ECTR

A Versatile Power Supply for All Electrophoresis Applications

Douglas Drake Michelle Cayouette Lisa Filippone Bob LeSchofs Stratagene 4000 Power Supply Stratagene has developed the new SmartPower...

Principles and applications of electrical cell fusion

Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cance...

Optimizing electroporation parameters

To ensure that maximum transfection rates are achieved, the electroporation parameters should be optimized for each new cell line. The guidelines listed below for determining the ideal electroporation parameters as simply and as quickly as possible are adapted from Eppendorf Brochure...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Principles and applications of electrical cell fusion

Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cance...

Optimizing electroporation parameters

To ensure that maximum transfection rates are achieved, the electroporation parameters should be optimized for each new cell line. The guidelines listed below for determining the ideal electroporation parameters as simply and as quickly as possible are adapted from Eppendorf Brochure...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompar...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocomp...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompar...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer

Nicola Hughes, Jean-Jacques Dunyach, 1Biovail Contract Research,Toronto,Ontario,Canada; 2Thermo Finnigan,San Jose,CA,USA; 3Therm...

Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray Ion Trap Mass Spectrometry

Andreas Hhmer, Helen Tran, and William S. Hancock The data presented here can be acquired using any Thermo Finnigan LCQTMion trap mass spectrometer with MS...

Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer

Mark Churchill , John Henninge, 1 Thermo Finnigan, Stafford House, 1 Boundary Park, Boundary Way, Hemel Hempstead, HP2 7GE, UK; 2 Aker University Hospital, Hormone Laboratory, Section for Doping Analysis,Trondheimsv...

Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer

David Barnidge , Gargi Choudhary Neuromics, Inc., Minneapolis, MN Thermo Finnigan, San Jose, CA The data presented here was acquired using the Proteome...

Analyzing Trace-Level Impurities of a Pharmaceutical Intermediate Using an LCQ Deca Ion Trap Mass Spectrometer and the Mass Frontier Software Package

Mark R. Kagan , Carrie Liu 1Thermo Finnigan, Piscataway, NJ; 2Thermo Finnigan, San Jose, CA; 3Eisai Research Institute, Andover, MA The data presented here can be a...

Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer

Mark Churchill and Mark Harrison, Thermo Finnigan, Hemel Hempstead, UK The data presented here can be acquired using a Thermo Finnigan TSQ Quantum mass spectrometer...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

Michael Hepburn and Glenn A. Miller, Ph.D., Molecular Profiling Laboratory, Genzyme, Framingham, MA The search for sequence variations in genomic DNA is important in the study of genes that play a role in the development of cancer and a variety of other single and multiple gene disorders...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

K. Sestak, L. Kim, K.-O. Chang and L. J. Saif, Food Animal Health Research Program, Ohio Agricultural Research and Development Program, The Ohio State University, Wooster, OH 44691 Three major structural proteins of the porcine coronavirus, transmissible gastroenteritis virus (TGEV), membrane (M), nu...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

Martin A.O.H. Menke*, Axel Reinecke-Lthge*, Barbara Mllmann*, Angela Kellner, Jutta Lttges*, Gnter Klppel* * Department of Pathology and Department of Hematopathology, Christian- Albrechts University Kiel, Michaelisstrae 11, D-24105 Kiel, Germany. Introduction Genetic alterations/variations are...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

Terry H. Landowski, Ibrahim Buyuksal, and William S. DaltonH. Lee Moffitt Cancer Center and Research Institute, University of SouthFlorida, 12902 Magnolia Drive, Tampa FL 33612 Introduction Identifying of mutations within specific genes has become an important strategy in determining the diagnosis and prognosis f...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

Kristi M. Miller, Tobi J. Ming and Ruth E. Withler, Pacific Biological Station,Department of Fisheries and Oceans, Nanaimo, B.C. V9R 5K6, Canada Introduction We describe a rapid and sensitive method for the detection of nucleotide sequence variation which can be used for largescale screening of population...

Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System

Tochigi Cancer Center, Tochigi, Japan Introduction Denaturing gradient gel electrophoresis (DGGE) is one of the most consistently used mutation scanning methods. It has evolved enormously over the last decade to be widely used particularly in gene diagnostic l...

Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System

, and Jef Raus , 1 Dr. L. Willems-Instituut and 2 Limburgs Universitair Centrum, Universitaire Campus, B-3590 Diepenbeek, Belgium Introduction Phenylketonuria (PKU) is a hereditary disease that gives rise to elevated blood leve...

Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System

Bernd Eschweiler and Beate Kilb, Institute for Water Research, Schwerte, Germany Introduction Bacterial diversity in environmental samples is usually determined by a characterization of isolated strains. A problem for the analysis and characterization of microbial communities is the inability to culture...

Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

Evelyn McGown, Ph.D. and Anna Lam, B.S. Molecular Devices Corporation, 8/99 Modern assays for proteolytic activity typically use synthetic chromogenic or flu-orogenic peptide substrates. Enzyme selectivity is obtained by choosing a peptide sequence uniquely recognized by the catalytic site of a particular enzy...

LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)

Anne T. Ferguson, Ph.D. Molecular Devices Corporation This application note describes the use of the SPECTRAmax Viability/Cytoxicity Assay Kit for animal cells. This assay uses two fluorescent dyes, calcein AM (cal...

Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)

1 GFP (Green Fluorescent Protein) is an extremely stable protein of 238 amino acids produced by the jellyfish that fluoresces in the lower green region of the visible spectrum....

Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

October 1999 Molecular beacons are fluorogenic oligonucleotide probes developed by Kramerand associates to detect specific nucleic acids in homogeneous solutions. They are single-stranded oligonucleotides that exist in a hairpin shape because of thecomplementary arms at the ends which bind together to form a stem....

Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)

By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the NanoOrange Pro software from Mo...

Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

By Yan Zhang., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the OliGreen Pro software from...

PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

Protein concentration is commonly estimated by measuring the absorbance at 280 nm and calculating the concentration using the extinction coefficient of the protein. The procedure is feasible because extinction coefficients are typically obtained using standard cuvettes with a fixed 1 cm pathlength. Extinction-based protein assays can also...

Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)

Most spectrophotometric methods for inorganic phosphorus (Pi) analysis are based on the color formed upon the reduction of a phosphomolybdate complex. The classic (and still widely-used) method is that of Fiske and Subbarow , in which the reduction is carried out with sulfite and aminonaphtholsulfonic acid. Over the years, modi...

UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)

Molecular Devices Corporation. 7/99 In 1994, Molecular Devices introduced SPECTRAmax , the first microplate reader equipped with a monochromator, rather than bandpass filters, for wavelength selection. It was also the first microplate reader capable of measuring absorbance below 340 nm. These new features transformed the micropl...

Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)

Calibration of a dissolution apparatus is necessary to produce accurate results for dissolution testing of pharmaceutical dosage forms and to allow comparison between batches and between laboratories. USP requirements for calibration are given in the Apparatus Suitability Test in USP Each individual appa...

Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

Pipettors often fail to meet the manufacturers stated specifications and pipetting is a major source of laboratory error even when the pipettor is operating properly. Accuracy and precision specifications typically provided by pipettor manufacturers are obtained under ideal conditions. In the laboratory, performan...

measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

Cathy Olsen, Ph.D., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089....