Navigation Links
EcoR in Biological Technology

siRNA Design Guidelines

...the 3' end of the oligonucleotide. In addition, for cloning into the pSilencer 1.0-U6 vector, nucleotide overhangs to the ecor I and Apa I restriction sites are added to the 5' and 3' end of the DNA oligonucleotides, respectively (Figure 2). In con...

New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways

...ncoding residues 208 to 313 of rat c-Fos 12 into the BamH I and ecor I sites of pFA-CMV. Our results ( figure 3A ) in tran...idues 1-96 of human ATF2 14,15 was inserted into the BamH I and ecor I sites of the pFA-CMV vector. Consistent with previous reports,...

New Competent Cells for Highest Transformation Efficiencies

...nd the potential size bias, two plasmid vectors were used. Stratagenes pcmv-script vector (4.2 kb) was digested with Xho I and ecor I; Stratagenes two-hybrid pAD-GAL4 vector (7.2 kb) was also digested with the same two enzymes. A mouse brain cDNA library was p...

Easy Cosmid DNA Purification with StrataPrep Kits

...absent. The resulting cosmid DNA was digested to completion with ecor I ( Figure 1 ). ...smid DNA is completely digested with restriction enzymes such as ecor I ( Figure 2 ). 1 Fig.2 ...

High-Titer Retroviral Vectors for Gene Delivery

...ermination Fig.3 Coding sequence for the enhanced green fluorescent protein (GFP) 9 was inserted between the ecor I and BamH I sites in pFB and pFB-Neo. The 293 cell-based amphotropic retroviral producer cell line HW293-A (unpublished data) w...

Generate High-Quality, Directional Plasmid cDNA Libraries

...using Pfu DNA polymerase, 4 ligated to ecor I adaptors and digested with Xho I. Since ...mpatible overhang on its 3 end and an ecor I-compatible overhang on the 5 end. A...4-2.1 vector predigested with Xho I and ecor I (XR) restriction enzymes. Transformation ...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

...ific LIC-Right sequence. After standard second-strand synthesis, a double-stranded adaptor, the LIC-L-adaptor, which encodes the ecor I cohesive end and an LIC(L) sequence, is ligated to the double-stranded cDNA ends. The adaptor-ligated cDNA is then used as a t...

Versatile Reporter Vectors for Monitoring Viral Transduction

... seen for the humanized, red-shifted A. victoria GFP (EGFP) (data not shown). The coding sequence for hrGFP was inserted between the ecor I and Xho I sites in the pFB vector to produce the pFB-hrGFP vector (Figure 1A). The vector was then tested in retrov...

A New Lambda Vector for Mammalian Expression

...he Lambda ZAP-CMV vector predigested with either ecor I only or ecor I and Xho I, host strains, and helper phage. In ...e. We constructed a cDNA library in the ecor I and Xho I sites of the Lambda ZAP-CMV vector ...

Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries

...-selected cDNA was inserted between the ecor I and Xho I sites of the plasmid pFB ( ...assess the quality of both the phosphatase-treated ecor I/Xho I-digested vector as well as the ...zed. All of the 100 clones examined had ecor I/Xho I-excisable inserts, with an aver...

A Versatile Power Supply for All Electrophoresis Applications

...ure 3 , the SmartPower 4000 power supply was used for horizontal gel electrophoresis. The pBluescript vector was digested with ecor I and serially diluted. These samples were loaded onto a 1% agarose gel and electrophoresed for 1.5 hours at 100 volts in 1X TAE...
Other Tags
(Date:6/23/2015)... Fla. , June 23, 2015 /PRNewswire/ ... management and authentication solutions, today announced enhanced functionality ... multi-factor authentication solution.  The enhancements build on ... of the DigitalPersona Altus platform and provide ... compatibility. In today,s environment of ...
(Date:6/18/2015)... Conn. , June 18, 2015 NXT-ID, Inc. ... authentication company focused on the growing mobile commerce market announces ... a featured segment on "Money on the Mark", scheduled to ... City June 20 th . ... will be from 7:00 to 8:00pm EST. NXT-ID, Inc.,s CEO ...
(Date:6/17/2015)... Germany , June 17, 2015 ... Standard: QIA) today launched new Investigator ® STR assay ... the United States . The new genetic ... key genomic markers (short tandem repeats or STRs) for DNA ... evaluate the quality of DNA in each sample, a novel ...
Breaking Biology News(10 mins):Crossmatch Releases Enhanced DigitalPersona Altus Advanced Authentication Functionality 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 3QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 2QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 3QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 4QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 5
(Date:7/1/2015)... ... July 01, 2015 , ... The 28th annual Charity Horse Show hosted by ... 2015, at 6 p.m. in Pugh Bourne Park, 343 Oakfield Road. The event will ... zoo, an Elvis impersonator, a silent auction and concessions. , Horses and riders from ...
(Date:7/1/2015)... ... July 01, 2015 , ... Convoy of Hope will partner ... Twin Cities on Saturday, August 1, at Spring Lake Park High School. , During ... than $1 million worth of goods and services including free food, health services, family ...
(Date:7/1/2015)... ... July 01, 2015 , ... RowdMap ... to newly released white paper: “Pro-actively Identifying the High-Cost Patient Population: Insights from ... and is available at: http://www.hcttf.org/resouces-tools . , The Healthcare Transformation Task Force ...
(Date:7/1/2015)... (PRWEB) , ... July 01, 2015 , ... Franklin University, ... announce Dr. Leslie King has been named Dean of the College ... Dean, Dr. King will promote and support the vision and mission of Franklin University ...
(Date:7/1/2015)... Luis Obispo, CA (PRWEB) , ... July 01, ... ... non-surgical bunion treatment, announces their big summer sale. Customers purchasing two or ... with promo code "Summer15". , Bunion Bootie temporarily corrects misaligned toes ...
Breaking Medicine News(10 mins):Health News:Convoy of Hope Hosts Community Event in the Twin Cities 2Health News:A Population Based Approach to Pay for Value: RowdMap, Inc. Contributes to Health Care Transformation Task Force White Paper 2Health News:A Population Based Approach to Pay for Value: RowdMap, Inc. Contributes to Health Care Transformation Task Force White Paper 3Health News:Franklin University Appoints Dean of College of Health & Public Administration 2Health News:Bunion Bootie, the #1 Non-Surgical Treatment for Bunion Pain, Announces Their Big Summer Sale 2
Other Contents