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Eppendorf in Biological Technology

New Brunswick Scientific Completes Merger Transaction with Eppendorf

EDISON, N.J., Sept. 24 /PRNewswire-FirstCall/ -- New Brunswick Scientific Co., Inc. ("NBS") (Nasdaq: NBSC ), and Eppendorf Group ("Eppendorf") announced today the completion of the previously announced merger transaction in which Eppendorf acquired NBS for approximately $110 million in an all...

Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

Lynn Jordan, James Batchelor, and Ed Alderman Applied Science and Technology Department, Caliper Life Sciences Inc., Hopkinton, MA 01748 Kelly M. Clark, Joseph A. Hensley and Robert P. Weisbein Brinkmann An Eppendorf Company, Westbury, NY Jen...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient Ulrich Genschel Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg ...

Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient

Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient Eng-Lai Tan and Choon-Kook Sam NPC Laboratory, Institute of Postgraduate Studies and Research, University of...

Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler

Peter Scheinert, PhD, Barbara Behrens, PhD, and Dietmar Kahle, PhD Peter Scheinert, PhD, Bernhard-Nocht-Institute for Tropical Medicine, Virology Dept., Bernhard-Nocht-Str. 74, D-20359 Hamburg former address: Universi...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient Ulrich Genschel Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg ...

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit Sonja Vorwerk and Barbro Patterson Sonja Vorwerk, Max Planck Institut fr Zchtungsforschung, Carl-von-Linn Weg 10, 50829 Cologne, ...

Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System

Martin Simon, Petra Albrecht, Helmut J. Schmidt, and Andrs Jarrin Martin Simon, Petra Albrecht, and Helmut J. Schmidt, University of Kaiserslautern, Germany, Department of Ecology Andrs Jarrin, Eppendorf AG ...

Efficient and Reliable PCR Setup Using Eppendorf MasterMix

Christian Rohrer and Lars-Erik Peter Christian Rohrer, Eppendorf AG, Germany Lars-Erik Peters, Eppendorf 5Prime, USA Introduction The reliability of a PCR* experiment is dependent on its sensitivity, ...

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

Katrine Verdun, Richard Rouse, and Gary Hardiman BIOGEM, Division of Biology, University of California San Diego, La Jolla, CA 92093-0349 e-mail: ghardiman@ucsd.edu www.biogem.ucsd.edu Introduc...

Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq

Kathryn L. Dudeck and Wendy A. Dustman University of Georgia, Department of Marine Sciences Introduction This application describes the use of the MasterTaq polymer...

Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel

Christian Bauer, Oliver Klotzsche, and Frank Apostel Christian Bauer, Oliver Klotzsche, CCS Cell Culture Service GmbH, Falkenried 88, D-20251 Hamburg, Germany, Frank Apostel, Eppendorf AG, Germany ...

An Assessment of the Eppendorf twin.tec plate performance in PCR* applications

M. Arun Kumar, PhD, and Vincent Prezioso, PhD, Brinkmann BioSystems Laboratory Abstract To evaluate the performances of Eppendorf 96-well twin.tec PCR plates, we carried out PCR reactions with twin....

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit Joel Lopez, Melissa Higgins, Chere Griffin and Vince Prezios...

Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

... Probes (Cat.# O-11492) Black 96-well plate Brown eppendorf tubes Spectrofluorometer (Gemini XS, Gemini EM or SpectraMax ...ith OliGreen working solution into eleven brown (for protection from light) eppendorf tubes as described in the following table: Step 6: Add 100 L ...

Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)

...led water Heating block at 90-96˚C Plastic tubes (we used 1 ml eppendorf tubes) Black microplates (wall and bottom of plate) Gemini XS fluor...e NanoOrange working solution into eleven brown (for protection from light) eppendorf tubes as described in the following table: ...

Transformation of Nematodes With the Helios Gene Gun

...nd sonicate 3 times for 3 seconds in an eppendorf tube (1.5 ml). 2. Add 200 l of purifie...ively licensed to Bio-Rad Laboratories. eppendorf is a trademark of eppendorf Geratebau Netheler + Hinz, GmbH. back t...

Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix

...and place in a sterile 1.5 ml screw cap eppendorf tube. 2. Add 1 ml of 1x Trypsin/EDTA (Gi...e of the PCR process requires a license. eppendorf is a trademark of eppendorf Geratebau netheler + Hinz, GmbH. back ...

Electrotransformation of E. coli With M13 DNA

... glycerol. The final cell pellet was resuspended in 2 ml cold 10% glycerol. Cells were distributed in 50 l aliquots into chilled eppendorf tubes and then immediately flash-frozen in a dry ice/ethanol bath. Frozen electroporation competent cells were stored at -70 C. ...

Genomic DNA Preparation from RNAlater Preserved Tissues

...sential to achieving adequate digestion. Place in a 2.0 ml eppendorf tube with 1.5 ml digestion buffer. Add...orm and invert rapidly (do not vortex) for 2 minutes in an eppendorf rack. Spin samples on high in a microcentrifuge for 10 min...

Your Data: RNAlater Stories

... Four 20 m thick sections are transferred while maintaining low temperatures into pre-cooled 2 ml eppendorf tubes. Sections are quickly thawed and treated with 50 l of RNAlater for 3060 seconds. ...

