EcoR in Biological Technology

siRNA Design Guidelines

...the 3' end of the oligonucleotide. In addition, for cloning into the pSilencer 1.0-U6 vector, nucleotide overhangs to the ecor I and Apa I restriction sites are added to the 5' and 3' end of the DNA oligonucleotides, respectively (Figure 2). In con...

New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways

...ncoding residues 208 to 313 of rat c-Fos 12 into the BamH I and ecor I sites of pFA-CMV. Our results ( figure 3A ) in tran...idues 1-96 of human ATF2 14,15 was inserted into the BamH I and ecor I sites of the pFA-CMV vector. Consistent with previous reports,...

New Competent Cells for Highest Transformation Efficiencies

...nd the potential size bias, two plasmid vectors were used. Stratagenes pcmv-script vector (4.2 kb) was digested with Xho I and ecor I; Stratagenes two-hybrid pAD-GAL4 vector (7.2 kb) was also digested with the same two enzymes. A mouse brain cDNA library was p...

Easy Cosmid DNA Purification with StrataPrep Kits

...absent. The resulting cosmid DNA was digested to completion with ecor I ( Figure 1 ). ...smid DNA is completely digested with restriction enzymes such as ecor I ( Figure 2 ). 1 Fig.2 ...

High-Titer Retroviral Vectors for Gene Delivery

...ermination Fig.3 Coding sequence for the enhanced green fluorescent protein (GFP) 9 was inserted between the ecor I and BamH I sites in pFB and pFB-Neo. The 293 cell-based amphotropic retroviral producer cell line HW293-A (unpublished data) w...

Generate High-Quality, Directional Plasmid cDNA Libraries

...using Pfu DNA polymerase, 4 ligated to ecor I adaptors and digested with Xho I. Since ...mpatible overhang on its 3 end and an ecor I-compatible overhang on the 5 end. A...4-2.1 vector predigested with Xho I and ecor I (XR) restriction enzymes. Transformation ...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

...ific LIC-Right sequence. After standard second-strand synthesis, a double-stranded adaptor, the LIC-L-adaptor, which encodes the ecor I cohesive end and an LIC(L) sequence, is ligated to the double-stranded cDNA ends. The adaptor-ligated cDNA is then used as a t...

Versatile Reporter Vectors for Monitoring Viral Transduction

... seen for the humanized, red-shifted A. victoria GFP (EGFP) (data not shown). The coding sequence for hrGFP was inserted between the ecor I and Xho I sites in the pFB vector to produce the pFB-hrGFP vector (Figure 1A). The vector was then tested in retrov...

A New Lambda Vector for Mammalian Expression

...he Lambda ZAP-CMV vector predigested with either ecor I only or ecor I and Xho I, host strains, and helper phage. In ...e. We constructed a cDNA library in the ecor I and Xho I sites of the Lambda ZAP-CMV vector ...

Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries

...-selected cDNA was inserted between the ecor I and Xho I sites of the plasmid pFB ( ...assess the quality of both the phosphatase-treated ecor I/Xho I-digested vector as well as the ...zed. All of the 100 clones examined had ecor I/Xho I-excisable inserts, with an aver...

A Versatile Power Supply for All Electrophoresis Applications

...ure 3 , the SmartPower 4000 power supply was used for horizontal gel electrophoresis. The pBluescript vector was digested with ecor I and serially diluted. These samples were loaded onto a 1% agarose gel and electrophoresed for 1.5 hours at 100 volts in 1X TAE...