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Automated Fluorescent-Tag Cycle Sequencing
Stratagene Cloning Systems, Inc. *,# with Hot Top Assembly can be used to perform fluorescent-tag cyclesequencing using the ABI PRISM dye terminator cycle sequencingr...hrGFP expression vectors for monitoring gene expression and localizing proteins in vivo New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter...
Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
Department of Cell Biology, Neurobiology, and Anatomy University of Cincinnati College of Medicine, Cincinnati, OH 45267 Transfection of mammalian cell lines is enhanced by the use ofliposome-based reagents. Here we compare the transfection e...Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
Xiuyuan Hu Beti Belachew Lingyu Chen HaoqiangHuang Jason Zhang Stratagene can distinguish human DNA specimens that differ by a few nucleotides or only asingle nucl...Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit
Although the <A href="http://www.ambion.com/catalog/CatNum.php?1632"> siRNA Labeling Kits were developed for labeling siRNA, they can be readily adapted for labeling long dsRNA. The following protocol can be used for this application. See "<A href="#visualizing">Visualizing In...Receptor Binding Assays using Fluorescence Polarization Detection: the [FP]2TM Characterized Tracers
. The process is typically highly automated and can provide screening rates over 100,000 samples in a 24 hour period. To achieve this, assays must be simple, p...Evelyn McGown, Ph.D., Jinfang Liao, M.D., Ph.D., Mary Kassinos, Irina Osetinsky and Jayne Hesley, Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. They can be cloned in a diverse range of cells and org...
Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between synthesis and degradation. Whereas prot...Uses High Efficiency Fluorescence Polarization (HEFP) detection method Assays a broad range of tyrosine kinases Requires no separation or wash steps Available in a kit and in bulk packaging Screening for tyrosine kinase activity can be accelerated...
cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization
A Sensitive, Robust, Homogenous, and Rapid Assay Uses High Efficiency Fluorescence Polarization (HEFP) detection method Detects pmol amounts of cAMP Optimized for cell extract and enzyme preparations Requires no separation or wash steps Available in a kit and in bulk packaging ...Evelyn McGown, Ph.D. and Anna Lam, B.S. Molecular Devices Corporation, 8/99 Modern assays for proteolytic activity typically use synthetic chromogenic or flu-orogenic peptide substrates. Enzyme selectivity is obtained by choosing a peptide sequence uniquely recognized by the catalytic site of a particular enzy...
Anne T. Ferguson, Ph.D. Molecular Devices Corporation This application note describes the use of the SPECTRAmax Viability/Cytoxicity Assay Kit for animal cells. This assay uses two fluorescent dyes, calcein AM (cal...
1 GFP (Green Fluorescent Protein) is an extremely stable protein of 238 amino acids produced by the jellyfish that fluoresces in the lower green region of the visible spectrum....
October 1999 Molecular beacons are fluorogenic oligonucleotide probes developed by Kramerand associates to detect specific nucleic acids in homogeneous solutions. They are single-stranded oligonucleotides that exist in a hairpin shape because of thecomplementary arms at the ends which bind together to form a stem....
HIGHER THROUGHPUT IMPROVED DATA QUALITY RESCUES SUB-OPTIMAL ASSAYS IMPROVED FUNCTIONALITY IN...
Calcium Flux Assays using the LabChip 3000
The LabChip microfluidic assay accesses test compounds from a micropl...Medical College lands $8.1M grant to detect bird flu, bioterror agents
The grant, which was awarded by the <a href="http...Prodesse gets new funding; improves flu assay
The company reported that a group of its core investors, plus three new accredited individuals, have provided an additional $850,000 in funding. The offering is com...This Application Note describes how the Agilent 2100 bioanalyzer andthe Cell Fluorescence LabChip kit can be used to determine the efficiencyof transfection of mammalian cells using green fluorescent protein(GFP) as a reporter molecule. Transfection of CHO-K1 cells with...
Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
Gastric infection with Helicobacterpylori (H. pylori) is the majorcause of chronic active gastritisand is associated with the pathogenesisof peptic ulcer and gastriccarcinoma. Recent studies using amultiplex PCR (mPCR) basedapproach distinguished differental...By Susan Catalano, Ph.D., Acumen Pharmaceuticals, Sylvia de Bruin, Joe Bosworth, Ph.D., Neal Gliksman, Ph.D., Kimberly Best, Ph.D. and Paula Rickert, Ph.D., Molecular De...
The SPEX FLUOROLOG spectrofluorometer is capable of detecting sub-picomolar ( < 1012 M) fluorescein according to the conventions established by the ASTM Committee E-13 on Molecular Spectroscopy (ANSI/ASTM E579-76). Other commercial manufacturers of spectrofluorometers specify their Minimum Detectable Concentration (MDC) somewhat differently. This technical note demon...