Uses and Applications of FuGENE 6 Transfection Reagent

...o the incubator. Warm serum-free DMEM (no additives) to room temperature. Aliquot 94-100 l into each of 6 tubes as indicated below. Use sterile eppendorf tubes or small sterile polystyrene tubes labeled: A (100 l) B (94 l) C (100 l) D (97 l) E (97 l) F (94 l), correspondin...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

... Scott Tarpinian eppendorf 5 Prime, Boulder, CO, USA ... DNA templates. 5 Materials eppendorf TripleMaster PCR System Invitrogen Ther...R System Genomic DNA isolated using the eppendorf Perfect gDNA Blood Mini Kit ...

TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

... Scott Tarpinian eppendorf 5 Prime, Boulder, CO, USA ...ase, that lack an editing function. The eppendorf TripleMaster PCR System solves both of ...ty. 5 Materials and Methods eppendorf TripleMaster PCR System Invitrogen Ther...

TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates

... Jennifer Halcome eppendorf 5 Prime, Boulder, CO, USA Introduction eppendorf provides a complete system for long-range PCR1 ana...DNA can be used in combination with the eppendorf Mastercycler gradient thermocycler and...

TripleMaster PCR System: Direct Comparison with Competitors

...Peters, Walter Gross, and Jennifer Diers eppendorf 5 Prime, Inc., Boulder, CO, USA ... This experiment was performed to compare the eppendorf TripleMaster PCR System with the long-range PCR products of leading competitors. eppendorf was compared to competitors T and R in ge...

The TripleMaster PCR System for three different amplifications

... Christian Rohrer, eppendorf AG Introductio... been required for different applications. The new eppendorf TripleMaster PCR System, however, is a un...50 l volumes. Amplification was carried out in the eppendorf Mastercycler. ...

Two-step Gradient PCR*

...f the reactions was performed using the eppendorf Mastercycler gradient (3), which provid...gle PCR tube. Reactions were carried out using the eppendorf Mastercycler gradient and eppendorf Taq Polymerase. Nine micro liters of each reaction...

PCR Optimization

... most stringent annealing temperature is a prerequisite for reliable results and efficient amplification of a specific target. The eppendorf Mastercycler gradient provides a gradient function, that in one single run evaluates up to 12 different annealing, elongation, or de...

Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes

...and Christian Rohrer Andrea Tanzer, Institute of Milk Hygiene, Milk Technology and Food Science, Austria Christian Rohrer, eppendorf AG, Germany Introduction Bacterial enteritides, which are triggered by contaminated foodstuffs, ...

The use of the Mastercycler gradient for single-cell PCR and preimplantation diagnosis

...: 25/27 (92%) cells showed amplification using the eppendorf Mastercycler gradient (Figure 3), while 2...ow the efficiency and accuracy of the new eppendorf Mastercycler gradient in single-cell PCR. To achie... this, we compared the results obtained with the eppendorf Mastercycler with our results previously ...

Normalizing cDNA libraries using the EppendorfThermomixer

...A cDNA library was synthesized with the eppendorf Mastercycler. To further examine these ... Two different normalization methods using the eppendorf Thermomixer (1) are described in the ...nd incubated for 12 hours at 68C in the eppendorf Thermomixer for rehybridization. ...

The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA

...ified chromosomal DNA.11,12 PCR was performed with a eppendorf Mastercycler gradient** as follows: 50 pmol of each primer, 2.5 ...ain reaction (PCR) process requires a license. ** The eppendorf Thermal Cycler is an Authorized Thermal Cycler and may be used w...

The use of TaqMaster PCR Enhancer

...versity of Illinois in Chicago, USA Vincent Prezioso, eppendorf Scientific, Inc., Westbury, NY, USA Introduction ...mpurities using a novel PCR enhancer. The eppendorf MasterTaq Kit contains the TaqMaster PCR enhancer that has been ...

Enhancing PCR* Efficiency with MasterTaq

...0 4: display RT/hsp27 5: MMuLV RT/hsp27 A: eppendorf MasterTaq 1u/50 l B: MBI Fermentas TAQ 1 u/50 l ...hain reaction (PCR) process requires a license. **The eppendorf Thermal Cycler is an Authorized Thermal Cycler and may be used w...

HotMasterAn innovative Hot Start/Cold Stop technology for better PCR* results

...and Vincent Prezioso, PhD George Halley, eppendorf 5 Prime, Inc., Boulder, CO, USA Vincent ...es or protein contamination. eppendorf has developed an innovative Hot Start technology. ...periments in this paper were designed to show that eppendorf Hot Start/Cold Stop and self-adjusting ...

cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR

...forementioned buffer incompatibilities, are great. eppendorf has designed a new kit with novel chemist...fication from a single cDNA pool. The new eppendorf cMaster RTplusPCR System enables the full range ... Materials and Methods eppendorf cMaster RTplusPCR System ...

U937

... O) Electroporation buffer eppendorf Hypoosmolar Electroporation Buffer (PH) ...atant. Note: The overall incubation time in the eppendorf Electroporation Buffer must not exceed 30 minutes to guarantee...

U-266

... O) Electroporation buffer eppendorf Hypoosmolar Electroporation Buffer (PH) ...atant. Note: The overall incubation time in the eppendorf Electroporation Buffer must not exceed 30 minutes to guarantee...

t-HUVEC

... Electroporation buffer eppendorf Electroporation Buffer with 200 mOsmol/kg ... Note: The overall incubation time in the eppendorf Electroporation Buffer must not excee...tion Buffer with 200 mOsmol/kg (= 42% eppendorf Hypoosmolar Electroporation Buffer + 58%...
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