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Electroporation in Biological Technology

Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses

James R. Wong, Ph.D. and Wei-Yu Lin, Bio-Rad Laboratories Summary A double pulse format for electroporation of mammalian tissue culture cells gave lower transient expression than a single exponential decay pulse. Moreo...

Optimizing Chemical Transfection and Electroporation of siRNAs

Rich Jarvis (Associate Scientist, Ambion, Inc.) Alan D. King (Vice President and CSO, Cyto Pulse Sciences, Inc.) Low transfection efficiency is the most frequen...

Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection

Dmitriy Ovcharenko (Research Associate II, Ambion, Inc.) Gene silencing using small interfering RNAs (siRNAs) has become a powerful method for studying gene function. Human primary cells ...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length Number of field pulses Ad...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length Number of field pulses Ad...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length Number of field pulses Ad...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length Number of field pulses Ad...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length Number of field pulses Adj...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length Number of field pulses A...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length Number of field pulses Adj...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. Protocols for Bacteria, Y...

Optimizing electroporation parameters

To ensure that maximum transfection rates are achieved, the electroporation parameters should be optimized for each new cell line. The guidelines listed below for determining the ideal electroporation parameters as simply and as ...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. Protocols for Bacteria, Y...

Optimizing electroporation parameters

To ensure that maximum transfection rates are achieved, the electroporation parameters should be optimized for each new cell line. The guidelines listed below for determining the ideal electroporation parameters as simply and as ...

Transgenic Zebrafish by Electroporation

Russell J. Buono and Paul J. Linser, Whitney Marine Laboratory, University of Florida, 9505 Ocean Shore Blvd., St. Augustine, Florida 32086, 904-461-4000 The production of transgenic animals has proven to be a powerful tool in the study o...

Transformation of Filamentous Fungi by High-Voltage Electroporation

Contributed by G.H. Goldman, R. Geremia, M. Van Montagu, and A. Herrera-Estrella, Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium Introduction Virtually all fungal transformation protocols call for the addition of ...

Production of Hybridomas by Electroporation

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High Throughput siRNA Electroporation

NEW siPORTer-96 Electroporation Chamber Efficient siRNA delivery with minimal cell mortality Electroporate up to 96 samples at the same time Deliver siRNA into primary, ...

ImmunoVaccine Technologies Partners With Scancell to Advance DNA Vaccine Delivery

... for certain infectious diseases where alternative delivery methods such as electroporation may be less suitable." commented David Evans, chairman of Scancell. DNA-b...eveloped for the treatment of melanoma and will enter clinical trials using electroporation technology in early 2010. This press release contains forward-looking ...

Hebrew University scientist develop technique for eliminating reblockage of arteries

...rkeley. The technique employs the biophysical phenomenon of irreversible electroporation (IRE). IRE destroys cells within seconds, using very short electric field p... other structures in the treated area. While the phenomenon of irreversible electroporation was known for decades, a team led by Prof. Rubinsky developed a new mode of...

Vista Partners Updates Coverage on Inovio Biomedical Corporation (Amex: INO); $3.57 Price Target

... Inovio Biomedical (Amex: INO ) is focused on developing multiple DNA-based immunotherapies. Inovio is a leader in developing human applications of electroporation using brief, controlled electrical pulses to increase cellular uptake of a useful biopharmaceutical. Interim human data has shown that Inovio's DNA de...

Vista Partners Updates Coverage on Inovio Biomedical Corporation (Amex: INO) With $3.52 Price Target

...: Inovio Biomedical (AMEX: INO ) is focused on developing multiple DNA-based immunotherapies. Inovio is a leader in developing human applications of electroporation using brief, controlled electrical pulses to increase cellular uptake of a useful biopharmaceutical. Interim human data has shown that Inovio's DNA de...

Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer

...ch Laboratories, Inc.) was transfected into Jurkat cells using a Gene Pulser II system with the Capacitance Extender Plus. The electroporation was conducted with a 0.4 cm cuvette with 310 volts and 950 F using 10 g of linearized pEGFP-n2 vector. 4 Forty-eight hours post...

Electroporation of Yeast Artificial Chromosomes

... reasonable efficiencies with larger YACs. Several electroporation protocols have recently been reported f...ly used YAC cloning procedure, we investigated the electroporation of S. cerevisiae strain AB1380 (Mat a, ... reported in a previously published YAC electroporation protocol. 7 Materials and ...

