Double

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

Lucia Martinez and Ario de Marco EMBL Protein Expression Core Facility, Meyerhofstr. 1, D-69117, Heidelberg, Germany Immobilized Metal ion Affinity Chromatography (IMAC) is an easy and reliable technique that allows the fast separation of His-tagged proteins from total lysates. There are several commercially available resins but all of them s...

Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses

James R. Wong, Ph.D. and Wei-Yu Lin, Bio-Rad Laboratories A double pulse format for electroporation of mammalian tissue culture cells gave lower transient expression than a single exponential decay pulse. Moreover, separate administration of the two components of the double pulse rev...

Merge Technologies' net loss doubles to $10.1M

While the first quarter loss was well below the $27.0 million, or $0.80 per share net loss reported in the fourth quarter of...

Burke would like to double Act 255 angel tax credits

State commerce secretary Mary Burke said this week that she would like Wisconsin to double the amount of tax credits available to angel investors under the Act 255 program. In an interview, Burke said funding increases would be contingent on the state's fiscal health, but she would like to double the tax credits from the $3 million allocated in the current biennial budget. Under Act 255,...

Sonic Foundry has net loss for '05 despite nearly doubled revenue

Revenues were $8.3 million, an 89 percent increase over the previous fiscal year, with unit sales up 74 percent, owing to multiple unit sales, repeat purchases, new product offerings and increased marketing efforts....

Double your productivity! Hype that damages technologists' reputations

Occasionally something so egregious happens that it must be addressed. I'm talking, of course, about the latest advertisement for Sharp products. What's got my steam vents working overtime is the line, "We doubl...

BoneCare doubles options and profits as fiscal year closes

BoneCare's fourth-quarter net income has been reported...

Health Care Costs Continue Double-Digit Pace, But May Start Moderating in 2004

U.S. health care cost increases continued to escalate at a double-digit pace in 2003, but there are indications that rate hikes may begin moderating as early as next year, according to global HR outsourcing and consulting firm Hewitt Associates (NYSE: HEW). For 2004, Hewitt is projecting a 12.6 percent average increase for employers, which is lower than 2003's 14.7 percent rate. Large c...

Double header for research dollars from Washington

Wisconsin has just scored a rare double-header with two research grants coming over the transom almost at once. The (WARF), has received a three-year, $1.7 million grant further the study...