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EcoR in Biological Technology

siRNA Design Guidelines

...the 3' end of the oligonucleotide. In addition, for cloning into the pSilencer 1.0-U6 vector, nucleotide overhangs to the ecor I and Apa I restriction sites are added to the 5' and 3' end of the DNA oligonucleotides, respectively (Figure 2). In con...

New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways

...ncoding residues 208 to 313 of rat c-Fos 12 into the BamH I and ecor I sites of pFA-CMV. Our results ( figure 3A ) in tran...idues 1-96 of human ATF2 14,15 was inserted into the BamH I and ecor I sites of the pFA-CMV vector. Consistent with previous reports,...

New Competent Cells for Highest Transformation Efficiencies

...nd the potential size bias, two plasmid vectors were used. Stratagenes pcmv-script vector (4.2 kb) was digested with Xho I and ecor I; Stratagenes two-hybrid pAD-GAL4 vector (7.2 kb) was also digested with the same two enzymes. A mouse brain cDNA library was p...

Easy Cosmid DNA Purification with StrataPrep Kits

...absent. The resulting cosmid DNA was digested to completion with ecor I ( Figure 1 ). ...smid DNA is completely digested with restriction enzymes such as ecor I ( Figure 2 ). 1 Fig.2 ...

High-Titer Retroviral Vectors for Gene Delivery

...ermination Fig.3 Coding sequence for the enhanced green fluorescent protein (GFP) 9 was inserted between the ecor I and BamH I sites in pFB and pFB-Neo. The 293 cell-based amphotropic retroviral producer cell line HW293-A (unpublished data) w...

Generate High-Quality, Directional Plasmid cDNA Libraries

...using Pfu DNA polymerase, 4 ligated to ecor I adaptors and digested with Xho I. Since ...mpatible overhang on its 3 end and an ecor I-compatible overhang on the 5 end. A...4-2.1 vector predigested with Xho I and ecor I (XR) restriction enzymes. Transformation ...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

...ific LIC-Right sequence. After standard second-strand synthesis, a double-stranded adaptor, the LIC-L-adaptor, which encodes the ecor I cohesive end and an LIC(L) sequence, is ligated to the double-stranded cDNA ends. The adaptor-ligated cDNA is then used as a t...

Versatile Reporter Vectors for Monitoring Viral Transduction

... seen for the humanized, red-shifted A. victoria GFP (EGFP) (data not shown). The coding sequence for hrGFP was inserted between the ecor I and Xho I sites in the pFB vector to produce the pFB-hrGFP vector (Figure 1A). The vector was then tested in retrov...

A New Lambda Vector for Mammalian Expression

...he Lambda ZAP-CMV vector predigested with either ecor I only or ecor I and Xho I, host strains, and helper phage. In ...e. We constructed a cDNA library in the ecor I and Xho I sites of the Lambda ZAP-CMV vector ...

Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries

...-selected cDNA was inserted between the ecor I and Xho I sites of the plasmid pFB ( ...assess the quality of both the phosphatase-treated ecor I/Xho I-digested vector as well as the ...zed. All of the 100 clones examined had ecor I/Xho I-excisable inserts, with an aver...

A Versatile Power Supply for All Electrophoresis Applications

...ure 3 , the SmartPower 4000 power supply was used for horizontal gel electrophoresis. The pBluescript vector was digested with ecor I and serially diluted. These samples were loaded onto a 1% agarose gel and electrophoresed for 1.5 hours at 100 volts in 1X TAE...
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(Date:7/9/2014)... Bacteria found in the bladders of healthy women ... form of incontinence, according to researchers from Loyola ... These findings, published July 9, 2014, in the ... , suggest that bacterial communities may play a ... incontinence (UUI) is a common, yet poorly understood, ...
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(Date:7/9/2014)... risen dramatically in Western societies. One frequently cited reason ... have fewer infections than previous generations, thereby delaying maturation ... at Sahlgrenska Academy, University of Gothenburg, monitored children until ... immune system in relation to allergic disease. All of ... Gtaland Region, half of them on farms that produced ...
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(Date:7/8/2014)... discovered a biomarker that could give expecting mothers and ... predict that a pregnant woman may develop preeclampsia, at ... , Preeclampsia is a cardiovascular disorder generally occurring late ... creating immediate and potentially lifelong risks to both mother ... in the urine, and is typically diagnosed in the ...
Breaking Medicine News(10 mins):Health News:Study finds kidney donation safe for healthy older adults 2Health News:DARPA selects Lawrence Livermore to develop world's first neural device to restore memory 2Health News:DARPA selects Lawrence Livermore to develop world's first neural device to restore memory 3Health News:Health-care worker hand hygiene rates increase three-fold when auditors visible 2Health News:Health-care worker hand hygiene rates increase three-fold when auditors visible 3Health News:UI researchers find early predictor for preeclampsia 2Health News:UI researchers find early predictor for preeclampsia 3
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