sperm DNA quality but they work by cutting the cells in half and tagging them with fluorescent dye a process that kills the sperm and renders it useless. This new process does not destroy the sperm, so if it is found to have good DNA quality, i...

Researchers: Molecular forklifts overcome obstacle to 'smart dust'

...traditional assays. But then, activated by a flash of light, molecular shuttles start pushing the forklifts into a second zone, where they load aboard fluorescent particles, or tags. They move their cargo to the third zone, at the edge of the circle. There, over several hours, they crowd against each other, accu...

Easy assembly of electronic biological chips

...quences complementary to the three virus-specific sequences on the nanowires. Because they tagged the complimentary DNA with three differently colored fluorescent dyes, the attached DNA showed that the wires were in the proper places. The researchers believe that their assembly method is extremely flexible, ca...

New label-free method tracks molecules and drugs in live cells

...g cells and tissues without the use of any kind of fluorescent labeling. The technique, based on stimulated Ra...of real-time, three-dimensional bioimaging free of fluorescent labels that can hinder many biological processes. ... jellyfish protein first discovered in 1962, green fluorescent protein (GFP), is now used extensively as a label ...

Duke researchers coax bright white light from unexpected source

... lighting with better characteristics than current fluorescent ones," said Everitt, who also works with Foreman a...ies' bottoms also has major safety advantages over fluorescent bulbs, which happen to contain toxic mercury. "If a fluorescent bulb gets broken in the course of battle, it expos...

New technique allows simultaneous tracking of gene expression and movement

... Flies expressing green fluorescent protein (GFP) in their retina cells or other tissu...thern California, Los Angeles, who carried out the fluorescent experiments in Drosophila flies. When the flies ar...l activity, behavior and mortality". The green fluorescent protein gene is isolated from the jellyfish Aequor...

Martek Announces Fourth Quarter and FY 2008 Financial Results

... (8,450) 6,575 Expenditures for property, plant and equipment (9,785) (8,279) Proceeds from sale of fluorescent detection products business - 900 Repurchase from sale-leaseback transaction ...

60 LED Lights Enhance Magnifying Lamp for Assembly, Engineering, Inspection or Research

...;ProVue LED adds work pace efficiency, users tell us."     Additional benefits arise from reduced energy use, compared to fluorescent or incandescent lamps, as well as improved color quality and consistency. LEDs are long-lasting and eliminate disposal risks. The magnifying...

China Medical Technologies Completes the Development of EGFR Molecular Diagnostic Kits

...l device company that develops, manufactures and markets advanced in-vitro diagnostic products using Enhanced Chemiluminescence (ECLIA) technology and fluorescent in situ Hybridization (FISH) technology, to detect and monitor various diseases and disorders, and products using High Intensity Focused Ultrasound (H...

Amgen Foundation Announces $2.5 Million Expansion of Amgen Scholars Program to Europe

...example, Dr. Martin Chalfie of Columbia University, one of the 2008 Nobel Prize recipients in Chemistry for the discovery and development of the green fluorescent protein, GFP, mentored an Amgen Scholar in his lab at Columbia. Amgen Foundation's 10 host universities for the Amgen Scholars U.S. Program are Cali...

SENOMYX ANNOUNCES THIRD QUARTER 2008 FINANCIAL RESULTS

...es," Snyder added. Dr. Tsien was cited for his seminal contributions regarding understanding the chemistry of the fluorescence properties of the green fluorescent protein, GFP, and other members of the GFP family. The modified GFPs developed by Dr. Tsien provide the means to analyze protein-protein interactions ...

Sysmex America Set to Launch New XT-Series(TM) Parameters

...ematology Analyzers provides a quantitative immature granulocyte count for metamyelocytes, myelocytes and promyelocytes by utilizing Sysmex's patented fluorescent flow cytometry technology. This patented technology, used to separate white blood cells into distinct cell clusters, reduces labor-intensive manual WB...
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