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The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature

DNA pol...

Production of Full-Length, Biologically Active MEK1WT and MEK1CA

Stratagene Cloning Systems, Inc. Stratagene has successfully expressed and purified recombinant, wild-typehuman MEK1 )using the ESP yeast pro...

cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR

RT-PCR has become an essential method that allows researchers to determine the presence, structure and level of expression of an mRNA transcript in the organism, tissue or cell of interest. The basic protocol involves the synthesis of a cDNA copy of all the mRNA present in the s...

Optimized Dengue RT-PCR

Institute of Health and Community Medicine, Universiti Malaysia Sarawak Optimization of annealing temperature for Dengue RT-PCR by using Eppendorf's Mastercycler gradient Reverse Transcriptase-Polymerase Chain Reaction...

TransMessenger Transfection Reagent

For transfection of cells with siRNA in RNAi procedures Tested for a...

Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent

QIAGEN GmbH, Hilden, Germany Primary neurons are extremely difficult to transfect, making genetic modification of neuronal cells by delivery of either siRNA or plasmid DNA challenging ( ). Recently, Krichevsky and Kosik report...

TransMessenger Transfection Reagent Handbook

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TransMessenger Transfection Reagent

For transfection of cells with siRNA in RNAi procedures Tested for a...

Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*

Center for Neurologic Diseases, Brigham and Womens Hospital, Harvard Medical School, Boston, MA, USA Genetic manipulations using neuronal cells are notoriously difficult. Therefore, efficient delivery of short interfering RNA (siRNA)...

A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)

β -lactamases are either plasmid or chromosomally encoded bacterial enzymes which hydrolyze β -lactam antibiotics. The majority of β -lactamases produced by clinical isolates are serine active site enzymes. One successful approach to combating the serine active site β -lactamases is the use of β -lactamase inhibit...

Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

Simon Lydford & Petra Averbeck, Molecular Devices Limited, U.K. The FlexStation scanning fluorometer integrates sensitive optics, fluid transfer and temperature control, making it ideal for kinetic, cell-based fluorometric assays, such as the measurement of intracellular calcium, membrane potential and intracellular pH. <...

New Sonic Foundry search engine finds words, phrases in video presentations

The Madison-based business is marketing the e...

Children's engages MedAssets for supply chain management

of Atlanta to implement supply chain management solutions. Children's Hospital is the second shareholder of Child Health Corp. of America (CHCA) to sign an agreement with MedAssets in recent months. The health system has a total annual supply spend of more than $9...

UW professors get grants to engage technology for course delivery

Cramer, professor Hussain Bahia, program assistant Carole Kraak and staff from Engineering M...

E-business consortium evaluates 12 desktop search engines

Can't find your files? Desktop search applications bring some of the same technology used by Internet search engines to your hard drive, but the market is getting more crowded. The University of Wisconsin-Madison evaluated a dozen desktop search tools for usability, accuracy, versatility, efficiency, security and enterprise readine...

NameProtects watchdog tool keeps eye on search-engine ads

MADISON Digital brand-protection company NameProtects search tools allow companies to make sure names they want to use are not already taken, or that their trademarks are not being used impr...

Technology as the engine of business innovation

Forget about asking whether technology drives business or business drives technology. Stop worrying about whether or not technology is strategic. Silence all the rattling about how superior this technology is to that technology. In technology, as with love, there are certain questions that if you have to ask, you probably already know and dont like the answer. A more satisfying line of...

FULL LENGTH INSERT SEQUENCING

Agencourt offers a full length insert sequencing service for large insert vectors such as BACs, cosmids and fosmids. Agencourt has several features that allow us to provide high quality results with rapid turnaround including:...

Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System

FastStart Taq DNA Polymerase, a chemically modified versionof Taq DNA Polymerase for hot start PCR, was introducedin 2000. Based on the feedback from many customers,FastStart Taq DNA Polym...

ArrayScript RT: Maximize Yields of Full-length cDNA

Ambion scientists have engineered a mutant form of M-MLV reverse transcriptase to improve production of full-length cDNA. This engineered reverse transcriptase, <A href="http://www.ambion.com/catalog/CatNum.php?2048">ArrayScript (patent pending), produces significantly higher yields o...

Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

MJ Research, Inc., South San Francisco, CA We present a real-time quantitative PCR (qPCR) protocol for the detection and quantitation of soy genetically modified to be resistant to the herbicide glyphosate. The assay utilizes SYBR Green...

Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System

MJ Research, Inc., South San Francisco, CA A two-color real-time quantitative PCR (qPCR) protocol was used on the MJ Research DNA Engine Opticon 2 system to genotype the CYP2D6*4 allele in 211patient samples. Two hydrolysis probes, one labe...

Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

Amplified fragment length polymorphism (AFLP) analysis is a genetic mapping technique that uses selective amplification of a subset of restriction enzyme-digested DNA fragments to generate a unique fingerprint for a particular genome. First developed for plant studies, AFLP analysis is used for a variety of applications, s...

In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate

Abnormalities in neurotransmission within the brain can lead to several disease states, including anxiety, depression or schizophrenia. Extensive research has been carried out to discover the mode of action of various transmitters, in order to understand and combat these diseases. In many cells, binding of the neurotransmitter to its recept...

FluoroMax multiwavelength capability

Simultaneously monitoring the levels of relevant metabolites and cell parameters is necessary to properly assess the effectiveness of drugs on living cells. The FLUOROMAX spectrofluorometer allows the user to monitor up to eight wavelength-pairs of emission and excitation wavelengths. Coupling this capability with th...

WARF questions relevancy of documents used to uphold patent challenge

refu...

New Urbanism: Community planning Feng Shui

The need to have a rebirth of the American municipality. The need to create a solid platform for economic...

Wisconsin biosciences rise to the challenge

Wisconsin b...

Biomedical engineering conference invites manufacturers

The University of Wisconsin-Madison already puts on an annual design event at the end of each spring semester, but this year...

Here's why Wisconsin's stem cell patents are being challenged

Not only does that make for appealing television drama, but it's pretty...

Patent office upholds challenge to WARF stem cell patents

Every WARF claim regarding the met...

Brand protection in the age of customer engagement

Brands of every sort - from the globally familiar to the local politician du jour - are being savaged, attacked, extolled, and venerated with a speed never seen before. The digital tools of our age provide numerous new channels for brand commentary, both positive and negative, and these multiplying channels place daunting new demands on corporate and political brand...

Re-engineering our economy to be sustainable and profitable

Today, sustainability still carries some linguistic baggage, but those same business execs might stick around to tal...

Risks and returns: Building a search engine marketing portfolio

Managing a search engine marketing campaign effectively is a lot like managing any investment portfolio: you want...

WARF challenges show need for patent reform

Specifically, the process of challenging patents, such as those held by WARF, needs streamlining....

Search engine optimization: What's the word?

Obtaining a desirable search engine ranking requires good information about the keywords and keyword phrases tha...

Are consumers confused by search engine ads?

Plaintiff JR Cigar is a discount cigar seller. GoTo.c...

Modine adapts military research to idling engines

When truck drivers traveling cross-country pull over to rest, many leave their engines idling to regulate the temperature of their sleeping cabs. With over 500,000 heavy-...

English as the lingua franca of a new age: Its more powerful than any law

For the first time, World Cup referees and their assistants will have to show proficiency in written and spoken English in order to be among the 44 officials taking part in soccers global tournamen...

Marketers advised to engage "search economy"

This year's conference, which is being held at , is dedicated to helping atten...