Electrospray in Biological Technology

Fast Analysis of Phenoxyacetic and Phenoxypropionic Herbicides in Vegetable Matrix by Negative Ion Electrospray LC/MS/MS

P.Y. Communal and A. Royer, Girpa, Angers, France C. Schauber and T. Faye, Varian, Inc., France Introduction Phenoxy acids are used as herbicides in agricultural and domestic areas. These compounds have to be detected at trace level to ensure a safe drin...

Analysis of ()-11-Nor-9-Carboxy-Delta-9-THC in Urine by Negative Ion Electrospray LC/MS/MS

J. Beck and C. Schmidt, Varian, Inc. Introduction Marijuana is one of the most commonly used illegal recreational drugs in the United States. The compound that gives the high from smoking marijuana is tetrahydrocannabinol or THC. Toxicology, forensic, and clinical l...

Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS

Z. Yang and S. Sadjadi, Varian, Inc. Introduction Amphetamine drugs are often abused and misused in sports, in the work place, and by recreational users. Forensic, clinical, and doping laboratories are frequently asked to analyze for the presence of amphetamines in ...

Quantitation of Digoxin in Human Plasma Using Negative Ion Electrospray Ionization LC/MS

D. Garteiz, S. Din, and R. Morrow TexMS Analytical Services, Houston, TX Introduction A Varian 1200L LC/MS/MS system was used for quantitative measurement down to 15 picograms of the cardiac glycoside, Digoxin, in human plasma using Electrospray Ionizati...

Analysis Of Chloramphenicol by Negative Ion Electrospray LC/MS/MS

Z. Yang, Varian, Inc. Introduction Chloramphenicol (CAP) is a potent, broad-spectrum antibiotic and potential carcinogen used in humans only at therapeutic doses for treatment of serious infections. Its use in meatproducing animals, food producing insects, aquacultu...

Analysis of Phenolic Estrogen Mimics in River Water by Negative Ion Electrospray LC/MS/MS

Z. Yang, Varian, Inc. Introduction Bisphenol A (BPA) is a primary raw material used for the production of polyacrylates, epoxy resin, phenolic resin, and polycarbonate plastic. 4-Nonylphenol (4-NP) is a degradation product of alkylphenol ethoxylate detergents used i...

Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS

Z. Yang, Varian, Inc. Introduction Nitrofuran antibiotics are veterinary drugs. The use of these in food production is already banned in the United States, Australia, Canada, Japan, Singapore, Bangladesh, and the European Union because of a possible increased cancer...

First ASMS Hites Award for Outstanding Research Publication Presented to Prof. Alison Ashcroft for Her Use of Waters Synapt HDMS System to Explore Protein Complexes

...Society of Mass Spectrometry (JASMS). Prof. Ashcroft's paper titled, Monitoring Copopulated Conformational States During Protein Folding Events Using electrospray Ionization-Ion Mobility Spectrometry-Mass Spectrometry appeared in the December 2007 issue of JASMS and was co-authored by Waters scientists Kevin Gi...

Waters Introduces New SYNAPT G2 with Greater Power, Versatility and Speed Than Any Other High-End Mass Spectrometer

...umber of experimental options and research applications. These comprise the nanoACQUITY UPLC(R) and TRIZAIC(TM) UPLC inlets and ion sources including: electrospray (ESI), nanoflow ESI, atmospheric chemical ionization (APCi), combined ESI/APCi (ESCi), atmospheric solids analysis probe (ASAP), and atmospheric press...

HybridSPE(TM)-Precipitation Technology Wins SelectScience.net 2009 Scientists' Choice Award for Best New Separations Product in 2008

... One of the major causes of ion-suppression in bioanalysis is the presence of phospholipids during LC-MS or LC-MS-MS analysis in the positive ion electrospray mode (+ESI). Phospholipids are the second largest lipid component in biological matrices after triglycerides, and are typically present in extremely h...

Waters Corporation Continues to Meet Today's Challenges for Improved Laboratory Operations with New Products at Pittcon 2009

...ze volatile and semi-volatile compounds of low and intermediate polarity traditionally analyzed by dedicated GC/MS instruments. Switching from an electrospray ionization source in LC/MS/MS mode to the APGC source for GC/MS/MS takes less than five minutes and requires no tools. Once in place, the APGC source ...

LC-MALDI and MALDI-MS/MS for the Analysis of Complex Peptide Mixtures

... not intentionally supposed to be present in the sample. It is believed that the use of multiple (complementary) mass spectrometric techniques, e.g. electrospray LC-MS/MS, would result in the identifi cation of even more proteins. These results are very encouraging and demonstrate the potential of the LC-MA...

Protein Depletion for Plasma and Serum Proteomic Analysis

...almblad M, Axelsson O, Bergquist J: Explorative study of the protein composition of amniotic fluid by liquid chromatography electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. J . Proteome Res. 2004, 3 :884-889.

