Expression in Biological Technology

DNA2.0 Awarded US Patents for Breakthrough Gene Optimization Technology -- Company's Unique Algorithm Enables Expression Yields up to 50 Times Greater than Competing Approaches

The use of codon optimization in gene synthesis has long been misunderstood. DNA2.0 has cracked the code on optimizing sequence design and has been awarded two US patents covering the technology. DNA2.0's approach goes beyond codon arrangement, so the company is introducing a new, more accurate c...

GenomeQuest, Inc. Announces Digital Gene Expression Solution Within Its Sequence Data Management Platform

DigEx Solution offers completely interactive gene-level database to explore, understand, manage, and share next-generation sequencing DigEx runs. WESTBOROUGH, Mass., May 5 /PRNewswire/ -- GenomeQuest, Inc., the leader in sequence data management, today announced that it has released a Digital...

DNA2.0 Introduces Freedom of Expression With a New Family of Bacterial Expression Vect:ors

A significant challenge utilizing pET vectors for bacterial expression has been the cost and uncertainty associated with vectors that have been patented. In order to tackle this problem, DNA2.0 has developed a new family of vectors called pJexpress that are free of IP constraints. pJexpress vecto...

Lentigen and Expression Therapeutics Sign Hemophilia Co-Development Agreement

GAITHERSBURG, Md. and ATLANTA, Feb 4 /PRNewswire/ -- Lentigen Corporation, a biotechnology company specializing in the development and manufacture of lentiviral gene delivery technologies, and Expression Therapeutics, a biotechnology company specializing in factor VIII-based therapeutics, today an...

New technique allows simultaneous tracking of gene expression and movement

Flies expressing green fluorescent protein (GFP) in their retina cells or other tissues can be tracked by specially modified video cameras, creating a real time computer record of movement and gene expression. The new technique, described in the open access journal BMC Biotechnology , will allow ...

James L. Wittliff, Ph.D., FACB, to Present 'Predicting Breast Cancer Outcome with Gene Expression Signatures on the Ziplex(R) System' at the Association for Molecular Pathology Conference, October 30

Xceed Molecular will feature Ziplex automated gene-expression system at the conference, booth 519 WELLESLEY, Mass., Oct. 28 /PRNewswire/ -- Xceed Molecular, a pioneer in the development of cost-effective, easy-to-use gene-expression analysis systems, announced that renown...

Vascular Biogenics Ltd. Presented With the Frost & Sullivan 2008 European Gene Expression Targeting Platform Technology Innovation Award

MOUNTAIN VIEW, Calif., Aug. 26 /PRNewswire/ -- The 2008 European Gene Expression Targeting Platform Technology Innovation Award is presented to Israel-based Vascular Biogenics Ltd. for developing the Vascular Targeting System (VTS(TM)). This unique platform has facilitated novel advancements i...

ExonHit Launches Rat Genome SpliceArray(TM) Products to Expand High Resolution Gene Expression Profiling for use in Disease Modeling and Toxicogenomics

PARIS, May 6 /PRNewswire-FirstCall/ -- ExonHit Therapeutics S.A. (Alternext: ALEHT), a drug and diagnostic discovery company, announces today the availability of its new SpliceArray(TM) for use in disease modeling and toxicogenomic applications by the basic and pre-clinical research communitie...

Caliper Life Sciences Introduces High Throughput RNA Assay to Improve Workflow in Gene Expression Studies

- New assay assures integrity of RNA samples; offers improved throughput and walk-away time - HOPKINTON, Mass., Sept. 13 /PRNewswire-FirstCall/ -- Caliper Life Sciences, Inc. (Nasdaq: CALP ) today launched a new high throughput RNA assay for automatically assessing th...

Successful stabilization of gene expression profiles

RNA in a biological sample becomes extremely unstable upon collection of the sample, leading to changes in the gene expression profile. For accurate gene expression analysis in applications such as real-time RT-PCR and microarray analysis, it is important to stabilize the...

ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications

ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications Introduction ESR1 (Estrogen Specific Receptor 1) is a member of the nuclear hormone receptor (N...

New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays

Introduction Affymetrix has designed new algorithms for monitoring GeneChip ® expression data. These statistical algorithms, created in response to customer input, were designed to accommodate the typical distribution of data found in microarray experiments. The new stati...

Fine Tuning Your Data Analysis: Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms

Fine Tuning Your Data Analysis: Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms Introduction The Statistical Algorithms in Microarray Suite 5.0 process raw GeneChip probe set data to generate expression values (Signals and LogRatio...

Amplification Efficiency of TaqMan Gene Expression Assays

TaqMan Gene Expression Assays average 100% efficiency (+/- 10%). Abstract Applied Biosystems has designed and manufactured over 40,000 real-time PCR assays for measuring the expression of genes in humans, mice, and rats. TaqMan Gene Expression Assays each consist ...

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae : Detection in vivo using the Varian Cary Eclipse Paul Gavin# and Mark Prescott # , Ph.D Daren J. Fyfe, Ph.D * # Department of Bi...

Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

Serial Analysis of Gene Expression (SAGE) is a sequence-based approach to the identification of differentially expressed genes by comparative analyses. SAGE is a powerful tool, one of the more comprehensive methods available for detailed analysis of large numbers of cellular transcripts, ...

Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data

Introduction Microarrays offer a powerful means of studying human gene activity and thereby providing insight into cellular, molecular, and biological changes. Applied Biosystems has developed the Expression Array System, a complete system for gene expression studies. It incl...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

Sven Fraterman and Neal Rubinstein, Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia Abstract To locate gene products in distinct muscle fiber populations of different muscle allotypes, a novel protocol was establi...