Maximize Viral RNA Yield from Biological Fluid
Ambions magnetic bead-based MagMAX Viral RNA Isolation Kit is optimized for isolating viral RNA from biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. RNA isolation from cell-free samples presents some unique challenges compared to RNA...Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect
Mike Byrom, Vince Pallotta, David Brown*, and Lance Ford* Ambion, Inc. * Corresponding authors <A href="mailto:dbrown@ambion.com">dbrown@ambion.com , <A href="mailto:lford@ambion.com">lford@ambion.com...Genomic DNA Isolation From 1 ml of Body Fluid
Catalog #732-6340 Expected yield range: 250 g DNA (15-ml tube prep) 1. Add 1 ml body fluid (e.g., cerebrospinal fluid, plasma, saliva, serum, sputum, synovial fluid, urine, whole blood, milk) to a ster...Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
Wenda Ribeiro and Margaret Saha, Department of Biology, College of William and Mary, Williamsburg, Virginia 23187 DNA sequence analysis is an integral part of genetic research. Recently, the use of automated sequencers has become increasingly more common in small research laboratory sett...Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
Matthew W. Fields, Section of Microbiology, Cornell University, Ithaca,New York 14853 Biologists frequently monitor the effect of environmental stimuli on living organisms by quantitating mRNA levels. The relative abundance of mRNA is typically determined with probes and hybridization blots, b...Total RNA Isolation From 500 l Body Fluid
Catalog #732-6370 1. Vigorously add 2.5 ml cell lysis solution to an Oak Ridge centrifuge tube (or other tube rated for high speed) containing 500 l body fluid (e.g., cerebrospinal fluid, plasma, sali...Andrew Tingey, Reading Scientific Services Ltd., The Lord ZuckermanResearch Centre, Whiteknights, PO Box 234, Reading RG6 6LA, UK One of the most topical issues in the food industry is the use of genetically modified organisms (GMOs) in agriculture and food production. Demands from consumers, particu...
Nicholas N. Lyssenko and Mark L. Tucker, Soybean and Alfalfa Research Lab,USDA, ARS, Bldg. 006, BARC-West, Beltsville, MD, USA 20705 In 1987 Jefferson et al. described the use of β-glucuronidase (GUS) as a marker to study gene expression in plants. Expression of GUS activity in plant tissu...
Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
Contributed by Jeff Wilson, and Andrew Segal, M.D., Genitrix, LLC, Cambridge, MA The Green Fluorescent Protein (GFP) derived from Aqueorius victoria has recently become a popular choice as a reporter gene. GFP can be used, for example, to study promoter function and to track the expression, loc...Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
CSIRO Tropical Agriculture, Brisbane, Queensland The Green Fluorescent Protein (GFP) from Aequorea victoria is a useful reporter gene in higher organisms, including plants and animals. The fluorescence emitted is easy to det...ECL Plex • Ettan DIGE Imager • fluorescent Western blotting • multiplex detection • dynamic range The Ettan™ DIGE Imager is fully compatible with the ECL Plex™ system, reaching the same levels of sensitivity, linearity, and dynamic range as the Typhoon™ scanner. Similar results are also obt...
Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
<P >O. Rönn ECL Plex™ Western Blotting Detection System can detect proteins at pi...Performance of fluorescent dyes with LEADseeker Multimodality Imaging System
Flexible and scalable uHTS programs demand that organizations be able to select the most appropriate or cost-effective fluorescent reagents for the task. This application note describes experiments to assess...The cell cycle is of key importance to many areas of drug discovery. On the one hand this fundamental process provides the opportunity to discover new therapeutic targets for anti-cancer agents, and on the other hand drugs and targets in other therapeutic areas must be tested for undesirable effects on the cell cycle. <P >The Cell Proliferation Fluorescence Kit is...
Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
This application note describes a Laser Capture Microdissection (LCM) method for the isolation of cells expressing en...Simultaneous Screening of 23 Drugs of Abuse in Oral Fluid Using an LC/MS/MS Method
They are not as commonly available for novel sample matrices such as saliva as...Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae : Detection in vivo using the Varian Cary Eclipse Department of Biochemistry and Molecular Biology, Monash University,...
fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse Department of Biochemistry and Molecular Biol...
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(Date:7/24/2008)... Well inside the Arctic Circle, scientists have fo...as ever seen before. The cluster of five vents on...ing water as hot as 570 F., Dissolved sulfide mi...cold of the deep sea have, over the years, accumul...ost massive hydrothermal sulfide deposits ever fou...