Analysis of a Verapamil Microsomal Incubation using Metabolite ID and Mass FrontierTM

...oxylations that can occur multiple times with closely spaced retention times. The ion source of the mass spectrometer was operated in the positive ion electrospray ionization mode. The HPLC separation was achieved using a 150 2.1 mm 5 μm BetaBasic 18 column (Thermo Electron) and the following HPLC gradient c...

Quantitation of Acrylamide in Food Samples on the Finnigan TSQ Quantum Discovery by LC/APCI-MS/MS

...al derivatization is not necessary prior to acrylamide analysis. To date, most LC/MS/MS methods for the assay of acrylamide have utilized an electrospray ionization (ESI) source for the production of acrylamide ions. 1-4 Yet it is well known that ESI-MS is problematic when highly aqueous solutions, suc...

Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

...cetonitrile containing 0.1 % formic acid Gradient: 45% B for 3 minutes Mass Spectrometer Ionization mode: Positive electrospray ionization (ESI) Capillary temperature: 350C Spray voltage: 4.5 kV Sheath gas: 45 units Sweep gas: 10 units ...

Quantitation of Oxybutynin in Rabbit Plasma Using the Varian 1200L LC/MS/MS System

...avesical administration (injection in the bladder) of the drug. Instrumentation Varian 1200L LC/MS/MS equipped with electrospray Ionization (ESI) source Materials and Reagents Oxybutynin chloride from Sigma-Aldrich, Catalog Number O2881. Diph...

Detection of Alprazolam and Temazepam in a Bovine Plasma Matrix Using the Varian 500-MS Ion Trap Mass Spectrometer

...and Temazepam (Restoril) were analyzed in a Bovine Serum Albumin (BSA) matrix, both before and after protein precipitation using Liquid Chromatography electrospray Ionization Mass Spectrometry (LC/ESI/MS/MS). The ability of the Varian 500-MS IT Mass Spectrometer to detect and accurately quantitate these drugs in ...

Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS

...S, the use of the built-in 6-port injection valve and the syringe pump. Together they can be used as a diverter valve to avoid potential damage to the electrospray interface by diverting salts away from the source and applying a make-up volume of solvent for maintaining optimum spray conditions. Refere...

High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS

...y liquid-liquid (L-L) extraction before analysis. The limits of detection were 0.05-0.15 ng/g animal tissue using a triple-quad mass spectrometer with electrospray ionization in a positive mode. The analyte quantitation and identification was performed according to European Union (EU) guidelines, using multiple r...

Simultaneously characterizing and quantifying chloramphenicol and its metabolite using LC/MS/MS

... Lee, U.S. Food And Drug Administration Laboratory Information Bulletin No. 4289, August 2002, Confirmation of Chloramphenicol Residues In Crawfish by electrospray LC/MS. ...

Efficient Primary Structure Elucidation of Disulfide-bridged Peptides

... The combination of Edman sequence analysis and electrospray tandem mass spectrometry provides accurate and det...C sequencing system Highly accurate electrospray tandem mass spectrometry for the elucidation of di...ino acid analysis and by tandem mass spectrometry. electrospray tandem mass spectrometry: Identification of the di...

The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists

...ey have to be determined in veterinarian samples like muscle, liver, retina, and urine. The coupling of High Pressure Liquid Chromatography (HPLC) and electrospray Ionization Tandem Mass Spectrometry (ESI-MS-MS) seems to be the analytical method of choice for such thermally labile and polar substances. Classical ...

Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping

...radiolabelled with phosphate. (2) More recently, a technique for the sequencing of proteins directly from polyacrylamide gels using electrospray in combination with tandem mass spectrometry has been developed. This allows the exclusive use of automated mass spectrometry techni...

Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer

... MS Conditions: Mass spectrometer: Thermo Finnigan TSQ Quantum Ionization mode: electrospray (ESI), positive ion Resolution: 0.7 Dalton Full Width Half Maximum on Q1 and Q3 Selected Reaction Monitoring: Paroxetine 33...

Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology

... ruggedness to withstand repeated analyses in these types of matrices. Ion Sweep technology in combination with orthogonal electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) probes has resulted in a dramatic reduction in API solvent ion ...

Structural Determination of Flavonoids Using MSn

...m of apiginin with the full-scan MS4 (579>433>271>) mass spectrum of Compound X. Conclusions electrospray ionization of an unknown compound provides a soft ionization technique allowing the generation of an intact pseudomolecular ion at m/z ...

Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn

...ally digested, the resulting peptide fragments are separated using HPLC and the peptides are identified by on-line MS analysis using electrospray ionization. Alternatively, fractions may be collected after the HPLC separation and analyzed off-line by matrix-assisted laser desor...

Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer

... Mass spectrometer: Thermo Finnigan TSQ Quantum Ionisation mode: electrospray (ESI), positive ion SRM: Clenbuterol 277.1 > 203.0 +/- 0.3 Da, 22 V Collision energy ...