A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays

Abstract This application note describes a method for extracting and isolating total RNA from rodent tissues that have been fixed in formalin and embedded in paraffin, and the subsequent amplification of mRNA from these tissues for use in microarray experiments. We have used this...

Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

Se-Jin Yoon and Kyung-Ah Lee Infertility Medical Center, CHA General Hospital, Seoul 135-081, Korea Abstract Laser Capture Microdissection (LCM) is an innovative technique which permits the rapid and reliable procurement of pure cell populations from heterogeneous tissue sec...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

(Anthony Lombardino, Rockefeller University) Abstract Brain tissue is notoriously heterogeneous, and accurate analysis of neural gene expression requires procurement of specific cell types. In this application note a technique is described for using Laser Capture Microd...

Microgenomic Expression Profiling

Abstract Microgenomic technologies enable expression array analysis from extremely limited cell mass. An integrated system of specialized separation techniques, advanced isolation and amplification protocols, and novel quality assurance checks is used in concert ...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

Dr. Eugene Trogan, Ph.D., Mount Sinai School of Medicine of New York University Abstract Macrophage foam cells are integral in the development of atherosclerotic lesions, however gene expression studies are complicated by the cellular heterogeneity of atherosclerotic plaque. This ap...

Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses

James R. Wong, Ph.D. and Wei-Yu Lin, Bio-Rad Laboratories Summary A double pulse format for electroporation of mammalian tissue culture cells gave lower transient expression than a single exponential decay pulse. Moreo...

Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System

Nicholas N. Lyssenko and Mark L. Tucker, Soybean and Alfalfa Research Lab, USDA, ARS, Bldg. 006, BARC-West, Beltsville, MD, USA 20705 Introduction In 1987 Jefferson et al. described the use of β-glucuronidase (GUS) as a marker to ...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

Stella Dracheva, Bronx VA Medical Center, Psychiatry Research, 130 West Kingsbridge Road, Bronx, NY 10468 USA Introduction Schizophrenia is a severe mental illness that afflicts about 1% of the population (Strange 1992). Patients ...

Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function

Iezzi M, Kouri G, Fukuda M, Wollheim CB (2004) Synaptotagmin V and IX isoforms control Ca 2+ -dependent insulin exocytosis. J Cell Sci 117:3119-3127. In a recently published paper in the ...

Your Data: Detection of Oocyte-specific Gene Expression Using Ambion's Cells-to-cDNA Kit

Ambion's Cells-to-cDNA Kit produces cDNA ideal for RT-PCR based gene quantitation, including experiments performed using real-time RT-PCR. The Cells-to-cDNA Kit comes in an easy-to-use format that produces first-strand cDNA from cultured ...

Unmask Blood RNA for Gene Expression Profiling

GLOBINclear-Human Globin mRNA Removal Kit Depletes alpha and beta globin mRNA from blood total RNA with a simple magnetic bead procedure (no enzymatic treatments) Increases sensitivity of gene ...

Total RNA from Whole Blood for Expression Profiling

RiboPure-Blood with RNAlater Provides Superior Stabilization and Recovery of RNA The RiboPure-Blood Kit recovers twice the RNA yield as the market leader's system RNA later Stabilization Reagen...

siRNA Expression Vectors with Selectable Markers

For Long Term Studies in Mammalian Cells Six new p Silencer siRNA expression vectors are now available, each with an antibiotic resistance gene to facilitate selection in mammalian cultured cells. These ne...

RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis

RNA later Tissue Collection: RNA Stabilization Solution is the worldwide standard for the preservation of RNA in intact animal tissues, organs, and cells. RNA later eliminates the need to immediately process tissue sam...

Preserve Samples for RNA Expression Microarrays RNAlater Around the World

RNA later protects cellular RNA within specimens during sample collection and during shipping. Whether you are in the lab, in the OR, or out in the field, you can use this aqueous, non-toxic Tissue Collection:RN...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

Ambion's p Silencer 4.1-CMV plasmid vectors are designed for long-term gene silencing experiments in a broad range of cell lines. The vectors carry an RNA polymerase II-type CMV promoter (human ...

miRNA Expression in White Blood Cells

Emmanuel Labourier, Marianna Goldrick, Ambion, Inc. microRNAs (miRNAs) are endogenous, 21-24 nt RNAs that mediate post-transcriptional gene regulation by pairing with the 3' untranslated region of messenger RNAs and actin...

Ambion Offers Gene Expression Profiling Service

Ambion now offers a complete Gene Expression Profiling Service* for Affymetrix GeneChip Arrays. From basic data analysis to our Full Service offering, no project is too large or too small. The Full Service offering includes consultation with Ambion's team of ex...

Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification

Introduction As the human genome project is completed, microarray technology offers the potential to study the genomes complexity. This technology facilitates the direct ext...

High-throughput gene expression analysis to screen for anti-asthma drugs

Andreas Pahl, Uwe Oelmller,* and Susanne Ullmann * Department of Pharmacology, University of Erlangen, Erlangen, Germany * QIAGEN GmbH, Hilden Germany Drug library screening based on ...

Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes

New GeneConnection discovery microarrays provide more than 4,000 unique human cDNA sequences Stratagene introduces the first in a series of human cDNA microarrays on glass slides. These GeneConnection discovery microarrays II which are based on the ...

Mammalian Expression Vectors with Interchangeable Selectable Markers

Site-specific recombinase-based vector system Lisa Marsh Carsten Carstens Stratagene Stratagenes Exchanger system of eukaryotic expression vectors offers unparalleled convenience. The drug-resistance genes of these vectors can be exchanged without subcloning. Through s...