(Date:7/24/2008)...ection due to a virus called cytomegalovirus (CMV)...sed immune systems, can also affect hospital inten...ems, University of Washington researchers have fou...ospital and intensive-care unit (ICU) stays indepe...lished July 23 in JAMA , the Journal of the Amer...
(Date:7/24/2008)...ine, Calif., July 24, 2008 Adult stem cells origi...only believed, and with proper stimulation they ca... disease or injury, a study by UC Irvine scientist...true stem cells in the mammalian brain are the epe... and spinal cord, rather than cells in the subvent...
(Date:7/24/2008)... YORK, July 24, 2008 In the life of a cell, the r...is fated to pause and repair itself, commit suicid...er. In a new study, published in the July 25th iss...nter have identified a way that cells respond to D...for disposal. The finding points to a new pathway ...
Breaking Biology News(10 mins):Scientists break record by finding northernmost hydrothermal vent field 2Scientists break record by finding northernmost hydrothermal vent field 3CMV infections affect more than just patients with compromised immune systems, researchers find 2Adult stem cells activated in mammalian brain 2Adult stem cells activated in mammalian brain 3A new cellular pathway linked to cancer is identified by NYU researchers 2Trust for Americas Health on New HHS Influenza Pandemic Draft Guidance 21100 1Trust for Americas Health on New HHS Influenza Pandemic Draft Guidance 21100 2NuVasive to Present at Goldman Sachs 29th Annual Global Healthcare Conference 21098 1NuVasive to Present at Goldman Sachs 29th Annual Global Healthcare Conference 21098 2NuVasive to Present at Goldman Sachs 29th Annual Global Healthcare Conference 21098 3MultiVu Video Feed 3A Study in the New England Journal of Medicine Shows High Blood Pressure Drug Tekturna 28R 29 Reduces Key Marker of Kidney Disease 21096 1MultiVu Video Feed 3A Study in the New England Journal of Medicine Shows High Blood Pressure Drug Tekturna 28R 29 Reduces Key Marker of Kidney Disease 21096 2MultiVu Video Feed 3A Study in the New England Journal of Medicine Shows High Blood Pressure Drug Tekturna 28R 29 Reduces Key Marker of Kidney Disease 21096 3Echocardiography Lab at Presbyterian Hospital of Dallas Achieves Prestigious Reaccreditation 21094 1Echocardiography Lab at Presbyterian Hospital of Dallas Achieves Prestigious Reaccreditation 21094 2(Date:7/25/2008)...ON, July 25 /PRNewswire/ -- The Elekta Family of C... treatment solutions at the 2008,American Associat...g,demonstrating how the joint forces of Elekta, I... therapy planning and delivery solutions that,stre...perience for,patients, as well as enhance clinicia...
(Date:7/25/2008)...ds fluid back-up in esophagus can cause immune sys...ay News) -- The first evidence linking gastroesoph...scovered by Duke University Medical Center researc...ns was first noted in the 1970s, and since then st...rcent of asthma patients also experience GERD symp...
(Date:7/25/2008)... The Retina Group of New York will...ted macular degeneration especially designed for s...e Public Library at 116 Merritts Road, Farmingdale.... Attendance is free and refreshments will be prov...6) 939-6100. , Hicksville...
(Date:7/25/2008)... This donation will be used to fac...ve services for children , ... ( www.ivci.com ), a leading integrator of enterp...tion video conferencing, telepresence, audio visua...announced today that it has donated video conferen...
Breaking Medicine News(10 mins):Health News:Elekta Family of Companies to Highlight Cancer Treatment Solutions at 2008 AAPM Annual Meeting 2Health News:Elekta Family of Companies to Highlight Cancer Treatment Solutions at 2008 AAPM Annual Meeting 3Health News:People With GERD More Likely to Develop Asthma 2Health News:Retina Group of New York To Present Seminar on Age-Related Macular Degeneration; The Leading Cause of Visual Loss in Seniors 2Health News:Retina Group of New York To Present Seminar on Age-Related Macular Degeneration; The Leading Cause of Visual Loss in Seniors 3Health News:Mt. Sinai's Children's Trauma Institute Treatment and Service Adaptation Center Receives Video Conferencing Donation From IVCi 2[0] Elekta Family of Companies to Highlight Cancer Treatment Solutions at 2008 AAPM Annual Meeting[0] People With GERD More Likely to Develop Asthma[0] Retina Group of New York To Present Seminar on Age-Related Macular Degeneration; The Leading Cause of Visual Loss in Seniors [0] Mt. Sinai's Children's Trauma Institute Treatment and Service Adaptation Center Receives Video Conferencing Donation From IVCi
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