Examples of Electro-Transfected Eukaryotic and Prokaryotic Cells

...onstruction M13 DNA Plasmid incompatibility RNA Release of cell components Use of more than one electroporation unit Experimental conditions available as an Electroprotocol back to top ...

Electroporation of Primary Bone Marrow Cells

...78B1/6 mice, washed, and resuspended in electroporation medium, at 7-9 x 10 6 cells/ml. Sample... (v/cm) and pulse time constant. We found that electroporation conditions of 625-750 V/cm and time con... cells. With phosphate buffered sucrose electroporation medium, the 25 F capacitor was used to ...

Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

...ains were transformed with 1 l of 10 pg/l of various plasmids by electroporation (Bio-Rad Gene Pulser electroporator), and plated to LB plates ...a standard lithium acetate protocol (Gietz and Woods 1994) or by electroporation with 100 ng of plasmid. DNA sequencing was performed directly on...

High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures

...Introduction It has become clear that electroporation of E. coli results in significantly hig...s for directional cloning of the cDNA.) electroporation One l aliquots of the sample DNA were ...lcohol precipitation or dilution before electroporation in order to achieve optimal transformation ...

Electrotransformation of E. coli With M13 DNA

... 20 minutes before the addition of X-Gal and IPTG. electroporation proved to be the most efficient techniq... Materials and Methods electroporation Competent Cells Fresh streaks of XL1-B...ely flash-frozen in a dry ice/ethanol bath. Frozen electroporation competent cells were stored at -70 C. ...

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

... and transferred to ice-cold 0.2 cm gap Bio-Rad electroporation cuvettes. A single pulse was delivered ...stigated the relationship between plasmid size and electroporation efficiency in E. coli and found that ev...iciency. Rearrangements incurred during electroporation were analyzed at the level of restricti...

Streamline Your siRNA Transfections

...s committed to bringing researchers the best solutions for siRNA delivery. Ambion's line of transfection and electroporation products offers consistent and reliable performance. siPORT NeoFX Transfection Agent is unique in that it ...

Setting up Successful siRNA Library Screens

... when using cell lines, or 5-10 electroporation conditions (e.g. varying cell... optimized for the reagent or electroporation condition that worked best among ...CatNum.php?8990">siPORT siRNA electroporation Buffer for more information. For ...

RNAi: A How To for New Users

... methodologies. In these cases, electroporation using a specialized, gentle-o...CatNum.php?8990">siPORT siRNA electroporation Buffer is a revolutionary new buffer that makes electroporation an ideal method for delivering ...

High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells

...ection Agent and siPORT siRNA electroporation Buffer, and an apparatus, the siPORTer-96 electroporation Chamber, to facilitate siRNA delivery in 96 .... Chemical transfection and electroporation are routinely used to deliver...

Deliver siRNAs Into Primary Cells

... siPORT siRNA electroporation Kit ... range of conditions Optimize electroporation of your cells quickly with in...CatNum.php?8990">siPORT siRNA electroporation Buffer overcomes this problem. It ...

Got Small RNA?

... this type of experiment. Here, GAPDH knockdown was triggered by electroporation of a GAPDH-specific siRNA in a primary human cell line (normal h...encer Negative Control #1 siRNA (NC, Ambion) in 400 ml of siRNA electroporation Buffer (Ambion). Total RNA and protein were isolated with the m...

Isolate It All: siRNA miRNA Total RNA Native Protein

... Control #1 (Ambion) in 400 l of siPORT siRNA electroporation Buffer (Ambion) using 4 mm electroporation cuvettes and square wave type pulses. T...DH knockdown was triggered by electroporation of a GAPDH-specific siRNA into a primary human ...

Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success

...tions must be identified and/or verified. The best way to test, optimize, and validate siRNA transfection or electroporation conditions is to use a highly effective, verified siRNA to an appropriate target and to monitor decreases in...

Delivering siRNAs to Difficult Cell Types

... electroporation of Primary, Neuronal and Other Hard-to-transfect C...ere we demonstrate the use of electroporation to deliver siRNAs into primary cells and other ... types. Ambion's siPORT siRNA electroporation Buffer can be used with commonly available ...

Five Ways to Produce siRNAs

...ib/tn/105/8.html">Optimizing Chemical Transfection and electroporation of siRNAs and (function() { var po = document.createElement('script'); po.type = 'text/javascript'; po.async = true; po.src = 'https://apis.google.com/js/plusone.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(po, s); })();